Inversion of TDR signals—revisited

In this study, we discuss the consistence of measured and calculated TDR traces. The calculated traces are solutions of a time domain reflectometry (TDR) forward solver, an algorithm for computing the TDR trace for a given dielectric profile along a transmission line. An unambiguous and efficient forward solver is a prerequisite for a good solution of the inverse problem, i.e., to extract the spatial distribution of the dielectric properties along the transmission line from a TDR trace. To advance our understanding of TDR inversion, we proceeded in two steps: (1) design of a TDR head section with minimal disturbances on the signal and (2) searching for causes why measured and predicted TDR traces differ. Based on a first experiment with a three‐rod TDR probe of 100 cm length, we demonstrated that our TDR forward solver—like others presented in literature—approximate the measured TDR traces apparently well but not precisely enough for signal inversion. In a second experiment, using a two‐rod TDR probe of 70 cm length, we addressed the problem of non‐parallel transmission lines. We found that the influence of a non‐parallel installation is similar to an increase of the electrical conductivity in soil water but can be distinguished from this property. A third experiment reveals that lateral and longitudinal disturbances in the vicinity of a TDR probe are of minor importance. From the analysis of our experiments, we found that neither lateral disturbances nor non‐parallel rods are responsible for the deviations between calculated and measured traces. This analysis showed us that structure in the sampled medium affects the shape of the TDR traces. Since minor deviations are essential for TDR‐signal inversion, we need new concepts to handle the fuzziness between measurements and calculations.     

Long-term development of soil organic carbon and nitrogen stocks after shelterwood- and clear-cutting in a mountain forest in the Bavarian Limestone Alps

Forest soils store large stocks of soil organic matter (SOM) and are of vital importance for the ecosystem supply with nutrients and water. According to the available literature, depending on management regime and site properties, different negative and positive effects of forest management (particularly of forest thinnings and shelterwood cuttings) on soil organic carbon (SOC) and nitrogen (N) stocks are observed. To elucidate the long-term impact of different shelterwood systems and small clear-cuttings on the OC and N stocks of shallow calcareous soils in the Bavarian Alps, we conducted soil humus inventories on different plots of a mixed mountain forest management experiment started in 1976. The silvicultural multi-treatment experiment consists of a NW-exposed Main Experiment (ME) site with eight plots of different cutting intensity (two unthinned controls, two light shelterwood cuttings = 30 % of basal tree area removed, two heavy shelterwood cuttings = 50 % removed, and two clear-cuttings = 100 % removed) on Triassic dolostone. Additionally, plots were installed at a N-exposed dolostone (ND) site and two sites (FL, FH) on Flysch sandstone (each with one unthinned control and one heavy shelterwood cutting). The shelterwood cuttings from 1976 were repeated in 2003 to re-establish the overstorey basal area as produced by the first cutting in the different plots. Thirty-five years after the first treatments, forest floor SOC and N stocks were significantly decreased (up to ?70 %) at the different shelterwood and clear-cut treatments compared to the unthinned control at the ME site despite vigorous development of natural rejuvenation. Also significantly smaller topsoil (forest floor plus mineral soil 0–10 cm depth) OC stocks (between ?16 and ?20 %) were detected at the thinned compared to the control plots. Differences in topsoil N stocks were also considerable (between ?3 and ?14 %), but substantially smaller than OC stock changes. For the total soil down to 30 cm depth, OC stocks in the differently thinned plots were consistently smaller compared to the unthinned control plots. Comparable to our findings at the ME site, heavy shelterwood plots at the three other sites (ND, FL, and FH) showed significant losses of OC in the forest floor (up to 43 %), mineral soil (up to 38 %), topsoil (up to 38 %), and total soil (up to 34 %). Significant large absolute and relative SOC decreases coincided with sites characterized by large initial humus stocks. Moreover, significant effects of heavy shelterwood cuttings on SOC and N stocks (on average 23 % SOC loss and 13 % soil N loss for the forest floor plus the uppermost 10 cm mineral soil) were detected on a regional level. Our results show that different shelterwood systems are accompanied with a considerable long-term decrease in OC and N stocks in shallow calcareous forest soils of the Bavarian Alps. However, a comparison with a windthrown forest stand at a nearby similar site indicates that SOM losses after thinning operations are small compared to decreases following windthrow or other calamities with subsequent large soil erosion and increased mineralization processes.     

3D-laser scanning: A non-destructive method for studying above- ground biomass and growth of juvenile trees

Many experiments with juvenile trees require the non-destructive monitoring of plant biomass and growth which is most often conducted with allometric relationships between easy to measure morphological traits and plant biomass. In a growth experiment with potted juvenile Fagus sylvatica L. trees, we tested the practicability and accuracy of the portable 3D-laser scanner ZF Imager 5006 using the phase difference method for measuring total above-ground biomass (stems, twigs, leaves), the biomass of axes (stems and twigs), of leaves biomass and the leaf area of 63 experimental trees. The trees were scanned from 20 (or 21) different positions with an angular step width of 0.036° in horizontal and vertical direction and the 3D-point cloud of every tree was translated into a point cloud grid with defined distances between the data points to standardise the spatial resolution of the data. The validation of the laser scan data against traditional biomass harvest data gave good correlations for total above-ground biomass (green and woody plant material combined), leaf biomass and leaf area (obtained by measurements before and after leaf harvest), and the mass of stems and twigs (only woody compartments of the plants) with R²-values between 0.61 and 0.88, all significant with p < 0.001. Biomass estimates using allometric regressions between total plant height or total leaf number and above-ground biomass as alternative non-destructive methods were found to be weaker than laser scanning (R² 0.54-0.67) and required a similar calibration effort. Repeated scanning of the same plant can be used to monitor biomass increase over time. We conclude that 3D-laser scanning is a promising technique for the non-destructive monitoring of biomass and growth in experiments with juvenile trees.     

Viability assessment of spermatozoa in large falcons (Falco spp.) using various staining protocols

Viability assessment is an important part of semen analysis, and various live/dead staining protocols have been used in semen of avian species. Results of live/dead count differed between dyes, staining protocols and bird species, impeding comparability between studies and requiring species-specific comparisons of viability stains. In raptor semen, similar comparisons are absent. Thus, the aim of the present study was to compare eight conventional viability stains. Eosin blue 2% [EB], eosin blue 2% with the addition of 3% sodium citrate [EB2], eosin blue–nigrosin 5% [EBN5], eosin yellow–nigrosin 5% [EYN5], eosin yellow–nigrosin 10% [EYN10], eosin blue–aniline blue [EBA], eosin yellow–aniline blue [EYA] and bromophenol blue–nigrosin [BBN] were evaluated in comparison with the fluorescence stain SYBR^® Green–propidium iodide [SYBR-PI] in spermatozoa of falcons. The comparison was performed using conventional light microscopy which is applicable in breeding centres, veterinary practices and field studies. Additionally, live/dead stains were correlated to motility values of the same samples to validate sperm viability. Light microscopy using EB and using SYBR-PI enabled an effective and clear differentiation between alive and dead spermatozoa of falcons. Motility values correlated significantly and strongly with EB only (r = .629; p < .001), but not with any other stain used in the study. Therefore, our results suggest EB as the most suitable stain for viability assessment in the semen of large falcons.     

Relationship between the forces acting on the horse's back and the movements of rider and horse while walking on a treadmill

Reasons for performing study: The exact relationship between the saddle pressure pattern during one stride cycle and the movements of horse and rider at the walk are poorly understood and have never been investigated in detail. Hypothesis: The movements of rider and horse account for the force distribution pattern under the saddle. Method: Vertical ground reaction forces (GRF), kinematics of horse and rider as well as saddle forces (FS) were measured synchronously in 7 high level dressage horses while being ridden on an instrumented treadmill at walk. Discrete values of the total saddle forces (FStot) were determined for each stride and related to kinematics and GRF. The pressure sensitive mat was divided into halves and sixths to assess the force distribution over the horse's back in more detail. Differences were tested using a one sample t test (P<0.05). Results: FStot of all the horses showed 3 peaks (P1‐P3) and 3 minima (M1‐M3) in each half‐cycle, which were systematically related to the footfall sequence of the walk. Looking at the halves of the mat, force curves were 50% phase‐shifted. The analysis of the FS of the 6 sections showed a clear association to the rider's and horse's movements. Conclusion: The saddle force distribution during an entire stride cycle has a distinct pattern although the force fluctuations of the FStot are small. The forces in the front thirds were clearly related to the movement of the front limbs, those in the mid part to the lateral flexion of the horse's spine and the loading of the hind part was mainly influenced by the axial rotation and lateral bending of the back. Potential relevance: These data can be used as a reference for comparing different types of saddle fit.     

Vitamin K2 is a mitochondrial electron carrier that rescues pink1 deficiency

Human UBIAD1 localizes to mitochondria and converts vitamin K(1) to vitamin K(2). Vitamin K(2) is best known as a cofactor in blood coagulation, but in bacteria it is a membrane-bound electron carrier. Whether vitamin K(2) exerts a similar carrier function in eukaryotic cells is unknown. We identified Drosophila UBIAD1/Heix as a modifier of pink1, a gene mutated in Parkinson's disease that affects mitochondrial function. We found that vitamin K(2) was necessary and sufficient to transfer electrons in Drosophila mitochondria. Heix mutants showed severe mitochondrial defects that were rescued by vitamin K(2), and, similar to ubiquinone, vitamin K(2) transferred electrons in Drosophila mitochondria, resulting in more efficient adenosine triphosphate (ATP) production. Thus, mitochondrial dysfunction was rescued by vitamin K(2) that serves as a mitochondrial electron carrier, helping to maintain normal ATP production.     

Cesium‐137‐based erosion‐rate determination of a steep mountainous region

Data on quantification of erosion rates in alpine grasslands remain scarce but are urgently needed to estimate soil degradation. We determined soil‐erosion rates based on ¹³⁷Cs in situ measurements. The method integrates soil erosion over the last 22 y (time after the Chernobyl accident). Measured erosion rates were compared with erosion rates modeled with the Universal Soil Loss Equation (USLE). The comparison was done in order to find out if the USLE is a useful tool for erosion prediction in steep mountainous grassland systems. Three different land‐use types were investigated: hayfields, pasture with dwarf shrubs, and pasture without dwarf shrubs. Our test plots are situated in the Urseren Valley (Central Switzerland) with a mean slope steepness of 37°. Mean annual soil‐erosion rates determined with ¹³⁷Cs of the investigated sites ranged between the minimum of 4.7 t ha^–1 y^–1 for pastures with dwarf shrubs to >30 t ha^–1 y^–1 at hayfields and pastures without dwarf shrubs. The determined erosion rates are 10 to 20 times higher compared to previous measurements in alpine regions. Our measurements integrated over the last 22 y, including extreme rainfall events as well as winter processes, whereas previous studies mostly reported erosion rates based on summer time and short‐term rainfall simulation experiments. These results lead to the assumption that heavy‐rainfall events as well as erosion processes during winter time and early spring do have a considerable influence on the high erosion amounts that were measured. The latter can be confirmed by photographs of damaged plots after snowmelt. Erosion rates based on the USLE are in the same order of magnitude compared to ¹³⁷Cs‐based results for the land‐use type “pasture with dwarf shrubs”. However, erosion amounts on hayfields and pasture without dwarf shrubs are underestimated by the USLE compared to ¹³⁷Cs‐based erosion rates. We assume that the underestimation is due to winter processes that cause soil erosion on sites without dwarf shrubs that is not considered by the USLE. Dwarf shrubs may possibly prevent from damage of soil erosion through winter processes. The USLE is not able to perform well on the affected sites. Thus, a first attempt was done to create an alpine factor for the USLE based on the measured data.     

Heart rate and hematological responses of Quarter Horses to a reining competition

The purpose of this study was to estimate the work intensity of a reining pattern and to determine hematological responses of trained Quarter Horses during a reining competition. Twelve Quarter Horses between four and eight years of age were equipped with a heart rate monitor at a National Reining Horse Association approved Horse Show. Heart rates were continuously recorded from approximately the last 30 minutes of the warm-up until two minutes after leaving the arena. The performance of each participant was video recorded and heart rates subsequently assigned to the different maneuvers. Blood samples were taken at rest and one minute after completion of the pattern. Average time needed to complete the pattern was 2:53±0:09 minutes over a distance of about 680 meters (3.9 m/s mean average velocity). The heart rate increased continuously from the beginning of the performance until a plateau was reached during canter. Highest heart rates were induced by roll-backs and stopping with 181±13 bpm. One minute after completion of the pattern heart rate dropped to 86±12 bpm. During the pattern mean plasma lactate increased significantly (P<0.001) to 5.1±1.9 mmol/l. PCV increased (p<0.05) to 48.6±2.8% with concomitant significant changes in the number of red blood cells (10.6±0.8×10¹²/1), hemoglobin concentration (17.5±1.2g/dl) and related parameters. The number of white blood cells increased significantly to 10.01±1.50×10⁹/1 with a shift in the numbers of neutrophils from 4.38±0.51×10⁹/1 to 5.53±0.87×10⁹/1 and in lymphocytes from 2.89±0.56×10⁹/1 to 3.96±0.86×10⁹/1.The reining pattern and its preceding warm-up period induced increased anaerobic glycolysis and subsequent mild accumulation of lactate. Performing a reining pattern represents only a submaximal workload with no signs of fatigue in adult Quarter Horses. Aerobic conditioning in combination with fast power training seems to be indicated in the training of reining Quarter Horses in addition to practicing motor skills.