Evidence from nucleic acid hybridization tests for geminivirus infection of ornamental crotons in India

Reduced growth of plants and excessive generalised and spotty yellowing of leaves occurred in ornamental crotons that were infected with a geminivirus, which was detected by spot hybridization tests using a cloned probe derived from DNA-A of acalypha yellow mosaic geminivirus. The croton virus was transmitted by the whitefly,Bemisia tabaci, toNicotiana tabacum andAcalypha indica, in which it caused leaf curl and yellow mosaic, respectively, but it was not transmitted to five other species. Infected crotons may therefore serve as reservoirs of a geminivirus that is able to infect a crop species.     

Prevalence of fenbendazole resistance in ovine nematodes in north west india

Summary A survey was undertaken between March and September 1992 to assess the prevalence of fenbendazole resistance in sheep nematodes in 32 traditionally managed rural flocks and 22 intensively managed flocks on 3 farms. Fenbendazole, a commonly used anthelmintic was used at the recommended dose of 5 mg/kg body weight. Efficacy was determined on the basis of percentage reduction in strongyle faecal egg counts (FECR%) and larval cultures before and between 7 and 10 days after treatment. None of the traditionally managed rural flocks showed resistance and all had greater than 99% reduction in faecal egg counts after treatment.Of the 22 intensively managed flocks, however, 15 had slight fenbendazole resistance (between 60 and 90% reduction in egg counts) and 4 had severe resistance (less than 60% reduction).

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Prevalencia De La Resistencia A Fenbendazole En Nematodos Ovinos En El Noroeste De India

Resumen Se llevó a cabo un estudio entre marzo y septiembre de 1992, para determinar la prevalencia de resistencia a fenbendazole de nemátodos ovinos en 32 rebaños con un manejo tradicional y 22 con un manejo intensivo en 3 fincas. El fenbendazole, un antihelmíntico comunmente usado, se dosificó a la dosis recomendada de 5 mg/kg de peso vivo. La eficacia fue determinada sobre la base del porcentaje de reducción en el conteo de huevos de estróngilus y los cultivos de larvas antes y entre 7 y 10 días después del tratamiento. Ninguno de los apriscos manejados tradicionalmente mostraron resistencia y todos tuvieron más del 99% de reducción en el conteo de huevos después del tratamiento.De los rebaños manejados intensivamente, 15 tuvieron una ligera resistencia al fenbendazole (entre 60 y 90% de reducción en el conteo de huevos) y 4 tuvieron resistencia marcada (menos de 60% de reducción).

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Prevalence De La Resistance Au Fenbendazole De Nematodes Chez Des Ovins Du Nord-Ouest De L'Inde

esumé Une enquête a été entreprise de mars à septembre 1992 pour évaluer la prévalence de la résistance au fenbendazole de nématodes dans 32 troupeaux de moutons conduits en élevage traditionnel et 22 en élevage intensif appartement à 3 fermes. Le fenbendazole, anthelminthique communément utilisé, a été administré à la dose recommandée de 5 mg/kg de poids vif. Son efficacité a été déterminée à partir de la réduction du pourcentage du nombre d'oeufs de strongles dans les fèces et à partir de cultures de larves avant et entres le 7e et 10e jours après le traitement. Aucun des troupeaux en élevage traditionnel n'a montré de résistance au médicament et tous ont présenté une réduction de 99 p.100 du nombre d'oeufs dans les féces après le traitement. Une légère résistance au produit (entre 60 et 90 p.100 de réduction du nombre d'oeufs) a cependant été démontrée dans 15 des 22 troupeaux en élevage intensif et une résistance grave (réduction de moins de 60 p.100) dans 4 autres.

     

Prevalence of antimicrobial resistance in fecal generic Escherichia coil isolated in western Canadian beef herds. Part II--cows and cow-calf pairs.

The objective of this study was to describe antimicrobial resistance in fecal generic Escherichia coli isolated from cows and cow-calf pairs from western Canadian beef herds. Susceptibility testing was completed on 1555 E. coli isolates (n = 533 cows from 69 herds) harvested from fresh fecal samples in the spring of 2002, and 630 isolates (n = 105 cow-calf pairs from 10 herds) collected in the spring of 2003. Only 1 cow isolate was resistant to an antimicrobial classified by Health Canada as being of very high importance to human medicine. Resistance to at least 2 antimicrobials was detected in 7.1% of the 2002 cow isolates, in 3.4% of the 2003 cow isolates, and 23.2% of the 2003 calf isolates. In the cows, resistance to at least 1 antimicrobial was not associated with cow breed (P = 0.16), cow age (P = 0.14), or previous cow treatment (P = 0.56). In the calves, resistance to at least 1 antimicrobial was not predicted by whether or not its dam was resistant to at least 1 antimicrobial (P = 0.36).     

Seasonal Dynamics of Metabolic Mechanisms Mediating Pyrethroid Resistance in Helicoverpa armigera in Central India

Very high cypermethrin and fenvalerate resistance frequencies were recorded in Helicoverpa armigera (Hübner) populations in central India during the 1993–94, 1994–95 and 1995–96 cropping seasons. Synergism assays and biochemical analyses of detoxification enzyme levels indicated that mono-oxygenases and esterases were important metabolic mechanisms mediating pyrethroid resistance. Piperonyl butoxide- (PBO) and profenofos-suppressible pyrethroid resistance were correlated with enhanced levels of cytochrome P450 and general esterases respectively. Enzyme assay data indicated that high cytochrome P450 levels generally coincided with low esterase activity and vice versa. Similarly, synergist bioassays showed that PBO-insensitive resistance was frequently associated with profenofos-sensitive resistance and vice versa. Oxidase- and esterase-mediated mechanisms evidently alternated in a reciprocal manner, with perceptible shifts in relative importance occurring during mid-October in all three seasons and in late January in 1995. Apart from metabolic mechanisms, a synergist-insensitive resistance mechanism (believed to be nerve insensitivity), accounted for an average of 51, 30 and 28% of cypermethrin resistance during the 1993–94, 1994–95 and 1995–96 seasons respectively. © 1997 SCI.     

Changes in the somatosensory evoked potentials and spontaneous electroencephalogram of hens during stunning in argon-induced anoxia

This study examined the time to loss of consciousness in hens during stunning in argon-induced anoxia. Somatosensory evoked potentials (SEPs) and the spontaneous electroencephalogram (EEG) were recorded in 12 culled hens prior to and during stunning in less than 2% oxygen (air displaced by argon). An additional 20 hens were stunned with a similar concentration of oxygen and the time to loss of posture, eye closure, and the onset and duration of clonic and tonic convulsions were recorded. A further 10 hens were immersed in less than 2% oxygen for 15-17 s and their response to comb pinching was tested as soon as they had been transferred to atmospheric air. It is concluded that the birds had not lost the primary response in their SEPs by the time they started convulsing, but the reduction in the amplitude of the SEPs, changes in their spontaneous EEG and a negative response to comb pinch before the start of the convulsions indicated that the birds were unconscious when they convulsed.     

Isolation and functional analysis of the zmARM4 locus in a novel maize (Zea mays) grain-filling mutant

Maize grain filling (GF) and grain weight (GW) are closely associated with grain yield. In our study, the ZmARM4 locus affecting GF and GW was identified using a reverse genetics screen of UniformMutator (Mu). First, MutatorDR-flanking sequence (MuDRFs) was isolated from the mutant by the MuDR-amplified fragment length polymorphism method, and it was verified by cosegregation (between MuDRFs and phenotype) and linkage analyses. Second, the target gene of the MuDRFs was found to encode the Armadillo domain of the repeat protein gene families and has high expression in the GF stage. Third, association mapping between SNPs in the ZmARM4 region and the GW of 489 maize lines indicated that ZmARM4 is a functional gene that is closely related to GW. Moreover, bioinformatics analysis suggested that ZmARM4 may be relevant for the activity of sucrose transport or the number of cell divisions in endosperm cells. Therefore, ZmARM4 may be used as an important locus for maize GW in molecular breeding.     

High efficacy of white spot syndrome virus replication in tissues of freshwater rice‐field crab,Paratelphusa hydrodomous (Herbst)

An attempt was made to determine the replication efficiency of white spot syndrome virus (WSSV) of shrimp in different organs of freshwater rice‐field crab, Paratelphusa hydrodomous (Herbst), using bioassay, PCR, RT‐PCR, ELISA, Western blot and real‐time PCR analyses, and also to use this crab instead of penaeid shrimp for the large‐scale production of WSSV. This crab was found to be highly susceptible to WSSV by intramuscular injection. PCR and Western blot analyses confirmed the systemic WSSV infection in freshwater crab. The RT‐PCR analysis revealed the expression of VP28 gene in different organs of infected crab. The indirect ELISA was used to quantify the VP28 protein in different organs of crab. It was found that there was a high concentration of VP28 protein in gill tissue, muscle, haemolymph and heart tissue. The copy number of WSSV in different organs of infected crab was quantified by real‐time PCR, and the results revealed a steady increase in copy number in different organs of infected crab during the course of infection. The viral inoculum prepared from different organs of infected crab caused significant mortality in tiger prawn, Penaeus monodon (Fabricius). The results revealed that this crab can be used as an alternate host for WSSV replication and production.