Phenotypic and genetic characterization of enteropathogenic Escherichia coli (EPEC) and entero-aggregative E. coli (EAEC) from diarrhoeal and non-diarrhoeal children in Libya

A total of 50 Escherichia coli strains isolated in a Libyan hospital (20 from children with diarrhoea and 30 from healthy children) were investigated for their pathotypes and virulence traits. Altogether nine eae-positive (enteropathogenic E. coli, EPEC) and nine aggR-positive (entero-aggregative E. coli, EAEC) strains were identified. Significantly (P=0.001) more EPEC strains were identified from diarrhoeal patients (n=8) than from healthy controls (n=1), while six EAEC strains were identified from diarrhoeal and three from healthy children. Typical (eae(+), EAF(+), bfp(+)) EPEC strains (n=6) belonged to classical EPEC serogroups O55, O114, O127 and showed localized adherence on Hela cells. EAEC strains revealed genetic heterogeneity but uniformly adhered to HeLa cultures in an entero-aggregative adherence pattern. Antibiotic resistance frequently, characterized the strains. Sixty-eight percentage of the strains were resistant against at least one antibiotic and 30% harbored a class 1 integron independently of their clinical background. This is the first report from North Africa demonstrating the significance of EPEC and EAEC.     

Long-established and emerging pesticide poisoning in horses

Poisoning is a frequent problem in domestic animals. Horses are the third most commonly affected species and pesticides are one of the main causes of toxicosis. Here within are reviewed the most frequent toxicoses where it emerges that the main causes are misuse and accidental exposure. Rodenticides, acetylcholinesterase insecticides followed by herbicides are the main cause of toxicosis and effects of emerging chemicals are being reported. Metaldehyde is still a problem, together with methiocarb. No information has been reported on poisoning with pesticide mixtures.     

Salsolinol is present in the bovine posterior pituitary gland and stimulates the release of prolactin both in vivo and in vitro in ruminants

The aims of the present study were to determine whether salsolinol (SAL), a dopamine-related compound, is present in the bovine posterior pituitary (PP) gland, and to clarify the effect of SAL on the secretion of prolactin (PRL) in ruminants. SAL was detected in extract of bovine PP gland using high-pressure liquid chromatography with electrochemical detection (HPLC-EC). A single intravenous (i.v.) injection of SAL (5 and 10mg/kg body weight) significantly and dose-dependently stimulated the release of PRL in goats (P<0.05). Plasma PRL levels reached a peak 10min after the injection, then gradually returned to basal values in 60-80min. The PRL-releasing pattern was similar to that in response to sulpiride (a dopamine receptor antagonist). The intracerebroventricular (i.c.v.) injection of 1mg of SAL had no significant effect on the release of PRL in calves, however, 5mg significantly stimulated the release (P<0.05) with peak values reached 30-40min after the injection. Moreover, SAL significantly stimulated the release of PRL from cultured bovine anterior pituitary cells at doses of 10(-6) and 10(-5)M, compared to control cells (P<0.05). Taken together, our data clearly show that SAL is present in extract of the PP gland of ruminants, and has PRL-releasing activity both in vivo and in vitro. Therefore, this endogenous compound is a strong candidate for the factor having PRL-releasing activity that has been previously detected in extract of the bovine PP gland.     

Different reactions of central and marginal provenances of Fagus sylvatica to experimental drought

Climate extremes are expected to increase in frequency and magnitude as a consequence of global warming, threatening the functioning, services and goods of forest ecosystems. Across Europe, the ecologically and economically important tree species Fagus sylvatica is expected to suffer particularly under such conditions. The regional introduction of provenances from drier and warmer climates is one option to adapt beech forest ecosystems to these adverse effects of climate change. Marginal populations from the drought-prone southern and north-eastern edges of the species’ distribution come into focus in search of suitable candidates for Central European deciduous forests. Here, we test three marginal provenances (Spain, Bulgaria and Poland) and three provenances from the centre of the distribution range (Germany) for their response to drought in two different soil types (sand, loam) in a full factorial common garden experiment in Landau, Germany. Drought impacted all growth parameters negatively (leaf damage +22 % (percentage points), height ?40 % and diameter increment ?41 %), and the sandy substrate exacerbated this effect. However, provenances differed in their response to drought and soil type. Evidence for a local adaptation to summer drought was detected, especially in terms of mortality rates. The Bulgarian and Spanish provenance showed a stable performance under drought conditions (BG ?27 % in diameter increment; ES ?32 %), compared to the Polish (?48 %) or the most sensitive German provenances (?57 %), yet for Bulgaria on a low level of total increment. This may indicate a trade-off between drought tolerance and growth. Therefore, a sole focus on drought-resistant marginal provenances seems to not be conducive, as they might be less adapted to other climatic factors, e.g. frost, as well. However, intermixed with local Central European provenances, these may act as functional insurance in future drought-prone forest stands.     

Effects of alternative management practices on the economics,energy and GHG emissions of a wheat–pea cropping system in the Canadian prairies

In recent years alternative farming practices have received considerable attention from Canadian producers as a means to improve their net return from grain and oilseed production. Enhancing the efficiency of nitrogen fertilizer use, including a pulse crop in the rotation, reducing tillage and pesticide use are seen as viable options to reduce reliance on fossil fuel, lower input costs and decrease the risk of soil, air and water degradation. The objective of this study was to determine the effects of 16 alternative management practices for a 2-year spring wheat (Triticum aestivum L.)–field pea (Pisum sativum L.) rotation on economic returns, non-renewable energy use efficiency, and greenhouse gas emissions. The alternative management methods for wheat consisted of a factorial combination of high vs. low soil disturbance one pass seeding, four nitrogen (N) fertilizer rates (20 kg N ha^?1, 40 kg N ha^?1, 60 kg N ha^?1 and 80 kg N ha^?1), and recommended vs. reduced rates of in-crop herbicide application. Alternative management practices for field pea were high vs. low soil disturbance one pass seeding. The resulting 16 cropping systems were evaluated at the whole farm level based on 4 years (two rotation cycles) of data from field experiments conducted on two Orthic Black Chernozem soils (clay loam and loam textures) in Manitoba, Canada. The highest net returns on the clay loam soil were for the high disturbance system with 60 kg N ha^?1 applied to wheat and the recommended rates of in-crop herbicides. The lowest application rate of N, together with low disturbance seeding, provided the highest economic returns on the loam soil. Energy use efficiency was highest for the lowest rate of N application for both tillage systems. The highest rate of N fertilizer and recommended rates of in-crop herbicide produced little additional yield response, lower net returns, and higher GHG emissions. An increase in N fertilizer application from 20 kg ha^?1 to 80 kg ha^?1 increased whole farm energy requirements by about 40%, while reducing herbicide rates had negligible effects on grain yields and total energy input. Overall, as N fertilizer rate increased, the associated GHG emissions were not offset by an increase in carbon retained in the above-ground crop biomass. Moderate to high soil test NO₃-N levels at experimental sites reduced the potential for positive yield responses to N fertilizer in this study, thus minimizing the economic benefits derived from N fertilizer application.     

Tick-borne encephalitis epidemic in Hungary 1951–2021: The story and lessons learned

The authors analysed epidemiological data of the Hungarian tick-borne encephalitis epidemic from the past seven decades. A total of 911 meningitis serosa cases were described from 1930-1950 s by local hospital physicians, indicating that the virus had been present in the country decades before its official identification in 1952. The virus spread freely in the 1950s–1960s, occupying almost all habitats where ticks occurred in large numbers. The increasing number of cases drove authorities to classify this illness as a notifiable disease in 1977 and to organize the first measures to stop the epidemic. Statistical analysis revealed that the large-scale vaccination launched from the 1990s was responsible for the sharp decrease in the number of human cases from 1997. A significant negative correlation was found between the number of vaccine doses sold and human cases 6 years later. The TBEV endemic area covers 16.57% of the territory and 16.65% of the population of the country. In the last 10 years, 186,000 vaccine doses/year in average were enough to keep the incidence of human TBEV infections between 0.45 and 0.06/100,000 persons. A 20-year-long study found evidence for easing clinical signs in TBEV-infected hospitalized patients. Statistics found a sharp decrease in the number of samples sent for TBEV diagnosis after 1989. Male dominance of patients was characteristic of the epidemics since the 1940s, but now analysis of detailed data from the 1981–2021 period (60.5%–87.5%) proved the statistical significance of this dominance. Obviously, the voluntary vaccination programme was the tool which broke the spread of the epidemic. Widespread public awareness of the disease and the tick vector, probable evolutionary spread of less pathogenic virus strains supplemented with the vaccination campaign led to a negligible level of human TBE cases in Hungary in the last years.     

Effects of input management and crop diversity on non-renewable energy use efficiency of cropping systems in the Canadian Prairie

Although producers’ prime objective may be to increase net returns, many are also interested in conserving and enhancing the quality the soil, water and air resources through adopting more environmentally friendly production practices. This study compared non-renewable energy inputs, energy output, and energy use efficiency of nine dryland cropping systems comprised of a factorial combination of three methods of input management [high (HIGH), i.e., conventional tillage plus full recommended rates of fertilizer and pesticides; reduced (RED), i.e., conservation tillage plus reduced rates of fertilizer and pesticides; and organic (ORG), i.e., conventional tillage plus N-fixing legumes and non-chemical means of weed and pest control]; and three crop rotation systems with varying levels of cropping diversity [a fallow-based rotation with low crop diversity (LOW); a diversified rotation using annual cereal, oilseed and pulse grains (DAG); and a diversified rotation using annual grains and perennial forages (DAP)]. The study was conducted over the 1996–2007 period on a Dark Brown Chernozemic soil (Typic Boroll) in the Canadian Prairies. As expected, total direct plus indirect energy input was the highest for the HIGH and RED input treatments (3773 MJ ha^?1 year^?1), and 50% less for ORG management. Most of the energy savings came from the non-use of inorganic fertilizers and pesticides in the ORG management treatments. Further, total energy use was the highest for the DAG treatments (3572 MJ ha^?1 year^?1), and similar but about 18% lower for the DAP and LOW crop diversity treatments compared to DAG. Thus, overall, the HIGH/DAG and RED/DAG systems had the highest energy requirements (4409 MJ ha^?1 year^?1) and ORG/DAP had the lowest (1806 MJ ha^?1 year^?1). Energy output (calorimetric energy content) was typically the highest for the HIGH input treatments (26,541 MJ ha^?1 year^?1), was about 4% less with RED, and 37% less with ORG management. The latter reflected the lower crop yields obtained with organic management. Similarly, energy output was the highest for the DAP treatments (25,008 MJ ha^?1 year^?1), about 5% less for DAG, and 20% less for the LOW crop diversity treatments. The higher energy output with the DAP treatments largely reflected that the entire harvested biomass of the forage crops was included in energy output, while for grain crops only the seed was included. The straw and crop residues from annual crops were returned to the land to protect the soil from erosion and to maintain soil organic matter as this is the recommended practice in this semi-arid region. In contrast to energy output and to net energy produced, energy use efficiency (measured as yield of grain plus forage produced per unit of energy input or as energy output/energy input ratio) was the highest for the ORG input treatments (497 kg of harvested production GJ^?1 of energy input, and an energy output/energy input ratio of 8.8). We obtained lower, but generally similar energy use efficiency for the HIGH and RED input treatments (392 kg GJ^?1 and ratio of 7.1). Thus, overall, ORG/DAP was the most energy efficient cropping system, while RED/LOW and RED/DAG generally ranked the lowest in energy use efficiency. Our findings support the current movement of producers toward ORG management as a means of reducing the reliance on non-renewable energy inputs and improving overall energy use efficiency of their cropping systems. Our results also suggest that moving away from traditional monoculture cereal rotations that employ frequent summer fallowing, toward extended and diversified crop rotations that use reduced tillage methods, although resulting in an increase in energy output, will not significantly reduce the overall reliance on non-renewable energy inputs, nor enhance energy use efficiency, unless perennial legume forages and/or legume grain crops are included in the cropping mix.