Determination of phospholipids in olive oil by 31P NMR spectroscopy

A nondestructive analytical method based on NMR spectroscopy was developed for the determination of phospholipids in olive oil. The phospholipids extracted from virgin olive oil with a mixture of ethanol/water (2:1 v/v) were identified and quantified by high resolution (31)P NMR spectroscopy. The main phospholipids found in olive oil were phosphatidic acid, lyso-phosphatidic acid, and phosphatidylinositol. Validation of the (31)P NMR methodology for quantitative analysis of phospholipids in olive oil was performed. Sensitivity was satisfactory with detection limits of 0.25-1.24 mumol /mL. In addition, the composition of fatty acids in phospholipids model compounds and those in olive oil samples was estimated by employing one- and two-dimensional (1)H NMR. The results indicated that the fatty acid composition in phospholipids and triacylglycerols of olive oil was similar.     

Comparative Utilization of Different Fibre Feedstuffs by Weaning/Growing Pigs in the Tropics

This study was conducted to investigate the effect of feeding different fibre feedstuffs on the performance, nutrient utilization and economics of production of weaning/growing pigs. Five fibre feedstuffs--palm kernel cake （PKC）, wheat offal （WO）, corn bran （CB）, rice bran （RB） and brewers＇ dried grain （BDG） were used at 25% in each of the five experimental diets that were formulated in this study. Twenty weaner pigs （12.75 ± 0.6 kg） were used for the growth study while 15 pigs （14.95 ± 0.57 kg） were used for the digestibility study. The design of the experiment was completely randomized. The results of the study showed that the performance of the experimental animals were significantly influenced （P 〈 0.05） by the dietary treatments. The apparent digestibility of the crude protein, crude fiber and ether extract showed significant differences （P 〈 0.05） while there was no significant difference （P 〉 0.05） in the apparent digestibility of their dry matter, ash and nitrogen free extract. The diet that contained WO had the best growth response and apparent nutrient digestibility. Results of economics of production also showed that diet with WO gave the best （P 〈 0.05） value in feed cost/＇kg gain and net profit/animal. It can be concluded from this study that although all the animals fed the different fibre feedstuffs performed satisfactorily on all the parameters monitored, but for optimum growth, apparent nutrients digestibility and economics of production. WO should be used as a fibre feedstuff in the diets of weaning/growing pigs in the tropics.     

Anthocyanin and Oil Contents,Fatty Acids Profiles and Antioxidant Activity of Mexican Landrace Avocado Fruits

Mexican landrace avocados are naturally distributed mainly in high areas of central Mexico, where they have been produced and consumed since pre-Hispanic times. However, trees of these species are being replaced by improved varieties with greater global demand, and many species have been lost due to the destruction of their natural habitats. Many people in Mexico like to consume the pulp and peel of these fruits and have done so since pre-Hispanic times. This is because the peel of Mexican landrace avocados, unlike the peel of Hass avocados, is very thin and flavorful. The peel color may be bluish-purple or dark reddish due to the presence of anthocyanins, which are compounds with antioxidant activity. The objective of this study was to assess the oil and anthocyanin contents and to evaluate the antioxidant activity in fruits of 11 accessions collected from producing-regions of Mexico. The oil content was 16.2 to 32.3 g 100 g^?1 in pulp, and the main unsaturated fatty acids were oleic, linoleic and palmitoleic acids, depending on the accession. The anthocyanin contents in peels ranged from 0.64 to 47 mg g^?1 fresh weight. The highest antioxidant activity was found in the peel (53.3–307.3 mmol g^?1 fresh weight). The results confirm that the pulp and peel of dark-peel Mexican landrace avocados could be important nutraceuticals for humans.

     

In winter wheat (Triticum aestivum L.), post-anthesis nitrogen uptake and remobilisation to the grain correlates with agronomic traits and nitrogen physiological markers

In wheat, nitrogen (N) uptake and remobilisation after flowering contributes largely, in Northern countries, to grain yield and grain protein content. The aim of our study was first to estimate the proportion of N taken up and remobilised to the grain as well as their relative efficiency using ¹⁵NO₃⁻-labelling at flowering. The validity of the technique was assessed in comparison to the N budget calculation method on five winter wheat cultivars grown for 2 years at low and high fertilization input. We estimated that on average 71.2% of grain N originates from remobilisation with significant genotypic differences. Among the five genotypes, significant differences were also found for both N remobilisation efficiency (from 69.8 to 88.8%) and N translocation efficiency (from 89.7 to 93.4%). In parallel, during 1 year, we monitored physiological markers representative of N assimilation and recycling at two sampling dates during the grain filling period. We then examined if there was any relationship between these physiological markers, N absorption and remobilisation estimates and agronomic traits related to yield and grain N content. Nitrate reductase (NR) activity was highly correlated to N absorbed post-flowering and to grain protein content. Glutamine synthetase (GS) activity was even more highly correlated than NR activity to the amount of N remobilised and grain yield. The use of physiological traits such as NR and GS activities as markers of the wheat N status is discussed.     

Effect of cutting edge thickness and state of wear of ploughshare on draught force and heart rates of Sanga oxen in Ghana

In this study, draught forces and heart rates of oxen were measured during ploughing to assess ploughshare cutting edge performance. The main objective of the study was to examine the effect of cutting edge thickness and state of wear of animal-drawn mouldboard ploughshares on the draught force and the heart rates of oxen pulling the implement and to determine the optimum cutting edge that gives the least draught force and lowest heart rates when pulled by animals. To minimise soil variability, a field was ploughed, harrowed and left to consolidate for 4 weeks before field experiments started. A pair of Sanga oxen was used during the field test. Analyses of variance showed there were significant differences (p < 0.05) in the mean draught forces and the corresponding oxen heart rates for the cutting edges. The field test results indicated the draught forces and heart rates of the animals increased with increasing cutting edge thickness from 2 to 8 mm, but also increased slightly for the 1 mm edge. The draught forces of the other cutting edges relative to the 2 mm were 9%, 1%, 29%, 35% and 65% higher for the 1, 4, 8 mm, Reversed and double-sided, respectively. The heart rates induced by the other cutting edges relative to the 2 mm varied from under 1 to 12%. The draught of the cutting edges was between 6 and 10% of the body weight of the animals used for the experiment. The lowest draught force and heart rate were obtained for the 2 mm cutting edge. Since the 4 mm cutting edge generated draught forces and heart rates values as low as the 2 mm, it was considered potentially more durable under field conditions and could be the recommended cutting edge thickness for animal-drawn shares in similar soil conditions used for the experiment.     

Seed morphological characteristics,storage behavior,and germination pattern of Combretum indicum (L.) DeFilipps

Genetic Resources and Crop Evolution - Combretum indicum (L.) DeFilipps seeds have anthelmintic properties and are recognized to be medicinal; however, the array of their morphological characters,...     

Management of sea cucumber stocks: patterns of vulnerability and recovery of sea cucumber stocks impacted by fishing

Identifying rates of change in the abundance of sea cucumbers under differing management regimes is fundamental to estimating commercial yields, identifying ecological interactions and facilitating management. Here, we review the status of sea cucumber stocks from a range of Pacific Island countries (Samoa, Tonga, Palau, Fiji and Papua New Guinea), some of which have had a moratorium on exports for up to a decade. We use a time‐series approach to look at variation in sea cucumber presence, coverage and density from survey and re‐survey data. Results give an appreciation of variation between ‘high’ status (less impacted) and depleted stocks. Survey data show marked declines in coverage and abundance as a result of artisanal fishing activity, and although species groups were not lost at a country level, local extirpation and range restriction was noted. Resilience and ‘recovery’ following cessation of fishing varied greatly, both among locations and among the species targeted. Worryingly, even after extended periods of moratorium, the density of some species was markedly low. In many cases, the densities were too low for commercial fishing, and may be at a level where the effective population size is constrained due to ‘Allee’ affects. From these results, we suggest that management regimes presently employed are generally not well aligned with the level of response to fishing mortality that can be expected from sea cucumber stocks. New adaptive, precautionary approaches to management are suggested, which would allow more timely interventions to be made, while refined information on stock dynamics is sought.     

Effect of temperature increase on the embryonic development of Patagonian red octopus Enteroctopus megalocyathus in controlled culture

One of the major problems involved in the controlled cultivation of Patagonian red octopus (Enteroctopus megalocyathus) is its long embryonic period ranging between 150–176 days, after which the hatching of planktonic paralarvae is achieved. The effect of temperature on the incubation of E. megalocyathus eggs was studied with the aim of establishing if a temperature higher than 12°C is effective to accelerate the embryonic development without altering their morphological and physiological conditions. Fertilized eggs obtained under controlled conditions at 11°C ± 0.1 were randomly distributed in 12 water baths of 30 L at 4 temperatures: 12, 14, 15 and 16°C ± 0.1°C. The experiment lasted until egg hatching occurred.The embryo growth rate was accelerated at 15–16°C, so the time spent in embryonic development can be reduced in 15% when compared with embryo development obtained from eggs incubated at 12°C. The embryos showed no significant differences in the final survival and were morphometrically similar in all stages of development at all temperatures. The increase in temperature from 12 to 16°C, even if it allowed a better growth, had high metabolic costs for embryos of E. megalocyathus. The activities of lipases and proteases were affected by interaction between temperature and the embryo stage, with high lipase activity observed in embryos of stage XV incubated at high temperatures and the highest levels of trypsin and chymotrypsin in stage XX at 14°C. The results suggest that 15°C could be the limit temperature to increase growth.     

Effects of live feed containing Panagrellus redivivus and water depth on growth of Betta splendens larvae

Panagrellus redivivus is a nematode with a high protein content and low‐cost production. It is successfully used in the larviculture of shrimp, however, it has not yet been evaluated as feed for Betta splendens larvae. Thus, the objective of this study was to evaluate P. redivivus as feed for betta larvae maintained at different depths of water. The experiment was based on a completely randomized 3 × 2 factorial design, represented by two feed types and one alternating feeding regime plus two water depths (2.5 and 5.0 cm). The Artemia feed promoted the highest weight gain, followed by the alternating feeding regime. The single use of the nematode as live feed produced the poorest weight gain. The water depth and the feeding method influenced the specific growth rate (SGR). At a depth of 2.5 cm, the alternating feeding promoted similar SGR compared to fish fed with Artemia only. Furthermore, at the greater water depth an improvement in SGR was observed in fish fed Artemia only, but the similarity to alternating feeding regime remains. Thus, the inclusion of nematode is an appropriate and lower cost feed strategy for the betta larviculture independent of water depths.