A possible relativistic jetted outburst from a massive black hole fed by a tidally disrupted star

Gas accretion onto some massive black holes (MBHs) at the centers of galaxies actively powers luminous emission, but most MBHs are considered dormant. Occasionally, a star passing too near an MBH is torn apart by gravitational forces, leading to a bright tidal disruption flare (TDF). Although the high-energy transient Sw 1644+57 initially displayed none of the theoretically anticipated (nor previously observed) TDF characteristics, we show that observations suggest a sudden accretion event onto a central MBH of mass about 10(6) to 10(7) solar masses. There is evidence for a mildly relativistic outflow, jet collimation, and a spectrum characterized by synchrotron and inverse Compton processes; this leads to a natural analogy of Sw 1644+57 to a temporary smaller-scale blazar.     

Field inoculation effectiveness of native and exotic arbuscular mycorrhizal fungi in a Mediterranean agricultural soil

In sustainable agriculture, arbuscular mycorrhizal (AM) fungal inoculation in agronomical management might be very important, especially when the efficiency of native inocula is poor. Here, we assessed the effect of native and exotic selected AM fungal inocula on plant growth and nutrient uptake in a low input Trifolium alexandrinum-Zea mays crop rotation. We evaluated the effects of four exotic AM fungal isolates on T. alexandrinum physiological traits in greenhouse. Then, the field performances of T. alexandrinum inoculated with the exotic AMF, both single and mixed, were compared to those obtained with a native inoculum, using a multivariate analysis approach. Finally, we tested the residual effect of AM fungal field inoculation on maize as following crop. Multivariate analysis showed that the field AM fungal inoculation increased T. alexandrinum and Z. mays productivity and quality and that the native inoculum was as effective as, or more effective than, exotic AM fungal isolates. Moreover, the beneficial effects of AMF were persistent until the second year after inoculation. The use of native AMF, produced on farm with mycotrophic plants species, may represent a convenient alternative to commercial AM fungal inocula, and may offer economically and ecologically important advantages in sustainable or organic cropping systems.     

Food deprivation stimulates the luteolytic capacity in the gilt

The aims of this study were to study the effects of fasting on progesterone (P4) production in the pig and to verify whether fasting influences luteal expression of PGF(2alpha) receptor (FPr) and prostaglandin secretion. Superovulated prepubertal gilts were used; half of them were fasted for 72h starting on day 2 (F2) or 9 (F9) of the induced estrous cycle, respectively, while two groups (C2 and C9) served as respective controls. Plasma P4 and PGFM concentrations were determined by RIA while FPr mRNA expression in CLs collected at the end of fasting period was measured by real-time PCR. In experiment 1, plasma P4 concentrations in fasted gilts were significantly (P<0.01) higher than in controls starting from day 3 (F2; n=6) and 10 (F9; n=6). FPr mRNA expression was similar in F2 and C2 (n=6) CLs while it was significantly (P<0.05) higher in F9 than in C9 (n=6) CLs. In experiment 2, cloprostenol administered on day 12 significantly (P<0.05) increased FPr mRNA expression in CLs from both F9 (n=6) and C9 (n=6) gilts. At the time of cloprostenol injection PGFM levels were significantly higher (P<0.05) in the fasted group and cloprostenol-induced luteolysis in fasted but not in normally fed gilts. Results from this study indicate that fasting in prepubertal gilts induced to ovulate stimulates luteal P4 and PGFM production as well as FPr mRNA expression, thus increasing luteolytic susceptibility.     

Gene expression induced by chronic ozone in the Mediterranean shrub Phillyrea latifolia: analysis by cDNA-AFLP

Seedlings of Phillyrea latifolia L., a Mediterranean shrub, were exposed for 90 days to 110 nl l(-1) ozone (O(3)). Comparison of the cDNA-amplified fragment length polymorphism (cDNA-AFLP) patterns for treated and control plants allowed the identification and cloning of 88 differential sequences induced by O(3). The differential expression of 67 cloned sequences was further confirmed by RT-PCR. The functions of 36 cloned sequences, corresponding to seven of the twelve gene functional classes of Arabidopsis, were presumed on the basis of their homology with characterized gene sequences. Ozone induction of genes homologous to 24 of the clones has been reported in other plant species, whereas the induction of the 12 remaining sequences has not been observed before. Ozone activation of these newly identified genes could be a result of the chronic exposure to low O(3) concentration, because in most previous studies, acute treatments, involving high O(3) dosages, were applied. Possible roles of the cloned sequences in the response of P. latifolia to O(3) and other causes of oxidative stress are discussed.     

Development and application of solvent-free extraction for the detection of aflatoxin M1 in dairy products by enzyme immunoassay

The official methods for the quantification of aflatoxin M1 in dairy products (cheese and yogurt) include extraction into dichloromethane or chloroform, evaporation of the solvent, partitioning of the reconstituted residue with hexane, and subsequent analysis. To secure a rapid and inexpensive screen for aflatoxin M1 contamination, a sensitive competitive ELISA, using a rabbit polyclonal antibody, was developed for measuring aflatoxin M1 in milk and used in a comparative study for measuring the extraction efficiency of aflatoxin M1 in aqueous or organic solvent buffers using yogurt samples. An aqueous sodium citrate solution was found to be suitable for extracting aflatoxin M1, thus eliminating the need for organic solvents. The citrate extraction proved to be efficient (recovery ranged from 70 to 124%) in fortified samples of very different kinds of dairy products, including yogurt and six types of cheese. Fourteen yogurt and cheese samples were extracted with citrate solution and analyzed by ELISA. A good correlation was observed (y=0.95x-0.59, r2=0.98) when the data were compared with those obtained through the official method, across a wide range of aflatoxin M1 contaminations (10-200 ng/kg).     

Gibberellic acid and flower bud development in loquat (Eriobotrya japonica Lindl.)

The application of gibberellic acid (GA₃) to the whole loquat tree from mid-May to early June and from early August to the onset of flowering, significantly reduced the number of premature flowering shoots per current shoot and per m³ of canopy, and so reduced the total number of panicles per m³ of canopy. The number of vegetative shoots per m³ of canopy was also significantly reduced by applying GA₃. The response depended on the concentration applied and produced optimal results at 50 mg l⁻¹. Differences in the number of flowers per panicle and leaves per shoot were not significantly modified by the treatment. Nevertheless, GA₃ applied directly to the developing apex near to flower differentiation reduced the number of flowers per panicle by 25–35% and without modifying the morphological characteristics of the panicle. Results suggest that less sprouting of lateral buds was largely responsible for the reduction in flowering intensity caused by GA₃. Best treatments reduced thinning costs (60%, approximately) of premature flowering shoots, slightly increased fruit diameter and significantly improved fruit colour and juice quality, thus advancing harvest date.     

Changes in some blood parameters,milk composition and yield of buffaloes (Bubalus bubalis) during the transition period

In this study changes in hematochemical parameters, milk composition and yield were investigated in buffaloes during the transition period. A total of 93 buffaloes 113.9 ± 8.03 months old and 535 ± 50 kg average body weight were used. Parity was recorded, blood samples were collected from 80 days pre‐partum until 70 days post‐partum; milk samples were collected from 5 days to 70 days post‐partum. On serum samples, the values of non‐esterified fatty acids, β‐hydroxybutyrate, glucose, cholesterol, triglycerides, aspartate aminotransferase, alanine aminotransferase, gamma‐glutamyltransferase, urea, total proteins were evaluated. On milk, percentage of fat, protein and lactose, along with the somatic cell count (SCC), milk yield and daily milk production (DMP) were assessed. The peripartum period significantly (P ≤ 0.01) influenced all studied parameters with the exception of glucose. Milk fat percentage showed decreasing trend from 10 until 40 days post‐partum; DMP significantly (P ≤ 0.01) increased from 1 day post‐partum until 40 days post‐partum. Milk yield significantly (P ≤ 0.01) decreased in animals over the sixth lactation. Our results confirmed the importance of transit period in buffaloes. Blood parameters and milk composition alterations are crucial to predict the energy balance status of buffaloes in order to improve their management and feed intake during the transition period.