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O Doi H Kusunoki T Sato S Kawakami T Fukuoka K Okuda O Ito E Saito T Hayashi T Hase M Kamiyosh 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(12):1361-1364
In 3 adult female cheetahs, induced-superovulation treatment was conducted, by means of 200 IU of pregnant mare serum gonadotropin (PMSG) and 100 IU of human chorionic gonadotropin (hCG) 80 hr after PMSG. The administration of PMSG created a sharp increase in the estradiol-17beta concentration, resulting in 232 pg/ml 8 hr later in one specimen out of three. The hCG administration showed an increase in the progesterone concentration of 2.29 ng/ml 46 hr later. In addition, after direct observation of the ovary surface by laparoscopy, 5 follicles in the right ovary over 2 mm in diameter, and 7 corpora lutea (5 in the right ovary and 2 in the left) were found. It is assumed that ovulation can be induced with hCG after 80 hr on PMSG during a cheetah's diestrus or proestrus. 相似文献
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We assessed stem burial and adventitious root formation of two late-successional species, Abies
mariesii and A. veitchii, in central Japan. In a plot (5 × 5 m), all seedlings between 8 and 24 cm tall were excavated: six A. mariesii seedlings in soil, and six and four A. veitchii seedlings in soil and on logs, respectively. For each sampled seedling, the number of terminal bud scars (TBS) was counted
on the aboveground and belowground stems. Stem length was measured, and divided into aboveground and belowground stems. Among
the three groups (A. mariesii seedlings in soil and A. veitchii seedlings in soil or on logs), there was no significant difference in height or total root weight (sum of adventitious roots
and primary roots), but diameter at ground level and number of TBS were significantly different. Counting TBS on the aboveground
stem of seedlings in soil underestimated seedling age, whereas the estimate was much closer to the true age for seedlings
on logs. Seedlings in soil formed more adventitious roots than seedlings on logs. A large proportion of the stem was buried
in humus for seedlings in soil, while most of the stem was not buried in humus for seedlings on logs. These results suggest
that substrate affects adventitious root formation, the formation of which is important to shade tolerance. Thus, our preliminary
results suggest that consideration of adventitious root formation is necessary to understand seedling bank dynamics and estimate
seedling ages of these Abies species in spatially heterogeneous old-growth subalpine forests. 相似文献
66.
Monotonic and reversed cyclic loading tests were conducted on dowel-type timber joints with varying degrees of wood decay
due to Fomitopsis palustris (Berk. et Curt.), a brown rot fungus, and the effect of decay on various shear performances of dowel-type joints was investigated.
For joints affected by the brown rot fungus, the initial stiffness, yield load, and maximum load of dowel-type joints were
significantly decreased, even with a small mass loss of wood. The reductions in shear performance were the largest for initial
stiffness, followed by yield load and maximum load, in that order. For a 1% reduction of the yield load, initial stiffness
and maximum load showed reductions of 1.15% and 0.77%, respectively. When dowel-type joints that had been exposed to the brown
rot fungus were subjected to reversed cyclic loading, the gap between the dowel and the lead hole of the wood was increased
and equivalent viscous damping was decreased. These results indicate that decay around the dowel lead hole especially affects
the load-displacement behavior at small displacement level, and dowel-type joints under cyclic loading have very low resistance
to forces acting on the wooden structure.
Part of this report was presented at the 5th Symposium on Timber Bridges of the Japan Society of Civil Engineers, Tokyo, July
2006; the 56th Annual Meeting of the Japan Wood Research Society, Akita, August 2006; and the Annual Meeting of the Architectural
Institute of Japan, Fukuoka, August 2007 相似文献
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Funakoshi Y Nakayama H Uetsuka K Nishimura R Sasaki N Doi K 《Veterinary pathology》2000,37(2):177-183
In canine mammary tumors, we examined the telomerase activity, proliferative activity by proliferative cell nuclear antigen (PCNA) immunohistochemistry, and percentage of apoptotic cells by the deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) method. The relationship between these measures and histopathologic malignancy was also investigated. PCNA index was highest in malignant tumors (adenocarcinoma: 27.0%; malignant mixed tumor: 15.7%), followed by benign tumors (adenoma: 4.4%; benign mixed tumor: 5.3%), hyperplasia (2.1%), and normal mammary gland (0.9%). In adenoma and adenocarcinoma, papillary and solid types showing higher cellularity tended to have higher PCNA indices than did cystic and tubular types. Although the TUNEL index was <1% in all cases, the relationship between this measure and histopathologic diagnosis showed the same tendency as observed in PCNA immunostaining. Telomerase activity was detectable in all adenomas, benign mixed tumors, and adenocarcinomas examined. In contrast, all normal mammary glands, hyperplasias, and malignant mixed tumors were negative for telomerase. Relative telomerase activity (RTA) of adenocarcinoma (56.5) was significantly higher than that of adenoma (27.8) and benign mixed tumor (33.9), and a significant positive correlation (P < 0.001) was noted between RTA and PCNA index. No significant correlations were noted between either PCNA or TUNEL index and clinical features such as metastasis and tumor diameter. PCNA index and telomerase activity may be useful markers for judging malignancy of canine mammary tumors. 相似文献
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Two cases of feline malignant craniopharyngioma 总被引:1,自引:0,他引:1
Nagata T Nakayama H Uchida K Uetsuka K Yasoshima A Yasunaga S Masuda K Tsujimoto H Kuwajima E Nishimura R Sasaki N Doi K 《Veterinary pathology》2005,42(5):663-665
Tumors at the cranial base in 2 cats (a 9 1/2-year-old, castrated male Chinchilla and a 7-year-old, castrated male American Shorthair) were diagnosed as malignant craniopharyngioma. The tumor lesion was histopathologically divided into four parts: 1) a small acinus part, in which relatively large cells with a pale cytoplasm composed small acini; 2) a duct part, in which small cuboidal cells composed ducts; 3) a cyst part, in which there were large cysts lined with flat cells; and 4) a pavement part, in which large multi-angular-shaped cells proliferated in a pavement pattern. The epithelial cells of some parts were positive for keratin by immunohistochemistry. Histopathologic findings of the present feline cases were identical to those of malignant craniopharyngioma in other animal species. 相似文献