全文获取类型
收费全文 | 1948篇 |
免费 | 129篇 |
国内免费 | 2篇 |
专业分类
林业 | 166篇 |
农学 | 36篇 |
基础科学 | 12篇 |
358篇 | |
综合类 | 328篇 |
农作物 | 60篇 |
水产渔业 | 146篇 |
畜牧兽医 | 781篇 |
园艺 | 63篇 |
植物保护 | 129篇 |
出版年
2024年 | 4篇 |
2023年 | 17篇 |
2022年 | 28篇 |
2021年 | 54篇 |
2020年 | 56篇 |
2019年 | 78篇 |
2018年 | 66篇 |
2017年 | 59篇 |
2016年 | 62篇 |
2015年 | 48篇 |
2014年 | 63篇 |
2013年 | 84篇 |
2012年 | 150篇 |
2011年 | 179篇 |
2010年 | 85篇 |
2009年 | 60篇 |
2008年 | 141篇 |
2007年 | 110篇 |
2006年 | 146篇 |
2005年 | 117篇 |
2004年 | 102篇 |
2003年 | 113篇 |
2002年 | 91篇 |
2001年 | 11篇 |
2000年 | 18篇 |
1999年 | 12篇 |
1998年 | 15篇 |
1997年 | 12篇 |
1996年 | 4篇 |
1995年 | 14篇 |
1994年 | 9篇 |
1993年 | 4篇 |
1992年 | 4篇 |
1991年 | 2篇 |
1990年 | 4篇 |
1987年 | 5篇 |
1986年 | 2篇 |
1985年 | 2篇 |
1984年 | 2篇 |
1983年 | 2篇 |
1980年 | 5篇 |
1979年 | 5篇 |
1978年 | 4篇 |
1977年 | 3篇 |
1975年 | 4篇 |
1974年 | 4篇 |
1973年 | 2篇 |
1971年 | 2篇 |
1957年 | 2篇 |
1954年 | 2篇 |
排序方式: 共有2079条查询结果,搜索用时 15 毫秒
61.
Objectives
To report our experience with thoracoscopic lung lobectomy in dogs with primary lung tumors and compare the long‐term outcome between dogs with primary lung tumors treated with lobectomy via thoracoscopy or thoracotomy.Study Design
Retrospective case series; case–control study.Animals
Thirteen dogs with primary lung tumors treated with thoracoscopic lung lobectomy; case‐matched cohort of 9 dogs with primary lung tumors treated with lobectomy via thoracotomy.Methods
Medical records from dogs that underwent thoracoscopic lung lobectomy at a single institution were reviewed. Only cases with primary lung tumors were included. Surgical complications and conversion rate were reported. Cases without conversion and with clean margins on histologic examination were matched on prognostic criteria to cases with primary lung tumors treated with lobectomy via thoracotomy. Survival was compared between thoracoscopy and thoracotomy.Results
Thirteen dogs with primary lung tumors that measured 2.2–7 cm underwent thoracoscopic lung lobectomy. Nine dogs were diagnosed with carcinoma and 4 with histiocytic sarcoma. There were 3 conversions to open thoracotomy because of poor visualization. There were no differences in short‐term outcome between dogs that had their procedures completed via thoracoscopy and those that were converted to thoracotomy. Nine dogs with clean margins were matched with 9 dogs treated via thoracotomy. Mean (standard deviation) followup was 367 (327) days for dogs undergoing thoracoscopy and 603 (612) days for dogs undergoing thoracotomy. The 2‐year survival rate was 44% for thoracoscopy and 56% for thoracotomy (P = .942).Conclusion
Selected primary lung tumors in dogs can be safely resected with thoracoscopy and not affect long‐term outcome.62.
Epigenetics provides a molecular mechanism of inheritance that is not solely dependent on DNA sequence and that can account for non-Mendelian inheritance patterns. Epigenetic changes underlie many normal developmental processes, and can lead to disease development as wel. While epigenetic effects have been studied in wel-characterized rodent models, less research has been done using agricultural y important domestic animal species. This review wil present the results of current epigenetic research using farm animal models(cattle, pigs, sheep and chickens). Much of the work has focused on the epigenetic effects that environmental exposures to toxicants, nutrients and infectious agents has on either the exposed animals themselves or on their direct offspring. Only one porcine study examined epigenetic transgenerational effects; namely the effect diet micronutrients fed to male pigs has on liver DNA methylation and muscle mass in grand-offspring(F2 generation). Healthy viable offspring are very important in the farm and husbandry industry and epigenetic differences can be associated with production traits. Therefore further epigenetic research into domestic animal health and how exposure to toxicants or nutritional changes affects future generations is imperative. 相似文献
63.
64.
Sebti I Chollet E Degraeve P Noel C Peyrol E 《Journal of agricultural and food chemistry》2007,55(3):693-699
Several properties of chitosan films associated or not with hydroxypropylmethylcellulose polymer (HPMC) and HPMC films incorporating or not nisin and/or milk fat were studied. Nisin addition at a level of 250 microg mL-1 and likewise chitosan at 1% (w/v) concentration were efficient for total inhibiting Aspergillus niger and Kocuria rhizophila food deterioration microorganisms. HPMC and chitosan films were transparent, whereas nisin and/or fat incorporation induced a 2-fold lightness parameter increase and, consequently, involved more white films. Measurements of tensile strength, as well as ultimate elongation, showed that chitosan and HPMC initial films were elastic and flexible. High thermal treatments and additive incorporation induced less elastic and more plastic films. Water vapor transmission as far as total water desorption rates suggested that chitosan films were slightly sensitive to water. Water transfer was decreased by <60% as compared with other biopolymer films. Regarding its hydrophobic property, the capacity of fat to improve film water barrier was very limited. 相似文献
65.
Extraction and characterization of oil bodies from soy beans: a natural source of pre-emulsified soybean oil 总被引:1,自引:0,他引:1
Iwanaga D Gray DA Fisk ID Decker EA Weiss J McClements DJ 《Journal of agricultural and food chemistry》2007,55(21):8711-8716
Soybeans contain oil bodies that are coated by a layer of oleosin proteins. In nature, this protein coating protects the oil bodies from environmental stresses and may be utilized by food manufacturers for the same purpose. In this study, oil bodies were extracted from soybean using an aqueous extraction method that involved blending, dispersion (pH 8.6), filtration, and centrifugation steps. The influence of NaCl (0-250 mM), thermal processing (30-90 degrees C, 20 min) and pH (2-8) on the properties and stability of the oil bodies was analyzed using zeta-potential, particle size, and creaming stability measurements. The extracted oil bodies were relatively small ( d 32 approximately 250 nm), and their zeta-potential went from around +12 mV to -20 mV as the pH was increased from 2 to 8, with an isoelectric point around pH 4. The oil bodies were stable to aggregation and creaming at low (pH = 2) and high (pH >/= 6) pH values but were unstable at intermediate values (3 = pH = 5), which was attributed to their relatively low zeta-potential. The oil bodies were stable to aggregation and creaming at relatively low salt concentrations (NaCl = 25 mM, pH 7) but were unstable at higher values as a result of electrostatic screening effects. The oil bodies were stable to thermal processing from 30 to 90 degrees C (0 mM NaCl, pH 7), but there appeared to be a change in their interfacial properties (decrease in zeta-potential) at temperatures exceeding 60 degrees C. These results suggest that oil bodies extracted from soybeans have similar or improved stability compared to soybean oil emulsions produced from bulk ingredients and may provide a new way of creating functional soy products for the food industry. 相似文献
66.
67.
Mar Costa-Hurtado Claudio L Afonso Patti J Miller Erica Spackman Darrell R Kapczynski David E Swayne Eric Shepherd Diane Smith Aniko Zsak Mary Pantin-Jackwood 《Veterinary research》2014,45(1):1
Low pathogenicity avian influenza virus (LPAIV) and lentogenic Newcastle disease virus (lNDV) are commonly reported causes of respiratory disease in poultry worldwide with similar clinical and pathobiological presentation. Co-infections do occur but are not easily detected, and the impact of co-infections on pathobiology is unknown. In this study chickens and turkeys were infected with a lNDV vaccine strain (LaSota) and a H7N2 LPAIV (A/turkey/VA/SEP-67/2002) simultaneously or sequentially three days apart. No clinical signs were observed in chickens co-infected with the lNDV and LPAIV or in chickens infected with the viruses individually. However, the pattern of virus shed was different with co-infected chickens, which excreted lower titers of lNDV and LPAIV at 2 and 3 days post inoculation (dpi) and higher titers at subsequent time points. All turkeys inoculated with the LPAIV, whether or not they were exposed to lNDV, presented mild clinical signs. Co-infection effects were more pronounced in turkeys than in chickens with reduction in the number of birds shedding virus and in virus titers, especially when LPAIV was followed by lNDV. In conclusion, co-infection of chickens or turkeys with lNDV and LPAIV affected the replication dynamics of these viruses but did not affect clinical signs. The effect on virus replication was different depending on the species and on the time of infection. These results suggest that infection with a heterologous virus may result in temporary competition for cell receptors or competent cells for replication, most likely interferon-mediated, which decreases with time. 相似文献
68.
Eric Saillant Liang Ma Xiaoxue Wang Delbert M Gatlin III & John R Gold 《Aquaculture Research》2007,38(8):781-788
Heritability of juvenile growth rate was estimated for red drum (Sciaenops ocellatus), an economically important sciaenid fish in the southern USA. Thirty‐eight families were generated via ‘natural’ spawning of multiple sets of five breeders (three dams × two sires) in individual brood tanks. Offspring were individually tagged with Passive Integrated Transponder (PIT) tags and mixed for grow‐out in replicate ‘common‐garden’ tanks. Juvenile growth was followed from 166.4±18.6 to 254.0±27.0 mm (total length). Offspring were assigned a posteriori to individual brood fish (dam and sire) based on genotypes at nuclear‐encoded microsatellites. Heritability (h2) of a thermal growth coefficient was estimated using an animal‐additive model and a restricted maximum‐likelihood algorithm. Estimates of h2 were 0.33±0.08 and 0.31±0.08 for thermal growth coefficient based on length and weight respectively. These results indicate a significant genetic component in juvenile growth rate in red drum. Estimates of h2 for condition coefficient (K) at various measurement dates averaged 0.38, suggesting a genetic component to shape in juvenile red drum. 相似文献
69.
Stefano Peruzzi Batrice Chatain Eric Saillant Pierrick Haffray Bruno Menu Jean-Claude Falguire 《Aquaculture (Amsterdam, Netherlands)》2004,230(1-4):41-64
Meiotic gynogenetic and triploid sea bass were produced by pressure shocks according to a previously published protocol. Pressure-treated groups did not survive as well as controls during early development and larval rearing. Performances, sexual maturation and carcass quality were examined over a period of 34–45 months. At the age of 34 months, growth of the gynogenetic fish was comparable to that of the control but inferior in the triploid fish. A predominance of male fish was found within the triploid groups, while diploid and meiotic gynogenetic fish showed equal proportions of the sexes. Gonadal maturation in triploid fish was significantly impaired, particularly in the females that showed rudimentary ovaries. Triploid males exhibited primary maturation but proved to be gametically sterile. Pressure-induced triploids did not grow as well as diploids, but these results might be ascribed to specific on-growing conditions (communal rearing). The performance of gynogenetic sea bass was comparable to that of control. The superiority of diploid fish over their triploid counterparts was confirmed during the final growing period and more clearly so in females. Performances of triploids varied according to their maternal origin. Overall, striking qualitative differences between diploid and triploid fish were found at the age of 34 and 45 months, although the results varied in a gender-specific manner. A strong maternal effect was also observed. The potential advantages of triploid sea bass for aquaculture purposes are discussed. 相似文献
70.
Nicole L. Vollmer Patricia E. Rosel Keith D. Mullin Lori H. Schwacke Lance P. Garrison Brian C. Balmer Kevin Barry Anthony Martinez Brian M. Quigley Carrie Sinclair Todd R. Speakman Jesse Wicker Lynsey Wilcox Eric S. Zolman 《水产资源保护:海洋与淡水生态系统》2021,31(10):2951-2966
- Population structure of highly mobile marine organisms can be complex and difficult to study, but it is important to understand how populations partition themselves within their environment for accurate assessment of both natural and anthropogenic impacts and successful management. The 2010 Deepwater Horizon oil spill negatively impacted common bottlenose dolphins (Tursiops truncatus) within Mississippi Sound and the surrounding north central Gulf of Mexico (GOMx); however, little was known about their underlying population structure in these waters. Thus, it was unclear how many demographically independent populations were affected by the spill.
- Common bottlenose dolphin samples were collected throughout inshore waters of Mississippi Sound and coastal waters of the north-central GOMx. Mitochondrial DNA control region sequence data and 19 nuclear microsatellite loci were analysed to determine how many populations are present and characterize their range throughout these waters.
- Bayesian clustering and migration analyses identified two genetically distinct and demographically independent populations: one predominantly inhabiting Mississippi Sound and adjacent coastal waters, and a second population extending generally from offshore of Mobile Bay, Alabama, east along the Florida Panhandle. Neither of these populations align with the currently delineated management stocks previously used to estimate impacts from the oil spill on common bottlenose dolphins in this portion of the GOMx.
- These results suggest that revisions may be necessary so that management stocks accurately represent the demographically independent populations present in these waters. Furthermore, better comprehension of underlying population structure will enhance impact assessments on common bottlenose dolphins and provide more appropriate baseline data to support future restoration and conservation objectives.