Food safety in raw milk production: risk factors associated to bacterial DNA contamination

While human illness from milkborne pathogens may be linked to contamination of the product after pasteurization or improper pasteurization, such diseases are usually associated with consumption of raw milk or its by-products. Molecular biology tools were applied to investigate contamination by Listeria monocytogenes, Salmonella spp., some pathogenic strains of Escherichia coli, and Campylobacter jejuni in 548 raw milk samples from 125 dairy farms established in two regions from southern Brazil. Moreover, 15 variables were evaluated for their association with raw milk contamination levels, and the risk factors were determined by multiple regression analysis. Salmonella spp. were more frequently detected, followed by pathogenic E. coli. There was difference in contamination index between the regions, in which risk factors such as temporary cattle confinement, low milk production, low milking machine cleaning frequency, and milk storage area without tile walls were identified. The risk factors were specific to each region studied. Nevertheless, the data can be used to improve milk quality of dairy farms/herds with similar management practices.     

Retention during processing and bioaccessibility of β-carotene in high β-carotene transgenic cassava root

Cassava is a root crop that serves as a primary caloric source for many African communities despite its low content of β-carotene (βC). Carotenoid content of roots from wild type (WT) and three transgenic lines with high βC were compared after cooking and preparation of nonfermented and fermented flours according to traditional African methods. The various methods of processing all decreased βC content per gram dry weight regardless of genotype. The greatest loss of βC occurred during preparation of gari (dry fermentation followed by roasting) from WT and transgenic lines. The quantities of βC in cooked transgenic cassava root that partitioned into mixed micelles during in vitro digestion and transported into Caco-2 cells were significantly greater than those for identically processed WT root. These results suggest that transgenic high βC cassava will provide individuals with greater quantities of bioaccessible βC.     

Assessing the use of erosion modeling to support payment for environmental services programs

Purpose

Payment for environmental services (PES) has assumed increasing importance in discussions about sustainable development strategies. Many of the PES programs are based on water erosion control and the corresponding environmental and economic benefits generated in the basins where they are implemented. The main objective of this study was to show how erosion susceptibility models can support PES programs.

Materials and methods

The application of the Universal Soil Loss Equation (USLE) in the Sarandi Experimental River Basin (32.7 km²), located in the Federal District, Brazil, was used as a study case. Then a scheme for organizing knowledge about ecosystem services related to erosion control and water resources was performed. Considering the generated scheme, the USLE results, the land use map, and the water use in the region, we evaluated how erosion modeling could support PES programs.

Results and discussion

The results show that a large part of the study basin (90%) presents “low” susceptibility to erosion, which is significant in terms of the use and conservation of ecosystem services, as well as being a limitation regarding the need for the implantation of PES programs for erosion control. Incentives for maintaining the natural vegetation in areas with higher erosion susceptibility have the greatest potential to justify PES programs in the study basin, and the sanitation company is the potential payer for erosion control in the Sarandi River Basin.

Conclusions

The application of the USLE in a spatially distributed form proved to be an important support tool for land management and the implementation of PES policies.     

Comparison of intraocular pressure and pupil diameter after sedation with either acepromazine or dexmedetomidine in healthy dogs

Objective

To compare intraocular pressure (IOP) and pupillary diameter (PD) following intravenous (IV) administration of dexmedetomidine and acepromazine in dogs.