The objective of this study was to investigate the effects of diet composition on performance, slaughter yield and plasma metabolites, as different modern broiler strains show different responses to feed intake. Broilers of two commercial strains and of both sexes received one of three diets being different in energy and/or protein level [control diet, low energy/low protein diet (LM/LP) and low protein diet (LP)]. Low energy/low protein diet chickens were characterized by significantly lower body weights and feed intake compared with their LP and control counterparts. Broilers of the Cobb strain or broilers that were fed the control diet were most efficient in converting energy to body weight. No significant differences in plasma metabolites were detected due to diet composition or genotype. The diet with the lower energy and crude protein levels reached the lowest slaughter yield but the highest drumstick and wing percentages. The lowest mortality percentages were observed for broilers fed the LM/LP diet, and Cobb birds appeared to be more sensitive for metabolic disorders resulting in death. It is obvious from this study that different genotypes respond differently to changes in diet composition and therefore have adjusted nutritional requirements. 相似文献
We sampled and analyzed individually, edible dorsal muscle from largemouth bass (LMB), Micropterus salmoides (n?=?138) and yellow perch (YP), Perca flavescens (n?=?97) from 15 lakes to investigate potential local impacts of mercury emission point sources in northeastern Massachusetts (MA), USA. This area was identified in three separate modeling exercises as a mercury deposition hotspot. In 1995, 55% of mercury emissions to the environment from all MA sources came from three municipal solid waste combustors (trash incinerators) and one large regional medical waste incinerator in the study area. We determined the mercury accumulation history in sediments of a lake centrally located in the study area. Recent maximum mercury accumulation rates in the sediment of the lake of ~ 88 μg/m2/year were highly elevated on a watershed area adjusted basis compared to other lakes in the Northeast and Minnesota. Fish from the study area lakes had significantly (p?=?0.05) greater total mercury concentrations than fish from 24 more rural, non-source-impacted lakes in other regions of the state (LMB n?=?238, YP n?=?381) (LMB: 1.5–2.5 x; YP: 1.5 x). The integration of this extensive fish tissue data set, depositional modeling projections, historical record of mercury accumulation in sediments of a lake in the area, and knowledge of substantial mercury emissions to the atmosphere in the area support designation of this area as a mercury depositional and biological concentration hotspot in the late 1990s, and provides further evidence that major mercury point sources may be associated with significant local impacts on fisheries resources. 相似文献
1. The relationship between repeated thermal treatments and hepatic synthesis of Hsp 70 was studied in broiler chickens. 2. Sixty broilers were submitted to 5 different treatments (12 birds each) from day 1 to day 42. Four groups were kept in a thermoneutral environment and subjected to 0, 1, 2 and 3 heat stress episodes at 35 degrees C for 4 h per week (TN-0, TN-1, TN-2 and TN-3, respectively). The last group (HT-35) was reared at a room temperature of 35 degrees C. 3. From 39 to 42 old, the birds experienced acute heat stress at 41 degrees C. Resistance to heat stress was evaluated by the time taken for rectal temperature to increase by 3 degrees C above the pre-treatment value. Livers were collected (before and after heat stress) and Hsp70 was determined using Western Blot analysis with monoclonal anti-Hsp70 antibody. 4. Resistance to heat stress and concentration of Hsp70 were higher in those birds subjected to more heat stress episodes during the experimental period (TN-3) and HT-35. A positive correlation was observed between Hsp70 concentration and the time taken for a 3 degrees C increase in rectal temperature (r = 0.42; P<0.01). 5. Exposing birds to episodes of heat stress (35 degrees C) during rearing may improve their resistance to acute heat stress, but the previous thermal history did not seem to influence the hepatocyte Hsp70 content after exposure to more severe heat stress (41 degrees C). 相似文献
Ghrelin injection, either centrally or peripherally strongly stimulates feeding in human and rodents. In contrast, centrally injected ghrelin inhibits food intake in neonatal chickens. No information is available about the mechanism and its relationship with energy homeostasis in chicken. Since ghrelin is predominantly produced in the stomach, we investigated the effect of peripherally injected ghrelin (1 nmol/100g body weight) on food intake and energy expenditure as measured in respiratory cells by indirect calorimetry for 24h in one-week-old chickens. Plasma glucose, triglycerides, free fatty acids, total protein and T(3) were measured in a separate experiment until 60 min after injection. Food intake decreased until at least 1h after intravenous ghrelin administration. The respiratory quotient (RQ) in ghrelin-injected chickens was reduced until 14 h after administration whereas plasma glucose and triglycerides concentrations were not altered. Free fatty acids and total protein levels also remained unchanged. Ghrelin did not influence heat production and this was supported by the absence of changes in plasma T(3) levels when compared to the control values. In conclusion, peripheral ghrelin reduces food intake as well as RQ and might influence the type of substrate (macronutrient) that is used as metabolic fuel. 相似文献
Since the 1950s, invertebrate fisheries catches have rapidly expanded globally to more than 10 million tonnes annually, with twice as many target species, and are now significant contributors to global seafood provision, export, trade and local livelihoods. Invertebrates play important and diverse functional roles in marine ecosystems, yet the ecosystem effects of their exploitation are poorly understood. Using 12 ecosystem models distributed worldwide, we analysed the trade‐offs of various invertebrate fisheries and their ecosystem effects as well as ecological indicators. Although less recognized for their contributions to marine food webs, our results show that the magnitude of trophic impacts of invertebrates on other species of commercial and conservation interest is comparable with those of forage fish. Generally, cephalopods showed the strongest ecosystem effects and were characterized by a strong top‐down predatory role. Lobster, and to a lesser extent, crabs, shrimp and prawns, also showed strong ecosystem effects, but at lower trophic levels. Benthic invertebrates, including epifauna and infauna, also showed considerable ecosystem effects, but with strong bottom‐up characteristics. In contrast, urchins, bivalves, and gastropods showed generally lower ecosystem effects in our simulations. Invertebrates also strongly contributed to benthic–pelagic coupling, with exploitation of benthic invertebrates impacting pelagic fishes and vice versa. Finally, on average, invertebrates produced maximum sustainable yield at lower levels of depletion (~45%) than forage fish (~65%), highlighting the need for management targets that avoid negative consequences for target species and marine ecosystems as a whole. 相似文献
Contrast paranasal sinusography was a simple procedure that enhanced evaluation of the paranasal sinuses in the standing horse. Two techniques of positive-contrast paranasal sinusography were developed using cadavers and sedated horses. For both techniques, 37% organically bound iodine was used. For the first technique, the conchofrontal sinus was injected with 30 ml of contrast medium followed by an additional 100 ml and 70 ml. For the second technique, the rostral maxillary sinus was injected with 20 ml followed by an additional 50 ml. Each technique enhanced visualization of different paranasal sinuses, and each was necessary for complete positive-contrast radiographic evaluation of the paranasal sinuses. The techniques of positive-contrast radiographic evaluation of the paranasal sinuses are described. 相似文献
1. The pronounced episodic release of chicken growth hormone (cGH) in male broiler chicks, as well as its peak interval of about 90 min was confirmed in this study. 2. Lines selected for 6-week body weight (GL line) or for food conversion between 3 and 6 weeks of age (FC line) not only differ in the selection criteria but also in percentage abdominal fat. 3. The overall mean plasma GH concentration was higher in the FC line in both experiments, and this was only caused by peak amplitudes which were almost twice as high as in the FC line. Peak intervals were identical in both selected lines. 4. These observations suggest that decreased abdominal fat and increased protein efficiency may be genetically linked to increased plasma GH peak amplitude. This, and earlier observations on the effectiveness of pulsatile administration of cGH on the growth of broilers suggest that the biological function of GH may depend on its secretory pattern rather than its mean concentration in plasma. 相似文献
Tri-iodothyronine (T3) has been administered in the diet, from day of hatch until 8 weeks of age, to sex-linked dwarf and normal chicks of both sexes from a brown-egg slow-growing strain. Feed was supplemented with either 0.1 ppm or 0.5 ppm T3. A significant genotype by treatment interaction was observed on body weight: the effect of T3 in males was significantly positive for dwarfs and null for normals, the effect in females was null for dwarfs and significantly negative for normals. Feed efficiency was rather decreased by the treatment in both genotypes. Abdominal fatness was decreased in a dose-dependent manner in both genotypes, while rectal temperature was raised in dwarf chicks only. Plasma T3 was raised to normal levels in dwarfs receiving 0.1 ppm exogenous T3, while the 0.5 ppm dose yielded hyperthyroid levels. Plasma GH levels were decreased in a dose-dependent manner by the T3 treatment, the effect being larger in dwarfs. Surprisingly, plasma IGF-I was unchanged in spite of the GH decrease, whatever the genotype or the sex. It was concluded that exogenous T3 alone can have a stimulatory effect on growth in dwarfs but can not fully restore a normal growth rate. Both T3 and IGF-I are important for a normal growth and the relationships between T3 and IGF-I production should be further investigated in order to better understand the physiological modifications due to the sex-linked dwarf gene. 相似文献
1. Selection for leanness by selecting for food conversion (FC‐line) or selection for rapid growth and, thus, a concomitant fat increase (GL line) has also resulted in a lower egg laying rate of the fat birds. In birds of both lines that were effectively laying, plasma progesterone (P4), but not oestradiol concentrations were significantly different, with the lower concentration found in the GL line. This difference could not be attributed to differences in follicle population because this was similar in both groups of selected birds.
2. The in vivo decreased P4 secretion was investigated in vitro using granulosa cells of Fl follicles in a primary cell culture system. Basal P4 secretion tended to be lower in the GL line, whereas LH stimulation resulted in a diminished difference between the two lines. This was mainly caused by a higher relative P4 increase in the GL‐line.
3. Pre‐incubation with FSH increased the LH stimulated P4 secretion equally in both lines, probably through an increase of LH receptors.
4. The results demonstrate that selection for lean or fast growing, fat birds can also change processes apparently unrelated to the selection criteria, but with important effects on reproduction characteristics. 相似文献