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排序方式: 共有239条查询结果,搜索用时 15 毫秒
191.
MARTIN A. VIDAL BVSc MS Diplomate ACVS SANDRA O. ROBINSON BS MANDI J. LOPEZ DVM MS PhD Diplomate ACVS DANIEL B. PAULSEN DVM PhD Diplomate ACVP OLGA BORKHSENIOUS PhD JILL R. JOHNSON DVM MS Diplomate ACVIM & ABVP RUSTIN M. MOORE DVM PhD Diplomate ACVS JEFFREY M. GIMBLE MD PhD 《Veterinary surgery : VS》2008,37(8):713-724
Objective— To compare the chondrogenic potential of adult equine mesenchymal stem cells derived from bone marrow (MSCs) or adipose tissue (ASCs). Study Design— In vitro experimental study. Animals— Adult Thoroughbred horses (n=11). Methods— BM (5 horses; mean [±SD] age, 4±1.4 years) or adipose tissue (6 horses; mean age, 3.5±1.1 years) samples were obtained. Cryopreserved MSCs and ASCs were used for pellet cultures in stromal medium (C) or induced into chondrogenesis±transforming growth factor‐3 (TGFβ3) and bone morphogenic factor‐6 (BMP‐6). Pellets harvested after 3, 7, 14, and 21 days were examined for cross‐sectional size and tissue composition (hematoxylin and eosin), glycosaminoglycan (GAG) staining (Alcian blue), collagen type II immunohistochemistry, and by transmission electron microscopy. Pellet GAG and total DNA content were measured using dimethylmethylene blue and Hoechst DNA assays. Results— Collagen type II synthesis was predominantly observed in MSC pellets from Day 7 onward. Unlike ASC cultures, MSC pellets had hyaline‐like matrix by Day 14. GAG deposition occurred earlier in MSC cultures compared with ASC cultures and growth factors enhanced both MSC GAG concentrations (P<.0001) and MSC pellet size (P<.004) after 2 weeks in culture. Conclusion— Equine MSCs have superior chondrogenic potential compared with ASCs and the equine ASC growth factor response suggests possible differences compared with other species. Clinical Relevance— Elucidation of equine ASC and MSC receptor profiles will enhance the use of these cells in regenerative cartilage repair. 相似文献
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JANE E. QUANDT DVM MARC R. RAFFE DVM MS Dipiomate ACVA ACVECC DAVID POLZIN DVM PhD Dipiomate ACVIM ELAINE P. ROBINSON BVetMed MVSc Dipiomate ACVA RONALD E. MANDSAGER DVM 《Veterinary surgery : VS》1991,20(5):357-361
Blood gas values were compared in blood collected from cut toenails and femoral arteries in 50 healthy crossbred dogs that were sedated and allowed to breathe room air spontaneously. Blood samples from cut toenails were collected by microcapillary technique with Natelson tubes. Femoral artery samples were collected by arterial puncture. Blood values for PO2, PCO2, pH, and HCO3 were compared. There was good correlation for pH, PCO2, and bicarbonate, but not for PO2. Microcapillary samples should be collected in 10 seconds or less for the most accurate results. A metal mixing "flea" was unnecessary. When properly handled, the Natelson tube technique provides an alternative method for collection of blood gas samples. 相似文献
194.
MARY A. McLOUGHLIN DVM MS RICHARD WALSHAW BVMS Dipiomate ACVS MICHAEL W. THOMAS DVM MS ROBERT W. DUNSTAN DVM MS 《Veterinary surgery : VS》1992,21(1):25-32
The urinary bladder of 10 clinically normal dogs was excised and the ureters were implanted into an isolated, vagotomized gastric segment derived from the fundic region of the stomach. The gastric segment was closed to form a conduit. Continence was maintained with a modified Kock "nipple valve" created from an isolated segment of ileum. Four dogs were euthanatized by day 30 because of complications related to the early onset of renal failure and electrolyte alterations. Six dogs were euthanatized on day 150. Ureteral dilatation, hydronephrosis, and decreased endogenous creatinine clearance rates were measured in all dogs at the end of the survival period. Pyelonephritis was diagnosed histologically in seven dogs. Positive renal cultures were obtained from seven dogs at necropsy. It was concluded that gastric conduit urinary diversion is unsatisfactory for long-term or short-term clinical use in dogs. 相似文献
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Marco Demaria DVM Bryden J. Stanley BVMS MVetSc Diplomate ACVS Joe G. Hauptman DVM MS Diplomate ACVS Barbara A. Steficek DVM PhD Diplomate ACVP Michele C. Fritz BSc LVT John M. Ryan MVB MRCVS Nathaniel A. Lam DVM Trevor W. Moore DVM Heather S. Hadley DVM 《Veterinary surgery : VS》2011,40(6):658-669
Objective: To compare the effect of negative pressure wound therapy (NPWT) with standard‐of‐care management on healing of acute open wounds in dogs. Study Design: Prospective, controlled, experimental study. Animals: Adult dogs (n=10). Methods: Full‐thickness 4 cm × 2 cm wounds were surgically created on each antebrachium and in each dog were randomized to receive either NPWT or standard wound dressings (CON) for 21 days. Dressing changes and wound evaluations were made at 8 time points. First appearance of granulation tissue, smoothness of granulation tissue, exuberance, percent epithelialization, and percent contraction were compared. Biopsies for histopathology were taken, and histologic scores determined, at 5 time points, and aerobic bacterial wound cultures performed at 2 time points. Results: Granulation tissue appeared significantly earlier, and was smoother and less exuberant in NPWT wounds compared with CON wounds. Percent contraction in NPWT wounds was less than CON wounds after Day 7. Percent epithelialization in NPWT wounds was less than CON wounds on Days 11, 16, 18, and 21. Histologic scores for acute inflammation were higher in NPWT on Day 3, and lower on Day 7, than CON wounds. Bacterial load was higher in NPWT on Day 7. Conclusion: NPWT accelerated appearance of smooth, nonexuberant granulation tissue; however, prolonged use of NPWT impaired wound contraction and epithelialization. 相似文献
197.
LOUISE L. SOUTHWOOD BVSc PhD Diplomate ACVS Diplomate ACVECC JENNIFER COHEN VMD EVITA BUSSCHERS DVM PERRY HABECKER VMD Diplomate ACVP 《Veterinary surgery : VS》2010,39(1):101-106
Objective— To report a case of multiple acquired jejunal pseudodiverticula managed successfully by resection and jejunojejunostomy.
Study Design— Clinical report.
Animals— Arabian filly.
Methods— The filly was referred for signs of acute colic of several hours duration and had a 2-week history of inappetence and weight loss. Three meters of thickened, edematous, and dilated jejunum removed during exploratory celiotomy had an intussusception and numerous diverticula; jejunojejunostomy performed. The pathoanatomic diagnosis was proliferative enteropathy with pseudodiverticula formation and jejunal muscular hypertrophy and diverticulosis. The diverticula appeared to be acquired; however, the exact cause was not determined.
Results— There were no postoperative gastrointestinal complications and 1 year later, the filly was doing well.
Conclusion— Multiple acquired jejunal pseudodiverticula not associated with classic muscular hypertrophy can occur in young horses. Long-term prognosis seemingly can be excellent after resection and jejunojejunostomy.
Clinical Relevance— Compared with other reports of small intestinal diverticula in horses, this case is unique because it was not congenital or associated with classic muscular hypertrophy. 相似文献
Study Design— Clinical report.
Animals— Arabian filly.
Methods— The filly was referred for signs of acute colic of several hours duration and had a 2-week history of inappetence and weight loss. Three meters of thickened, edematous, and dilated jejunum removed during exploratory celiotomy had an intussusception and numerous diverticula; jejunojejunostomy performed. The pathoanatomic diagnosis was proliferative enteropathy with pseudodiverticula formation and jejunal muscular hypertrophy and diverticulosis. The diverticula appeared to be acquired; however, the exact cause was not determined.
Results— There were no postoperative gastrointestinal complications and 1 year later, the filly was doing well.
Conclusion— Multiple acquired jejunal pseudodiverticula not associated with classic muscular hypertrophy can occur in young horses. Long-term prognosis seemingly can be excellent after resection and jejunojejunostomy.
Clinical Relevance— Compared with other reports of small intestinal diverticula in horses, this case is unique because it was not congenital or associated with classic muscular hypertrophy. 相似文献
198.
Fabien Relave DVM Diplomate ACVS Florent David DVM M Sc Diplomate ACVS & ECVS Mathilde Leclère DVM Diplomate ACVIM Kate Alexander DVM M Sc Diplomate ACVR Pierre Hélie DVM Diplomate ACVP Michael Meulyzer DVM Diplomate ACVS Jean Pierre Lavoie DVM Diplomate ACVIM Marcel Marcoux DVM M Sc 《Veterinary surgery : VS》2010,39(7):839-846
Objective: To validate the use of the LigaSure? Vessel Sealing System (LVSS) to perform thoracoscopic lung tissue biopsies in heaves‐affected horses. Study design: Prospective clinical study. Animals: Heaves‐affected horses (n=12). Methods: Lung biopsies (n=34) were collected with the LVSS (2–4 biopsies/horse) in horses with and without clinical signs of heaves. Thoracoscope (13th intercostal space [ICS]) and 2 instruments (between the 12–15th ICS) portals were used. Selected clinical and arterial blood gas variables were monitored. Postoperative pneumothorax was evaluated. Depth of thermal injury to the surrounding tissue and representativeness of the biopsies were determined. Results: Mean surgical time was 22.9±8.0 minutes. The complication rate was 5.6%, and primarily related to a focal inadequate sealing of the biopsy margin. Five horses in exacerbation required intraoperative intranasal O2. Mean PaO2 was significantly lower in heaves‐affected horses with clinical signs compared with those without clinical signs. Postoperative pneumothorax was detected radiographically after 20 of the 34 procedures. One horse with clinical signs of heaves developed a fatal tension pneumothorax 5 days postoperatively despite close radiographic monitoring. Conclusion: Thoracoscopic lung biopsy using LVSS is a rapid and effective technique to harvest peripheral lung tissues from heaves‐affected horses. Although the complication rate was tolerable, tension pneumothorax was a potential life‐threatening complication because of incomplete lung sealing. Clinical Relevance: LVSS can be used with relative safety to perform thoracoscopic lung biopsy, but close postoperative monitoring is necessary to avoid tension pneumothorax. 相似文献
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