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31.
William R. Wengeler Douglas A. Kelt Michael L. Johnson 《Biological conservation》2010,143(5):1144-1153
The introduction of lake trout (Salvelinus namaycush) to Yellowstone Lake in Yellowstone National Park has contributed to a significant decline in the endangered Yellowstone cutthroat trout (Oncorhynchus clarki bouvieri), leading to concern over the persistence of this subspecies but also to piscivorous predators in this community. We assessed the impact of lake trout on a key piscivore, the river otter (Lontra canadensis) in two lakes in Yellowstone National Park. Yellowstone Lake continues to support a native cutthroat trout population, although the recent introduction of lake trout has dramatically impacted the cutthroat trout population. Nearby Lewis Lake has an entirely introduced fish fauna of lake trout, brown trout (Salmo trutta), and Utah chub (Gilia atraria) but lacks cutthroat trout. Analysis of otter scat from Yellowstone Lake implicated trout (lake or cutthroat trout) as a major prey item (57% of scat), whereas stable isotopes identified longnose suckers (Catostomus catostomus) as the primary prey there (58% of diet). By contrast, scat from Lewis Lake implicated minnows, presumably Utah chub, as the primary prey for otters occupying that lake (86%), while stable isotopes implicated brown trout (64%) over both lake trout and Utah chub (36% combined). Our data establish the importance of alternative prey to otters and suggest that lake trout-induced reductions in cutthroat trout may not be catastrophic for otter populations here. These data do not necessarily exonerate lake trout, as their impact on other species, most notably grizzly bears (Ursus arctos) and piscivorous birds, has been documented to be substantial, and further data on the nutritional value of alternate prey are required to confirm or refute a working hypothesis that otter populations will continue to thrive in the face of the lake trout invasion. 相似文献
32.
Georg Jentschke Eberhard Fritz Petra Marschner Christine Rapp Volkmar Wolters Douglas L. Godbold 《植物养料与土壤学杂志》1997,160(2):317-321
Non-inoculated spruce seedlings (Picea abies Karst.) and spruce seedlings colonized with Lactarius rufus Fr., Pisolithus tinctorius Coker & Couch or Paxillus involutus Fr. were grown for 35 or 37 weeks in a microcosm system on two types of natural forest humus differing in Pb content. Using X-ray microanalysis, the distribution and content of Pb in the tissues of mycorrhizal and non-mycorrhizal root tips were compared. No significant difference in the Pb contents of root cortex cell walls of non-mycorrhizal and seedlings colonized by Lactarius rufus, Pisolithus tinctorius, or indigenous mycorrhizal fungi (mainly Tylospora sp.) was found. However, in root tips of seedlings colonized by Paxillus involutus, due to a higher binding capacity for cations, the Pb content in cell walls of the root cortex were higher than in non-mycorrhizal roots. Pb contents in cell walls of the cortex of mycorrhizal and non-mycorrhizal roots were 3 times higher in plants growing in humus with a high Pb content than in plants growing in humus with a low Pb content. It is concluded that increasing contents of Pb in the organic matter may lead to an increased loading of the apoplast with Pb. The mycobionts tested in this investigation did not exclude Pb from root tissues. 相似文献
33.
Changelian PS Flanagan ME Ball DJ Kent CR Magnuson KS Martin WH Rizzuti BJ Sawyer PS Perry BD Brissette WH McCurdy SP Kudlacz EM Conklyn MJ Elliott EA Koslov ER Fisher MB Strelevitz TJ Yoon K Whipple DA Sun J Munchhof MJ Doty JL Casavant JM Blumenkopf TA Hines M Brown MF Lillie BM Subramanyam C Shang-Poa C Milici AJ Beckius GE Moyer JD Su C Woodworth TG Gaweco AS Beals CR Littman BH Fisher DA Smith JF Zagouras P Magna HA Saltarelli MJ Johnson KS Nelms LF Des Etages SG Hayes LS Kawabata TT 《Science (New York, N.Y.)》2003,302(5646):875-878
Because of its requirement for signaling by multiple cytokines, Janus kinase 3 (JAK3) is an excellent target for clinical immunosuppression. We report the development of a specific, orally active inhibitor of JAK3, CP-690,550, that significantly prolonged survival in a murine model of heart transplantation and in cynomolgus monkeys receiving kidney transplants. CP-690,550 treatment was not associated with hypertension, hyperlipidemia, or lymphoproliferative disease. On the basis of these preclinical results, we believe JAK3 blockade by CP-690,550 has potential for therapeutically desirable immunosuppression in human organ transplantation and in other clinical settings. 相似文献
34.
Machine learning was applied for the automated derivation of causal influences in cellular signaling networks. This derivation relied on the simultaneous measurement of multiple phosphorylated protein and phospholipid components in thousands of individual primary human immune system cells. Perturbing these cells with molecular interventions drove the ordering of connections between pathway components, wherein Bayesian network computational methods automatically elucidated most of the traditionally reported signaling relationships and predicted novel interpathway network causalities, which we verified experimentally. Reconstruction of network models from physiologically relevant primary single cells might be applied to understanding native-state tissue signaling biology, complex drug actions, and dysfunctional signaling in diseased cells. 相似文献
35.
The ability of plants to utilize P efficiently is important for crops growing in P‐deficient soils or on soils with a high P‐fixing capacity. The purpose of this work was to investigate early physiological changes which occur when wheat (Triticum aestivum L.) seedlings were grown under P‐deficient conditions. Wheat plants were grown in a greenhouse and watered with nutrient solution containing or lacking P. During the interval 12 to 18 days after planting, the dry weight of wheat seedlings was similar regardless of P treatment, although the P‐deficient plants had a greater proportion of the total plant weight in the roots. Sixteen days after planting, the roots and leaves of P‐deficient plants had only 20 to 30% the P content of P‐sufficient plants. After 16 days, plants grown under P stress had 41% more p‐nitrophenol phosphatase activity and 70% more β‐glucosidase activity in shoot homogenates than was found in P‐sufficient plants. Changes in both enzyme activities may be involved in the mobilization of plant resources during the early stages of P‐deficient growth. 相似文献
36.
Weiss DJ Lulich J 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1999,28(2):59-63
A dog with myelodysplastic syndrome had microcytic hypochromic anemia, with siderocytes, poikilocytosis, hypersegmented neutrophils and giant platelets in peripheral blood. In bone marrow, the erythroid series showed immaturity, asynchronous maturation and sideroblasts. Dysgranulopoiesis and dysthrombopoiesis also were present, and hemosiderin was increased. Serum iron concentration was high, and both iron deficiency and lead toxicity were excluded as the cause of dyscrasia. This case represents a unique variant of myelodysplastic syndrome, best described as sideroblastic myelodysplasia. We propose the terms dyserythropoiesis, sideroblastic dyserythropoiesis, dyserythropoiesis with excess blasts, myelodysplasia and sideroblastic myelodysplasia to describe and categorize myelodysplastic syndromes in dogs. 相似文献
37.
38.
The bovine neutrophil: Structure and function in blood and milk 总被引:1,自引:0,他引:1
Migration of polymorphonuclear neutrophil leukocytes (PMN) into the mammary gland provide the first line of defense against invading mastitis pathogens. Bacteria release potent toxins that activate white blood cells and epithelial cells in the mammary gland to secrete cytokines that recruit PMN that function as phagocytes at the site of infection. While freshly migrated PMN are active phagocytes, continued exposure of PMN to inhibitory factors in milk such as fat globules and casein, leads to altered PMN morphology and reduced phagocytosis. In the course of phagocytosing and destroying invading pathogens, PMN release chemicals that not only kill the pathogens but that also cause injury to the delicate lining of the mammary gland. This will result in permanent scarring and reduced numbers of milk secretory cells. The life span of PMN is limited by the onset of apoptosis. To minimize damage to mammary tissue, PMN undergo a specialized process of programmed cell death known as apoptosis. Macrophages quickly engulf and phagocytose apoptotic PMN, thereby minimizing the release of PMN granular contents that are damaging to tissue. The PMN possess an array of cell surface receptors that allow them to adhere and migrate through endothelium and to recognize and phagocytose bacteria. One receptor found on phagocytes that is receiving considerable attention in the control of infections by Gram-negative bacteria is CD14. Binding of lipopolysaccharide (LPS) to membrane bound CD14 causes release of tumor necrosis factor-alpha and sepsis. Binding of LPS to soluble CD14 shed from CD14-bearing cells results in neutralization of LPS and rapid recruitment of PMN to the site of infection. Recent advances in the fields of genomics and proteomics should greatly enhance our understanding of the PMN role in controlling intramammary infections in ruminants. Further, manipulation of PMN, through either recombinant proteins such as soluble CD14 that enhance PMN response or agents that mediate PMN apoptosis, may serve as novel therapeutics for the treatment of mastitis. 相似文献
39.
Aldrich JR Bartelt RJ Dickens JC Knight AL Light DM Tumlinson JH 《Pest management science》2003,59(6-7):777-787
This multi-author paper reviews current work by USDA-ARS scientists in the field of chemical ecology. Work with pheromones, the discovery and development of the codling moth kairomone, studies on insect-plant interactions and chemically mediated tritrophic plant-insect interactions have led to practical methods for control of important insect pests. 相似文献
40.
OBJECTIVE: To determine the functionality of canine anti-Malassezia IgE via the passive transfer of immediate hypersensitivity localized to the skin (ie, cutaneous anaphylaxis) from atopic dogs with dermatitis attributable to overgrowth of Malassezia pachydermatis (Malassezia dermatitis [MD]) to healthy recipient dogs by use of the Prausnitz-Küstner (P-K) technique. ANIMALS: 7 clinically normal dogs, 32 atopic dogs with MD, serum from 11 atopic dogs with MD, and 3 healthy dogs without prior sensitization to M pachydermatis. PROCEDURE: Serum from atopic dogs with MD was used for P-K tests in 3 clinically normal recipient dogs. Serial dilutions of untreated, heat-inactivated, IgE-absorbed, and bovine serum albumin (BSA)-absorbed (control) aliquots of serum were injected ID in triplicate for dermal sensitization. Twenty-four, 48, and 72 hours later, a crude extract of M pachydermatis was injected ID into the sites used for sensitization injections, and immediate hypersensitivity reactions were graded on a 4-point scale. RESULTS: Untreated serum caused P-K reactivity beginning 24 hours after passive sensitization and persisting through 72 hours (titers, 1:32 to 1:64). Heat inactivation and IgE-absorption of serum eliminated P-K reactivity, whereas treatment of serum with BSA did not. CONCLUSIONS AND CLINICAL RELEVANCE: Analysis of P-K test results supports the passive transfer of cutaneous anaphylaxis by anti-Malassezia IgE and indicates it is functional in type-1 hypersensitivity reactions of atopic dogs with MD. Reduction or blockade of anti-Malassezia IgE in atopic dogs with MD may provide better clinical control of the disease. 相似文献