Low temperature-induced sterility in rice: Evidence for the effects of temperature before panicle initiation

Spikelet sterility of rice (Oryza sativa L.) results from low temperatures during panicle development. However, this temperature alone cannot fully explain the fluctuations in sterility observed in the field, since the susceptibility of rice plants to low temperature often changes according to its physiological status during sensitive stages. In the present study, we examined whether temperatures before the panicle initiation stage (PI) influence the plant's susceptibility to sterility during panicle development. To test this, we conducted a 2-year pot study and an analysis of field data using a model of cooling degree-days (CDD). In the pot study, the air temperature (T_a) and water temperature (T_w) were controlled independently for 3 weeks during the vegetative growth stage before PI. After PI, the plants were submerged in a cool water bath at a critical temperature of 19.5 °C to induce floral sterility. We found that low T_w during vegetative growth significantly increased the sterility. Low T_a during vegetative growth also significantly increased the sterility, but this effect was diminished by warm T_w even at low T_a. There was a close and negative correlation between sterility and T_w during vegetative growth. In the analysis of field data, we introduced CDD using temperatures below a threshold level of 20 °C to represent the magnitude of the exposure to low temperature from PI to the heading stage. Data of T_a was used for this analysis because data of T_w was scare. The CDD model was applied to 77 independent data sets collected at nine Agricultural Research Centers during four typical cool summers (1980, 1988, 1993, and 2003) in northern Japan. Year-to-year variations in sterility at one site were roughly accounted for by the variations in CDD, but large deviations were observed among the years. The deviations were related to T_a averaged over the 30-day period before PI. For a similar level of CDD, the lower the T_a before PI, the greater the sterility. Similar deviations were observed in the between-site relationships between sterility and CDD, and these deviations were related with the T_a before PI. These results suggest that temperatures before PI, and especially T_w, change the susceptibility of a rice plant to low temperatures during panicle development.     

Impact of aquatic plants on entrainment phenomena based on wind-induced flow in a closed density stratified water area

It has recently been proposed that water purification could be performed using aquatic plants, since they absorb nutrient salts. The behavior of a substance in a closed water area is affected by turbulent flows from wind-induced flow, which is a mechanical disturbance, and convective flow, which is a thermal disturbance. In a closed density stratified water area, wind-induced flow gives rise to the entrainment phenomenon at the density interface. This phenomenon, which is based on mixing between the upper and lower water layers, lowers the density interface and so affects the water quality. We experimentally investigated the effect of aquatic plants on the turbulent flow from a mechanical disturbance in the closed water area. Results indicated that the presence of floating and submerged plants had a significant effect on the scale of the turbulent entrainment, and that the entrainment velocity depended on the overall Richardson number to the power of –3/2.     

Genotyping of the <Emphasis Type="Italic">SiDREB2</Emphasis> gene in worldwide foxtail millet (<Emphasis Type="Italic">Setaria italica</Emphasis> (L.) P. Beauv.) genetic resources with special attention to Indian landraces

We genotyped Setaria italica Dehydration-responsive element-binding 2 (SiDREB2) gene, which had been reported to be associated with dehydration stress tolerance, in 588 accessions of foxtail millet from various parts of Eurasia and other regions by a dCAPS marker. Of these, 480 accessions were genotyped in ribosomal DNA (rDNA), polyphenol oxidase (PPO) gene and Heading date 1 (HD1) gene in our previous studies, and 108 accessions from India were newly used in this study and genotyped in these genes in addition to the SiDREB2 gene. We compared the geographical distribution of the SiDREB2 genotypes with that of these three genes. Accessions from countries in South Asia including India and Sri Lanka were the most variable in the SiDREB2 gene followed by Korean accessions and Japanese accessions, suggesting that Indian accessions and East Asian accessions are useful as genetic resources for dehydration stress tolerance. This study also showed that although Indian accessions are not so diverse in rDNA and in transposon display markers previously studied, they are diverse in adaptive genes such as SiDREB2, PPO and HD1 genes.     

黑龙江省主栽水稻品种抽穗模型研究初报

采用黑龙江省生产上主栽的早熟、中熟和晚熟的6个水稻品种,在哈尔滨地区做分期栽培田间试验。调查记载播种、插秧和抽穗期。采取平行观测的气象资料,分析温度对水稻抽穗期和水稻营养生长期的影响。结果表明:不同的插秧期水稻抽穗期变化很大,不同熟期的水稻品种插秧至抽穗期需要的有效积温差别明显,但每个品种需要的有效积温很稳定。     

Diagnostic value of recombinant nanoluciferase fused Toxoplasma gondii antigens in Luciferase-linked Antibody Capture Assay (LACA) for Toxoplasma infection in pigs

Toxoplasmosis is a widespread protozoan zoonosis. Since ingesting undercooked meat harboring Toxoplasma gondii cyst is considered one of the major transmission routes to humans, the screening of T. gondii in meat-producing animals can reduce the risk of food-borne toxoplasmosis in humans. Among serological diagnostic methods, Luciferase-linked Antibody Capture Assay (LACA) has been found to be a promising platform with high sensitivity and specificity. In this study, we aimed to evaluate recombinant nanoluciferase fused-T. gondii antigens (rNluc-GRA6, rNluc-GRA7, rNluc-GRA8 and rNluc-BAG1) for their potential use in LACA for pigs. As a result, the sensitivity of GRA6-, GRA7-, GRA8- and BAG1-LACA were 70.0%, 80.0%, 80.0% and 30.0% with specificity 87.0%, 81.5%, 74.1% and 50.0%, respectively. The cocktail LACA using a mixture of rNluc-GRA6, rNluc-GRA7 and rNluc-GRA8 indicated higher sensitivity (90.0%) and a similar specificity (96.3%) in comparison with the commercial ELISA kit. Compared to the Dye-Test as a reference test, cocktail LACA showed strong agreement (kappa value=0.811) when we assessed pig sera collected at the slaughterhouse. In addition, we also successfully established the rapid LACA format for the detection of Toxoplasma infection in pigs (called Rapid-LACA) in which the test could be performed within 30 min. In Rapid-LACA, the protein A pre-coated/blocked plates could be preserved at −30°C, 4°C or room temperature conditions for at least two months without compromising on the quality of assay.