Acute infection of mice with Smith strain of Staphylococcus aureus

Two serologically distinct variants found in a unique strain of staphylococcus produce coagulase and are phagocytized, but only one is virulent to mice. Only virulent cocci grow rapidly within leukocytes. Leukocyte destruction by the virulent strain and release of many phagocytized cocci precedes mouse death. The leukocidic agent may be delta-hemolysin.     

Differential Rotation and Dynamics of the Solar Interior

Splitting of the sun's global oscillation frequencies by large-scale flows can be used to investigate how rotation varies with radius and latitude within the solar interior. The nearly uninterrupted observations by the Global Oscillation Network Group (GONG) yield oscillation power spectra with high duty cycles and high signal-to-noise ratios. Frequency splittings derived from GONG observations confirm that the variation of rotation rate with latitude seen at the surface carries through much of the convection zone, at the base of which is an adjustment layer leading to latitudinally independent rotation at greater depths. A distinctive shear layer just below the surface is discernible at low to mid-latitudes.     

Effects of Prolonged in vitro Culture and Cryopreservation on Viability,DNA Fragmentation,Chromosome Stability and Ultrastructure of Bovine Cells from Amniotic Fluid and Umbilical Cord

The objective of this work was to study cellular types that did not participated in the gastrulation process, amniotic fluid cells (AFCs) and umbilical cord cells (UCCs), in conditions of long‐term culture and cryopreserved with different solutions. The AFCs and UCCs were used in a comparative study with ear fibroblast cells (EFCs) that were cultured in vitro until 20 cellular passages and cryopreserved in 10% dimethylsulphoxide (DMSO), 5% dimethyl formamide (DMF) and 7% glycerol (Gly) solutions. The cellular viability, ultrastructure, DNA fragmentation and chromosome stability were evaluated to determine the cellular type most resistant. In all cell types, it was possible to evaluate the AFCs until 15 passages and UCCs until 20 passages with different periods of cellular growth to reach the confluence phase. Solutions containing 10% DMSO ensured viability of 90.33 ± 5.58%, 90.56 ± 4.40% and 81.90 ± 3.31%, respectively for EFCs, AFCs and UCCs, being significantly more efficient and with less variation than other cryoprotectant solutions. The AFCs were more sensitive to cryopreservation and presented low viability rate at the passage 20 (17.2 ± 8.87%). There was no change in karyotype and nuclear fragmentation was low in all cellular passages studied. With the scanning electron analysis was possible the characterization of AFCs and UCCs in suspension. The three cellular types of cells presented different shapes and characteristics on the surface. The results demonstrate that bovine AFCs and UCCs can be isolated, cultured in vitro and cryopreserved in 10% DMSO, not causing damage to DNA and chromosomes. The UCCs were more resistant than AFCs in all aspects.     

The influence of date of sowing and seed rate on the production of pure-sown red clover

Hungaropoly tetraploid broad red clover was sown at seed rates of 6, 12 or 18 kg ha^-1 on six dates from April to September 1971. Three crops were harvested in 1972 and one in June 1973. In 1972, total herbage dry matter yields ranged from 5.22 to 12.22 t ha^-1 and red clover dry matter yields from 3.61 to 11.92 t ha^-1 when meaned over all seed rates. April to July sowing dates gave significantly higher yields than later sowings. In general, August and September sowings gave the lowest red clover contents in a range from 63.2 to 96.5%, the highest digestibilities within a range 61.9 to 65.0% and the lowest crude protein contents in a range 15.5 to 17.3%. The influence of seed rate was less marked than sowing date. Mean annual yields of total herbage dry matter increased from 9.88 to 10.85 t ha^?1 as seed rate was increased from 6 to 18 kg ha^?1. Red clover dry matter yields and contents of red clover and crude protein in the total herbage also followed this trend. The sowing date effects on total herbage yield and content of red clover did not persist into the second harvest year but the seed rate effects were still noticeable. Plant numbers in spring the first harvest year and hence percentage survival from sowing were depressed by late sowing; plant numbers rose but percentage survival declined as seed rate was increased. The seed rate effects on plant population persisted until spring of the second harvest year but sowing date effects did not. Better stands of red clover were obtained from sowings made between April and June, when a seed rate of 12 kg ha^?1 was adequate. The adverse effects of late sowing cannot be fully compensated by raising seed rates of clover. There was a significant interaction between seed rate and date of sowing. For April-May sowings, seed rate was not critical. Thereafter, a linear effect of seed rate on yield was discernible.