Fibrinogen level in clinically healthy horses]

Various sets of horses were examined in view of the necessity of widening the range of biochemical substances for diagnostic purposes in the field of veterinary medicine. The aim of the investigations was to obtain basic information on fibrinogen level and its variability. The average value stated was 280 mg% s = 89, sx = 12, V% = 32). The fibrinogen level in horses of the pronounced oxidation type was lower than in those with reduced metabolism, the difference being connected with the speed of sedimentation of erythrocytes. Repeated examination of a part of the set of horses (n = 10) on three different days within one week under the same conditions, showed a nonsignificant difference in the variability of the investigated horses (F = 0.901); neither did the coefficient of repeatability (rop = 0.11) reach the level of the testing criterion. The results indicate variability, with normal physiological condition of the animal, in the fibrinogen level. The physiological variability in fibrinogen level can also be explained in connection with other protein fractions.     

An evaluation of anthelmintic properties,assessed using faecal nematode egg counts,of New Zealand native flax (Phormium tenax)

AIM: To investigate the anthelmintic properties of New Zealand native flax (Phormium tenax) for cattle, using a faecal egg count reduction test (FECRT).

METHODS: Twenty-six heifer calves with high (>300 epg) faecal nematode egg counts (FEC) were paired into two groups and fed either chopped flax leaves ad libitum, or barley straw at an equivalent dry matter (DM) rate, from Day 0–7; all were supplemented with 1.5 kg/head/day cereal feed supplement. On Days 8–10, all heifers were fed a common diet of 3.5 kg DM/ head/day barley straw and 1.5 kg/head/day cereal feed supplement. Heifers were weighed and faecal samples were collected on Days —6, 0, 7, and 10; samples were analysed in triplicate for FEC. The nutritive value of the flax and straw was analysed.

RESULTS: Both the flax and straw had low dry matter digestibility (DMD) and protein content. Although the flax-fed claves grew more than the controls, this may have been related to gut fill. Total daily faecal egg output was similar for flax and straw groups on Day 7 (14.7 vs 15.0 x 10⁶ eggs/day, respectively) and Day 10 (14.9 vs 15.1 x 10⁶ eggs/day, respectively). There was no difference in the change in FEC with time between the calves fed flax or straw diets.

CONCLUSION: Consumption of flax leaves did not reduce FEC in calves with a mixed nematode infection.     

Evaluation of variability of duration of pregnancy in mares

In a set of mares of English Thoroughbred horse of the Napajedla stock the gravidity length in the time period from 1880 to 1972 was evaluated. The variability of the gravidity length was evaluated in sets which were put together by combination of two age groups with a division of the studied time period into ten-year stages. Variance and sample means of the gravidity lengths in these sub-groups are significantly different in the period under study. In order to eliminate the action of non-genetic influences qualifying the variability of the gravidity length, and to increase the objectivity of their evaluation it is proposed not to calculate with absolute values of the gravidity length, but on the contrary with transformed values: (xi - x) divided by s which would be computed for corresponding year of the foaling period. Another methodical approach to exclude the variability of the gravidity length depending on seasonal influences was based on the use of correction factors which were determined for individual months of foaling, on the basis of the sinusoid form of the curve of the gravidity length in the course of the calendar year. When observing the influence of the age of sires on the gravidity length, the shortening of the gravidity length in dependence on their higher age has been suggested, however this relation was significant only sporadically. The submitted work is a part of a larger theme referring, to the reproduction of horses.     

Cefquinome Concentrations in Endometrium after Intrauterine Treatment of Cobactan 4.5%® in Mares and Inflammatory Response of the Endometrium to this Treatment

This study was conducted to measure the concentration of cefquinome in the endometrium of mares after intrauterine treatment and to evaluate associated inflammation. Mares (n = 14) were randomly assigned to one of the following groups: (i) control (n = 4) were either not treated (n = 2) or received (n = 2) lactated Ringer's intrauterine for 1 or 3 days; (ii) treated mares (n = 10) received intrauterine cefquinome for 1 or 3 days. After at least 10 days had passed following the last treatment and ovulation, mares were given Prostaglandin F2α (PGF2α) and were randomly assigned to an alternate treatment. Endometrial biopsy samples were taken at 2, 8, 24 and 48 h, or at 4, 12 and 36 h, after the last treatment. Biopsy samples were taken at the same time points from control mares (n = 2) and lactated Ringer-treated mares (n = 2). Cefquinome concentrations were quantified using a high-performance liquid chromatography (HPLC) assay and inflammation was assessed using haematoxylin and eosin (H&E)-stained sections. Concentrations of cefquinome [559 (1 day) and 595 μg/g (3 days) at 2 h, and 403 (1 day) and 370 μg/g (3 days) at 4 h] were similar between treatment groups at 2 and 4 h after treatment (p > 0.05). At 8 h, as well as at 24 and 48 h, concentrations were greater in the 3-day group (17 vs 301 μg/g, 3 vs 80 μg/g and 0.1 vs 0.2 μg/g, respectively) (p < 0.05). No significant differences (p > 0.05) in the inflammatory response at 2–48 h after treatment were found between groups.