全文获取类型
收费全文 | 85023篇 |
免费 | 5240篇 |
国内免费 | 773篇 |
专业分类
林业 | 3299篇 |
农学 | 2575篇 |
基础科学 | 698篇 |
10308篇 | |
综合类 | 16909篇 |
农作物 | 3191篇 |
水产渔业 | 4040篇 |
畜牧兽医 | 43734篇 |
园艺 | 1191篇 |
植物保护 | 5091篇 |
出版年
2021年 | 755篇 |
2020年 | 835篇 |
2019年 | 1015篇 |
2018年 | 1377篇 |
2017年 | 1596篇 |
2016年 | 1426篇 |
2015年 | 1383篇 |
2014年 | 1574篇 |
2013年 | 3147篇 |
2012年 | 2956篇 |
2011年 | 3571篇 |
2010年 | 2373篇 |
2009年 | 2236篇 |
2008年 | 3204篇 |
2007年 | 3088篇 |
2006年 | 2780篇 |
2005年 | 2679篇 |
2004年 | 2434篇 |
2003年 | 2473篇 |
2002年 | 2338篇 |
2001年 | 2549篇 |
2000年 | 2587篇 |
1999年 | 1982篇 |
1998年 | 836篇 |
1997年 | 730篇 |
1995年 | 786篇 |
1994年 | 712篇 |
1993年 | 702篇 |
1992年 | 1583篇 |
1991年 | 1761篇 |
1990年 | 1685篇 |
1989年 | 1621篇 |
1988年 | 1511篇 |
1987年 | 1558篇 |
1986年 | 1625篇 |
1985年 | 1431篇 |
1984年 | 1216篇 |
1983年 | 1051篇 |
1982年 | 696篇 |
1979年 | 1087篇 |
1978年 | 867篇 |
1976年 | 743篇 |
1975年 | 773篇 |
1974年 | 1032篇 |
1973年 | 1010篇 |
1972年 | 964篇 |
1971年 | 898篇 |
1970年 | 869篇 |
1969年 | 829篇 |
1967年 | 715篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
961.
962.
Kara L. Moreno Elizabeth M. Scallan Beatriz P. Monteiro Paulo V. Steagall Bradley T. Simon 《Veterinary anaesthesia and analgesia》2021,48(4):570-576
ObjectiveTo evaluate the thermal antinociceptive effects of a high-concentration formulation of buprenorphine alone or followed by hydromorphone in conscious cats.Study designRandomized, blinded, placebo-controlled crossover study design.AnimalsA total of six purpose-bred, adult female ovariohysterectomized Domestic Short Hair cats.MethodsCats were allocated into three treatments each consisting of two injections, subcutaneous then intravenous (IV) administration, 2 hours apart: treatment SS, two injections of 0.9% saline; treatment BS, buprenorphine (0.24 mg kg–1, 1.8 mg mL–1) and saline; and treatment BH, buprenorphine (0.24 mg kg–1) and hydromorphone (0.1 mg kg–1). Skin temperature (ST) and thermal threshold (TT) were recorded before (baseline) and for 24 hours following first injection. TT data were analyzed using mixed linear models and a Benjamini–Hochberg sequential adjustment procedure (p < 0.05).ResultsThere were no significant differences among treatments for baseline ST and TT values, treatment SS over time and between treatments BS and BH. Compared with baseline, TT was significantly increased at all time points in treatments BH and BS except at 2 hours in treatment BS. TT was significantly higher than SS at 3–18 hours and 4–12 hours for treatments BS and BH, respectively. Maximal increases in TT were 47.5 °C at 2 hours, 53.9 °C at 3 hours and 52.4 °C at 6 hours in treatments SS, BS and BH, respectively.Conclusions and clinical relevanceAdministration of IV hydromorphone following high-concentration buprenorphine provided no additional antinociception and decreased the duration of effect when compared with high-concentration buprenorphine alone. Alternative analgesics should be considered if additional analgesia is required after administration of high-concentration buprenorphine. 相似文献
963.
Thirty calves (3 to 4 months old) were exposed conjunctivally to a pathogenic strain of Brucella abortus. Calves were euthanatized and necropsied at postexposure hours 2 and 4, and at postexposure days (PED) 1, 4, 7, 14, 21, 42, and 49. Selected ocular, pharyngeal, and lymphoid tissues were cultured bacteriologically for brucellae to determine organism distribution. Brucella abortus organisms initially localized in the third eyelids, bulbar conjunctivae, and parotid lymph nodes and were detected in these structures until PED 42, 21, and 49 respectively. In calves euthanatized at PED 7, organisms were in other cranial lymph nodes (mandibular and retropharyngeal), and in calves euthanatized at PED 21, organisms were isolated from peripheral lymphoid tissues. Brucellae were not isolated from mesenteric and bronchial lymph nodes and from the spleen until PED 21. The pattern of isolation indicated that conjunctival exposure probably resulted in entrance of brucellae into the host via ocular tissues. 相似文献
964.
Immune and bone properties of chicks consuming corn contaminated with a Fusarium that induces dyschondroplasia 总被引:1,自引:0,他引:1
A study was conducted to contrast the dyschondroplasia-inducing capability of several species of Fusarium with that of the natural fungi found in poultry diets and litter. Day-old broiler chicks were fed pure corn cultures of specific fungal isolates for 3 weeks. Humoral immunity to sheep red blood cells (SRBC), body weight, valgus and varus leg deformities, incidence of dyschondroplasia, and mechanical properties of the tibiotarsi were examined. F. equiseti #15 was the only fungal isolate to induce dyschondroplasia to any significant degree, which confirmed previous work in Minnesota. This isolate also suppressed growth rate and humoral immunity, although dyschondroplasia sometimes did occur without associated immunosuppression and growth depression. Bone strength of the tibiotarsal diaphysis was normal, and gross lesions of valgus and varus leg deformities did not appear to be related to the incidence of dyschondroplasia in the 3-week-old chicks. 相似文献
965.
S L Ihle R W Nelson J R Cook 《Journal of the American Veterinary Medical Association》1988,192(1):71-72
Hypercalcemia caused by primary hyperparathyroidism was believed to be responsible for seizures in a dog. A diagnostic evaluation showed no primary causes of seizures. After surgical excision of the adenomatous parathyroid gland, phenobarbital treatment was discontinued, without recurrence of seizures. 相似文献
966.
L Ferrer R M Rabanal M Domingo J A Ramos D Fondevila 《Research in veterinary science》1988,44(2):194-196
This paper describes the demonstration of Leishmania donovani amastigotes in canine tissues by immunoperoxidase staining. An indirect immunoperoxidase method was applied to the organs of 20 dogs in which leishmaniasis was previously diagnosed. Haemosiderin pigment was eliminated with 5 per cent oxalic acid. Amastigotes of L donovani appeared as dark brown stained bodies which contrasted with haematoxylin stained host cells. No positively stained amastigotes could be seen in any of the sections incubated with control serum. The organs which more frequently showed leishmanids were: skin (macrophages and fibroblasts), liver, spleen, lymph nodes and bone marrow. In a few cases amastigotes were seen in kidneys, gut, adrenal glands, eyes and testicles. This technique is simple to perform, gives consistent results and allows unequivocal histopathological diagnosis of canine leishmaniasis. 相似文献
967.
Immune exclusion of Haemonchus contortus larvae in sheep: dose dependency, steroid sensitivity and persistence of the response 总被引:2,自引:0,他引:2
F Jackson H R Miller G F Newlands S E Wright L A Hay 《Research in veterinary science》1988,44(3):320-323
Naive and immune sheep, treated with anthelmintic, challenged directly into the abomasum and killed 48 hours later were used to examine the steroid sensitivity, persistence and dose dependency of immune mechanisms which result in the failure of incoming Haemonchus contortus larvae to establish (immune exclusion). Immune exclusion was almost totally abolished by corticosteroid treatment, the mean percentage of larvae establishing in the mucosa of immune sheep was 1.4 per cent of the challenge infection whereas in those treated with dexamethasone it was 24.4 per cent and in naive sheep 27.3 per cent. Immune animals challenged after seven and 42 days without antigenic experience excluded more than 90 per cent of the challenge larvae from their mucosae, whereas those challenged after 84 days without antigenic experience were as susceptible as naive animals. Immune exclusion was dose dependent, animals challenged with 10(6) and 10(5) larvae excluded 93 per cent and 82.5 per cent of the challenge dose whereas those challenged with 10(4) larvae failed to exclude larvae. 相似文献
968.
An analysis of cellular proliferation, and synthesis of lymphokines and specific antibody in vitro by leucocytes from immunized cattle 总被引:1,自引:0,他引:1
The relationships between the production of lymphokines, cellular proliferation and antibody synthesis by immune bovine PBL in vitro was examined to identify the cellular reactions responsible for differences in the titres of serum antibodies in calves from selected sire lines and MHC Class I phenotypes. Leucocytes from 22 calves immunized with ovalbumin and DNP-BSA proliferated specifically in vitro in the presence of 1-10 micrograms/ml ovalbumin 7-28 days after the second vaccination. Significant correlations between the production of IL-2, IFN-gamma and maximum proliferation were observed for the total group. The quantity of specific antibody produced when PBL were incubated alone or with 10(-1)-10(-2) micrograms/ml ovalbumin was also correlated significantly with the maximum proliferation and the serum antibody titre between 7 and 14 days. Anti-ovalbumin IgG was also synthesized in MLRs where the quantity of antibody was significantly correlated with the magnitude of proliferation. The responses in vitro to DNP-BSA were too low to provide meaningful comparisons. The results indicate that at intervals during the period of increasing serum titres, events in the bovine antibody response in vivo can be replicated in vitro. In addition, assays for proliferation, IL-2 or gamma-IFN, or specific antibody can be used to assess the magnitude of the immune response in vivo in experimental groups of cattle. Significant sire line differences in the serological responses to ovalbumin were observed but DNP-BSA was a poorer antigen and differences in the responses to this antigen were not significant. However, the sire line differences in vivo were not reflected in vitro in proliferative and lymphokine assays; only the production of antibody in vitro was significantly correlated with the in vivo serum titre. 相似文献
969.
970.
DOUGLAS ALLEN Jr . dvm ms Diplomateacvs NATHANIEL A. WHITE II dvm ms Diplomateacvs DAVID E. TYLER dvm PhD Diplomateacvp 《Veterinary surgery : VS》1988,17(1):10-14
The morphologic effects of induced intraluminal hydrostatic pressures (IHPs) of 0, 9, and 18 cm H2 O were evaluated in 33 isolated equine jejunal segments. Fifteen segments were distended with Tyrode's solution for 1 hour and nine segments for 4 hours. Tyrode's solution was added as needed to maintain the prescribed pressures. Nine other segments were left undisturbed for 4 hours after the initial distention period. On decompression of the intestinal segments, progressive peristaltic contractions resumed in all segments. Evaluation of intestinal sections by light microscopy and transmission electron microscopy showed edema of the villi and submucosa and separation of the epithelial cells adjacent to the basement membrane in all segments. The epithelial cell necrosis found in ischemic intestine was not seen. This study indicates that the necrosis found at the villous tips in distended sections of small intestine remote from the site of obstruction cannot be reproduced by IHP increases of 4 hours duration. 相似文献