蓟马对紫花苜蓿危害的空间分布特征

昆虫空间分布格局是其种群的一种客观属性,对于同一种群,其格局类型是固有的.蓟马为一种体型较小的昆虫,取食时移动性强,易逃逸,所以用危害特征的空间分布来代替其本身的空间分布更具有稳定性和实用性.采用不同尺度研究探讨蓟马对紫花苜蓿危害的空间分布特征.研究表明在现蕾期危害空间分布为聚集分布,危害斑块尺度是40 cm×30 cm～50 cm×30 cm的椭圆型,长轴方向是东西向.因此在这一物候期选择抗蓟马材料时,取样应该按南北方向,尺度距离大于40～50 cm,更具有代表性,在开花期危害空间分布为均匀分布,但是取样尺度也应大于40～50 cm,更具有代表性,而且在开花期取样要比现蕾期取样更好一些,会减少取样误差.     

Ancient diets, ecology, and extinction of 5-million-year-Old horses from florida

Six sympatric species of 5-million-year-old (late Hemphillian) horses from Florida existed during a time of major global change and extinction in terrestrial ecosystems. Traditionally, these horses were interpreted to have fed on abrasive grasses because of their high-crowned teeth. However, carbon isotopic and tooth microwear data indicate that these horses were not all C4 grazers but also included mixed feeders and C3 browsers. The late Hemphillian Florida sister species of the modern genus Equus was principally a browser, unlike the grazing diet of modern equids. Late Hemphillian horse extinctions in Florida involved two grazing and one browsing species.     

Switchable surfactants

Many industrial applications that rely on emulsions would benefit from an efficient, rapid method of breaking these emulsions at a specific desired stage. We report that long-chain alkyl amidine compounds can be reversibly transformed into charged surfactants by exposure to an atmosphere of carbon dioxide, thereby stabilizing water/alkane emulsions or, for the purpose of microsuspension polymerization, styrene-in-water emulsions. Bubbling nitrogen, argon, or air through the amidinium bicarbonate solutions at 65 degrees C reverses the reaction, releasing carbon dioxide and breaking the emulsion. We also find that the neutral amidines function as switchable demulsifiers of an aqueous crude oil emulsion, enhancing their practical potential.     

巴美肉羊INHA和INHBA基因多态性与产羔数关系研究

为了研究INHA和INHBA这两个基因对巴美肉羊产羔数的影响,采用PCR-SSCP技术检测抑制素α亚基(α-inhibin,INHA)和βA亚基(βA-inhibin,INHBA)基因在巴美肉羊中的单核苷酸多态性。结果发现,引物1,4,6扩增片段有多态性,其余3对引物的扩增片段均不存在多态性。引物1,巴美肉羊有2种基因型AB型和AA型,平均产羔数AB型比AA型多0.17只(P<0.01)。引物4,巴美肉羊存在3种基因型(CC、DD和CD),巴美肉羊平均产羔数DD型比CC型多0.44只(P<0.01)。引物6,巴美肉羊有3种基因型(EE、FF和EF),EE型比EF型平均产羔数多0.21只(P<0.01),结果表明,抑制素α亚基(α-inhibin,INHA)和βA亚基(βA-inhibin,INHBA)基因是影响巴美肉羊产羔数的重要基因。     

Analysis of polymorphisms of cathepsin B and cystatin B impact on economically important traits in pigs raised in Poland

The cathepsins belong to an enzyme family of lysosomal proteinases, which have a wide spectrum of function in a lot of tissues and cell types. Cathepsin B is one of intracellular proteases, whose role is to carboxydipeptidyl activity. In turn, the cystatin B (CSTB) is an intracellular thiol proteinase inhibitor. In pigs, CTSB was mapped on 14 chromosome and linked to loci genes ATP2A2, ACTN2 and ACTA1, in the region where suggestive QTL for fat deposition and meat content were identified. The CSTB gene is localized on telomeric end (1/2) q46–q49 of SSC13 and on this chromosome QTLs for daily gain and birth weight were identified. Our investigation concerned analysis of effects A72C CTSB polymorphism and Asp62Asn CSTB missense mutation on carcass traits in Polish pigs population. The significant results of A72C of CTSB mutation was observed for several growth traits in Pietrain pigs. AC genotype characterized higher carcass yield and weight of ham and loin than in AA pigs (AC — 79.3%, 6.90 kg and 10.2 kg; AA — 77.8, 6.52 and 9.89, P < 0.01). The significant effect of Asp62Asn CSTB was in two traits of Polish Large White pigs: AA animals had higher daily gain and lower number days in test compared to animals with GG genotype (AA — 973 g, 162 days; GG — 882 g, 172 days) with favorable additive genetic effects of allele g173A on LSM. The selection on A allele of CSTB should lead to increasing level of fat. In turn, increasing in population, a C allele of CTSB should affect better meat content parameters in pig population. Overall, these two polymorphisms seem not to be directly association with carcass traits, but probably are linked to unknown QTLs localized on 13 and 14 chromosomes.     

蒙古马睾丸支持细胞的体外分离培养与鉴定

为研究一种简单快速分离蒙古马睾丸支持细胞并保证其活性的基本方法,本试验采集2岁蒙古马睾丸组织于低温环境下进行机械分离,将1~3 g睾丸组织剪碎,采用重力沉淀法去除游离的红细胞和间质细胞;使用0.1%胶原酶Ⅳ和0.25%胰蛋白酶+EDTA逐步进行组织消化,并用含有10%胎牛血清的培养基进行细胞培养;在接种后采用差异贴壁法分离纯化支持细胞,使用Tris-HCl低渗法去除杂质细胞,并采用碱性磷酸酶（AKP）染色、HE染色、实时荧光定量PCR方法进行鉴定。结果显示,支持细胞贴壁性能较强,培养24 h后大多数细胞开始贴壁,细胞形状呈椭圆形;培养48 h后胞质增多,折光性变强;培养3~4 d细胞胞质展开紧密连接,此时细胞呈三角形,有明显的核仁,培养6~7 d细胞生长状态进入稳定期。实时荧光定量PCR结果显示,GATA4和GDNF基因在培养细胞中极显著表达（P<0.01）,AKP染色支持细胞呈阴性表达,表明分离培养的细胞确为支持细胞。本试验使用机械分离法与两步酶消化法处理组织,可快速高效地获得睾丸支持细胞,成功构建了蒙古马睾丸支持细胞体外培养方法。     

熔喷过程湍流模型中的气流场数值模拟

以熔喷过程中的气流场为核心,建立熔喷过程的数学物理模型,采用数值模拟的方法进行研究。以Navier-Stocks方程为基础,应用基于同位网格的SIMPLER算法并行化计算气流场的速度分布,将试验测试值与数值模拟进行比较,结果表明:采用的数值模拟方法是有效的,完全可以用于模拟双槽钝头型模具的熔喷过程。     

Einfluß von Monoethanolamin auf Ertrag und Wasserausnutzung von Sommergerste

Effect of monoethanolamine on yield and water use efficiency of barley The effect of monoethanolamine (EA) on yield and water use efficiency (WUE) of spring barley plants was tested in pot experiments. The application of EA (8 to 12 mg/pot) increased the grain yield and WUE by about 4 to 15 per cent under stress conditions. In addition, EA improved the utilization of fertilizer nitrogen. The yield-increasing effect of EA was associated with an increase of the number of ear-bearing tillers and an improved root growth. The effects of EA were only significant at α = 0.05.     

Towards a new gliadin reference material–isolation and characterisation

Twenty-eight wheat cultivars representative of the three main European wheat producing countries, France, UK and Germany, were selected as a source for the preparation of a reference gliadin. One kilogram of kernels from each cultivar were mixed and milled. The resulting white flour was defatted and vacuum dried. Albumins and globulins were eliminated by extraction using 0.4 M NaCl solution and gliadins were extracted with 60% ethanol. The gliadin extracts were concentrated, desalted by ultrafiltration, freeze-dried, and homogenised. After tests had shown good solubility and homogeneity, aliquots of the reference gliadin were sent to 16 different laboratories for further investigations: The material was analysed by various methods including RP-HPLC, SE-HPLC, RP-HPLC-ESI-MS, MALDI-TOF, capillary electrophoresis, acid-PAGE, 2D-PAGE, SDS-PAGE and immunoblotting and ELISA-tests with different monoclonal and polyclonal antibodies. The results showed that the gliadin composition of the source flour and the reference gliadin matched perfectly, demonstrating that no major gliadin components had been lost during the isolation procedure. The reference gliadin showed good immunochemical sensitivity with different gliadin antibodies in enzyme immunoassays. Because of its high protein and gliadin content, good solubility, homogeneity, stability and representative character, the product is regarded as a suitable universal reference material.