全文获取类型
收费全文 | 1012篇 |
免费 | 109篇 |
专业分类
林业 | 74篇 |
农学 | 58篇 |
基础科学 | 1篇 |
138篇 | |
综合类 | 49篇 |
农作物 | 73篇 |
水产渔业 | 127篇 |
畜牧兽医 | 518篇 |
园艺 | 20篇 |
植物保护 | 63篇 |
出版年
2024年 | 2篇 |
2023年 | 15篇 |
2022年 | 33篇 |
2021年 | 58篇 |
2020年 | 57篇 |
2019年 | 67篇 |
2018年 | 62篇 |
2017年 | 65篇 |
2016年 | 67篇 |
2015年 | 46篇 |
2014年 | 59篇 |
2013年 | 70篇 |
2012年 | 66篇 |
2011年 | 77篇 |
2010年 | 43篇 |
2009年 | 42篇 |
2008年 | 38篇 |
2007年 | 51篇 |
2006年 | 28篇 |
2005年 | 25篇 |
2004年 | 21篇 |
2003年 | 21篇 |
2002年 | 21篇 |
2001年 | 8篇 |
2000年 | 20篇 |
1999年 | 12篇 |
1998年 | 7篇 |
1997年 | 4篇 |
1996年 | 1篇 |
1995年 | 3篇 |
1994年 | 2篇 |
1992年 | 3篇 |
1991年 | 1篇 |
1989年 | 2篇 |
1988年 | 1篇 |
1986年 | 1篇 |
1985年 | 4篇 |
1984年 | 2篇 |
1983年 | 1篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1980年 | 1篇 |
1978年 | 1篇 |
1974年 | 1篇 |
1973年 | 1篇 |
1972年 | 2篇 |
1971年 | 2篇 |
1970年 | 2篇 |
1969年 | 2篇 |
1967年 | 1篇 |
排序方式: 共有1121条查询结果,搜索用时 15 毫秒
11.
Faleiro L Miguel G Gomes S Costa L Venâncio F Teixeira A Figueiredo AC Barroso JG Pedro LG 《Journal of agricultural and food chemistry》2005,53(21):8162-8168
Antilisterial activities of Thymbra capitata and Origanum vulgare essential oils were tested against 41 strains of Listeria monocytogenes. The oil of T. capitata was mainly constituted by one component, carvacrol (79%), whereas for O. vulgare three components constituted 70% of the oil, namely, thymol (33%), gamma-terpinene (26%), and p-cymene (11%). T. capitata essential oil had a significantly higher antilisterial activity in comparison to O. vulgare oil and chloramphenicol. No significant differences in L. monocytogenes susceptibilities to the essential oils tested were registered. The minimum inhibitory concentration values of T. capitata essential oil and of carvacrol were quite similar, ranging between 0.05 and 0.2 microL/mL. Antioxidant activity was also tested, the essential oil of T. capitata showing significantly higher antioxidant activity than that of O. vulgare. Use of T. capitata and O. vulgare essential oils can constitute a powerful tool in the control of L. monocytogenes in food and other industries. 相似文献
12.
Ana Regueiras lvaro Huguet Tiago Conde Daniela Couto Pedro Domingues Maria Rosrio Domingues Ana Margarida Costa Joana Laranjeira da Silva Vitor Vasconcelos Ralph Urbatzka 《Marine drugs》2022,20(1)
Microalgae are known as a producer of proteins and lipids, but also of valuable compounds for human health benefits (e.g., polyunsaturated fatty acids (PUFAs); minerals, vitamins, or other compounds). The overall objective of this research was to prospect novel products, such as nutraceuticals from microalgae, for application in human health, particularly for metabolic diseases. Chlorella vulgaris and Chlorococcum amblystomatis were grown autotrophically, and C. vulgaris was additionally grown heterotrophically. Microalgae biomass was extracted using organic solvents (dichloromethane, ethanol, ethanol with ultrasound-assisted extraction). Those extracts were evaluated for their bioactivities, toxicity, and metabolite profile. Some of the extracts reduced the neutral lipid content using the zebrafish larvae fat metabolism assay, reduced lipid accumulation in fatty-acid-overloaded HepG2 liver cells, or decreased the LPS-induced inflammation reaction in RAW264.7 macrophages. Toxicity was not observed in the MTT assay in vitro or by the appearance of lethality or malformations in zebrafish larvae in vivo. Differences in metabolite profiles of microalgae extracts obtained by UPLC-LC-MS/MS and GNPS analyses revealed unique compounds in the active extracts, whose majority did not have a match in mass spectrometry databases and could be potentially novel compounds. In conclusion, microalgae extracts demonstrated anti-obesity, anti-steatosis, and anti-inflammatory activities and could be valuable resources for developing future nutraceuticals. In particular, the ultrasound-assisted ethanolic extract of the heterotrophic C. vulgaris significantly enhanced the anti-obesity activity and demonstrated that the alteration of culture conditions is a valuable approach to increase the production of high-value compounds. 相似文献
13.
Fagner Machado Ribeiro Mrio Lima Pedro Aurlio Tataíra da Costa Diego Machado Pereira Thony Assis Carvalho Tadeu Vilela de Souza Hortência Aparecida Botelho Fabyano Fonseca e Silva Adriano Carvalho Costa 《Aquaculture Research》2019,50(7):2004-2011
The objective of this work was to evaluate the association between morphometric variables and carcass characteristics in Pirapitinga. We used a thousand specimens of Pirapitinga with an average weight of 1,200 g, which were stunned, slaughtered, weighed, measured, and processed for morphometric and processing yield analysis, to obtain weights, carcass and fillet yields. Initially, the linearity of the variables was verified. Pearson's simple and partial correlation tests were performed between all metrics. Track analysis was performed considering the weights and yields of carcass and filet as dependent variables and the others as independent variables. Ridge regression models were used to eliminate the effects of multicollinearity among the independent variables. Observations showed that the simple correlations between body weights and yields were superior to the partial ones in terms of magnitude. The length and circumference of the specimens were the measures most linearly associated with weight, carcass weight and fillet with rib weight. As for carcass yield and fillet yield, linear correlation coefficients were low and not significant when associated with body weights. With the path analysis, we could observe significant positive correlations between the morphometric measurements for weight, carcass weight and fillet with rib weight. The standard length, body circumference and body circumference/body height ratio are the most correlated. The latter are the most important variables in the evaluation of body weights in Pirapitinga fish. They can serve as criteria for indirect selection in searching for fish with better carcass characteristics. As for fillet and carcass yields, the morphometric variables have not shown to be efficient for indirect selection. 相似文献
14.
15.
Steffan Edward Octávio Oliveira Cíntia Carol de Melo Costa João Batista Freire de Souza Jr João Paulo Araújo Fernandes de Queiroz Alex Sandro Campos Maia Leonardo Lelis de Macedo Costa 《Tropical animal health and production》2014,46(8):1413-1417
The amount of short-wave solar radiation willingly tolerated by lactating Holstein cows on pasture was determined in an equatorial semi-arid environment. The study was carried out on a dairy farm located in Limoeiro do Norte, CE, northeastern Brazil. The observed behaviours were as follows: grazing, under the sun, under the shade, standing, lying, ruminating, idling and wallowing in the water. The behaviours were recorded using instantaneous scan sampling at regular intervals of 15 min from 0600 to 1800 hours over 5 days. On all sampling days, the meteorological variables, including local short-wave solar radiation (R S-W, W m?2), were recorded. The R S-W data were divided into five levels. The sun exposure was more frequent under low (100 %) and moderately low (97 %) levels, when R S-W remained below 500 W m?2. The grazing was more intense under low (100 %) and moderately low (93 %) levels. Above 500 W m?2, the grazing time significantly decreased (11 %). The cows avoided grazing under high (0 %) and very high (0 %) levels, when R S-W exceeded 700 W m?2. The ruminating behaviour was more frequent under high (33 %) and very high (37 %) levels, in which the highest averages of R S-W were recorded (815 and 958 W m?2, respectively). The standing posture was more frequent under low (100 %) and moderately low (97 %) levels. Therefore, the critical R S-W level that motivates cows to stop grazing and seek shade was in the interval between 500 and 700 W m?2. 相似文献
16.
Liliana R. Leite-Martins Maria I.M. Mahú Ana L. Costa Ângelo Mendes Elisabete Lopes Denisa M.V. Mendonça João J.R. Niza-Ribeiro Augusto J.F. de Matos Paulo Martins da Costa 《Preventive veterinary medicine》2014
Antimicrobial resistance (AMR) is a growing global public health problem, which is caused by the use of antimicrobials in both human and animal medical practice. The objectives of the present cross-sectional study were as follows: (1) to determine the prevalence of resistance in Escherichia coli isolated from the feces of pets from the Porto region of Portugal against 19 antimicrobial agents and (2) to assess the individual, clinical and environmental characteristics associated with each pet as risk markers for the AMR of the E. coli isolates. 相似文献
17.
18.
Luiz Sergio Almeida Camargo Fernanda Queiros Costa Michele Munk Sabine Wohlres‐Viana Raquel Varela Serapio Bruno Campos Carvalho Paulo Henrique Campos Alex Cabral Vieira Luiz Altamiro Garcia Nogueira Joao Henrique Moreira Viana 《Reproduction in domestic animals》2019,54(10):1357-1365
This study investigated the influence of heat shock during in vitro maturation on embryo development following in vitro fertilization (IVF) or parthenogenesis (Part). Immature bovine cumulus–oocyte complexes were exposed to heat shock (41.0°C) during the first 12 hr of in vitro maturation (IVM), followed by 12 hr at 38.5°C. Control group consisted of in vitro maturation for 24 hr at 38.5°C. Oocytes were in vitro‐fertilized or activated with ionomycin and cultured in vitro for 192 hr post‐in vitro insemination or parthenogenetic activation (hpia). There was an interaction (p < .01) between temperature of IVM and method of oocyte activation (IVF or Part) for cleavage at 48 hpia. Heat shock had a negative impact (p < .01) on cleavage of IVF embryos, whereas no (p > .05) effect was found in the Part embryos. Embryo development towards blastocyst stage at 168 and 192 hpia decreased in both IVF and Part embryos derived from heat‐shocked oocytes. Heat shock increased (p < .05) the apoptotic index in Part blastocysts, but no effect (p > .05) was found in IVF counterparts. Heat shock also down‐regulated the expression of AQP3 (p < .01) and up‐regulated the expression of HSP70.1 (p < .01) in Part blastocysts, whereas it down‐regulated the expression of ATP1A1 (p < .05) in IVF blastocysts. In conclusion, the effects of heat shock during IVM on early embryo cleavage and blastocyst apoptosis are influenced by the method of oocyte activation and expression of some genes can be disturbed in embryos derived from heat‐shocked oocytes. 相似文献
19.
Gomes Pöppl Á Costa Valle S Hilário Díaz González F de Castro Beck CA Kucharski LC Silveira Martins Da Silva R 《Veterinary research communications》2012,36(1):81-84
Estrus cycle is a well recognized cause of insulin resistance in bitches. The insulin receptor (IR) as well as the insulin-like
growth factor-I receptor belong to the same subfamily of tyrosine kinase (TK) receptors. The objective of this study was to
evaluate basal TK activity in muscle tissue of bitches during the estrus cycle. Twenty-four bitches were used in the study
(7 in anestrus, 7 in estrus, and 10 in diestrus). Muscle samples, taken after spaying surgery to determine TK activity, were
immediately frozen in liquid nitrogen and then stored at −80°C until the membranes were prepared by sequential centrifugation
after being homogenized. TK activity was determined by Poly (Glu 4:Tyr 1) phosphorylation and expressed in cpm/μg of protein.
TK activity was significantly lower (P < 0.001) in the animals in estrus (104.5 ± 11.9 cpm/μg of protein) and diestrus (94.5 ± 16.9 cpm/μg of protein) when compared
with bitches in anestrus (183.2 ± 39.2 cpm/μg of protein). These results demonstrate, for the first time, lower basal TK activity
in the muscle tissue of female dogs during estrus and diestrus, which may represent lower insulin signaling capacity, opening
a new field of investigation into the molecular mechanisms of insulin resistance in dogs. 相似文献
20.
E. S. Pereira A. B. R. Carmo M. R. G. F. Costa A. N. Medeiros R. L. Oliveira A. P. Pinto M. S. S. Carneiro F. W. R. Lima A. C. N. Campos S. P. Gomes 《Journal of animal physiology and animal nutrition》2016,100(6):1090-1096
In this study, we evaluated the requirements of calcium (Ca), phosphorus (P), magnesium (Mg), sodium (Na) and potassium (K) for sheep hair growth. Experimental diets contained different levels of metabolizable energy [ME; 0.96, 1.28, 1.72, 2.18 and 2.62 Mcal/kg of dry matter, (DM), corresponding to 4.23, 5.64, 7.58, 9.61 and 11.55 MJ/kg DM]. The lambs' hair (n = 48) at 2 months of age presented an average body weight (BW) of 12.05 ± 1.81 kg. At the beginning of the experiment, eight animals were slaughtered as a reference group to estimate the initial empty body weight and body composition. Net mineral requirements (g/day) ranged from 0.73 to 0.71 g of Ca, 0.51 to 0.49 g of P, 0.026 to 0.026 g of Mg, 0.16 to 0.19 g of Na and 0.15 to 0.13 g of K for animals with a BW ranging from 15 to 30 kg and a daily gain of 100 g. The results of this study indicate that the net macromineral requirements for weight gain in Morada Nova lambs are different from the values commonly recommended by the Agricultural and Food Research Council. 相似文献