Caspase 3 inhibitor Ac-DEVD-CHO attenuates 10-hydroxycamptothecin-induced activation of NF-κB in Bcap37 cells

AIM:To investigate the role of caspase 3 inhibitor Ac-DEVD-CHO in caspase 3 signaling pathway and NF-κB activation induced by 10-hydroxycamptothecin (HCPT) in human breast carcinoma cells. METHODS:The cell growth inhibition was measured by MTT assay. Agarose gel electrophoresis was performed for detecting cell apoptosis. Western blotting was used for determining protein expression. DIG-EMSA was conducted to measure the DNA-binding activation of NF-κB. RESULTS:Caspase 3 inhibitor Ac-DEVD-CHO attenuated HCPT-induced apoptosis in human breast carcinoma. Ac-DEVD-CHO also suppressed the degradation of caspase 3 and IκBα,and arrested the activation of NF-κB. CONCLUSION:Caspase 3 inhibitor Ac-DEVD-CHO regulates the activation of caspase 3 and NF-κB,and attenuates apoptosis in Bcap37 cell line induced by HCPT.     

Effects of different chemotherapeutic agents on reversing the acquired resistance to TRAIL gene in DLD1 colon cancer cells

AIM:To evaluate effects of different chemotherapeutic agents on reversing the acquired resistance to TRAIL gene and clarify the involved mechanisms in DLD1-TRAIL/R colon cancer cells. METHODS:Human colon cancer cell line DLD1-TRAIL/R cells that were resistant to TRAIL-expressing adenovector (Ad/gTRAIL) were treated with Ad/gTRAIL combined with different chemotherapeutic agents. Then, the cell viability was measured by MTT method, and apoptotic signaling conditions, including activation of caspase-3 and caspase-8, expression of Bax and Bcl-XL, were measured by Western blotting analysis. RESULTS:In vitro data showed that several chemotherapeutic agents, including 5-fluorouracil (5-FU) and mitomycin c (MMC), overcome the acquired resistance to TRAIL gene in DLD1-TRAIL/R colon cancer cells. The combination of Ad/gTRAIL and 5-FU effectively suppressed tumor growth in vivo in subcutaneous tumors established from DLD1-TRAIL/R cells. Further data showed that treatment with the combination of Ad/gTRAIL and 5-FU or MMC led to enhance the activation of caspase-3. Moreover, MMC but not 5-FU induced overexpression of Bax gene that was sufficient to overcome the resistance to TRAIL gene in DLD1-TRAIL/R cells. CONCLUSION:Chemotherapeutic agents, such as 5-FU and MMC, overcome the acquired resistance to TRAIL gene in DLD1-TRAIL/R cells. The candidate mechanisms for MMC but not 5-FU to overcome this resistance might involve the induction of over-expressed Bax protein in DLD1-TRAIL/R cells.     

不同培养基对香菇菌丝体酯酶同工酶的影响

本文应用垂直板聚丙烯酰胺凝胶电泳,对在不同培养基上生长的香菇菌丝体进行酯酶同工酶分析。结果表明,不同培养基会导致香菇菌丝体酯酶同工酶酶谱产生差异。     

韩国2种夜蛾幼虫形态记述(鳞翅目)

记述了韩国夜蛾科重要害虫灰长须夜蛾Hermina tarsicrinalis (Knoch)和希饰夜蛾Pseudoips stylpha(Butler)老熟幼虫的形态特征,并提供了形态特征图.所有标本均保存在韩国江原大学校森林资源保护学科昆虫标本室.图2参5     

麻风树JcWRI1基因克隆及功能分析

WRI1是AP2类转录因子的成员,在植物生长发育和脂肪酸合成途径中起着重要的调控作用.通过RT-PCR技术从麻风树中克隆了1个AP2家族基因,将其命名为JcWRI1.JcWRI1基因开放阅读框全长1137 bp,编码378个氨基酸.表达模式分析结果表明,在麻风树种子胚中没有检测到JcWRI1基因的表达,然而该基因在麻风...     

钾镁水平对番茄苗期生长、根系形态及钾素吸收和生理利用效率的影响

采用水培方法,研究不同钾、镁水平对"天福冠露"和"毛粉802"两种番茄幼苗生长、根系特征及钾素吸收、利用效率的影响。结果表明,钾、镁水平(8 mmol/L、1 mmol/L)处理的植株鲜重、干重及钾、镁水平(4 mmol/L、4 mmol/L)处理的株高显著高于其他处理。随钾、镁水平升高,两种番茄植株的根长、"天福冠露"植株的根直径、根体积和分根数先减少后增加;番茄植株钾素吸收率显著提高,生理利用效率显著降低。钾、镁水平(8 mmol/L、1 mmol/L)处理的植株根长、根表面积、根直径及根体积显著高于缺钾处理。钾、镁水平(4 mmol/L、4 mmol/L)处理,两种番茄植株根长、根表面积、根体积和分根数均显著高于缺镁处理,"天福冠露"植株钾含量显著高于"毛粉802",而钾素生理利用效率显著低于"毛粉802"。与其它等钾处理相比,缺镁植株钾素积累量最低、生理利用效率最高,镁过量处理的植株钾素生理利用效率显著降低。     

我国主食馒头抗老化技术研究进展

从我国主食馒头产品保藏中存在的问题出发,分析了馒头抗老化保鲜技术的研究现状,综述了馒头抗老化保鲜剂、抗老化工艺及添加原辅材料等馒头保鲜技术的研究进展.     

阴山北麓不同林分类型土壤持水性能研究

为分析阴山北麓内蒙古武川县林地持水量和固持水土的能力,以当地柠条、油松、沙棘、油松×柠条混交林、沙棘×柠条混交林5种典型人工林林分类型为研究对象,测定了土壤物理性质、土壤水分入渗以及水分贮存特征值等指标。结果表明：不同林分类型的土壤物理特征存在显著差异（P＜0.05）。容重表现为沙棘最大（1.452 g·cm-3）,油松最小（1.042 g·cm-3）;非毛管孔隙度的排序为油松＞油松×柠条混交林＞沙棘×柠条混交林＞沙棘＞柠条。各林分达到稳渗的速率与非毛管孔隙度的排序相一致,各样地的稳渗速率在0.47~1.32 mm·min-1之间,土壤入渗回归方程拟合效果较好。只有油松纯林和油松×柠条混交林更接近考斯加柯夫公式中的系数1/2。混交林地比纯林地对水分的贮存能力高,而油松×柠条乔灌木混交林比沙棘×柠条灌木混交林的最大持水量高112.8 t·hm-2。在阴山北麓地带更适合种植油松×柠条类的乔灌木混交林,乔灌木混交林能更加有效地提高林地持水功能。     

蒸发皿蒸发量及主要气象因子变化分析——以桂林灌溉试验站为例

以广西桂林灌溉试验站1980-2003年气象要素实测资料为例,分析蒸发皿蒸发量及主要影响因子的变化特点和规律。采用距平百分率法、滑动平均法和Kendall秩次相关法分析了蒸发量、气温、降雨量、风速、日较差、相对湿度的变化特点。分析结果表明,桂林农田灌溉试验站1980-2003年的蒸发量、降雨量、日较差和相对湿度呈上升趋...