A case of acute pancreatic necrosis in the dog

The history, clinical signs and treatment by partial pancreatectomy of a dog with acute haemorrhagic pancreatic necrosis are described. Twelve days after surgery, a balance study indicated 95% fat assimilation; faecal trypsin levels ranged between 31 and 72 azo-casein units, and an oral glucose tolerance test indicated the presence of good insulin reserve and delayed stomach emptying.
Résumé . Les auteurs décrivent les antécédents, les symptmes cliniques et le traitement par pancréatectomie partielle d'un chien souffrant de nécrose hémorragique du pancréas. L'analyse des bilans effectuée douze jours après l'intervention a indiqué que les graisses étaient assimilées à 95 p. 100.
Les taux de trypsine des fèces se sont situés entre 31 and 72 unités d'azocaséine. Le test de la tolérance du glucose administré par voie buccale a révélé la présence d'une bonne réserve d'insuline et une décharge différée de l'estomac.
Zusammenfassung . Die Krankengeschichte, der klinische Befund und die Behandlung durch partielle Pancréatectomie eines Hundes mit akuter hämorrhagischer pankreatischer Nekrose werden beschrieben. Zwölf Tage nach dem chirurgischen Eingriff ergab eine Stoffwechseluntersuchung 95% Fettassimilierung; die fäkalen Trypsinkonzentrationen lagen zwischen 31 und 72 Azokaseineinheiten, une ein oraler Glukosetoleranztest ergab das Vorhandensein einer guten Insulinreserve und verzögerte Magenentleerung.     

Amplification of mesenchymal stem cells from human bone marrow and orientation to induce MSCs differentiating into endothelial cells in vitro

AIM: Our purpose was to induce MSCs differentiating into endothelial cells (EC) in vitro and to provide the seed cells for study of cardiovascular tissue-engineering. METHODS: MSCs were separated by gradient centrifugation on Percoll (density 1 073 g/L) from human bone marrow (HBM), and incubated for purification and amplification in DMEM (low glucose) with 10% fetal bovine serum (FBS). Then, the MSCs were incubated for orientation differentiated into EC in DMEM (high glucose) with 20% FBS, VEGF (10 μg/L), bFGF (5 μg/L), L-glutamine (2 mmol/L), penicillin (1×105U/L) and streptomycin (100 mg/L) for about 14-21 days and their phenotypic characteristics were analyzed by flow cytometry. Afterwards, the differentiating cells were evaluated by histology and immunohistochemistry. RESULTS: The quantity of MSCs was increased from 5.0×105 in the primary culture to 8.0×1012, or to increase to 1.6×107 times after 15 generations of incubation. The purity of MSCs was above 95% and 98% homogeneous at passages 2 and 3, respectively. About 80%-90% of the differentiating cells from MSCs after 14-21 days were positively stained for Ⅷ factor (vWF) related antigen by immunohistochemistry assay, and Weible-palade corpuscle was also observed by transmission electron microscopy in the cytoplasm. CONCLUSION: MSCs from HBM have the capability of differentiation into ECs in vitro, which may be a potential source of seed cells for fabrication of tissue-engineering heart valve, particularly in children with congenital heart disease.     

Bovine tuberculosis in Northern Ireland: a case-control study of herd risk factors

This paper presents the results of the first formal epidemiological study in Northern Ireland of risk factors for bovine tuberculosis (TB) associated with farm boundaries, neighbours and wildlife. The study was designed as a case-control study and 427 dairy herds were investigated between December 1993 and January 1995. The case herds had more than 30 cattle, and herds in which the source of infection had been ascribed to purchased cattle were avoided. Data on the Department of Agriculture Animal Health Computer were used in conjunction with data collected through a questionnaire to examine a range of possible risk factors, including the number and nature of the farm boundaries, the number of neighbours and their TB history, the number of hedgerows, the presence of badger setts, whether badger carcases had been found on the land, and the possible presence of deer. A follow-up telephone survey was conducted to minimise bias. The results highlighted two main associations with TB breakdowns, the presence of badgers, and contiguous neighbours who had had confirmed TB breakdowns. The estimated aetiological fraction for both associations was approximately 40 per cent, suggesting that although infected cattle may have a significant role in the transmission of TB their importance relative to the badger may have been over-estimated in the Northern Ireland TB scheme. The contribution of the badger is possibly one of several main reasons for the lack of significant progress in TB eradication despite strenuous efforts associated with all aspects of the scheme.     

Common mechanism of chromosome inversion in B- and T-cell tumors: relevance to lymphoid development

An inversion of chromosome 14 present in the tumor cells of a patient with childhood acute lymphoblastic leukemia of B-cell lineage was shown to be the result of a site-specific recombination event between an immunoglobulin heavy-chain variable gene and the joining segment of a T-cell receptor alpha chain. This rearrangement resulted in the formation of a hybrid gene, part immunoglobulin and part T-cell receptor. Furthermore, this hybrid gene was transcribed into messenger RNA with a completely open reading frame. Thus, two loci felt to be normally activated at distinct and disparate points in lymphocyte development were unified and expressed in this tumor.     

转基因棉上非靶标害虫棉蚜取食行为的EPG研究

应用EPG技术记录了棉蚜在单价转基因棉保铃棉32B、双价转基因棉国抗22以及常规棉泗棉3号上连续生活10代前后的取食行为。结果表明,棉蚜在3个棉花品种上刺吸的各种特征波型基本相同,但各波型所占比例、重复出现的频次及平均周期表现不尽一致。棉蚜在单价转Bt棉上取食时,反映被动取食行为也即主要取食行为的E波所占比例和平均周期均显著高于在常规棉上,表示未取食状态的np波所占比例和平均周期均显著低于在常规棉上;双价转基因棉上与常规棉上无差异;而反映蚜虫刺探食物行为的C波在转基因棉和常规棉间均无差异。