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481.
Nitish Kunte Erin McGraw Sydney Bell David Held Luz‐Adriana Avila 《Pest management science》2020,76(1):26-41
RNA interference is a phenomenon in which the introduction of double‐stranded RNA (dsRNA) into cells triggers the degradation of the complementary messenger RNA in a sequence‐specific manner. Suppressing expression of vital genes could lead to insect death, therefore this technology has been considered as a potential strategy for insect pest control. There are three main routes of dsRNA administration into insects: (i) injections to the hemolymph, (ii) topical, and (iii) feeding. In this review, we focus on dsRNA administration through feeding. We summarize novel strategies that have been developed to improve the efficacy of this method, such as the use of nano‐based formulations, engineered microorganisms, and transgenic plants. We also expose the hurdles that have to be overcome in order to use this technique as a reliable pest management method. © 2019 Society of Chemical Industry 相似文献
482.
James M. Forwood Erin J. Bubner Matt Landos Trent D’Antignana Marty R. Deveney 《Fish physiology and biochemistry》2016,42(1):103-109
Regulatory approval is being sought to use praziquantel (PZQ) to treat flukes infecting yellowtail kingfish (YTK), but accurate safety data were not available. We investigated the effect of increased doses or prolonged exposure of orally administered PZQ on YTK by assessing changes in haematological and biochemical characteristics, and mortality. Fish were intubated daily for 3 days with 0, 100, 300 and 500 mg PZQ kg?1 BW day?1 or once daily for 9 days at 0 and 100 mg PZQ kg?1 BW day?1. Blood was taken 24 h after the cessation of treatment. There was no significant difference between any of the haematological or biochemical indices in YTK treated with PZQ and controls, indicating that PZQ is safe for use at 100 mg PZQ kg?1 BW day?1 in YTK and that exposure to high doses or prolonged duration does not have negative effects on the YTK haematological or biochemical parameters we measured. 相似文献