Metabolic mechanisms involved in the resistance of field populations of Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) to spinosad

Tuta absoluta (Meyrick) resistance to insecticides has become a significant problem in many tomato production areas in South America. New insecticides are now available for the management of this pest (i.e. spinosad), however there is scarce information about their efficacy on field populations.With the aim of determining the susceptibility of T. absoluta to spinosad we evaluated the response of second instar larvae, from five field populations (Azapa 1, Azapa 2, Lluta, Colín and Valdivia) and a laboratory reference strain (S), to a diagnostic concentration of the insecticide. We also determined the activity of the detoxifying enzymes mixed-function oxidases (MFO), glutathione-S-transferases (GST) and esterases (EST) in the same larval stage. Larval mortality in field populations was significantly lower in Azapa 1 (50.0%), Azapa 2 (44.9%), Lluta (39.9%) and Colín (53.5%) when compared to the laboratory strain (91.7%). MFO activities in field populations were between 1.8 and 4.6 times higher than those observed in the S strain, while for EST, the ratio varied from 1.7 to 14.7. The lowest ratios were observed for the GST (0.5-2.7), however, significant differences were detected for the three enzyme systems. We conclude that the evaluated mechanisms would be involved in spinosad resistance of populations of T. absoluta, presenting an increased MFO activity in all populations.     

Effect of local diets on nutritional and sensory quality of meat of indigenous goats in Tunisian arid regions

The valorization of natural resources in small ruminants feeding can reduce the cost of feed and produce good meat quality. The objective was to evaluate the effects of local feed resources on the physico‐chemical aspects, the sensorial characteristics and the fatty acid profile of goat kid's meat. Twenty‐six kids are divided in three groups (average body weight = 15.85 kg; age = 4 months). The groups received oat hay (group control C), dried olive leaves + dried Stipa tenacissima (group OL) or grass hay (group Ko). The animals were slaughtered after 90 days of experience, with an approximate final live weight of 18.5 kg. Total solids, pH, fat, crude protein, vitamin, cholesterol and fatty acid contents of meat were determined. The OL group had the highest ultimate pH (6.82 vs. 6.73); cooking loss, gross composition (total solids, protein and fat), cholesterol and colour coordinates (L, a* and b*) were similar among groups. The vitamin E, affected by diet, was higher in group OL than the other groups (3.71 mg/kg vs. 1.32 and 2.17 mg/kg, respectively, for C and Ko groups). Moreover, meat from this group showed the highest saturated fatty acid. Unsaturated fatty acids content was higher in the meat of C and Ko groups. On the other side, polyunsaturated fatty acid level was not affected by the diet treatment. The n6/n3 ratio was significantly affected by the diet; it was lower in meat of groups Ko and OL (3.17 and 3.38 respectively). The feeding effect on sensory quality of meat was not significant.     

Prevalence, antibiotic resistance, virulence traits and genetic lineages of Staphylococcus aureus in healthy sheep in Tunisia

Nasal swabs of 163 healthy sheep were obtained from two farms and one abattoir in Tunisia during 2010. Samples were inoculated in Baird Parker agar and ORSAB medium for Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) recovery, respectively. MRSA was detected in 5 of these 163 samples (3%) in ORSAB medium, and one isolate per sample was further studied. MRSA isolates were mecA-positive, typed as ST153-CC80-t044-agrIII, and contained blaZ, ant(6)-Ia, aph(3')-IIIa, erm(C), tet(K), and fusB genes encoding penicillin, streptomycin, kanamycin, erythromycin, tetracycline and fusidic acid resistance, respectively. These MRSA isolates showed indistinguishable or closely related PFGE-patterns and harboured the lukF/lukS gene encoding the Panton-Valentine leukocidin and the luk-ED, hla, hld, and hlg(v) genes. Methicillin-susceptible S. aureus (MSSA) were recovered in 68 of the 163 samples (41.7%) and one isolate per sample was characterized. Most of MSSA (82.4%) showed susceptibility to the tested antibiotics with exceptions: penicillin (6%, carrying blaZ gene), tetracycline (19%, carrying tet(K) gene) and fusidic acid (9%). The following toxin-genes were identified among MSSA: tst (53 isolates), luk-M (52), luk-ED, hla, hlb, hld and hlg(v) (67), hlg (1), sec (49), sel (52), and the egc-cluster-like sen-sem-sei-seo-seg (1). Ten spa-types (two of them new ones) and nine sequence types (six new ones) were detected among the 73 S. aureus isolates, and they were ascribed to agr types I and III. All MRSA and MSSA isolates were able to coagulate bovine plasma and MRSA harboured the immune-evasion-gene-cluster type E. Conclusions. Nares of healthy sheep could be a reservoir of PVL-positive community-associated-MRSA and also of TSST-positive S. aureus isolates, with potential implications in public health.     

Evaluation of quantified contrast-enhanced color and power Doppler ultrasonography for the assessment of vascularity and perfusion of naturally occurring tumors in dogs

OBJECTIVE: To investigate subjective and computerized methods of evaluation of color Doppler (CD) and power Doppler (PD) ultrasonographic images (obtained before and after administration of contrast medium) for quantitative assessment of vascularity and perfusion of various naturally occurring tumors in dogs. SAMPLE POPULATION: 34 tumors in 34 dogs. PROCEDURE: Tumors in dogs were examined via CD and PD ultrasonography before and after i.v. injection of a microbubble contrast agent (pre- and postcontrast examinations, respectively). Images were digitized for subjective assessment of vessel density and vascular pattern and computer-aided assessment of parameters of vascularity (fractional area [FA]) and perfusion (color-weighted FA [CWFA] and mean color-weighted FA [CWFA] and mean color level). RESULTS: With both analysis methods, more vessels were identified in precontrast PD ultrasonographic images than in precontrast CD ultrasonographic images. Moreover, compared with values for precontrast PD ultrasonography, FA, CWFA, and mean color level were higher for postcontrast PD ultrasonography. In postcontrast images, there was a significant association between vessel densities determined through subjective and computerized assessments. Although sample size was small, vascularity of squamous cell carcinomas was significantly greater than that of other tumor types. Ten of the 19 softer than issue that sarcomas had low vessel density with minor contrast enhancement. With increasing gross tumor volume, FA and CWFA decreased for all Doppler ultrasonographic methods. CONCLUSIONS AND CLINICAL RELEVANCE: Higher values of the ultrasonographic parameters representing vascularity and perfusion of tumors in dogs were determined via PD ultrasonography after administration of contrast medium than via PD or CD ultrasonography without administration of contrast medium.     

Impacts of conservation buffers and grasslands on total phosphorus loads using hydrological modeling and remote sensing techniques

To better assess the impacts of conservation buffers and grasslands on water quality at large spatial scales, development and integration of novel approaches are crucial to ensure that these land management practices are functioning properly and meeting their original goals. Recent developments in remote sensing technology have greatly enriched the availability of geospatial data that can be used in hydrological modeling to assess the potential hydrological response of conservation practices over larger areas. A methodology was developed using the object-based image analysis approach with Landsat-5 TM imagery of the year 2005 and thematic layers of streams to quantify conservation buffers and grasslands (OBIA-2005). The OBIA-2005 land cover data was used in the Soil and Water Assessment Tool hydrologic model to assess the impacts of vegetative conservation practices on total phosphorus (TP) loads. The model was calibrated and validated for discharge and TP loads in the Cedar Creek Watershed (CCW) in northeast Indiana. In general model efficiency for streamflow values was within acceptable statistical ranges. While calibration of TP loads was satisfactory for the total contributing area of two nested catchments within the upper CCW. Vegetative buffers of 30.5 m and 61 m combined with conservation grasslands generated from the OBIA-2005 resulted in a large reduction of TP loads as compared to no practices. The results also showed that including conservation grassland alone reduced TP loads by less than 2%. However, the combination of these practices with the width of edge-of-field buffer strips module of the SWAT model achieved the largest TP loads reduction. These findings demonstrate that improved representation of vegetative conservation practices in geospatial land cover data sets are more effective in assessing the impacts of conservation buffers and grasslands on water quality through hydrologic modeling.     

Calorie restriction promotes mammalian cell survival by inducing the SIRT1 deacetylase

A major cause of aging is thought to result from the cumulative effects of cell loss over time. In yeast, caloric restriction (CR) delays aging by activating the Sir2 deacetylase. Here we show that expression of mammalian Sir2 (SIRT1) is induced in CR rats as well as in human cells that are treated with serum from these animals. Insulin and insulin-like growth factor 1 (IGF-1) attenuated this response. SIRT1 deacetylates the DNA repair factor Ku70, causing it to sequester the proapoptotic factor Bax away from mitochondria, thereby inhibiting stress-induced apoptotic cell death. Thus, CR could extend life-span by inducing SIRT1 expression and promoting the long-term survival of irreplaceable cells.     

Femtotesla magnetic field measurement with magnetoresistive sensors

The measurement of magnetic fields in the femtotesla (fT, 10(-15) tesla) range is important for applications such as magnetometry, quantum computing, solid-state nuclear magnetic resonance, and magnetoencephalography. The only sensors capable of detecting these very small fields have been based on low-temperature superconducting quantum interference devices operating at 4.2 kelvin. We present a magnetic field sensor that combines a superconducting flux-to-field transformer with a low-noise giant magnetoresistive sensor. The sensor can be operated up to 77 kelvin. Our small-size prototype provides the capability of measuring 32 fT.     

Protective socking material for cultivated mussels: a potential non-disruptive deterrent to reduce losses to diving ducks

Predation of cultivated mussels by diving ducks can threaten the viability of mussel farms. Conventional scaring tactics have had limited success at deterring ducks from feeding on cultivated mussels, because of rapid habituation and 24-h feeding cycles of some waterfowl species. We tested a socking material containing a biodegradable protective layer against predation by diving ducks on experimental blue mussel (Mytilus edulis) aquaculture sites in Prince Edward Island, Canada. We also assessed the effect of this new socking material on mussel growth and survival. Results showed that the protective socking has the potential to reduce losses to ducks, but did not perform well for all mussel seed sizes used by the industry. The protective layer also did not biodegrade fast enough, trapping a portion of mussels inside the sock, affecting growth and survival of these mussels. Improvements are needed to make this solution effective for all mussel sizes used by the industry and to make its production more cost effective.