Using air pressure cells to evaluate the effect of soil environment on the transmission of soilborne viruses of wheat

ABSTRACT An air pressure cell, a laboratory tool that precisely controls soil matric potential, was utilized in a novel approach to investigate the epidemiology and management of soilborne disease. Matric potentials of -1, -5, -20, and -40 kPa were established in cores of field soil infested with Wheat soilborne mosaic virus (WSBMV) and its presumed vector Polymyxa graminis. Equilibrated soil cores were planted to wheat (Triticum aestivum), and after intervals of growth under controlled environment, virus transmission was assessed by serological detection of the virus in washed roots. Transmission occurred at all but the driest soil matric potential tested, -40 kPa, in which only pores with a diameter of 7.4 mum or less were water-filled, possibly obstructing movement of P. graminis zoospores. By starting plants at -40 kPa for 10.5 days and then watering them to conducive matric potential, we found that WSBMV transmission occurred between 12 to 24 h at 15 degrees C, and within 36 h at 20 degrees C. No significant transmission occurred within 96 h at 6.5 degrees C. In contrast, transmission of Wheat spindle streak mosaic virus (WSSMV) did not occur at 15 degrees C (the only transmission temperature tested), suggesting either that WSSMV is unable to establish infection at 15 degrees C or that a different vector is involved. The air pressure cell is a novel tool with many potential applications in research on the epidemiology and management of soilborne pathogens. Applications of the precise environmental control attained through the use of air pressure cells range from assessing the effects of cultural practices on soilborne inoculum to standardized virulence assays for soilborne pathogens to preliminary screens of host resistance and pesticide efficacy.     

Tricuspid valve stenosis caused by fibrous adhesions to an endocardial pacemaker lead in a dog

Acquired tricuspid valve stenosis (TVS) is a rare complication of endocardial lead placement in humans that has not been described in the veterinary literature. We describe a 6.5-year-old Border terrier that was presented with right-sided congestive heart failure 5.5 years after placement of a transvenous pacemaker. Severe TVS was confirmed by cardiac ultrasonography and appeared to be secondary to excessive endocardial pacemaker lead within the right atrium. Repositioning of the lead proved impossible and subsequent postmortem examination demonstrated fusion of the tricuspid valve (TV) leaflets around the endocardial lead with associated narrowing of the tricuspid valve orifice. In addition, the loop of the endocardial lead was anchored by thick fibrous tissue to the right atrial wall. This case report suggests that if sufficient endocardial lead is left inside the heart, in dogs undergoing transvenous pacemaker therapy, the redundant lead can become adherent to the tricuspid valve apparatus and cause valvular stenosis.     

Red cell distribution width values and red cell distribution width-to-platelet ratio in Thoroughbred foals in the first 24 hours of life

Objectives

To report red cell distribution width (RDW) values, to calculate RDW-to-platelet ratio (RPR), and to investigate a possible correlation of RDW and RPR index values in neonatal foals classified as healthy or at risk based on clinical information from a population of foals up to 24 hours of life.

Design

Retrospective study conducted from records and CBCs of foals born between June and November from 2018 to 2020 foaling seasons.

Setting

Breeding farm.

Animals

Three hundred and nine neonatal full-term Thoroughbred foals.

Interventions

None.

Measurements and Main Results

Foals were evaluated by a veterinarian within 15 minutes after birth, and a blood sample was collected within 24 hours of life. Based on clinical information, 88 of 309 foals (28.4%) were considered at risk of perinatal disease, and 201 were healthy. Mean gestational age for the foals was 346.3 ± 9.7 days. RDW values did not differ between groups. Gestational length demonstrated to have a negative correlation with RDW (r = –0.156, P = 0.005) and mean corpuscular volume (r = –0.135, P = 0.01), indicating a link of these variables to foal maturity. RPR index was higher for at-risk (0.073 ± 0.018) than for healthy foals (0.068 ± 0.014, P = 0.01).

Conclusion

RPR might be a promising early indicator of disease for the field triage of neonatal foals.     

Spatial Analysis of Maize Response to Nitrogen Fertilizer in Central New York

An increasing number of farmers are considering the use of site-specific nitrogen (N) applications to maize (Zea mays L.) as a way of maximizing yield potential while minimizing fertilizer cost. The objectives of this 3-years experiment were to evaluate the spatial structure of yield response to N fertilizer and investigate the potential for site-specific N management under maize production in New York. Four experimental N rates (50, 110, 160, or 220 kg ha¹), two tillage systems (chisel till and zone-till) and two crop rotations (maizemaize and maizesoybean (Glycine max L.)) were superimposed over a 12 ha field in central New York State with a complex of Honeoye-Lima, Kendaia, and Lima soils ranging from moderately well to poorly drained soils. Pre-sidedress soil nitrate tests (PSNT) showed significant spatial structure but did not conform to that for crop N response, indicating that N fertilizer recommendations based on PSNT results cannot be simply applied in a site-specific management approach. Optimal N rate varied from 110 kg ha ¹ for the dry years 1999 and 2000 to 220 kg ha¹ for 1998, with a warm wet spring. Tillage treatments were generally comparable in N response. Spatial yield response analysis showed limited field-scale regionalization of both yield and profit response to N, suggesting that site-specific application of nitrogen is impractical. The greatest source of variability in N requirements was observed with the annual effects of weather, and presents a greater potential for precise N application than site-specific application. Annual variations in optimum N rate were not related to annual yield differences and yield potential itself does not appear to be a good predictor of N needs.     

De novo crypt formation and juvenile polyposis on BMP inhibition in mouse intestine

Little is known about the signaling mechanisms that determine the highly regular patterning of the intestinal epithelium into crypts and villi. With the use of mouse models, we show that bone morphogenetic protein (BMP)-4 expression occurs exclusively in the intravillus mesenchyme. Villus epithelial cells respond to the BMP signal. Inhibition of BMP signaling by transgenic expression of noggin results in the formation of numerous ectopic crypt units perpendicular to the crypt-villus axis. These changes phenocopy the intestinal histopathology of patients with the cancer predisposition syndrome juvenile polyposis (JP), including the frequent occurrence of intraepithelial neoplasia. Many JP cases are known to harbor mutations in BMP pathway genes. These data indicate that intestinal BMP signaling represses de novo crypt formation and polyp growth.     

Cardiac arrhythmias associated with acute major haemorrhage during craniectomy in a dog

An estimated 43% of total blood volume was lost during craniectomy in a 32‐kg Labrador retriever. One episode of bradycardia, followed by ventricular tachycardia occurred during surgery when the rate of haemorrhage exceeded fluid replacement. Sinus rhythm was re‐established with intravenous lidocaine (1.25 mg kg^?1). Twenty‐four hours later, premature ventricular complexes appeared followed by episodes of ventricular tachycardia, some requiring lidocaine treatment. Myocardial hypoxia resulting either from hypovolaemia and/or air embolism (the right transverse venous sinus was damaged during surgery) may have caused the arrhythmias. Mild intracranial hypertension may have aggravated the problem.     

PIROPLASMOSIS IN BUFFALOES AND ITS SEROLOGICAL DIAGNOSIS BASED ON A HOMOLOGY BETWEEN BUFFALO AND BOVINE IMMUNOGLOBULINS

Babesia argentina, Babesia bigemina and Theileria mutans were transmitted experimentally from water buffaloes (Bubalus bubalis) to splenectomised Bos taurus calves. Buffaloes were positive to an indirect fluorescent antibody test for B. argentina when reagents of bovine origin were used. The formation of similar patterns during immunoelectrophoresis suggested a homology of buffalo and bovine serum proteins, particularly IgG.     

Energy intake and requirement of dairy cows during the whole year

It is argued that in view of the many new methods in dairy farming an economic model of the dairy farm should be available to improve advisory work. Some quantitative data concerning the dairy cow needed for such a model are given. They deal with a net energy feeding value of grass, hay, silage, artificially dried grass and concentrates estimated with a feed evaluation system based on results obtained with lactating cows. Also data on the requirements for net energy and for long forage dry matter are given, again obtained with such cows. Finally information is given on voluntary intake of forage dry matter at pasture, from fresh grass fed indoors, from various preserved forages including forage pellets, as influenced by milk yield and condition of the cow and by kind of forage and level of concentrates fed. Special attention has been paid to the precision of the information. These data are used to predict both the maximal and minimal amounts of long forage which can be fed in the stall period without loss of production, and the additional quantities of concentrates needed. For the grazing cow the problem of the adjustment of voluntary intake of grass to milk yield and amount of concentrates fed is studied. It is concluded that the information is sufficient to develop an economic model of a dairy farm. For its development grassland research should supply data on pasture yield in terms of digestible organic matter when the forage is fed to sheep at the maintenance level, and the economists should add data on costs of food and labour.     

Estrogenic potency of food-packaging-associated plasticizers and antioxidants as detected in ERalpha and ERbeta reporter gene cell lines

This study presents the estrogenic potency of 21 food-packaging-associated compounds determined for the first time, using two transfected U2-OS (human osteoblasts devoid of endogenous estrogen receptors) estrogen receptor (ER) alpha and beta cell lines. Six plasticizers and three antioxidants were slightly estrogenic in the ERalpha cells. The model compounds bisphenol A and nonylphenol, one plasticizer [tris(2-ethylhexyl)trimellitate (TEHTM)], and two antioxidants (propyl gallate and butylated hydroxyanisole) were estrogenic in both ERalpha and ERbeta cells. Compared to estradiol (E2), these compounds appeared to be relatively more estrogenic in the ERbeta cells than in the ERalpha cells. Three sorbitol-based plasticizers activated neither ERalpha nor ERbeta and may be good replacements of existing plasticizers. All responses were additive with the response of E2. This indicates that they may contribute to the total effects of the pool of estrogenic compounds humans are exposed to. The estrogenic potencies of these compounds, together with the suggested beneficial effect of ERbeta-mediated responses and adverse ERalpha-mediated effects, support the importance of detecting characteristics for ERalpha and ERbeta response separately in independent models, as done in the present study.