Nitrogen metabolism in growing swine receiving a bacterial protein supplement (Alcaligenes eutrophus) instead of soybean meal

In a balance trial with 10 pigs (mean body mass 50 kg) the influence of a bacterial protein supplement (Alcaligenes eutrophus) on N-metabolism was investigated. The bacteria were included into the diet at levels of 7 and 14% at the expense of extracted soyabean meal. Thus bacterial "pure protein" (bacterial non-nucleic acid N X X 6.25) amounted to 30 and 60% of the protein of the ration. Consuming 2 kg of feed dry matter per day the animals of the control group (I) and the experimental groups (II and III) ingested 48 g, 52 g and 55 g of total N respectively. The difference in N-intake is explained by the additional nucleic acid-N, amounting to 19,8% of total bacterial N. Daily weight gain (on average 1054 g) and feed conversion efficiency (feed ingested/weight gain; on average 1,9) were relatively improved at the highest dietary level of bacterial cell mass. Faecal N-excretion was increased significantly, whereas renal N-excretion remained unchanged. Mean apparent N-digestibility was 87,4% showing no significant difference between the experimental groups. N-balance values were noticibly increased following the intake of the bacterial protein supplement. The excretion of urinary urea-N was slightly reduced whereas 4-6 times as much allantoin-N was excreted when bacteria were fed. It is calculated that about 80% of the bacterial purines are renally excreted as allantoin and uric acid.     

Intracranial schwannoma in a cow

A nine year old Hereford crossbred cow with a history of progressive neurological signs was referred to the Western College of Veterinary Medicine, Saskatoon. A large intracranial mass, histologically identified as a schwannoma, was found to be compressing the left brain stem and appeared to have arisen from the left fifth cranial nerve.     

Serotypes of Yersinia pseudotuberculosis recovered from domestic livestock

The serological identity of 234 strains of Yersinia pseudotuberculosis recovered from domestic animals and birds in New Zealand was determined by slide agglutination test. Thirty strains were also examined by tube agglutination test. The strains were isolated from cattle (56), sheep (8), deer (117), goats (13), pigs (7), rabbits (6), guinea pigs (5), and aviary species of birds (22). All strains were isolated from animals or birds which had died or shown signs of ill health and amongst which diarrhoea was a common feature. Serotype I accounted for 23% (53) of strains, serotype II for 13% (30) of strains and serotype III for 64% (151) of strains. It was concluded that further investigations on the prevalence and serological identity of strains recovered from clinically healthy animals mav provide useful information in assessing the significance of various serotypes as a cause of disease in livestock.     

Characterization of staphylococci associated with clinical and subclinical bovine mastitis

Attempts were made to identify 900 species of staphylococci or micrococci recovered from samples of bovine milk examined for mastitis pathogens. The presence and identity of haemolysins was recorded together with results of disc diffusion antibiotic sensitivity tests. The occurrence of clinical mastitis was also noted and somatic cell counts (SCC) were performed on milk samples which were normal in appearance. Eight hundred and thirty-one coagulase positive staphylococci were obtained, of which 810 were S. aureus and 21 were S. intermedius. Of 65 coagulase negative staphylococci the species of 19 could not be determined by the identification systems used. The remainder were identified as S. hyicus sub sp. hyicus (1), S. hyicus sub sp. chromogenes (19), S. haemolyticus (17), S. hominis (3), S. epidermidis (4), S. capitis (1) and either S. hominis or S. warneri (1). Four other isolates could not clearly be assigned to the genus Staphylococcus or Micrococcus and were designated irregular strains. No micrococci were identified. The presence of alpha, beta, or delta haemolysins occurring singly or in various combinations was identified in 98.3% of coagulase positive staphylococci and in 60% of coagulase negative staphylococci. Epsilon haemolysin was detected in 47.6% of the coagulase negative staphylococci and in 9.5% of S. intermedius. All staphylococci were sensitive to tetracycline (30 microg), novobiocin (1.6 microg), nafcillin (30 microg), methicillin (10 microg) and cephalothin (30 microg) and variable numbers of each species were sensitive to penicillin (2 iu) and streptomycin (10 microg). One non-identified species of coagulase negative staphylococcus was sensitive to erythromycin (0.4 microg) the remaining staphylococci were resistant. Each of the four irregular strains was sensitive to erythromycin and novobiocin. Clinical mastitis was associated with 30.6% of coagulase positive staphylococci, 15.3% of coagulase negative staphylococci, and two of the four irregular strains (50%). Subclinical mastitis as determined by SCC of 500 x 10(3) or greater was associated with 92.7% of coagulase positive and 37.5% of coagulase negative staphylococci.     

Materials: unidirectional solidification of cast iron

The segregation of sulfur in liquid cast iron was studied under conditions of microgravity on Spacelab 1. A rod of cast iron containing carbon, silicon, sulfur, and phosphorus was unidirectionally solidified at four different rates. The influence of sulfur on the graphite structure and the stability of an aluminum oxide skin deposited on the surface of the specimen were investigated.     

Effects of pretreatment conditions on ammonium and nitrate uptake by Douglas-fir seedlings

Effects of pretreatment solutions containing varying concentrations of calcium, potassium, ammonium and nitrate were evaluated by measuring fluxes of the same ions during a subsequent 4-hour uptake by Douglas-fir seedlings. Maximal rate of ammonium uptake (11 microeq g(-1) root dry weight h(-1)) was about 5 times faster than that of nitrate (2 microeq g(-1) root dry weight h(-1)). Ammonium uptake was most rapid after pretreatment with low potassium levels and was unaffected by ammonium pretreatment. Nitrate uptake was most rapid after pretreatment with high levels of nitrate and low levels of potassium. Calcium uptake was greater when nitrate replaced ammonium as the N source. High calcium pretreatment levels depressed subsequent calcium uptake or resulted in calcium release in both ammonium and nitrate experiments. Potassium efflux occurred with both N sources, but the release was less during nitrate uptake than during ammonium uptake. Efflux of potassium is probably associated with the high potassium status of the seedlings and the exchange between potassium and other cations. High levels of potassium in the pretreatment solutions enhanced potassium efflux and caused a reduction in the subsequent rates of uptake of both ammonium and nitrate. Length of pretreatment and seedling size generally did not affect uptake rates.