Aus der Vorratsschutz-Arbeit des Pflanzenschutzamtes Berlin. Tierische Schädlinge in Nahrungs- und Genußmitteln der Großlagerhaltung und Bekämpfungsmaßnahmen in den Jahren 1980–1986

Zusammenfassung Es wird über das Auftreten von 25 Vorratsschädlingen, darunter 17 Käfer- und 3 Mottenarten, in den Lägern der Bevorratungsabteilung des Senators für Wirtschaft und Arbeit, der sonstigen Großlagerhaltung sowie in den Vorräten privater Haushalte berichtet. Die häufigsten Schädlinge in der Großlagerei waren Brotkäfer (Stegobium paniceum L.) und Speichermotten (Ephestia elutella Hbn.). Für den Vorratsschutz interessante Fälle und besondere Arten werden ausführlicher behandelt. Außerdem wird ein Überblick über die Entwesungs- und Entseuchungsarbeiten gegeben, bei denen neben den Begasungsmitteln mit den Wirkstoffen Methylbromid, Phosphorwasserstoff und Blausäure auch Vorratsschutzmittel auf der Grundlage von Dichlorvos, Pyrethrum und Piperonylbutoxid in verschiedenen Anteilen eingesetzt werden.

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Stored products protection activities of the Plant Protection Office Berlin. Pests of foodstuffs and pest-control in warehouses from 1980 to 1986

It is reported about the appearance of 25 stored-product pests, within 17 species of beetles and 2 species of moths in warehouses of the Bevorratungsabteilung des Senators für Wirtschaft und Arbeit as well as in the other warehouses and private households. The drugstore bettle (Stegobium paniceum L.) and the warehouse moth (Ephestia elutella Hbn.) were the most common pests. Cases of special interest and particular species are described in detail. Also is given a survey about the pest-controlling measures carried out with poisongas as methylbromide, phosphine and hydrocyanic acid and with insecticides on basis of dichlorvos, pyrethrum and piperonylbutoxide.

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Mit einer Abbildung und 3 Tabellen     

Effects of Protease and Urea on a Granular Starch Hydrolyzing Process for Corn Ethanol Production

The dry grind process using granular starch hydrolyzing enzymes (GSHE) saves energy. The amount of GSHE used is an important factor affecting dry grind process economics. Proteases can weaken protein matrix to aid starch release and may reduce GSHE doses. Two specific proteases, an exoprotease and an endoprotease, were evaluated in the dry grind process using GSHE (GSH process). The effect of protease and urea addition on GSH process was also evaluated. Addition of these proteases resulted in higher ethanol concentrations (mean increase of 0.3–1.8 v/v) and lower distillers' dried grains with solubles (DDGS) yields (mean decrease of 1.3–8.0% db) compared with the control (no protease addition). As protease levels and GSHE increased, ethanol concentrations increased and DDGS yields decreased. Protease addition reduced the required GSHE dose. Final mean ethanol concentrations without urea (15.2% v/v) were higher than with urea (15.0% v/v) in GSH process across all protease treatments.     

竹类对真菌的感受性

本文对来自几个不同国家竹类茎秆的霉菌和担子菌进行分离和DNA测序鉴定。对蓝变真菌的纯培养的样品的实验室染色试验表明,正如发生在木材细胞组织中的一样,密集的褐变菌丝、厚垣孢子对组织的侵染和木质化细胞壁的渗透压扭作用。利用不同的测试安排的纯培养的白变、褐变和软腐真菌的退化实验表明,相当大的质量损失是由于白化菌和软腐菌引起的。本研究通过透射电镜证实了真菌攻击的微观形态学表现。研究结果改变了真菌破坏竹子的基本看法,需要改进竹资源更好利用和更适当保护的措施。     

Effect of different dietary ratios of sunflower and linseed oils on growth and carcass traits of rabbits

As part of an experiment aiming to modify the meat fatty acid profile, this work studied the growth and carcass traits as affected by various dietary ratios of sunflower oil and linseed oil. A diet without added oil served as a control (C). Four other diets were equally 4% oil-enriched but they differed in the incorporation ratios of sunflower oil (S) to linseed oil (L), i.e. 4% S to 0% L (diet 4%S), 3% S to 1% L (diet 3:1%SL), 2% S to 2% L (diet 2:2%SL) and 0% S to 4% L (diet 4%L). The oil-rich diets had slightly higher digestible energy contents (11.4 vs 10.6 MJ/kg) than the C feed. In each group 10 litters of 7 to 9 Pannon White kits per litter were studied in the pre-weaning period from 21 to 35 days old. Growth and slaughter traits were assessed with 50 and 30 rabbits per group, respectively. No significant effects of diets were found on litter and doe performances. The only significant differences in growth performance of the C, 4%S, 3:1%SL, 2:2%SL and 4%L rabbits were for the 35–49 day feed intake (88, 86, 84, 84 and 83 g per day, respectively, P = 0.046), the 35–84 day growth rate (36, 38, 37, 35 and 37 g/day, P = 0.034) and the 84-day body weight (2608, 2703, 2664, 2565 and 2628 g, respectively, P = 0.022). There were several significant differences in carcass traits including the weight of reference carcass (1357, 1391, 1388, 1380 and 1369 g, respectively, P = 0.004) and left longisimus dorsi meat (78, 79, 81, 81 and 76 g, respectively, P = 0.046) of rabbits. The diets had major effects on the L*, a* and b* colour values (lightness, redness and yellowness) of meat and fat. Carcass colour of the C and 4%S rabbits was closer and the 4%L rabbits was further from the European consumer's preference of light coloured, less red and slightly yellow rabbit meat. Our result reveals the importance of age and body weight at slaughter. Taking the growth and slaughter performances and, the recent belief of human health benefits from lower n− 6/n− 3 FAs dietary ratios into account, the 2:2%SL diet seems most appropriate if the interests of the raisers, meat processors and buyers are considered equally.     

Seroprevalence of Anaplasma marginale in 2 Iowa feedlots and its association with morbidity, mortality, production parameters, and carcass traits

A prospective cohort observational study was conducted to investigate the seroprevalence of Anaplasma marginale in Iowa feedlots and its association with morbidity, mortality, and treatment costs. Blood samples were taken from 659 calves from 31 consigners at processing and classified as seropositive to A. marginale using a competitive enzyme-linked immunosorbent assay (cELISA) with a 30% cutoff. Health and production parameters were modeled by A. marginale serostatus with mixed model regression analysis. The apparent prevalence of seropositive cattle was 15.17% (100/659). When the cELISA positive cutoff was at 42% inhibition, the apparent prevalence was 5.00% (33/659). There was no significant association between A. marginale serostatus and production parameters; however, seropositive status had a weak positive association with undifferentiated fever (P = 0.17). Although prevalence of anaplasmosis in Iowa feedlots is higher than reported in Montana-sourced calves arriving in Canadian feedlots, this was not associated with increased production costs.     

Evaluation of PCR assays for the detection of Campylobacter fetus in bovine preputial scrapings and the identification of subspecies in South African field isolates

As a result of the high lability and slow growth of Campylobacter fetus subspecies, the laboratory diagnosis of bovine genital campylobacteriosis has always been difficult. This is especially true under South African conditions, where farms are far apart, laboratories are only present in major centres and there are high ambient temperatures. In order to overcome the shortcomings associated with traditional diagnostic methods, the implementation of a molecular assay was sought. This work describes how a previously published PCR assay (MG3F/ MG4R primers) was adapted, optimised and applied in the diagnostic laboratory to test preputial samples directly for the presence of Campylobacter fetus. Field evaluation of the assay revealed an analytical sensitivity and specificity of 85.7% and 99%, respectively. Subsequent genotyping and phenotyping of a diverse collection of South African field isolates revealed that South Africa has an unexpected and previously unreported high incidence of Campylobacter fetus subsp. venerealis biovar intermedius strains. These strains were not identified correctly by the subspecies-specific primer set evaluated. Until such time that cost- effective genotyping methods are available to diagnostic laboratories in South Africa, and other countries with these atypical Campylobacter fetus subsp. venerealis strains, the need for bacterial culture will persist. Identification to subspecies level of isolates at present remains dependent upon a single phenotypic criterion, namely tolerance to 1% glycine.     

Effects of compaction and water content on lodgepole pine seedling growth

Soil disturbance by heavy machinery usually causes a decrease in porosity and an increase in soil strength, which may limit access to nutrients and compromise seedling survival and growth. This study used a soil strength and a greenhouse experiment to assess the impact of compaction on lodgepole pine (Pinus contorta Dougl. Ex. Loud. var. latifolia Engelm.) seedling growth and the degree to which soil water influences the effects of compaction. A silt loam soil was collected from a forest landing in the central interior of British Columbia (BC) in the Sub-Boreal Spruce Biogeoclimatic zone. The silt loam soil was used in a soil strength experiment where soil with four water content levels (0.10, 0.18, 0.27, and 0.36 cm³ cm⁻³) was packed into 0.21 cm³ cores with three levels of compaction (74, 79, and 84% of maximum bulk density (MBD)). Soil strength was strongly affected by compaction and water content. In the greenhouse experiment, three water content levels (0.10–0.15, 0.20–0.30, and 0.30–0.35 cm³ cm⁻³) and three levels of compaction (67, 72, and 76% of MBD) were applied to soil in pots and 1-year old lodgepole pine seedlings were grown in the pots. Soil strength was highest (1275 kPa) for the high compaction and dry water content treatment in the greenhouse experiment. Though the soil strength for this treatment did not exceed 2500 kPa, the effect of compaction on growth was noticeable, with a decrease in diameter growth, total shoot mass, and new root mass as compaction increased at the dry water content. At dry water content and high compaction, foliar nutrient concentrations were greatest. Generally, water content had a greater impact on seedling growth than did compaction, at the levels of compaction used in this study. This study indicates that if there is a critical value for mechanical impedance of the conifer roots, it likely occurs below 2500 kPa. Our results are consistent with the explanation that soil strength incrementally affects root growth below 2500 kPa for this soil type. Expensive rehabilitation techniques may not be needed on lightly disturbed soils similar to that used in this study if soil water content is high enough throughout the conifer growing season to alleviate the effects of compaction on soil strength.