汕头市猪场猪瘟和猪禽流感血清学监测报告

为了了解和掌握我市猪场的猪瘟免疫情况及感染高致病性禽流感疫病的可能性，我所分别于2005年2月和4月两次对全市范围内各类型的养猪场免疫猪瘟的猪只采集血清941份，进行猪瘟血清抗体监测和猪高致病性禽流感疫情血清学监测。现将监测结果报告如下。     

羊驼皮肤中可溶性鸟苷酸环化酶的表达差异

采用免疫组织化学、实时荧光定量PCR和Western blotting对可溶性鸟苷酸环化酶(sGC)在棕色和白色羊驼皮肤中的表达差异进行了研究,以探讨sGC在羊驼皮肤中的作用。实时荧光定量PCR结果显示,sGC在棕色羊驼皮肤中的相对表达量较低,是其在白色羊驼皮肤中的表达量的0.16倍,且差异极显著(P<0.01);Western blot-ting结果显示,在羊驼皮肤总蛋白中含有与兔多克隆抗体发生免疫阳性反应的条带,在棕色皮肤中的反应阳性弱于白色皮肤,且差异极显著(P<0.01);免疫组织化学结果表明,sGC免疫阳性主要分布在白色羊驼皮肤毛囊的毛球上方细胞及细胞间质,而在棕色羊驼皮肤毛囊中,sGC免疫阳性主要分布在毛球底部和外根鞘细胞及细胞间质。结果提示sGC参与羊驼毛色形成。     

Surveillance of Parapoxvirus Among Ruminants in Virginia and Connecticut

In 2008, two deer hunters in Virginia and Connecticut were infected with a unique strain of pseudocowpox virus, a parapoxvirus. To estimate the prevalence of this virus, and in an attempt to define the reservoir, Parapoxvirus surveillance was undertaken between November 2009 and January 2010. 125 samples from four ruminant species (cows, goat, sheep and white‐tailed deer) were collected in Virginia, and nine samples from white‐tailed deer were collected in Connecticut. We found no evidence that the parapoxvirus species that infected the deer hunters is circulating among domesticated ruminants or white‐tailed deer. However, parapoxvirus DNA of a different parapoxvirus species, bovine papular stomatitis virus (BPSV), was detected in 31 samples obtained from asymptomatic cattle in Virginia. Parapoxvirus DNA–positive cattle originated from the same counties indicating probable transmission among animals. Molecular analysis identified BPSV as the parapoxvirus affecting animals. Asymptomatic parapoxvirus infections in livestock, particularly young animals, may be common, and further investigation will inform our knowledge of virus transmission.     

特高多花黑麦草在贵州的适应性研究

通过对特高多花黑麦草在贵州不同生态条件下的试验研究,结果表明:特高多花黑麦草在三个不同地区均能完成正常的生育期,且生长良好、适应性强、产草量高、营养价值丰富,具有良好的推广应用前景。     

无磷酸氢钙日粮对生长猪的影响研究

将48头杜×长×大三元杂交生长猪随机分为两组,分别饲喂正常磷水平日粮和无磷酸氢钙日粮,结果表明与正常磷水平日粮相比,无磷酸氢钙日粮可显著提高磷的表观消化率(P＜0.05),增加猪场的纯收入,减少粪中磷的排放以保护猪场周边的生态环境.     

Comparative analysis of proteomic profiles between endometrial caruncular and intercaruncular areas in ewes during the peri-implantation period

The endometrium of sheep consists of plenty of raised intercaruncular areas （IC）. In order to better understand aglandular areas called caruncular （C）, and intensely glandular the endometrium involved mechanisms of implantation, we used LC-MS/MS technique to profile the proteome of ovine endometrial C areas and IC areas separately during the peri-implantation period, and then compared the proteomic profiles between these two areas. We successfully detected 1740 and 1813 proteins in C areas and IC areas respectively. By comparing the proteome of these two areas, we found 170 differentially expressed proteins （DEPs） （P 〈 0.05）, functional bioinformatics analysis showed these DEPs were mainly involved in growth and remodeling of endometrial tissue, cell adhesion and protein transport, and so on Our study, for the first time, provided a proteomic reference for elucidating the differences between C and IC areas, as an integrated function unit respectively, during the peri-implantation period. The results could help us to better understand the implantation in the ewes. In addition, we established a relatively detailed protein database of ovine endometrium, which provide a unique reference for further studies.     

黏膜免疫佐剂的筛选——Ⅰ．组织学与免疫组织化学研究

动物体内存在黏膜免疫系统,黏膜结合淋巴组织是黏膜免疫应答的组织基础.机体的淋巴组织50%以上存在于黏膜免疫系统.黏膜免疫系统具有重要而独特的功能,是病原体入侵的最大门户,接触大量种类繁多的抗原,被认为是执行局部特异性免疫功能的主要场所.因此,黏膜疫苗的研制和应用具有巨大的前景.选择合适的黏膜免疫载体,以最有效的促进黏膜免疫应答的途径投送抗原,加强黏膜免疫的效力,减少病毒性疾病的发生以及为细菌性疾病的防治,开辟一条非抗生素的新途径.     

根据雏鸡血清酶活性对蛋鸡进行早期选种的研究

本研究测定了147只8、12周龄S220母雏血清碱性磷酸酶(AKP)、谷草转氨酶和乳酸脱氢酶活性,估测其遗传力及其与产蛋性能间的表型相关和遗传相关。结果表明,8周龄血清AKP活性遗传力为0.62,300日龄产蛋量遗传力为0.20,两者间的遗传相关为0.8245。利用8周龄血清AKP活性作为辅助性状对300日龄产蛋量进行间接选择,其间接选择效率为个体选择的1.4519倍,为家系选择的1.4837倍,为合并选择的1.1887倍。因此,8周龄血清AKP活性可作为蛋鸡早期选种血液生化指标之一。     

玉米胚芽粕质量安全现状与应用

随着现代生物技术在玉米深加工产业中的发展和应用,副产品的产量越来越大.玉米胚芽粕是一种以玉米纤维和蛋白质为主的高营养物质,生物效价PER值与WHO/FAO推荐值相近,近似于人奶和鸡蛋生物学价值,目前已用于育肥猪、反刍动物和家禽饲料添加.本研究对玉米胚芽粕的品质进行分析,以期将这一廉价的饲料资源进一步合理开发利用,取得更...