PRODUCTION,CHEMICAL QUALITY AND USE OF DUCKWEEDS (LEMNACEAE) IN AQUACULTURE,WASTE MANAGEMENT,AND ANIMAL FEEDS

The vascular plants known as duckweeds have been the subject of intense interest during the past decade as an aquacultural crop, part of waste management systems, and as a feed supplement for aquatic and terrestrial animal stocks. Yields in high nutrient lagoons in regions with nine-month growing season average about 10 metric tons (dry)/hectare, with maximum yields of 12 metric tons projected from .04 hectare lagoons. Crude protein for duckweeds cultured on animal waste lagoons has ranged from 35 to 45% of the dry weight and all essential amino acids are present. Lysine is present in sufficient concentration to meet FAO reference standards for animal feeds, and methionine equals or exceeds recommended standards in specific clones of Lemna turionifera, L. minor, and Spirodela polyrrhiza. Minerals, fat, fiber, and carbohydrate levels are not excessive for most animal diets. Successful feeding trials have been conducted with several species of fish, ducks, swine, poultry, and cattle. A review of research indicates this family of plants holds high promise for producing high quality animal and human food world-wide, but the technology for controlled production under full-scale systems is incomplete.     

The influence of nonlinear mesoscale eddies on near-surface oceanic chlorophyll

Oceanic Rossby waves have been widely invoked as a mechanism for large-scale variability of chlorophyll (CHL) observed from satellites. High-resolution satellite altimeter measurements have recently revealed that sea-surface height (SSH) features previously interpreted as linear Rossby waves are nonlinear mesoscale coherent structures (referred to here as eddies). We analyze 10 years of measurements of these SSH fields and concurrent satellite measurements of upper-ocean CHL to show that these eddies exert a strong influence on the CHL field, thus requiring reassessment of the mechanism for the observed covariability of SSH and CHL. On time scales longer than 2 to 3 weeks, the dominant mechanism is shown to be eddy-induced horizontal advection of CHL by the rotational velocities of the eddies.     

Evaluation of a bladder tumor antigen test as a screening test for transitional cell carcinoma of the lower urinary tract in dogs

OBJECTIVE: To evaluate the veterinary version of the bladder tumor antigen (V-BTA) test as a screening test for transitional cell carcinoma (TCC) of the lower urinary tract of dogs. ANIMALS: 229 client-owned dogs. PROCEDURE: Urine samples from dogs were shipped overnight to a single laboratory to facilitate testing within 48 hours of collection by use of the V-BTA rapid latex agglutination urine dipstick test. Groups of dogs included the following: 1) dogs with TCC of the lower urinary tract, 2) healthy control dogs, 3) unhealthy control dogs with non-TCC urinary tract disease, and 4) unhealthy control dogs without urinary tract disease. Test sensitivity and specificity were calculated by use of standard methods. Logistic models were developed to assess the effect of disease status, test conditions, urine composition, and signalment on the performance of the V-BTA test. RESULTS: A total of 229 urine samples were analyzed, including 48 from dogs with suspected (n = 3) or confirmed (45) TCC. Test sensitivities were 88, 87, and 85% for all dogs with (suspected and confirmed) TCC, dogs with confirmed TCC at any site, and dogs with confirmed TCC of the urinary bladder, respectively. Test specificities were 84, 41, and 86% for healthy control dogs, unhealthy control dogs with non-TCC urinary tract disease, and unhealthy control dogs without urinary tract disease, respectively. The test performed slightly better on centrifuged urine samples than on uncentrifuged urine samples. CONCLUSIONS AND CLINICAL RELEVANCE: Our results indicate that the V-BTA test is useful in screening for urinary tract TCC in dogs.     

Comparison of Two Methods for Presurgical Disinfection of the Equine Hoof

OBJECTIVES: To determine for equine hooves the normal resident aerobic bacterial population and the efficacy of 2 methods of disinfection. Study Design-Measurement of total bacterial, gram-positive bacterial, and gram-negative bacterial surface populations from the frog, sole, and hoof wall after each step of 2 different preoperative surgical disinfection techniques. ANIMALS: Six adult horses. METHODS: Hoof wall, sole, and frog samples were collected for quantitative bacteriology before, during, and after 2 multistep antiseptic preparation techniques: Method A-6-minute scrub with povidone-iodine soap, followed by 24-hour submersion in povidone-iodine solution-soaked cotton; and Method B-initial removal of superficial layer of hoof capsule before completing Method A disinfection procedures. RESULTS: Removal of the superficial hoof layer, application of the povidone iodine scrub, and completion of the povidone-iodine soak all significantly (P < .0008) decreased total bacterial numbers. Method B had significantly lower bacterial counts than method A at each consecutive step. Final total bacterial counts remained greater than 10(5) bacteria per gram of tissue regardless of preparation method. CONCLUSIONS: The hoof surface hosts a broad spectrum of aerobic gram-positive and -negative bacteria, many of which are potential pathogens. Bacterial numbers can be significantly reduced by removal of the superficial hoof surface, by application of a povidone-iodine scrub, and by use of a 24-hour povidone-iodine soak. However, bacterial populations >10(5) g per tissue persist after these disinfection procedures. CLINICAL RELEVANCE: Regardless of the preparation methods used in this study, bacterial populations capable of inducing wound infection remain on the hoof capsule.     

THE ROLE OF ULTRASOUND IN THE ASSESSMENT OF LARYNGEAL PARALYSIS IN THE DOG

Forty dogs with clinical signs suggestive of upper respiratory tract disease underwent echolaryngography and laryngoscopy. Laryngoscopy was used as the definitive technique to diagnose laryngeal paralysis. The ultrasound investigation accurately indicated the presence of the paralysis and confirmed the uni- or bilateral nature of the disorder. Findings indicative of laryngeal paralysis included asymmetry or absence of motion of the cuneiform processes (30/30), abnormal arytenoid movement (16/30), paradoxical movement (9/30), caudal displacement of the larynx (2/30) and laryngeal collapse (1/30). Thirty dogs were found to be afflicted with laryngeal paralysis and ten had normal laryngeal motility.