论农业机械化在新农村建设中的重要作用

以新农村建设带给农机化新的发展机遇为主线,结合武汉市以实施农村"家园建设行动计划"为抓手,建设社会主义新农村的实际,论述农业机械化在新农村建设中发挥的重要作用。     

Progress in RNA-binding protein HuR and its roles in development of hepatocellular carcinoma

RNA-binding proteins （RBP） are molecules with a variety of biological functions discovered in recent years. Among them, HuR is an important RBP, widely expressed in various tissues of the body, and is a member of the Hu/embryonic lethal abnormal vision （ELAV） protein family. It mainly affects the expression levels of target genes in the cells by regulating the stability and/or translation efficiency of the mRNA of the genes, thus participating in the regulation of cell life activities. In recent years, more and more studies on HuR have revealed its important role in inflammation and cancer. This review summarizes the functions of HuR and probes into the regulation of HuR functions. In addition, the roles of HuR in the occurrence and development of hepatocellular carcinoma （HCC） are also discussed, which provides important information for exploring the pathogenesis, biomarkers and therapeutic targets of HCC.     

Correlation between progression of atherosclerosis accelerated by emotional stimulation and imbalance of SDF-1/CXCR4 in mice

AIM To observe effects of emotional stimulation on expression of stromal cell-derived factor-1（SDF-1） and CXC chemokine receptor 4 （CXCR4） in plasma, platelets, aortas, hippocampus and bone marrow of apolipoprotein E gene knockout （ApoE^-/-） mice, and to reveal the possible mechanism of the aggravated atherosclerotic plaque vulnerability by emotional stimulation. METHODS Thirty 8-week-old male ApoE^-/- mice were randomly divided into normal control group, high fat group, and emotional stimulation group. Ten 8-week-old inbred C57BL/6J mice served as blank control group. After 12 weeks of intervention, the serum levels of SDF-1 and CXCR4 were investigated by ELISA. The protein levels of SDF-1 and CXCR4 in platelets, aortas, hippocampus and bone marrow were determined by Western blot. The pathological damage of aortas was observed by oil red O staining. RESULTS Compared with blank control group, normal control group and high fat group, the mice subjected to emotional stimulation showed more serious atherosclerosis in aortas detected by oil red O staining, and increased levels of SDF-1 and CXCR4 in the plasma and aortas were also observed （P<0.05）. The results of Western blot showed that the protein levels of SDF-1 and CXCR4 in platelets, aortas and hippocampus were increased in the mice subjected emotional stimulation, but the expression of SDF-1 and CXCR4 in the bone marrow was decreased （P<0.05）. CONCLUSION Imbalance of SDF-1/CXCR4 may be the key target by which emotional stimulation accelerates the progression of atherosclerosis.     

Expression of cathepsin C and tumor necrosis factor-α in human coronary artery and their relationships with coronary heart disease

AIM To explore the possible mechanism of cathepsin C （CTSC） and tumor necrosis factor-α （TNF-α） in coronary heart disease （CHD） by detecting the protein expression of CTSC and TNF-α in human coronary artery tissue. METHODS The coronary artery tissues from 52 cases of CHD and 25 cases of accidental death without any heart disease in the Forensic Judicial Expertise Center of Guizhou Medical University from October 2018 to December 2019 were collected as CHD group and control group, respectively. The coronary artery stenosis and intimal plaque formation were examined by histopathology, the protein expression of CTSC and TNF-α was determined by Western blot, and the intracellular expression of CTSC and TNF-α was analyzed by immunohistochemical staining. RESULTS The results of HE staining showed that the intima of coronary artery in control group was smooth, and no thickening or stenosis was observed. In CHD group, the intima thickened irregularly, atherosclerotic plaques formed, the intima became thinner and the lumen showed eccentric stenosis in varying degrees （P<0.05）. The results of Western blot showed that the expression of CTSC and TNF-α in CHD group was significantly higher than that in control group （P<0.05）. Immunohistochemical staining showed that both CTSC and TNF-α were expressed in the cytoplasm of foam cells, and their positive expression was significantly higher than that in control group （P<0.05）. The results of Pearson moment correlation analysis showed that there was positive correlation between the expression of CTSC and TNF-α in CHD （r²=0.743, P<0.05）. CONCLUSION The up-regulated expression of CTSC in coronary artery tissue may promote the expression of TNF-α and affect the occurrence and development of CHD.     

桃新品种‘秋露蜜’的选育

‘秋露蜜’是从‘国庆’桃实生后代中选出的浓甜晚熟桃新品种。平均单果重248.3 g,果面鲜红,果肉乳白色,甘甜,品质上等,平均硬度达15.30kg/cm²,可溶性固形物含量19.08%。在晋南地区,果实9月上中旬成熟,果实发育期162 d左右。离核。     

红心中华猕猴桃新品种‘桂红’的选育

‘桂红’是从野生中华猕猴桃后代中选育出来的猕猴桃新品种。平均单果重94.4 g。果实短圆柱形,果顶平或微凸;果皮黄绿色、薄,果毛残存、柔软,果斑黄褐色。果肉外缘金黄色,中轴白色,子房桃红色,呈放射状,肉质细,汁多,清甜,有香味,干物质含量17.39%,可溶性固形物含量16.70%,可溶性糖含量10.69%,可滴定酸含量1.56%,维生素C含量994.3mg/kg,含18种氨基酸,含包括硒在内的多种矿物质。在广西桂林地区,果实9月中下旬成熟。     

日光温室蔬菜智能化管理系统建模

随着精准农业的发展,智能化管理将在现代农业中广泛应用。针对智能化管理在日光温室蔬菜生产中尚鲜见报道的情况,该研究以日光温室蔬菜为研究对象,通过对日光温室环境特征的分析,设计一种基于无线传感器网络技术的低成本温室监测系统,用于监测温度、湿度、二氧化碳浓度等关键环境参数,并建立日光温室蔬菜系统,通过建模,建立不同子模块,实现蔬菜生产的智能化管理。     

宁夏荒漠草原不同土地利用方式对土壤活性有机碳的影响

以宁夏荒漠草原不同土地利用方式(耕地、弃耕地、草地和柠条地)的土壤为研究对象,测定了不同土层土壤有机碳和活性有机碳含量,分析了土壤活性有机碳占有机碳的比例,以期探寻不同土地利用方式对土壤活性有机碳的影响。结果表明:不同土地利用方式土壤总有机碳含量范围在1.78~6.17 g·kg^-1,具体表现为弃耕地>柠条地>草地>耕地。表层(0~5 cm)土壤有机碳含量显著高于深层(10~60 cm),说明荒漠草原土壤有机碳主要富集在表层;4种土地利用方式土壤易氧化有机碳含量为0.57~1.38 mg·g^-1,变化趋势具体表现为柠条地>草地>耕地>弃耕地,且柠条地在每个土层都高于其它3种土地利用方式;土壤可溶性有机碳含量范围为6.8~11.1 mg·kg^-1,变化趋势表现为草地>耕地>柠条地>弃耕地。土壤易氧化有机碳含量占有机碳的比例范围为13.7%~43.1%,大小排序为柠条地>耕地>草地>弃耕地;土壤可溶性有机碳含量占有机碳的比例范围为0.11%~0.48%,比例大小为耕地>草地>柠条地>弃耕地,耕地和草地中土壤可溶性有机碳含量占有机碳比例随着土壤深度的增加而减小,这与有机碳在耕地和草地中变化趋势一致。土地利用变化可以显著影响土壤活性有机碳的含量与分布,能够敏感的反映土壤碳库的变化,可以作为评价宁夏荒漠草原土壤质量和肥力的指标之一。     

款冬叶片总黄酮提取工艺优化及抗氧化活性研究

以款冬叶片为试材,采用微波辅助法提取总黄酮,在单因素试验的基础上,采用正交实验法优化款冬叶片总黄酮提取工艺,同时以分光光度法测定提取物对1,1-二苯基-2-三硝基苯肼自由基(1,1-diphenyl-2-picrylhydrazyl,DPPH·)、羟基自由基(·OH)和超氧阴离子自由基(O-·2)的清除作用。结果表明:款冬叶片总黄酮最佳提取条件为乙醇体积分数70%,微波提取时间3 min,微波功率500 W,料液比1∶15 g·mL^-1,在该条件下,总黄酮的提取率为0.91%。款冬叶片总黄酮的质量浓度与抗氧化活性呈一定的线性关系,当总黄酮质量浓度为0.069 8 mg·mL^-1时,对DPPH·、·OH和O-·2的清除率分别达84.21%、84.10%和68.32%,表明款冬叶片具有良好的抗氧化能力,可作为天然的抗氧化剂进一步的开发和利用。     

铁皮石斛人工繁育技术

以铁皮石斛商品瓶苗和盆栽苗茎段为试材,添加不同浓度6-BA、NAA,诱导茎段腋芽、原球茎和不定根,建立再生体系,快速繁殖铁皮石斛组培苗,以期为秦岭淮河一线以北地区人工繁殖铁皮石斛提供参考依据。结果表明:外植体宜采用70%酒精浸泡20 s,0.1%升汞浸泡8 min消毒;MS+6-BA 3.0~5.0 mg·L^-1+NAA 0.1~1.0 mg·L^-1适合腋芽分化和增殖,分化率92%~100%;1/2MS+6-BA 2.0 mg·L^-1+NAA 0.5 mg·L^-1+土豆泥15%适合原球茎诱导增殖,增殖倍数7.7;1/2MS+NAA 0.3 mg·L^-1生根率为100%;以松针土移栽,成活率83%。