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21.
Bernardo Chessa Marco Pittau Maria Puricelli Rosanna Zobba Elisabetta Coradduzza Paola DallAra Sergio Rosati Giorgio Poli Alberto Alberti 《Research in veterinary science》2009,86(3):414-420
A DNA vaccine against contagious agalactia was developed for the first time, encoding the P48 of Mycoplasma agalactiae. Specific immune responses elicited in BALB/c mice were evaluated. Both total IgG and IgG1 were detected in mice vaccinated with pVAX1/P48. Proliferation of mononuclear cells of the spleen, levels of gamma interferon, interleukin-12, and interleukin-2 mRNAs were enhanced in immunized animals. Results indicate that pVAX1/P48 vaccination induced both Th1 and Th2 immune responses. Nucleic acid immunization could be a new strategy against M. agalactiae infections and may be potentially used to develop vaccines for other Mycoplasma diseases. 相似文献
22.
Vittorio Rossi Elisabetta Pattori Riccardo Bugiani 《European journal of plant pathology / European Foundation for Plant Pathology》2008,121(2):147-159
The dynamics of the production of Stemphylium vesicarium conidia and Pleospora allii ascospores from different inoculum sources on the ground were compared in a model system of a wildflower meadow mainly composed
of yellow foxtail, creeping cinquefoil and white clover. The meadow was either inoculated (each October) or not inoculated
with a virulent strain of S. vesicarium, and either covered or not covered with a litter of inoculated pear leaves. Spore traps positioned a few centimetres above
the ground were exposed for 170 7-day periods between October 2003 and December 2006. Ascospores and conidia were trapped
in 46 and 25% of samples, respectively. Ascospore numbers trapped from the pear leaf litter were about five times higher than
those from the meadow, while conidial numbers were similar from the different inoculum sources. The ascosporic season was
very long, with two main trapping periods: December–April, and August–October; the former was most important for the leaf
litter, the latter for the meadow. The conidial season lasted from April to November, with 92% of conidia caught between July
and September. The fungus persistently colonized the meadow: the meadow inoculated in early October 2003 produced spores until
autumn 2006. The present work demonstrates that orchard ground is an important source of inoculum for brown spot of pear.
Thus, it is important to reduce inoculum by managing the orchard ground all year long. 相似文献
23.
RETROSPECTIVE COMPARISON OF THREE‐DIMENSIONAL CONFORMAL RADIATION THERAPY VS. PREDNISOLONE ALONE IN 30 CASES OF CANINE INFRATENTORIAL BRAIN TUMORS
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Elisabetta Treggiari Thomas W. Maddox Rita Gonçalves Jerome Benoit Julia Buchholz Laura Blackwood 《Veterinary radiology & ultrasound》2017,58(1):106-116
Infratentorial tumors are relatively infrequent in dogs and a lack of data makes it difficult to offer prognostic information. Untreated, dogs with these neoplasms have shorter survival times than those with supratentorial tumors. The role of radiation therapy (RT) in the management of infratentorial tumors is poorly defined and tumoral/peritumoral swelling in this site is a potential cause of serious acute side effects. The aim of this retrospective, cohort study was to describe cases of infratentorial tumors treated with fractionated three‐dimensional conformal RT (3D CRT) and glucocorticoids (GC), and compare outcomes and survival with dogs affected by tumors in the same location that received GC alone. Thirty patients with a MRI diagnosis of infratentorial tumors were recruited (15 received RT and GC and 15 GC alone). None had mentation changes at presentation. For both groups, MRI and medical records were reviewed; and factors associated with survival were evaluated with Kaplan–Meier product limit survival and Cox regression analysis. Overall median survival time (MST) was 294 days (95% CI 42–545). The MST in the RT group was 756 days (95% CI 209–1302) vs. 89 days (95% CI 34.7–143.3 days) for those dogs treated palliatively with GC alone. This difference was statistically significant (P = 0.001). No other factors (including neurological signs, MRI features, tumor volume and total RT dose) were statistically associated with survival in the RT group. This study suggests that 3D CRT offers a survival advantage for dogs with infratentorial tumors compared to GC alone, and significant complications are uncommon. 相似文献
24.
Rossetti Antonio Mazzaglia Angelo Muganu Massimo Paolocci Marco Sguizzato Maddalena Esposito Elisabetta Cortesi Rita Balestra Giorgio Mariano 《植物病害和植物保护杂志》2017,124(6):563-575
Journal of Plant Diseases and Protection - Over the last decades, kiwifruit cultivation has gained increasing importance all over the world, but some bacterial diseases seriously threaten its... 相似文献
25.
Rifampicin treatment of canine pyoderma due to multidrug‐resistant meticillin‐resistant staphylococci: a retrospective study of 32 cases
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26.
Isidre Llorente Albert Vilardell Pere Vilardell Elisabetta Pattori Riccardo Bugiani Vittorio Rossi Emilio Montesinos 《European journal of plant pathology / European Foundation for Plant Pathology》2010,128(1):127-141
Stemphylium vesicarium, the causal agent of brown spot of pear, overwinters in the leaf residues of pear and herbaceous plants of the orchard floor.
Pseudothecia of the teleomorph, Pleospora allii, are formed on these residues where they produce ascospores. New methods were tested aimed at reducing this overwintering
inoculum and increasing the efficacy of control of brown spot of pear. Sanitation methods were evaluated in nine trials in
Girona (Spain) and Ferrara (Italy) over a 4-year period. The sanitation methods were leaf litter removal in December to February,
and application of biological control agents (commercial formulates of Trichoderma spp.) to the orchard ground cover from February to May. Fungicides were also applied to the trees during the pear-growing
season, scheduled according to the BSPcast model. The different methods were tested as stand-alone applications or in combination.
All methods consistently reduced the disease incidence at harvest on fruit with an efficacy between 30 to 60% for leaf litter
removal and more than 60% for the combination of leaf litter removal and biological control. Efficacy of sanitation alone
(leaf litter removal and biological control) in reducing the brown spot level on fruit was similar in most of the trials to
the efficacy obtained when fungicides were applied alone. However, integration of sanitation methods and fungicides did not
improve the efficacy of disease control over the level provided by fungicides alone. 相似文献
27.
Tito Caffi Sara Elisabetta Legler Riccardo Bugiani Vittorio Rossi 《European journal of plant pathology / European Foundation for Plant Pathology》2013,135(4):817-829
Chasmothecia of Erysiphe necator form in one season, survive winter and discharge ascospores that cause primary infections and trigger powdery mildew epidemics in the next season. A strategy for powdery mildew control was developed based on (i) the reduction in overwintering chasmothecia and on (ii) spring fungicide applications to control ascosporic infections timed based on estimate risk (two to five sprays per season). Several fungicides, the hyperparasite Ampelomyces quisqualis, and a mineral oil product were first tested as separate applications in a greenhouse and in vineyards. In the greenhouse, A. quisqualis suppressed chasmothecia formation by 41 %; fungicides and mineral oil suppressed chasmothecia formation by 63 % and ascospore viability by 71 %. In vineyards, application of boscalid + kresoxim-methyl or meptyldinocap once after harvest, as well as application of A. quisqualis pre- and post-harvest, delayed disease onset and epidemic development in the following season by 1 to 3 weeks and lowered disease severity (up to the pea-sized berry stage) by 56 to 63 %. Risk-based applications of sulphur and of synthetic fungicides provided the same control as the grower spray program but required fewer applications (average reduction of 47 %). Sanitation strategies were then tested by combining products and application times (late-season, and/or pre-bud break, and/or spring). Adequate disease control with a reduced number of sprays was achieved with the following combination: two applications of A. quisqualis (pre- and post-harvest), one application of mineral oil before bud break, and model-based applications of sulphur fungicides between bud break and fruit set. 相似文献
28.
Tognolini M Giorgio C Hassan Mohamed I Barocelli E Calani L Reynaud E Dangles O Borges G Crozier A Brighenti F Del Rio D 《Journal of agricultural and food chemistry》2012,60(36):8877-8884
The Eph tyrosine kinase receptors and their ephrin ligands play a central role in human cancer as their deregulated expression induces tumorigenesis with aggressive phenotypes. To evaluate their potential contribution to EphA2-ephrinA1 modulation, several colonic catabolites of dietary (poly)phenolics, known to be generated in vivo, were screened using an ELISA-based binding assay. Some of the catabolites inhibited the binding in a dose-dependent manner (IC(50) values from 0.26 to 43 μM). Functional studies on prostate adenocarcinoma cells revealed that pyrogallol and protocatechuic acid specifically antagonized ephrinA1-Fc-induced EphA2 phosphorylation at concentrations that were not cytotoxic. The active concentrations of pyrogallol appear to be close to what can be reached in vivo under physiological conditions. Finally, because of the roles played by the Eph-ephrin system not only in cancer development but also in neurodegeneration and diabetes, pyrogallol and protocatechuic acid are candidates for more detailed functional studies to elucidate their role in these pathophysiological processes. 相似文献
29.
C. Eliana Gattullo Andreina Traversa Nicola Senesi Elisabetta Loffredo 《Water, air, and soil pollution》2012,223(9):6035-6044
The objective of this study was to assess the removal of the endocrine disruptor 4-nonylphenol (NP) at a concentration of 1?mg?L?1 by ryegrass and radish during germination and growth. The decontamination process was evaluated in water only or in water containing two organic fractions, a soil humic acid (HA) and a river natural organic matter (NOM) at concentrations of 10 and 200?mg?L?1. The addition of these fractions aimed to simulate the organic content of real aqueous systems. At the end of germination and growth, residual NP was measured by chromatographic analysis. Also, NP phytotoxicity was evaluated during germination. In germination experiments, NP in water only was not toxic for ryegrass and radish which removed, respectively, 37 and 51?% of the initial NP added. In water added with HA or NOM at both doses, in general, NP did not influence or inhibited seed germination. Both doses of HA in water promoted the removal of NP by germinating seeds, whereas NOM exerted differentiated results in the two species as a function of the dose applied. After 2?days of growth, in all treatments both species almost completely removed NP and accumulated a very little fraction of product. This study demonstrated that both ryegrass and radish possess a relevant capacity to remove the endocrine disruptor NP from water also in the presence of different organic fractions, thus suggesting their use in the decontamination of real aquatic systems. 相似文献
30.
Spada E Proverbio D Groppetti D Perego R Grieco V Ferro E 《Journal of the American Animal Hospital Association》2011,47(1):67-71
Canine leishmaniasis during pregnancy is rarely reported, even in countries where the infection in dogs is endemic. The authors report a case of a 4 yr old bitch with leishmaniasis treated with meglumine antimoniate during pregnancy. The pregnancy and delivery were normal and the bitch presented improvement of the infection during treatment. Three puppies died within 2 days of birth and tested negative via real-time PCR for L. infantum. The two surviving puppies were followed clinically, serologically, and by real-time PCR until 1 yr of age with no evidence of congenital leishmaniasis. L. infantum DNA was detected with real-time PCR analysis of uterine tissue from the bitch at the time of ovariohysterectomy. PCR analysis was performed after an ovariohysterectomy of the bitch that was performed two months after parturition. Meglumine antimoniate use in the pregnant bitch may have prevented vertical transmission of leishmaniasis. 相似文献