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排序方式: 共有109条查询结果,搜索用时 15 毫秒
101.
Eleni Nasiou Ioannis O. Giannakou 《European journal of plant pathology / European Foundation for Plant Pathology》2017,147(2):415-430
The protein kinase HOG1 in high osmolarity glycerol pathway is one of mitogen-activated protein kinases and plays an important role in fungi cellular responses to external stress and stimuli. In this study, AlHOG1, a yeast HOG1 homolog, was isolated from tobacco pathogenic fungus Alternaria longipes and its disrupted mutants were generated using homologous recombination. Compared with the wild-type strain, the mutants grew slower under Minimal-Media, produced less conidia on Potato-Dextrose-Agar and showed a retardation phenotype in conidial germination rate. Colony color observation indicated that AlHOG1 most likely negatively regulated melanin production under nutrients-deficient or osmotic stress. The same action mode of AlHOG1 on hyphal growth in response to certain carbon or nitrogen sources was speculated. Additionally, the disrupted strains were sensitive to osmotic and oxidative stress, and showed increased resistance to dicarboximide, phenylpyrrole and carbendazim fungicides. Meanwhile, results from the comparative analysis between the AlHOG1- and the AlHK1 (encoding a group III histidine kinase)-disrupted mutants showed that AlHOG1 mediated-pathway was partly regulated by the upstream AlHK1 kinase for cellular resistance to salts osmotic stress and that AlHK1 was a primary regulator for cellular sugar and fungicides stress adaptation and likely modulated both AlHOG1-mediated pathway and other unknown mechanisms. Noteworthily, AlHOG1 but not AlHK1 was confirmed to be associated with high concentration glycerol adaptation, which is the first report to our knowledge. Overall, our results indicated that AlHOG1 was involved in multiple physiological processes in A. longipes, including mycelial and conidial development, melanin production, nutrients sensing and multi-stress responses. 相似文献
102.
Theodoros Koutroumanidis Eleni Zafeiriou Garyfallos Arabatzis 《Forest Policy and Economics》2009,11(1):56-64
The present paper studies the existence of asymmetry in the price transmission mechanism between the producer and the consumer prices in the sector of forest products. In particular, the research is focused οn the round wood of long length (> 2 m). For the study of the asymmetry, the Johansen cointegration analysis was used while at the same time two dynamic models were estimated: The Error Correction Model (ECM Model), and the LSE?Henry general to specific model (GETS model). With the assistance of the cointegration technique, we surveyed the existence of a long-run relationship between the producers and the consumers in the Greek round wood market, while the application of the Granger causality test has shown that the consumer price Granger causes the producer price whereas the reverse is not valid. Furthermore, the application of the GETS model confirmed the existence of asymmetry in the price transmission mechanism within the round wood market. Finally, the role of imports in the determination of the producer prices is vital and is confirmed by the findings of the cointegration technique and the Granger causality test. 相似文献
103.
ABSTRACT The potential of volatile substances emitted by 'Isabella' grapes (Vitis labrusca) to control gray mold (Botrytis cinerea) on 'Hayward' kiwifruit (Actinidia deliciosa) was studied. The closed Mariotte system was used as a bioassay method to analyze quantitatively the biological action of these volatiles on B. cinerea growth. In vivo experiments compared the effects of volatiles from 'Isabella' grapes versus volatiles from 'Roditis' grapes (V. vinifera) and a B. cinerea control on the growth and disease development of B. cinerea on kiwifruit. The effect of the volatiles on the growth of B. cinerea was tested at various temperatures and times of inoculation after the wounding of kiwifruit, as well as using various weights and developmental stages of the grapes. The 'Isabella' volatiles limited the incidence of infection by reducing both the inoculum density and the activity of the pathogen. The weight and developmental stage of the grapes were important in the degree of inhibitory action of the 'Isabella' volatiles. The inhibitory action was more pronounced at 21 degrees C irrespective of the inoculation time after wounding. The study shows the potential for successful biological control of B. cinerea on kiwifruit by volatiles from 'Isabella' grapes. 相似文献
104.
Essential oils (EOs) from Rosemary, Rosmarinus officinalis (Lamiaceae), Orange, Citrus sinensis (Rutaceae), Lavandula, Lavandula angustifolia (Lamiaceae), False Yellowhead, Dittrichia viscosa (Asteraceae) and their major components were evaluated against root knot nematode, Meloidogyne incognita and Meloidogynejavanica. Second stage juveniles' (J2) paralysis and egg hatch inhibition were studied, while pulverized plant parts were tested for nematodes biological cycle arrest. All EOs paralyzed J2 and M. incognita were found more sensitive than M javanica with the EC50/4 days calculated at 250, 3,650 and 4,260 12g/mL for 1). viscosa, L. angustifolia and R. officinalis, respectively. Similarly, all EOs inhibited nematodes egg hatch and D. viscosa exhibited the highest inhibition on egg hatch (100% inhibition at 5 μg/mL). A significant influence of constituent terpenes (limonene, 1,8-cineole, linalool, camphor, L-borneol, caryophyllene oxide, β-eudesmol) dose and exposure time was indicated on egg hatch inhibition (56% to 100% at 500 μg/mL and 1,000 μg/mL), while only β-eudesmol achieved paralysis of J2 and specifically against M. incognita (EC50/1d = 50μg/mL). Interestingly, the most active botanical species arresting Meloidogyne spp. biological cycle in soil was C. sinensis (EC50 = 2 mg/g) and the most sensitive nematode species was M. javanica. The larvicidal and egg hatch inhibition activity holds promise towards the optimization of artificial terpene mixtures as novel and effective natural nematicides. Complex interactions of primary compounds and subsequent decomposition derivates compose efficacy profile of soil amendments. 相似文献
105.
Maria Kalemkeridou Ioannis Nanas Katerina Moutou Georgios S. Amiridis Andreas Tsipourlianos Eleni Dovolou Zissis Mamuris Themistoklis Giannoulis 《Reproduction in domestic animals》2023,58(1):146-157
Heat stress causes extensive losses in the dairy sector, due to negative effects on milk production and reproduction. Cows have evolved a series of protective mechanisms, (physiological, biochemical, behavioural) to cope with the thermostressing environments, which have allowed the preservation of productive and reproductive potential of specific animals during summer; these animals are considered thermotolerant and could be used to design programs of selective breeding. These programs, targeting the generations of a population of heat-resistant animals, would increase the frequency of the desired phenotypes, tackling the financial losses on one hand and reducing the carbon footprints of the dairy sector on the other. The development of genomics techniques has enabled genome wide variant calling, to detect SNPs associated with the desired phenotypes. In this study, we used a comparative genomics approach to detect genetic variation associated with thermotolerance and to design molecular markers for characterizing the animals as tolerant/sensitive. A total of 40 cows from each group were split in four sequencing pools and a whole-genome sequencing approach was used. Results and conclusion: Genome-wide genetic variation between groups was characterized and enrichment analysis revealed specific pathways which participate in the adaptive mechanisms of thermotolerance, implicated into systemic and cellular responses, including the immune system functionality, Heat Stress and Unfolded Protein Response. The markers made a promising set of results, as specific SNPs in five genes encoding for Heat Shock Proteins were significantly associated with thermotolerance. 相似文献
106.
Tzakos AG Kontogianni VG Tsoumani M Kyriakou E Hwa J Rodrigues FA Tselepis AD 《Journal of agricultural and food chemistry》2012,60(28):6977-6983
Betulinic acid, a natural pentacyclic triterpene acid, presents a diverse mode of biological actions including antiretroviral, antibacterial, antimalarial, and anti-inflammatory activities. The potency of betulinic acid as an inhibitor of human platelet activation was evaluated, and its antiplatelet profile against in vitro platelet aggregation, induced by several platelet agonists (adenosine diphosphate, thrombin receptor activator peptide-14, and arachidonic acid), was explored. Flow cytometric analysis was performed to examine the effect of betulinic acid on P-selectin membrane expression and PAC-1 binding to activated platelets. Betulinic acid potently inhibits platelet aggregation and also reduced PAC-1 binding and the membrane expression of P-selectin. Principal component analysis was used to screen, on the chemical property space, for potential common pharmacophores of betulinic acid with approved antithrombotic drugs. A common pharmacophore was defined between the NMR-derived structure of betulinic acid and prostacyclin agonists (PGI2), and the importance of its carboxylate group in its antiplatelet activity was determined. The present results indicate that betulinic acid has potential use as an antithrombotic compound and suggest that the mechanism underlying the antiplatelet effects of betulinic acid is similar to that of the PGI2 receptor agonists, a hypothesis that deserves further investigation. 相似文献
107.
108.
Eleni Papantoniou Eugene W. Hammond Fiona Scriven Michael H. Gordon J. David Schofield 《Cereal Chemistry》2001,78(6):663-665
A preparative high‐performance liquid chromatographic method was developed for the isolation of polar lipid classes of mono‐ and digalactosyldiacylglycerols (MGDG and DGDG), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), and phosphatidylcholine (PC) from wheat flour lipid extracts in relatively large quantities. The method allowed the separation of ≤100 mg/injection of lipid mixture. MGDG, DGDG, and PC were isolated as pure lipid classes, whereas PE was isolated in a mixture with PG, PC, and lysophosphatidylcholine (LPC). The availability of this methodology will facilitate research aimed at investigating the functional role of such lipids in foods. 相似文献
109.
The physicochemical properties of small‐ and large‐granule wheat starches were investigated to reveal whether gelatinization properties and rheological behavior differ between size classes of wheat starch. All samples contained 60% water (w/w, wb). The starch granule size and shape were examined by scanning electron microscopy in the separated A‐ and B‐type granule populations and in the whole wheat starch granule population. Differential scanning calorimetry (DSC) and electron spin resonance (ESR) analyses were performed in parallel with rheological measurements using dynamic mechanical thermal analysis (DMTA) to relate the viscoelastic changes to modifications in dynamic properties of aqueous solutions and structural disorganization of starch. The small (B‐type) granules had slightly higher gelatinization temperature and lower gelatinization enthalpy than did the large (A‐type) granules. Also, B‐type granules had higher enthalpy for the amylose‐lipid complex transition. Moreover, our results suggested that small granules have higher affinity for water at room temperature. It seems that there is a less ordered arrangement of the polysaccharide chains in the smaller granules when compared with the larger ones. These differences in functional properties of small and large granules suggested that the granule size distribution is an important parameter in the baking process. 相似文献