全文获取类型
收费全文 | 504篇 |
免费 | 39篇 |
专业分类
林业 | 21篇 |
农学 | 17篇 |
基础科学 | 3篇 |
106篇 | |
综合类 | 31篇 |
农作物 | 25篇 |
水产渔业 | 34篇 |
畜牧兽医 | 268篇 |
园艺 | 6篇 |
植物保护 | 32篇 |
出版年
2024年 | 1篇 |
2023年 | 3篇 |
2022年 | 3篇 |
2021年 | 14篇 |
2020年 | 7篇 |
2019年 | 18篇 |
2018年 | 18篇 |
2017年 | 22篇 |
2016年 | 20篇 |
2015年 | 19篇 |
2014年 | 19篇 |
2013年 | 26篇 |
2012年 | 34篇 |
2011年 | 40篇 |
2010年 | 25篇 |
2009年 | 18篇 |
2008年 | 40篇 |
2007年 | 34篇 |
2006年 | 22篇 |
2005年 | 31篇 |
2004年 | 19篇 |
2003年 | 26篇 |
2002年 | 19篇 |
2001年 | 5篇 |
2000年 | 7篇 |
1999年 | 5篇 |
1998年 | 5篇 |
1997年 | 4篇 |
1996年 | 2篇 |
1995年 | 3篇 |
1994年 | 4篇 |
1992年 | 2篇 |
1991年 | 1篇 |
1990年 | 3篇 |
1988年 | 4篇 |
1987年 | 2篇 |
1986年 | 4篇 |
1985年 | 2篇 |
1983年 | 2篇 |
1982年 | 2篇 |
1981年 | 3篇 |
1979年 | 4篇 |
1975年 | 1篇 |
排序方式: 共有543条查询结果,搜索用时 15 毫秒
91.
Gaetano Laghetti Girolamo Fiorentino Karl Hammer Domenico Pignone 《Genetic Resources and Crop Evolution》2009,56(8):1163-1170
In 1970s it was thought that two species of ‘farro’, namely emmer (Triticum dicoccon Schrank) and einkorn (T. monococcum L.), were no longer present in Italy, but in 1981, some populations were discovered in a mountain area of the Appennino Sannita
(central southern Italy). In 2006/2007 three monitoring missions were carried out to check the current situation of these
ancient hulled wheats, specifically in the same areas where they were formerly collected. The same zones were also investigated
by both paleo-ethnobotany and agrobiodiversity points of view. The results of this research have shown that nowadays einkorn
is completely extinct while emmer is still cultivated in very few traditional farms, mainly as a fodder crop. A strong genetic
erosion was detected also for emmer and several nowadays samples were contaminated by seeds of modern spelt (T. spelta L.) varieties. In the present study the causes of this genetic erosion and which safeguarding actions to be undertaken are
discussed. 相似文献
92.
Real-time ultrasound imaging was used in a clinical study to estimate the number of follicles of different sizes, ovulation and conception rates, and to study follicle dynamics following oestrus-induction of bitches.
Follicles were identified during late anoestrus (between 100 and 60 days prior to the pre-ovulatory LH surge) and there appeared to be a shift in the population from small follicles (1–3 mm in diameter) to large follicles (>4 mm diameter) approximately 2 days prior to the LH surge. Corpora lutea could be reliably identified although the majority were cavitated. High ovulation rates (97–100%) and pregnant rates (86–100%) were detected, and although the conception rate was approximately 70% it varied between 8 and 92%. Within the narrow range of the clinical population studied there were trends relating age to reproduction performance. Oestrus induction with a gonadotrophin regime appeared to result in large numbers of small follicles that did not ovulate, whilst when using cabergoline the number of small and large follicels and the number of copora lutea were similar to those of control cycles. 相似文献
Follicles were identified during late anoestrus (between 100 and 60 days prior to the pre-ovulatory LH surge) and there appeared to be a shift in the population from small follicles (1–3 mm in diameter) to large follicles (>4 mm diameter) approximately 2 days prior to the LH surge. Corpora lutea could be reliably identified although the majority were cavitated. High ovulation rates (97–100%) and pregnant rates (86–100%) were detected, and although the conception rate was approximately 70% it varied between 8 and 92%. Within the narrow range of the clinical population studied there were trends relating age to reproduction performance. Oestrus induction with a gonadotrophin regime appeared to result in large numbers of small follicles that did not ovulate, whilst when using cabergoline the number of small and large follicels and the number of copora lutea were similar to those of control cycles. 相似文献
93.
M Russo M Vignoli G Catone F Rossi G Attanasi GCW England 《Reproduction in domestic animals》2009,44(S2):334-335
Ultrasonography has become the imaging modality of choice for evaluation of the prostate gland in the dog. Unfortunately, despite providing excellent images, it may be difficult to differentiate the common canine prostatic diseases with ultrasound because many have a similar ultrasonographic appearance. Real-time contrast-enhanced ultrasound was used to monitor and characterise the normal perfusion pattern and perfusion dynamics of the canine prostate gland when using a micro bubble contrast agent. In all contrast studies, the prostatic artery, entered the prostate gland on the dorso-lateral surface then tunnelled into the prostatic capsule and branched into many small parenchymal arteries which were directed medially towards the urethra to supply the body of the prostate gland. The flow of the contrast medium into the prostatic parenchyma was visible after 15 s. During the wash-in phase, there was an homogenous enhancement of the prostatic parenchyma. During the wash-out phase, an homogenous decrease of the echogenicity was visible in all cases. 相似文献
94.
Balmas V Delogu G Sposito S Rau D Migheli Q 《Journal of agricultural and food chemistry》2006,54(2):480-484
The methodology for the inclusion of tebuconazole (TBC) in beta-cyclodextrin (betaCD), spectroscopic characterization of the inclusion complex, and its activity for the control of a major soilborne disease of wheat caused by Fusarium culmorum are reported. Controlled release measured by chemical shift of the diagnostic protons H(3) and H(5) of betaCD confirmed stability of the complex at the solid state and in aqueous solution. Greenhouse and field experiments were conducted on durum wheat (Triticum durum cv. Prometeo) sown in substrate or in soil artificially infested with a virulent strain of F. culmorum. The inclusion complex betaCD-TBC, applied as seed dressing in combination or not with carboxymethylcellulose, reduced the disease incidence caused by F. culmorum and improved grain yield, showing effects that were generally comparable to those observed upon application of a commercial formulation of TBC. In the field experiment, only seed treatment with the inclusion complex betaCD-TBC allowed yield that was not different from that obtained from the uninoculated control. These results prove that by the use of the betaCD-TBC complex it is possible to obtain release of TBC and bioavailabilty of the fungicide without compromising its effectiveness. 相似文献
95.
Central core disease is a nonprogressive or slowly progressive congenital myopathy with a variable degree of hypotonia and axial and proximal muscle weakness that is histologically characterized by areas devoid of oxidative enzyme activity, resulting from an absence or low numbers of mitochondria in these regions (central core). A 10-month-old, male, pony foal was examined because of stiff gait, marked contractures of the distal portion of the limbs, flexion deformities of the hooves, and moderate hypotonia that had been present from birth. The foal had increased creatine kinase (282 U/liter; reference interval 10-135 U/liter), lactate dehydrogenase (1,188 U/liter; reference interval 150-450 U/liter), and aspartate transaminase (377 U/liter; reference interval <290 U/liter) activities, suggesting muscle disease. Muscle biopsy was performed. In cytochrome oxidase-, succinate dehydrogenase-, and reduced nicotinamide adenine dinucleotide tetrazolium reductase-reacted sections, the dominant morphologic feature was the absence of oxidative enzyme activity in the cores. By use of immunohistochemical technique with a monoclonal antibody against desmin, the cores were clearly delineated and a desmin network was present within the cores. Ultrastructurally, the core areas were characterized by preserved sarcomeres with irregular Z-lines, with some streaming or zigzag appearance and abnormal sarcoplasmic reticulum profiles and T-tubules. Lack of mitochrondria within central cores was observed. Diagnosis of myopathy with central cores was made. 相似文献
96.
Riccardo Aversano Salvatore Savarese Jose Maria De Nova Luigi Frusciante Maria Punzo Domenico Carputo 《Euphytica》2009,165(2):353-361
In this work we detected the extent of variability at nuclear and cytoplasmic DNA level of regenerated plants belonging to
Solanum genotypes with a different genetic background and somatic chromosome number. As for the nuclear characterization, a total
of 66 (18.5%) polymorphic bands were scored using 13 ISSR primers on 45 randomly selected regenerants. Our results show that
the regenerants obtained from clone cmm 1T and, at lower level, those from cph 1C are unstable under in vitro conditions or
rather more prone to in vitro-induced stress leading to somaclonal variation than the other genotypes used. Two types of changes
were observed: disappearance of parental ISSR fragments, termed “loss”; appearance of novel ISSR fragments, termed “gain”.
The most frequent event occurring in the regenerants was the loss of fragments (41 bands). Regenerated plants were analyzed
with seven plastid universal primers to determine the cytoplasmic composition at chloroplast level. All cpDNA primer pairs
tested produced amplicons of the same size in all genotypes analyzed and no polymorphic fragments were observed with any universal
primers used. Our results show that under in vitro culture conditions genotype affects the integrity of the genome. In addition,
the absence of polymorphism at plastid level confirms the greater genetic stability of cytoplasmic DNA. 相似文献
97.
Artificial spawning may precipitate high mortalities of red-tail black shark (Epalzeorhynchos bicolor) broodstock from Streptococcus iniae infection. Two vaccine formulations (aluminum or oil adjuvant) were evaluated for effectiveness. Fish (mean weight 21.2 ± 5.7 g) were harvested, acclimatized, and vaccinated by intracoelomic injection. After 21 days, fish were moved to a biosecure laboratory and, one day later, simultaneously spawned and challenged by bath or by intracoelomic injection with S. iniae. Both formulations increased relative% survival (RPS) in unspawned (RPS [formulation]: 80 [aluminum]; 54 [oil]) and spawned fish (87–95 [aluminum]; 73–75 [oil]), suggesting that vaccination increases survival of red-tail black shark broodstock after spawning. 相似文献
98.
99.
Use of Yunnan Baiyao and epsilon aminocaproic acid in dogs with right atrial masses and pericardial effusion 下载免费PDF全文
100.
Gianluca Neglia Bianca Gasparrini Domenico Vecchio Marcello Rubessa Rossella Di Palo Luigi Zicarelli Giuseppe Campanile 《Tropical animal health and production》2010,42(6):1243-1247
The aim of this study was to evaluate the effect of exogenous progesterone supplementation on superovulatory response in buffaloes
that has undergone a multiple ovulation program. Fourteen Mediterranean buffaloes were divided into two groups and received
a 4-day decreasing dosage of an equal mixture of 500 IU of FSH and LH starting on day 8 of the cycle. In group A (n = 7) a progesterone-releasing intravaginal device was removed on day 8, whereas in group B (n = 7) it was left till day 10, when PGF2a {\hbox{PG}}{{\hbox{F}}_{{2}\alpha }} was administered. Eighty hours later, buffaloes were artificially inseminated and after 6 days they undergone uterine flushing.
A higher (P < 0.05) number of corpora lutea (8.3 vs. 5.7) and embryo/flushing/buffalo (2.3 vs. 1.3) were recorded in group B vs. group
A if responsive buffaloes are considered (n = 12) and the number of corpora lutea was highly correlated with the number of embryos (r = 0.65; P < 0.05). In conclusion, progesterone supplementation during the first 2 days of the superovulation treatment seems to enhance
the recovery rate in buffalo species. A high ovulation rate, associated with a high number of corpora lutea, can represent
a parameter for estimating embryo recovery. 相似文献