Screening for low grain cadmium phenotypes in sunflower, durum wheat and flax

Cadmium (Cd) level in nonoilseed sunflower (Helianthus annuus L.), flax (Linum usitatissimum L.), and durum wheat (Triticum turgidum L. var. durum) grown on uncontaminated, alkaline soils has exceeded limits established in Northern Europe. Separate field experiments were conducted to investigate variability of grain Cd levels among sunflower, durum wheat and flax germplasm, and to seek an efficient screening method for future breeding. There were large variations in leaf Cd concentration among 200 sunflower lines. These lines performed more consistently for Cd uptake at the R5 stage than at the V8 stage across 4 locations with markedly differing soils. Cd concentration in V8 leaves was not related to Cd in grain. The positive correlation between R5 leaf Cd and kernel Cd level was obtained from nonoilseed hybrid (Sigco 954) (R²; = 0.74**), and 200 lines (R² = 0.44**) tested over 4 locations in 2 field trials, respectively. This indicates that an efficient and low cost screening method can be developed for genotype selection, but plants must be grown to the R5 stage. A preliminary evaluation of 30 durum wheat and 74 flax lines indicated large variations in grain Cd level of durum wheat and flax. Grain Cd concentration ranged from 0.11 to 0.34 mg Cd kg^-1 DW for durum wheat, and 0.14 to 1.37 mg Cd kg^-1 DW for flax, respectively. This variability indicates that breeding for low grain Cd in durum wheat and flax should be feasible. This revised version was published online in July 2006 with corrections to the Cover Date.     

不同频率下的泥石流体特征数值计算方法研究——以大寨沟流域为例

在泥石流定量研究中,泥石流流量、流速、泥石流规模是泥石流体最重要的特征值.能比较准确的确定泥石流这些特征数值既是泥石流计算中的关键问题,也是泥石流防治工程设计研究中的重要因素.利用雨洪法,首先比较一种或两种水文学的计算方法,择优来计算暴雨与洪水特征数值,然后利用暴雨与洪水特征数值来推算泥石流体各种特征数值.比如用暴雨洪峰流量以及配方法来计算泥石流流量,利用洪水总量来推算泥石流规模.     

Kinetic enzymatic determination of glycerol in wine and beer using a sequential injection system with spectrophotometric detection

A sequential injection system for the automatic determination of glycerol in wine and beer was developed. The method is based on the rate of formation of NADH from the reaction of glycerol and NAD+ catalyzed by the enzyme glycerol dehydrogenase in solution. The determination of glycerol was performed between 0.3 and 3.0 mmol L(-1) (0.028 and 0.276 g L(-1)), and good repeatability was attained (rsd < 3.6%, n = 5) for all samples tested. The determination rate was 54 h(-1), the reagent consumption was only 0.75 micromol of NAD+ and 5.4 ng of enzyme per assay, and the waste production was 2.12 mL per assay. Results obtained for samples were in agreement with those obtained with the batch enzymatic method.     

Perspectives on soil fertility change: a case study from southern Ethiopia

This paper explores the complexities underlying the apparently simple question—Is soil fertility declining?—for Wolayta in southern Ethiopia, an area famed for its high population densities and extreme pressure on resources. Drawing on extensive field research in the area, the paper examines differing perceptions of soil fertility decline, and attempts to pick apart the range of evidence of changes in soil fertility over time from a variety of different sources, ranging from historical interviews with farmers to fertilizer trials carried out over many years. In contrast to the definitive statements so often made in development policy statements, the actual situation is much less clear. A detailed understanding of soil fertility dynamics is only reached by a farm and field level examination of soil nutrient balances. Differences according to agroecological zone and socio-economic group are evaluated. These results show that soil fertility is indeed declining in some places, for some people and over certain periods, but it is not universal. Indeed, soil fertility is apparently increasing at certain sites. The implications of such findings for development policy and practice are briefly explored. Copyright © 1999 John Wiley & Sons, Ltd.     

马铃薯的钾素需求及营养诊断

了解马铃薯的钾素营养特点是马铃薯养分管理的基础,综述了马铃薯钾素营养的研究进展,并提出了进一步研究的方向。尽管文献报道的马铃薯需钾量存在差异,但已有的研究结果表明,马铃薯是需钾量较大的作物,其对钾素的需求多于其他元素。而且与小麦等作物相比,马铃薯的钾素营养效率较低。已有的研究还证实土壤交换性钾含量与马铃薯产量的关系并不稳定,仅依靠土壤测试制定钾肥施用方案存在较大的误差,因而马铃薯钾素营养状况诊断并依此指导生育期钾肥施用就显得必要。业已发现叶柄钾浓度能很好的反映马铃薯植株的钾素营养状况,对土壤供钾高度敏感,与块茎产量存在很好的数量关系,因此,用于指导马铃薯施肥的叶柄的临界钾浓度被不同研究人员确立,但干重基础的叶柄钾浓度指标指导施肥实践的时效性较差,这意味着简便快速的诊断方法有待建立。     

基于DPSIR模型的黄土高原生态敏感性演变格局及驱动力分析

摸清生态系统敏感性时空演变格局，对其驱动因素及未来发展趋势进行探测是修复和提高生态系统稳定性的重要内容。黄土高原是世界上水土流失最严重和生态环境最脆弱的地区之一，探究该地区生态敏感性时空演变特征及驱动因素，科学划分生态敏感性治理分区对于促进国家生态文明建设具有重要意义。基于“驱动-压力-状态-影响-响应”（driving-pressure-state-impact-response，DPSIR）框架，耦合空间距离指数和全排列多边形图示指标法构建生态敏感性指标体系，使用地理信息演变图谱和地理探测器研究黄土高原2000—2020年5个时间点生态敏感性时空格局和驱动力，最终划分生态敏感性治理分区。结果表明：1）研究期内黄土高原生态敏感性表现为西北高、东南低的分布特征，时间上先增加后下降。2）研究期内黄土高原生态敏感性转移类型以波动稳定型为主，生态改善区面积占比最大（35.17%），变化0次和1次的面积占比62.06%，生态环境演变稳定向好。3）生态敏感性的各驱动因素中，NDVI和降水量以及两者与其他因子的交互作用对于黄土高原生态敏感性具有明显的驱动作用。4）2030年低度敏感将成为黄土高原的主要敏感类型；沙地和农灌区以及黄土高塬沟壑区的中西部是生态敏感性治理的重点地区。整体来看，黄土高原生态环境有所好转，生态治理措施取得了积极成效，该研究可为黄土高原生态治理及高质量发展提供参考。     

Effect of heating oxymyoglobin and metmyoglobin on the oxidation of muscle microsomes

Myoglobin (Mb) and its iron have been proposed to be major prooxidants in cooked meats. To understand the mechanisms and differentiate between the prooxidant and antioxidant potential of oxymyoglobin (OxyMb) and metmyoglobin (MetMb), their prooxidant activity, iron content, solubility, free radical scavenging activity, and iron binding capacity were determined as a function of thermal processing. The ability of native and heat denatured OxyMb and MetMb to promote the oxidation of muscle microsomes was different. MetMb promoted lipid oxidation in both its native and denatured states. Conversely, OxyMb became antioxidative when the protein was heated to temperatures >or=75 degrees C. The increased antioxidant activity of heat denatured OxyMb was likely due to a decrease in its prooxidative activity due to its loss of solubility. These data show that the impact on oxidative reactions of Mb is the result of the balance between its antioxidant and prooxidant activities.     

Sr胁迫对油菜幼苗抗氧化指标影响的研究

在石英砂和Hoagland营养液培养条件下,用浓度为0、10、20和40mmol/L的88Sr2+(88SrC l2)处理油菜幼苗(Brassica napus L.)(三叶期到六叶期),动态研究Sr胁迫对油菜幼苗抗氧化指标的影响,以及Sr2+积累量和富集能力的变化。结果表明,油菜幼苗对Sr2+表现出高富集能力,富集能力依次为:叶茎根,Sr2+浓度越高,富集能力越低。与对照相比,Sr2+胁迫下的油菜超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量随Sr2+浓度的增加及胁迫时间的延长呈先上升后下降趋势;过氧化物酶(POD)和过氧化氢酶(CAT)活性则随富集Sr2+能力的提高而降低。因此得出结论,油菜幼苗对Sr胁迫具有较高的耐受能力和富集能力;叶片是最主要的富集器官;富集能力与POD,CAT活性显著负相关。     

Isoenzyme and cotyledon protein variation for identification of black beans (Phaseolus vulgaris L.) with similar seed morphology

Summary Methods developed to identify genetically diverse varieties of beans (Phaseolus vulgaris L.) were applied to closely related lines that were difficult to distinguish on the basis of seed morphology. Seedling tissues and seeds of black beans, were examined electrophoretically for isoenzyme and cotyledon proteinn protein patterns. Seven enzymes, extracted from seeds or from seedling stem, root or leaf tissues, were compared for polymorphism. Peptidase, polyphenol oxidase, phosphoglucoisomerase and glutamate oxaloacetate transaminase patterns were the same for all lines. Some differences were observed for acid phosphatase, peroxidase and esterase patterns, but complete discrimination of the six selected lines was not possible on the basis of isoenzyme patterns alone. Using polyacrylamide gel electrophoresis (PAGE) of dissociated 0.1 M acetic acid soluble proteins at pH 3.1 (acid-PAGE), or sodium dodecyl sulphate PAGE (SDS-PAGE) of residual protein extracts, all but one pair of samples in each case could be distinguished from the other samples. Using both techniques all of the lines could be identified unequivocally.     

锦绣黄桃在安顺的引种表现及主要栽培技术

锦绣黄桃是上海市农业科学院选育的鲜食与加工兼用晚熟黄桃新品种,其果个大、外形美观、营养丰富。2016年安顺市农业科学院引种到安顺种植,经过连续几年观察,其表现出适应性强、丰产性好、品质优良。初步认为可在安顺地区推广种植。