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101.
Genome‐wide association analysis of salmon lice (Lepeophtheirus salmonis) resistance in a North American Atlantic salmon population 下载免费PDF全文
Christina M Rochus Melissa K Holborn Keng P Ang J A K Elliott Brian D Glebe Steven Leadbeater J J Tosh Elizabeth G Boulding 《Aquaculture Research》2018,49(3):1329-1338
Our objective was to detect single nucleotide polymorphisms (SNPs) associated with resistance to the salmon louse in the Saint John River aquacultural population of North American Atlantic salmon using estimated breeding values (EBVs) and 6K genotypes from the parent‐generation and lice count phenotypes from the challenged, but ungenotyped, offspring‐generation. In 2011 and 2012, we challenged recent smolts with approximately 100 copepodids each. Fish were euthanized once the lice reached the chalimus stages and lice count, sex, tank and salt water weight were recorded. We used a multiple trait model to estimate breeding values for the parent‐generation using their own fresh water weights and the salt water weights and lice counts of the offspring‐generation. Salmon lice count heritability for untransformed and transformed data was 0.17 and 0.29 respectively. Two different genome‐wide association study methods were compared: (i) forward multiple linear regression and (ii) a mixed linear model using principal components to correct for population stratification as implemented in the egscore function of GenABEL. The two methods detected different SNPs located on different chromosomes. The multiple regression method incorporated 70 SNPs found on chromosomes 2, 7, 9, 12, 14, 15, 21, 22, 1p/23, 24. Many SNPs entered into the forward multiple regression are likely to be false positives from not correcting for the observed population stratification and cryptic relatedness. In contrast, the mixed linear model identified only two SNPs, one on chromosome 1p/23 (6.9%) and one on chromosome 1q (6.1%) consistent with louse‐resistance being a quantitative trait. 相似文献
102.
Lia Siegelman‐Charbit J. Anthony Koslow Michael G. Jacox Elliott L. Hazen Steven J. Bograd Eric F. Miller 《Fisheries Oceanography》2018,27(5):475-488
The California Current System (CCS) is an eastern boundary current system with strong biological productivity largely due to seasonal wind‐driven upwelling and transport of the California Current (CC). Two independent, yet complementary time series, CalCOFI ichthyoplankton surveys and sampling of southern California power plant cooling‐water intakes, have indicated that an assemblage of predominantly cool‐water affinity fishes spanning nearshore to oceanic environments in the southern CCS has declined dramatically from the 1970s to the 2000s. We examined potential oceanographic drivers behind this decline both within and north of the CalCOFI survey area in order to capture upstream processes as well. Empirical orthogonal function (EOF) analyses using output from a data‐assimilative regional ocean model revealed significant relationships between the fish time series and spatial patterns of upwelling, upper ocean heat content and eddy kinetic energy in the CCS. Correlation and linear regression analyses indicated that the declining trend in fish abundance was correlated with a suite of factors: reduced offshore and increased inshore upwelling; a long term warming trend combined with more recent interannual variability in ocean temperature; weaker eddy kinetic energy north of Point Conception (35°N), potentially indicating reduced transport of the California Current (CC); increased influence of the California Undercurrent (CUC); and a decline in zooplankton displacement volume across the southern CCS. Understanding how changes in oceanography affect fish populations will offer insights into managing fisheries in a changing climate. 相似文献
103.
Effects of temperature on Renibacterium salmoninarum infection and transmission potential in Chinook salmon,Oncorhynchus tshawytscha (Walbaum) 下载免费PDF全文
M K Purcell C L McKibben S Pearman‐Gillman D G Elliott J R Winton 《Journal of fish diseases》2016,39(7):787-798
Renibacterium salmoninarum is a significant pathogen of salmonids and the causative agent of bacterial kidney disease (BKD). Water temperature affects the replication rate of pathogens and the function of the fish immune system to influence the progression of disease. In addition, rapid shifts in temperature may serve as stressors that reduce host resistance. This study evaluated the effect of shifts in water temperature on established R. salmoninarum infections. We challenged Chinook salmon with R. salmoninarum at 12 °C for 2 weeks and then divided the fish into three temperature groups (8, 12 and 15 °C). Fish in the 8 °C group had significantly higher R. salmoninarum‐specific mortality, kidney R. salmoninarum loads and bacterial shedding rates relative to the fish held at 12 or 15 °C. There was a trend towards suppressed bacterial load and shedding in the 15 °C group, but the results were not significant. Bacterial load was a significant predictor of shedding for the 8 and 12 °C groups but not for the 15 °C group. Overall, our results showed little effect of temperature stress on the progress of infection, but do support the conclusion that cooler water temperatures contribute to infection progression and increased transmission potential in Chinook salmon infected with R. salmoninarum. 相似文献
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107.
Malcolm R Brown Peter D Kube Richard S Taylor Nick G Elliott 《Aquaculture Research》2014,45(5):798-811
Rapid measurement of salmon flesh quality parameters (>400 samples day?1) was demonstrated in the laboratory and remotely at industrial sites. Visible‐near infrared spectroscopy (VNIRS) was applied to predict astaxanthin (AX) and fat content in farmed Atlantic salmon. Fish were sampled from thirteen batches (1–6 kg whole weight, containing 2.3–16.3% fat and 1.2–12.5 μg g?1 AX), and models validated on small (average ± SD: 1.4 ± 0.4 kg) and large fish (4.2 ± 0.9 kg). Both constituents were well predicted in minced Norwegian Quality Cutlet (NQC) samples (r2 ≥ 0.86; standard error of prediction (SEP) ≤0.7% for fat and ≤0.7 μg g?1 for AX). Comparable metrics were observed for AX prediction in whole NQCs (r2 = 0.80–0.88; SEP 0.7 μg g?1). Fat was better predicted in small fish than large fish for whole NQCs (r2 = 0.82, SEP 1.0% cf r2 = 0.59, SEP = 0.59%) and non‐destructive scanning through the skin of whole, gutted fish (r2 = 0.77, SEP = 1.2% cf r2 = 0.49, SEP = 1.5%). Models were also developed for screening polyunsaturated fatty acid (PUFA) concentrations, e.g. in NQCs for docosahexaenoic acid (r2 = 0.73) and n‐3:n‐6 PUFA (r2 = 0.89). 相似文献
108.
Stephanie M. Lalor David J. Connolly Jonathan Elliott Harriet M. Syme 《Journal of Veterinary Cardiology》2009
ObjectivesTo determine if natriuretic peptide concentrations are increased in cats with systemic hypertension and/or chronic kidney disease (CKD).Animals22 normal cats, 13 normotensive cats with mild-moderate CKD (NT-CKD), 15 hypertensive cats with mild-moderate CKD (HT-CKD) and 8 normotensive cats with severe CKD (NT-CKD-severe).MethodsN-terminal pro-B-type (NT-proBNP) and pro-A-type (NT-proANP) natriuretic peptides were measured in plasma samples from all cats using commercially available assays and concentrations in the normal and diseased groups compared using non-parametric statistical tests. Spearman's rank correlation was used to test for an association between natriuretic peptide and creatinine concentrations.ResultsNT-proANP was significantly higher in the NT-CKD-severe than the normal group of cats (P = 0.006) but there were no other differences between groups. NT-proBNP concentrations were significantly higher in the HT-CKD group than both the normal (P < 0.001) and the NT-CKD (P < 0.001) groups. NT-proBNP concentrations were also higher in the NT-CKD-severe (P < 0.001) and the NT-CKD (P = 0.005) groups than the normal group. NT-proANP but not NT-proBNP was significantly and positively associated with plasma creatinine concentration.ConclusionsMeasurement of NT-proBNP shows promise as a diagnostic marker for systemic hypertension in the cat. Its concentration is not significantly increased in cats with mild-moderate normotensive CKD. 相似文献
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Wellehan JF Green LG Duke DG Bootorabi S Heard DJ Klein PA Jacobson ER 《Comparative immunology, microbiology and infectious diseases》2009,32(5):379-394
Megachiropteran bats are biologically important both as endangered species and reservoirs for emerging human pathogens. Reliable detection of antibodies to specific pathogens in bats is thus epidemiologically critical. Eight variable flying foxes (Pteropus hypomelanus) were immunized with 2,4-dinitrophenylated bovine serum albumin (DNP-BSA). Each bat received monthly inoculations for 2 months. Affinity-purified IgG was used for production of polyclonal and monoclonal anti-variable flying fox IgG antibodies. ELISA and western blot analysis were used to monitor immune responses and for assessment of polyclonal and monoclonal antibody species cross-reactivity. Protein G, polyclonal antibodies, and monoclonal antibodies detected specific anti-DNP antibody responses in immunized variable flying foxes, with protein G being the most sensitive, followed by monoclonal antibodies and then polyclonal antibodies. While the polyclonal antibody was found to cross-react well against IgG of all bat species tested, some non-specific background was observed. The monoclonal antibody was found to cross-react well against IgG of six other species in the genus Pteropus and to cross-react less strongly against IgG from Eidolon helvum or Phyllostomus hastatus. Protein G distinguished best between vaccinated and unvaccinated bats, and these results validate the use of protein G for detection of bat IgG. Monoclonal antibodies developed in this study recognized immunoglobulins from other members of the genus Pteropus well, and may be useful in applications where specific detection of Pteropus IgG is needed. 相似文献