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排序方式: 共有426条查询结果,搜索用时 31 毫秒
91.
92.
Buescher JM Liebermeister W Jules M Uhr M Muntel J Botella E Hessling B Kleijn RJ Le Chat L Lecointe F Mäder U Nicolas P Piersma S Rügheimer F Becher D Bessieres P Bidnenko E Denham EL Dervyn E Devine KM Doherty G Drulhe S Felicori L Fogg MJ Goelzer A Hansen A Harwood CR Hecker M Hubner S Hultschig C Jarmer H Klipp E Leduc A Lewis P Molina F Noirot P Peres S Pigeonneau N Pohl S Rasmussen S Rinn B Schaffer M Schnidder J Schwikowski B Van Dijl JM Veiga P Walsh S Wilkinson AJ Stelling J Aymerich S 《Science (New York, N.Y.)》2012,335(6072):1099-1103
93.
P Jull E Browne BS Boufana S Schöniger E Davies 《Journal of Feline Medicine and Surgery》2012,14(9):646-649
Clinical summary: A 4-year-old Birman cat was presented with marked obtundation and non-ambulatory tetraparesis. Two well-demarcated, intra-axial T2-hyperintense, T1-hypointense structures, which did not contrast enhance, were evident on magnetic resonance imaging (MRI). Histopathology of the structures revealed metacestodes that were morphologically indicative of larval stages of Taenia species. Polymerase chain reaction amplification of a fragment within the 12S rRNA gene confirmed the subspecies as Taenia serialis. Practical significance: This is the first report of MRI findings of cerebral coenurosis caused by T serialis in a cat. Early MRI should be considered an important part of the diagnostic work-up for this rare clinical disease, as it will help guide subsequent treatment and may improve the prognosis. 相似文献
94.
Anja Kotzerke Sven Klemer Kristina Kleineidam Marcus A. Horn Harold L. Drake Michael Schloter Berndt-Michael Wilke 《Biology and Fertility of Soils》2010,46(4):415-418
The antibiotic sulfadiazine (SDZ) can affect denitrifying bacteria in soil. However, effects on denitrifiers in the gut of
earthworms have not been described so far. Therefore, the influence of SDZ-contaminated manure applied to soil on denitrifiers
in the gut of the earthworm Eisenia fetida was assessed by quantitative polymerase chain reaction targeting genes coding for nirK- and nirS-type nitrite reductases of denitrifiers. Gut contents of Eisenia fetida contained 2.5 × 106 and 5.1 × 105 gene copies of nirK and nirS, respectively, after 2 weeks in soils amended with manure only. Copy numbers of nirK and nirS in gut contents from manure treatments with SDZ were up to ten times less. Overall, the data indicate a negative impact of
SDZ on denitrifiers in the gut of earthworms. 相似文献
95.
Johanna Bartling Michael Schloter Berndt-Michael Wilke 《Biology and Fertility of Soils》2010,46(3):299-302
Ethyl tert-butyl ether (ETBE) and tert-amyl methyl ether (TAME) are synthetic compounds that are used as gasoline additives in order to reduce emissions from vehicles.
In the present study, effects of these compounds were investigated on the dynamics of inorganic N in soil. Therefore, soil
samples with different water contents were contaminated with ETBE and TAME, respectively. It could be shown that in the presence
of TAME and ETBE, NH4+–N accumulated; moreover, ETBE addition to soil resulted in increased NO2−–N concentrations. These results indicated for the first time that ETBE and TAME can affect nitrification in soil. Thus, their
impact on soil ecology should be investigated more intensively. 相似文献
96.
97.
Gordon WJ Conzemius MG Riedesel E Besancon MF Evans R Wilke V Ritter MJ 《Veterinary surgery : VS》2003,32(5):451-454
OBJECTIVE: To evaluate the relationship between limb function and radiographic evidence of stifle osteoarthrosis (OA) in dogs. STUDY DESIGN: The relationship between force platform data and radiographic OA score was evaluated on 2 separate days using regression analysis. Interday variation was also assessed. ANIMALS: Forty-one dogs with visible lameness and radiographic evidence of stifle OA. METHODS: Force platform data were collected at a velocity of 1.7 to 2.0 m/s for 5 trials on day 1 and day 8. Radiographs taken on day 1 were scored using a previously reported OA scoring system. RESULTS: No significant relationship was found between force platform data and OA score. No significant differences were found between any day 1 and day 8 force platform values. CONCLUSIONS: Although radiographic evidence of stifle OA provides evidence of pathology, it does a poor job of representing limb function. In addition, the absence of significant differences between day 1 and day 8 values in this population of dogs supports use of only a single force platform evaluation before measuring a treatment effect. CLINICAL RELEVANCE: The presence of OA in the stifle joint does not correlate with clinical function; radiographic outcome should be used cautiously as a predictor of clinical outcome. 相似文献
98.
Bovine viral diarrhoea virus (BVDV) is a pestivirus within the family Flaviviridae. In contrast to the members of the genus flavivirus, nothing is known about the viral entry route for pestiviruses. In this study, the process of BVDV infection following attachment to the cell surface was examined. BVDV clearly co‐localizes with clathrin, with early endosome antigen‐1 (EEA‐1), an early endosome marker, and also with lysosomal‐associated membrane protein‐2 (LAMP‐2), a lysosomal marker. BVDV internalization is inhibited by compounds that block clathrin‐ but not caveolae‐dependent endocytosis. These findings demonstrate that BVDV enters the cells via the clathrin‐coated pit pathway. 相似文献
99.
100.
Massey EJ Sundstedt A Day MJ Corfield G Anderton S Wraith DC 《Veterinary immunology and immunopathology》2002,87(3-4):357-372
Experimental autoimmune encephalomyelitis (EAE) is a T cell-mediated autoimmune disease commonly employed as a model for multiple sclerosis. Extensive studies have demonstrated that EAE may be prevented or ameliorated by the intranasal administration of soluble peptides representing encephalitogenic epitopes. There is increasing evidence that this peptide administration may function via the generation of regulatory cells. The mechanism of action of these cells remains controversial and it seems likely that it may vary between experimental models. At present the majority of work on regulatory cells has centred on characterising naturally occurring regulators, or those generated artificially ex vivo, and less is known about induced regulatory cells produced following peptide administration. This report aims to briefly outline the evidence for the existence of natural regulatory T cells and to introduce the sub-types of induced regulatory T cells now recognised.In several of these regulatory cell systems investigated to date, interleukin-10 (IL-10) has been shown to be important in cell function. This has not been directly investigated in a model employing peptide therapy to induce peripheral tolerance, hence the purpose of this study was to investigate the role of IL-10 in the generation of these regulatory cells. This work has employed both a TCR transgenic mouse system, for predominantly in vitro studies of cell function, and an IL-10 knock-out mouse strain to investigate in vivo disease protection. The results summarised in this report demonstrate that IL-10 is fundamentally important in the generation of disease protection following intranasal peptide therapy. 相似文献