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191.
This study established a prediction model for bending properties of glued-laminated timber (glulam) using optimized knot and
modulus of elasticity (MOE) distributions of lumber laminate as the main input variables. For this purpose, knot and MOE data
were investigated for all pieces of lumber that were prepared for glulam manufacturing, and statistical distributions of knot
size, knot number in one lumber, and MOE of each laminate were optimized as distribution functions. These knot and MOE data
were used as input variables in the prediction model for bending properties, and were also used in generating virtual glulam
using the inverse transform method. Prediction of bending properties for glulam was carried out using the transformed section
method, which is partially provided in ASTM D 3737 (Annex A4). Predicted values were compared with those from full-scale four-point
bending tests for 60 six-layered glulams with 10 different laminar combinations. Finally, the allowable bending properties
of glulam for each specific laminate combination were determined by calculating the fifth percentile of the modulus of rupture
and the average modulus of elasticity from virtual test results of more than 1000 virtual glulams. From the results of this
study, predicted bending properties for glulam and their distributions could be used for structural design in both allowable
stress design and limit state design. 相似文献
192.
There are several strategies that can be applied in SNP discovery as for example the locus-specific amplification of target genome regions 《分子植物育种》2007,5(2):248-249
There are several strategies that can be applied in SNP discovery, as for example the locus-specific amplification of target genome regions (Primmer et al., 2002; Van et al., 2004) or simultaneous assembly of anonymous sequences which are the product of whole genome shotgun sequencing (Webber and Myers, 1997) or reduced representation shotgun sequencing (Altshuler et al., 2000). The majority of these methods are highly cost-prohibitive, however, and there is a necessity for development of new strategies that would be more suitable for small and medium-scale laboratories. 相似文献
193.
Summary Interspecific hybrids have been obtained in an incompatible cross between Glycine max and G. tomentella through the in vitro culture of hybrid embryos. The percentage of successful pod setting in the crosses averaged 12.8% but there were marked differences depending on the soybean cultivar used as the female parent. Hybrid embryos at globular to heart stages were extracted from the embryo sac 15–25 days after pollination and cultured in vitro. Hybrid plants were successfully obtained by culturing the embryos on B5 medium supplemented with 0.1 mg/l IBA followed by culture on B5 medium supplemented with 0.1 mg/l TBA plus 0.25 mg/l 2-iP. The F1 plants resembled the wild male parent in growth form, but had an intermediate leaf shape between that of the parents. 相似文献
194.
Karyotype of Canada wildrye (Elymus canadenisis L.) was described using giemsa C-banding techniques. Most of the chromosomes showed dome banding pattern polymorphism. Small to large terminal and centormere bands were observed in most of the chromosomes. A faint satellite was observed in one chromosome. Tow chromosomes had a large interstitial band near the centromeres in the long arms. The Giemsa C-banding pattern of E. Canadensis is compared to that of Pseudoroegneria spicata and Critesion boddanii to illustrate species relationship. 相似文献
195.
196.
Cha KH Lee JY Song DG Kim SM Lee DU Jeon JY Pan CH 《Journal of agricultural and food chemistry》2011,59(16):8670-8674
Chlorella is a nutrient-rich microalga that contains protein, lipid, minerals, vitamins, and high levels of lutein. This study evaluated the bioavailability of lutein from Chlorella vulgaris using a coupled in vitro digestion and human intestinal Caco-2 cell model. Lutein bioaccessibility was low, and approximately 75% of total C. vulgaris lutein was not micellized during the digestion process but remained in the insoluble digestate. Microfluidization improved lutein micellization efficiency during C. vulgaris digestion. C. vulgaris was microfluidized at a pressure exceeding 10000 psi, and the cell surface disruption was visualized by scanning electron microscopy. The mean C. vulgaris particle size was reduced from 3.56 to 0.35 μm with the microfluidization treatment. C. vulgaris microfluidization at 20000 psi was three times more efficient for aqueous lutein micelles production as compared with untreated C. vulgaris, and the final lutein content accumulated by intestinal Caco-2 cells was also higher with microfluidization. C. vulgaris lutein stability was not affected by microfluidization. These results indicate that microfluidization may be useful for improving lutein bioaccessibility from C. vulgaris during food processing. 相似文献
197.
Kim SK Kim YC Lee S Kim JC Yun MY Kim IS 《Journal of agricultural and food chemistry》2011,59(3):934-938
Microorganisms capable of growth on oils are potential sources of biopesticides, as they produce complex molecules such as biosurfactants and lipopeptides. These molecules have antimicrobial activity against plant pathogens, but few data are available on their insecticidal activity. The present study describes the insecticidal activity of a rhamnolipid isolated from diesel oil-degrading Pseudomonas sp. EP-3 (EP-3). The treatment of cell-free supernatants of EP-3 grown on glucose-mineral medium for 96 h led to > 80% mortality of aphids (Myzus persicae) within 24 h. Bioassay-guided chromatography coupled with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MADLDI-TOF MS) and (1H, 13C) nuclear magnetic resonance (NMR) analyses was employed to isolate and identify the EP-3 insecticidal metabolites. Dirhamnolipid, with molecular formulas of C??H??O?? and C??H??O??, was identified as a main metabolite exhibiting insecticidal activity against aphids. Dirhamnolipid showed a dose-dependent mortality against aphids, producing about 50% mortality at 40 μg/mL and 100% mortality at 100 μg/mL. Microscopy analyses of aphids treated with dirhamnolipid revealed that dirhamnolipid caused insect death by affecting cuticle membranes. This is the first report of rhamnolipid as an insecticidal metabolite against M. persicae. Rhamnolipid shows potential for use as a pesticide to control agricultural pests. 相似文献
198.
199.
Methylation methods for the quantitative analysis of conjugated linoleic acid (CLA) isomers in various lipid samples 总被引:8,自引:0,他引:8
Park SJ Park CW Kim SJ Kim JK Kim YR Park KA Kim JO Ha YL 《Journal of agricultural and food chemistry》2002,50(5):989-996
Precise methylation methods for various chemical forms of conjugated linoleic acid (CLA), which minimize the formation of t,t isomers and allylmethoxy derivatives (AMD) with the completion of methylation, were developed using a 50 mg lipid sample, 3 mL of 1.0 N H(2)SO(4)/methanol, and/or 3 mL of 20% tetramethylguanidine (TMG)/methanol solution(s). Free CLA (FCLA) was methylated with 1.0 N H(2)SO(4)/methanol (55 degrees C, 5 min). CLA esterified in safflower oil (CLA-SO) was methylated with 20% TMG/methanol (100 degrees C, 5 min), whereas CLA esterified in phospholipid (CLA-PL) was methylated with 20% TMG/methanol (100 degrees C, 10 min), followed by an additional reaction with 1.0 N H(2)SO(4)/methanol (55 degrees C, 5 min). Similarly, CLA esterified in egg yolk lipid (CLA-EYL) was methylated by base hydrolysis, followed by reaction with 1.0 N H(2)SO(4)/methanol (55 degrees C, 5 min). These results suggest that for the quantitative analysis of CLA in lipid samples by GC, proper methylation methods should be chosen on the basis of the chemical forms of CLA in samples. 相似文献
200.
Jeong O-Young Park Hyeon-Su Baek Man-Kee Kim Woo-Jae Lee Geon-Mi Lee Chang-Min Bombay Maurene Ancheta Melanie Banzon Lee Jeom-Ho 《Journal of Crop Science and Biotechnology》2021,24(1):1-11
Journal of Crop Science and Biotechnology - Rice has been grown as a staple food in South Korea for several millennia. However, its status has not stayed constant as a result of changes in South... 相似文献