甘肃省四种母猪繁殖障碍性疫病抗体血清学检测

运用乳胶凝集试验(LAT)和ELISA方法对酒泉、张掖、金昌、武威、兰州、临夏、白银、定西、天水和平凉10个市州38个规模化和农村个体养猪场送检的619份血样和血清进行了PPV、PR、PRRS和JE免疫抗体检测,3年度平均阳性率PPV为80.83%、PR为60.70%、PRRS为43.39%、JE为47.57%,免疫抗体平均阳性率以PPV最高,PRRS和JE免疫抗体平均阳性率偏低。对8市州24个猪场采送的132份血清进行的PPV、PR、PRRS和JE感染抗体检测表明,PPV感染抗体平均阳性率为18.57%、PR感染抗体平均阳性率为15.28%、PRRS感染抗体平均阳性率为23.28%J、E感染抗体平均阳性率为24.14%。     

Histological and immunohistochemical investigations of ovarian interstitial glands during non‐breeding season in camels (Camelus dromedarius)

The aim of this was to investigate the histology and immunohistochemistry of interstitial glands during non‐breeding season in camel ovaries. A total of 21 mature, non‐pregnant and apparently healthy camels aged between 8 and 12 years were slaughtered. The ovaries were removed within 15 min, cleaned from adipose tissue, weighted and examined grossly. The histological preparation was made, and then, the blocks were cut at 3–5 microns thickness and stained by H&E for histological examinations. Moreover, some sections were stained with Sudan Black for lipid detection. Immunohistochemical analysis of paraffin‐embedded ovarian tissues was performed to detect the localization of S‐100, vimentin, progesterone receptors (PR) and oestrogen receptors (ER). Immunoreactive signals were detected using UltraVision Detection System. The results revealed that the interstitial glands were located in the cortical region and they were arranged in various arrangements either single, in couple or in groups rich in lipid droplet. All interstitial gland arrangements were enclosed by connective tissue capsules containing fibroblasts and collagenous fibres separated them from the surrounding ovarian structures. Both interstitial glands and their surrounding CT were penetrated by several blood vessels. There was a strong immunoreactive signal for S‐100 in the nuclei of interstitial cells, and no signals were detected either in cells of the interstitial glands or their connective tissue with PR. We could conclude that the interstitial gland is distinct in ovary of camel and further studies are needed to elucidate its rule in steroid synthesis.     

大型精密仪器的管理与使用

介绍了农业分析室在大型精密仪器的管理与使用方面的具体做法。管理方面应注重简化体制，注重人才队伍建设，并建立相应的有偿使用制度；使用上则应努力做到教学、科研、生产相结合；并提出了常抓不懈、改变观念等几点有效管理大型精密仪器、充分发挥其功能的想法。只有科学地管理，才能充分发挥人、财、物的最佳效益。     

浑善达克沙地沙地榆土壤种子库特征与动态规律研究

在浑善达克沙地以沙地榆为研究对象,针对60年生、46年生、30年生3种不同树龄沙地榆林进行了土壤种子库调查,研究了沙地榆土壤种子库的组成、数量及动态变化规律。结果表明:1)不同树龄沙地榆林结实量差异明显,结实量大小顺序为60年生林分>46年生林分>30年生林分,结实量分别为2101粒/m2、451粒/m2、24粒/m2;2)在整个观测期内,不同树龄沙地榆林土壤种子库内种子总数呈下降趋势。2011年9月各林分种子总数比2011年6月分别减少了75.17%、47.46%和96.15%。3)从成熟种子组成及动态变化趋势来看,60年生和30年生沙地榆林土壤中成熟种子在整个观测期内呈逐月下降趋势,46年生沙地榆土壤中成熟种子总数呈先下降后增加的趋势。2011年7月,成熟种子的比例最小,种子质量最差,2011年9月,成熟种子的比例最大,不存在腐烂虫害种子,种子质量最好。4)不同树龄沙地榆林土壤种子库种子总数分布规律基本一致,表现为枯枝落叶层>0-2cm土层>2-4cm土层。枯枝落叶层土壤种子库种子总数随时间呈持续减少的趋势,0-2cm土层和2-4cm土层土壤种子库种子总数呈先减少后增加的趋势。     

聚合松香基环氧树脂的合成与表征

以聚合松香为原料,与环氧氯丙烷进行酯化反应、闭环反应,合成了聚合松香基环氧树脂,并对产物的红外光谱和核磁共振谱进行了解析.重点讨论了酯化反应、闭环反应影响因素对产物性能的影响.当适宜的反应条件为:聚合松香与环氧氯丙烷物质的量比约为1∶ 8,反应温度75℃,反应总时间8h,合成的环氧树脂的环氧值为0.19mol/100g,黏度(30℃)为30.8Pa · s,酸值为0.2mg/g.     

松香催化酯化反应研究进展

综述了30年来松香催化酯化反应的研究进展,重点考察了反应使用的催化剂、反应条件以及产品性能,对进一步研究松香酯化改性具有指导意义,并有利于我国松香资源的充分利用。     

Transmission studies of Hendra virus (equine morbilli-virus) in fruit bats, horses and cats

Objective To determine the infectivity and transmissibility of Hendra virus (HeV). Design A disease transmission study using fruit bats, horses and cats. Procedure Eight grey-headed fruit bats (Pteropus poliocephalus) were inoculated and housed in contact with three uninfected bats and two uninfected horses. In a second exper iment, four horses were inoculated by subcutaneous injection and intranasal inoculation and housed in contact with three uninfected horses and six uninfected cats. In a third experi ment, 12 cats were inoculated and housed in contact with three uninfected horses. Two surviving horses were inoculated at the conclusion of the third experiment: the first orally and the second by nasal swabbing. All animals were necropsied and examined by gross and microscopic pathological methods, immunoperoxidase to detect viral antigen in formalin-fixed tissues, virus isolation was attempted on tissues and SNT and ELISA methods were used to detect HeV-specific antibody. Results Clinical disease was not observed in the fruit bats, although six of eight inoculated bats developed antibody against HeV, and two of six developed vascular lesions which contained viral antigen. The in-contact bats and horses did not seroconvert. Three of four horses that were inoculated devel oped acute disease, but in-contact horses and cats were not infected. In the third experiment, one of three in-contact horses contracted disease. At the time of necropsy, high titres of HeV were detected in the kidneys of six acutely infected horses, in the urine of four horses and the mouth of two, but not in the nasal cavities or tracheas. Conclusions Grey-headed fruit bats seroconvert and develop subclinical disease when inoculated with HeV. Horses can be infected by oronasal routes and can excrete HeV in urine and saliva. It is possible to transmit HeV from cats to horses. Transmission from P poliocephalus t o horses could not be proven and neither could transmission from horses to horses or horses to cats. Under the experimental conditions of the study the virus is not highly contagious.