斜茎黄芪生物碱成分GC-MS分析

采用生物碱系统提取、薄层色谱和GC-MS分析技术,研究斜茎黄芪生物碱成分.结果表明,4.5 kg斜茎黄芪得到酸性和碱性氯仿部分生物碱分别为5.4g和3.6g,提取率为1.3％和0.8％;薄层色谱分析,酸性和碱性氯仿部分生物碱分别在氯仿∶甲醇=15∶1(Ⅴ∶ Ⅴ)和甲醇∶氨水=10∶1(Ⅴ∶Ⅴ)展开体系展开效果最佳,均呈现5个显色斑点;GC-MS分析,酸性和碱性氯仿部分各鉴定出7种生物碱,酸性氯仿部分以别隐品碱(34.84％)、原阿片碱(31.36％)和坎那定(21.46％)含量较高,碱性氯仿部分以别隐品碱(71.74％)、Oxyhydrostinine (7.45％)和坎那定(5.35％)含量较高.斜茎黄芪含有生物碱,且以别隐品碱、原阿片碱、坎那定碱和Oxyhydrostinine为主.     

母鼠围产期铅暴露对仔鼠组织微量元素含量的影响及乙酰半胱氨酸的保护效应

为观察母鼠围产期铅暴露对仔鼠各组织微量元素含量的影响及N-乙酰半胱氨酸(NAC)的保护效应,采用电感耦合等离子体质谱法(ICP-MS)测定低水平铅暴露后的仔鼠血液、大脑、骨骼、肝脏和肾脏等组织中的铅、锌、铜、铁、锰和硒等微量元素的含量。结果表明,与对照组相比,染铅组所测组织铅含量均极显著升高(P<0.01),锌含量均极显著降低(P<0.01);肝脏铁含量、肾脏硒含量及骨骼铁、铜、硒含量均显著降低(P<0.01或P<0.05)。NAC拮抗组与染毒组比较,各组织铅含量显著降低(P<0.01或P<0.05),血液和肾脏锌含量显著升高(P<0.05),其他元素含量无明显差异(P>0.05)。     

草原保护和建设是内蒙古长期坚持的绿色工程

总结了内蒙古 2 0年来草原建设和保护的经验、技术 ,探讨了发展的条件和对策 ,为面向2 1世纪农业技术的发展提供了理论依据和参考     

Analysis of the CpG Methylation Pattern on GDF11 Gene Exon 1 in Ujumqin Sheep

In order to investigate the methylation patterns on growth differentiation factor 11 (GDF11) gene exon 1 in Ujumqin sheep,we compared the level of methylation of GDF11 gene exon 1 in common Ujumqin sheep (CUS) and multi-vertebrae Ujumqin sheep (MUS) using the bisulfite sequencing PCR (BSP) method.The results showed that the average methylation rate of GDF11 gene exon 1 for MUS was extremely significantly higher than that for CUS (P<0.01),the average methylation rate of common Ujumqin sheep and multi-vertebrae Ujumqin sheep were 0.123 and 0.569.Analysis of 13 CpGs of GDF11 gene exon 1 showed that the CpG_11 and CpG_13 methylation sites had the highest methylation rate with a combined methylation value of 90% for MUS.Our results suggested that differential DNA methylation of GDF11 gene exon 1,in particular at these two sites,might cause the increase in the number of vertebrae in Ujumqin sheep.     

铅镉联合对新生大鼠大脑皮质神经细胞形态学损伤的研究

为了观察铅、镉及其联合对新生大鼠大脑皮质神经细胞的形态学影响,本试验通过对原代培养的新生大鼠大脑皮质神经细胞经提取、纯化和鉴定后,分九组进行单独或联合染毒,染毒时间为12 h,显微镜观察神经细胞形态并测量其胞体短直径和突起长度的变化.结果显示,与对照组相比,各试验组神经细胞密度降低,神经网络减少;各试验组神经细胞胞体短直径显著减小(P<0.05),突起长度显著变短(P<0.05);随铅、镉单剂量作用浓度的增加,神经细胞胞体短直径和突起长度有减小趋势,但组问差异不显著(P>0.05).与相应铅、镉单独染毒组比较.各铅镉联合染毒组神经细胞胞体短直径减小、突起长度变短更加明显,部分组间差异显著(P<0.05).结果表明,铅、镉作用浓度与神经细胞形态改变存在明显的剂量一效应关系,铅镉联合表现协同毒性效应.     

以蔗糖为碳源制备1,6-二磷酸果糖的研究

研究了以蔗糖为碳源,用啤酒酵母发酵生产1,6-二磷酸果糖(FDP)的最佳条件.其优化控制条件为:摇床转速150 r/min,发酵温度28℃,pH 7.0,培养7 h,蔗糖浓度0.08 g/mL、磷酸二氢钠浓度0.05 g/mL、甲苯浓度0.05 mL/mL、啤酒酵母∶水=1∶4时,FDP的积累量最高,达42.87 mg/mL.     

小麦秆锈菌新小种Ug99及其抗病育种研究进展(英文)

为帮助我国小麦研究人员全面了解秆锈菌新小种-Ug99 及其对我国小麦生产的潜在威胁,呼吁加强 Ug99 及其变异小种传播与扩散的监控,加强抗病种质创新和持久性耐病新品种选育,提前预防 Ug99 的危害。对 Ug99 小种变异、致病力、分布扩散以及小麦及其亲缘种属抗病基因发掘、连锁分子标记筛选,抗性育种策略与进展,中国 Ug99 研究现状进行了总结,由此不难看出,Ug99 是容易发生小种变异、毒力极强、扩展速度较快的秆锈菌新小种,极有可能对世界小麦生产带来的全球性危害。应该加强小麦及其亲缘种属新抗病基因的发掘与利用和持久性耐病新品种的培育,控制和预防 Ug99 及其变异小种的危害。     

Two Methods of Vitrification Followed by In Vitro Culture of the Ovine Ovary: Evaluation of the Follicular Development and Ovarian Extracellular Matrix

The aim of this study was to evaluate the influence of two vitrification techniques on the extra cellular matrix (ECM) and ovarian follicular development. The ovarian cortex was fragmented (9 mm³) and divided into six groups, viz. fresh control, cultured control, vitrified by the Ovarian Tissue Cryosystem (OTC) method, conventional solid surface vitrification (SSV) method, OTC/cultured and SSV/cultured. Follicles from all the fragments were analysed for morphology, development and viability. The ECM was evaluated based on the condition of collagen and reticular fibres and the immunolocalization of type I collagen and fibronectin. After 7 days of culture, the tissue vitrified by OTC revealed a higher percentage (p < 0.05) of morphologically normal (30.66%) and viable (60.00%) follicles when compared with those vitrified using the SSV technique (21.33% and 23.00%). In all the fragments cultured, regardless of the vitrification method, a significantly higher percentage of developing follicles was observed when compared with the non‐cultured tissue. Analysis of the type I collagen showed increased immunostaining after the in vitro culture in the vitrified fragments. In conclusion, the OTC is better for preserving the follicular viability and morphology and maintaining the integrity of the extracellular matrix components of the ovine ovary.