Effect of parasitism with Ostertagia circumcincta on pharmacokinetics of fenbendazole in sheep

Plasma and abomasal fluid concentrations of fenbendazole and its two major metabolites in sheep experimentally infected with Ostertagia circumcincta were compared with those in the same sheep when non-parasitised. Bio-availability of the drug was reduced in the parasitised state. There was also a reduction in the proportion of drug present in the form of metabolites in parasitised as compared with non-parasitised animals.     

The reaction of guaiacol with iron III and chromium VI compounds as a model for wood surface modification

Summary The reaction of ferric chloride with the lignin model guaiacol affords primarily a complex mixture of coupled guaiacol oligomers. Major components were the symmetrical carbon-carbon coupled dimer 3,3-dimethoxy-[1,1-biphenyl]-4,4diol and the trimer 3,3,5-trimethoxy-[1,1:3,1-terphenyl]-4,4,4-triol which were isolated by preparative HPLC and characterized by ¹HNMR spectroscopy and mass spectrometry. An unstable component believed to be a 4,4-diphenoquinone derived from the trimer was also prominent. The reaction of chromium trioxide with guaiacol yields the same dimer, trimer and diphenoquinone as well as 2-methoxy-p-benzoquinone. The major product with chromium trioxide, however, is an inert, highly insoluble polymer which was shown by degradation to contain guaiacol oligomers bound or crosslinked by hydroxylated chromium species. Magnetic susceptibility measurements clearly indicated that the valency of chromium in the polymer was + 3. It is postulated that similar complexes formed from phenolic lignin units are responsible for the weather resistance of chromium trioxide treated wood surfaces. In a broader context this study is relevant to the fixation of hexavalent chromium from a range of widely used wood preservative formulations.The authors wish to thank the following: Tom Syers (CSIRO Division of Forest Products) for technical assistance, Mary Reilly (CSIRO Division of Forest Products) for NMR spectral determinations and Ivan Vit (CSIRO Division of Chemicals and Polymers) for mass spectral determinations     

Surface deterioration of wood-flour polypropylene composites by weathering trials

The market for wood-fiber plastic composites (WPCs) is expanding rapidly in many countries including Japan, where WPCs are mainly used for exterior products. In such applications, WPCs undergo undesirable color change, chalking, and shrinkage and swelling, and accordingly there is a need to better understand the mechanisms responsible for the weathering of WPC and develop methods of improving their weathering resistance. In this study, weatherability of WPC was assessed by natural and accelerated weathering trials. Discoloration (whitening) of WPC during exposure was caused by degradation of both wood and plastic. Darker color pigments as additives improved the color stability of WPC; however, chalking on the surfaces still occurred. The color stability of WPC was improved by application of exterior coatings. Preweathering of WPC (before coatings were applied) increased the absorption of coatings by the WPC and had a positive effect on the color stability and prevented chalking of the composites. Parts of this study were presented at the IUFRO XXII World Congress Meeting, Brisbane, August 2005 and the IAWPS 2005, Yokohama, November 2005     

Damage caused by the American grey squirrel (Sciurus carolinensis) to agricultural crops, poplar plantations and semi-natural woodland in Piedmont, Italy

The incidence of damage caused by grey squirrels in the Turinplain was studied to assess the impact this alien species ishaving on the highly fragmented Piedmont environment, in particularon profitable cereals, poplar plantations and semi-natural woodlands.The survey of damage was conducted both by interviews to localfarmers and growers and by direct assessment using the NearestNeighbour Method on plantations and woodlands, studying randomlychosen plots in the Turin plain to gain a general overview ofthe situation. The results show a low incidence of damage causedby grey squirrels in the area: little or none was found in semi-naturalwoodlands and in agricultural crops, apart from maize whereless than 1 per cent of fields showed damage, and poplar plantationswhere 5 per cent had signs of bark stripping. Damage on maizecrops was random across the plain, but debarking damage to poplarswas mostly concentrated on the western side of the plain, andtended to occur only in particular years. Damage was concentratedclose to streams and rivers surrounding fields and plantations.The introduction of the grey squirrel in Italy has already causedecological damage such as the extinction of the red squirrelin some areas. Nevertheless, little or no information is availableon the damage this species is causing on woodlands or agriculturalareas. This study is a preliminary survey in order to fill thisgap.     

Soil carbon distribution and quality in a montane rangeland-forest mosaic in northern Utah

Relatively little is known about soil organic carbon (SOC) dynamics in montane ecosystems of the semi-arid western U.S. or the stability of current SOC pools under future climate change scenarios. We measured the distribution and quality of SOC in a mosaic of rangeland-forest vegetation types that occurs under similar climatic conditions on non-calcareous soils at Utah State University's T.W. Daniel Experimental Forest in northern Utah: the forest types were aspen [Populus tremuloides] and conifer (mixture of fir [Abies lasiocarpa] and spruce [Picea engelmannii]); the rangeland types were sagebrush steppe [Artemisia tridentata], grass-forb meadow, and a meadow-conifer ecotone. Total SOC was calculated from OC concentrations, estimates of bulk density by texture and rock-free soil volume in five pedons. The SOC quality was expressed in terms of leaching potential and decomposability. Amount and aromaticity of water-soluble organic carbon (DOC) was determined by water extraction and specific ultra violet absorbance at 254 nm (SUVA) of leached DOC. Decomposability of SOC and DOC was derived from laboratory incubation of soil samples and water extracts, respectively.

Although there was little difference in total SOC between soils sampled under different vegetation types, vertical distribution, and quality of SOC appeared to be influenced by vegetation. Forest soils had a distinct O horizon and higher SOC concentration in near-surface mineral horizons that declined sharply with depth. Rangeland soils lacked O horizons and SOC concentration declined more gradually. Quality of SOC under rangelands was more uniform with depth and SOC was less soluble and less decomposable (i.e., more stable) than under forests. However, DOC in grass-forb meadow soils was less aromatic and more bioavailable, likely promoting C retention through cycling. The SOC in forest soils was notably more leachable and decomposable, especially near the soil surface, with stability increasing with soil depth. Across the entire dataset, there was a weak inverse relationship between the decomposability and the aromaticity of DOC. Our data indicate that despite similar SOC pools, vegetation type may affect SOC retention capacity under future climate projections by influencing potential SOC losses via leaching and decomposition.     

The effects of within-species and between-species variation in wood density on the photodegradation depth profiles of sugi (Cryptomeria japonica) and hinoki (Chamaecyparis obtusa)

Low density wood is more rapidly eroded than denser wood when exposed to the weather, possibly because it is more susceptible to photodegradation. Fourier transform infrared microscopy was used to examine: (1) the depth of photodegradation in earlywood and latewood of sugi (Japanese cedar) and earlywood of hinoki (Japanese cypress) exposed for up to 1500 h to artificial sunlight emitted by a xenon lamp (375 W/m² within the 300 to 700 nm spectral range); and (2) the relationship between the density of wood tissues and depth of photodegradation. The depth of photodegradation varied between species (sugi and hinoki) as well as within a growth ring (sugi earlywood and latewood), and there was an inversely proportional relationship between depth of photodegradation and wood density. These findings may explain why low density earlywood is more rapidly eroded than latewood during weathering, and more generally, why there is an inverse relationship between the density of wood species and their rate of erosion during artificial and natural weathering. Part of this work was presented at the 54th Annual Meeting of the Japan Wood Research Society, Sapporo, August 2004     

Community‐based management of the environment: lessons from the past and options for the future

1. Traditional community‐based systems of managing coastal fisheries were (or in some cases are) successful in managing resources at sustainable levels. These systems are used as models in the present study. 2. They were (or are) under‐pinned by a sense of ownership and intimate knowledge of the environment in local people. 3. Such features have been largely lost, as subsistence economies have been replaced by capitalist ones, and environmental management responsibilities have shifted from local communities to national or international government. 4. There are, nevertheless, some examples of community‐based management systems that still operate successfully and we believe that modern management practices, in general, would benefit from increased community involvement. This will require renewed emphases on feelings of ownership and increased knowledge of the environment in the general public. 5. Environmental education will have a fundamental role to play in achieving this goal. However, its impact in schools and universities has been disappointingly ineffective so far in promoting a population that is sufficiently knowledgeable and well‐motivated to partake meaningfully in environmental management processes. 6. It is argued that educational initiatives, which involve all ages and sections of society, are required. Five promising possibilities are: (i) the participation of community groups in scientific projects; (ii) increased emphasis on life‐long learning; (iii) educational campaigns; (iv) citizens' juries; and (v) the involvement of community groups in environmental planning and management processes. 7. Wide‐scale adoption of innovations of these kinds will require funding and this will be achieved only by national re‐assessments of educational needs and priorities. Copyright © 2001 John Wiley & Sons, Ltd.     

Lipid Droplet Analysis Using In Vitro Bovine Oocytes and Embryos

The aim of this study was to quantify the content of lipid droplets in bovine oocytes and embryos from Bos indicus (Bi), Bos taurus (Bt) and Bos indicus × Bos taurus (Bi × Bt). Oocytes were aspirated post‐mortem and subjected to in vitro maturation, in vitro fertilization and in vitro development; the medium employed at each stage (TCM‐199, TALP, SOF) was supplemented with (i) serum replacement (SR), (ii) foetal calf serum (FCS) or (iii) oestrous cow serum (ECS). The structure and distribution of the lipid droplets were established using electron microscopy, but were quantified using an optical microscope on semi‐fine toluidine blue‐stained sections. The highest percentage of embryos corresponded to those produced with FCS and ECS, which differed from embryos generated with SR (p < 0.05). The highest percentage of morulae and the lowest percentage of blastocysts were obtained with the SR supplement (p < 0.05). The oocytes cultured in FCS demonstrated a higher number of lipid droplets compared to those cultured in SR and ECS (p < 0.05). Less accumulation of lipids was observed in embryos supplemented with SR. The lowest and highest numbers of lipid droplets in oocytes corresponded to the Bi and Bt strain, respectively. The lowest amount of lipid droplets in embryos was observed in Bi (p < 0.05). In conclusion, supplementation of the in vitro development culture medium (synthetic oviduct fluid) with a synthetic substitute serum produced similar results in terms of embryo development compared to those obtained with FCS, but a decreased degree of lipid droplet accumulation was observed in the in vitro‐cultured embryos.     

Yields of cultured Pacific oysters Crassostrea gigas Thunberg improved after one generation of selection

Parental families (G₀) in three lines of Pacific oysters were selected based on live weight and meat yields at harvest. The average live weight yield of progeny (G₁) from crossing G₀-selected lines in seven trials was 9.5% greater than that of nonselected control families and live weight yields were significantly greater (ANOVA, P<0.001) in four out of seven trials. The response to selection was greater if G₁ families were tested at the same site as their parents' selection site rather than at a different site, although this effect was only significant for G₁ families of cohort 5 (P<0.01) but not cohort 7 (P>0.05). A significant genotype×environment interaction affected yields in both cohort 5 and cohort 7 (ANOVA; P<0.001). In addition, correlation between the yields of the same families planted at both intertidal and subtidal sites was positive but weak (cohort 5, r=0.30; cohort 7, r=0.35), indicating that selection for high yield in one environment would likely result in a low correlated response in a different environment. Nonetheless, it was possible to identify six families in cohort 5 and four families in cohort 7 that were among the top 10 families at both sites. Further evaluation of families across a wider range of environments is needed to determine if it is possible to substantially improve yields by selecting “generalist” families that perform well along the whole Pacific coast, or whether it will be necessary to select lines that are suited to particular sites.