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91.
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OBJECTIVE: To evaluate the use of subdermal plexus skin flaps for closing defects after excision of cutaneous and subcutaneous tumors in dogs and to compare outcome of flaps secured with sutures and those secured with butyl-cyanoacrylate and intermittent sutures. STUDY DESIGN: Clinical study. ANIMALS: Fifteen dogs. METHODS: After excision of cutaneous or subcutaneous tumors the skin defect was reconstructed by random flaps based on the subdermal plexus. Flap skin edges were apposed with simple interrupted 4-0 monofilament nylon sutures (group 1; 5 dogs) or nylon sutures alternated with butyl-cyanoacrylate adhesive (group 2; 10 dogs). Flaps were evaluated every 48 hours when bandages were changed, until complete healing. RESULTS: Random flaps based on the subdermal plexus were effectively used to close wound defects; mean flap survival was 89%. Partial flap necrosis occurred in 4 dogs. Wound margins apposed with butyl-cyanoacrylate had thinner and more esthetic scars than sutured margins. CONCLUSION: Random flaps based on the subdermal plexus proved to be versatile for covering limb wounds after excision of cutaneous or subcutaneous tumors. Mean survival rate was comparable to that reported for axial pattern flaps. Butyl-cyanoacrylate adhesive was easy to apply, allowed accurate margin apposition with good cosmetic outcome and reduced sutures needed. CLINICAL RELEVANCE: Cyanoacrylate adhesive should be considered in lieu of suture closure to secure random skin flaps based on the subdermal plexus in dogs. 相似文献
93.
van Klaveren NJ Suwankong N De Boer S van den Brom WE Voorhout G Hazewinkel HA Meij BP 《Veterinary surgery : VS》2005,34(5):450-456
OBJECTIVE: Using force plate analysis (FPA), determine ground reaction forces in dogs with degenerative lumbosacral stenosis (DLS) and evaluate the effects of lumbosacral decompressive surgery. STUDY DESIGN: Prospective clinical study. ANIMALS: Twelve dogs with DLS. METHODS: DLS was diagnosed by clinical signs, radiography, computed tomography, and/or magnetic resonance imaging. FPA was performed before surgery, and 3 days, 6 weeks, and 6 months after surgery. The mean peak braking (Fy+), peak propulsive (Fy-), and peak vertical (Fz+) forces of 8 consecutive strides were determined. The ratio between the total Fy- of the pelvic limbs and the total Fy- of the thoracic limbs (P/TFy-), reflecting the distribution of Fy-, was analyzed to evaluate any changes in locomotion pattern postoperatively. Ground reaction force data for DLS dogs were compared with data derived from 24 healthy dogs (control). RESULTS: In dogs with DLS, the propulsive forces (Fy-) of the pelvic limbs were significantly smaller than those of controls. P/TFy- was significantly smaller in dogs with DLS than in control dogs, and increased during the follow-up period, reaching normal values 6 months after surgery. CONCLUSIONS: Cauda equina compression in dogs with DLS decreases the propulsive force of the pelvic limbs and surgical treatment restores the propulsive force of the pelvic limbs in a 6-month period. CLINICAL RELEVANCE: In dogs with DLS, FPA is an effective method in evaluating the response to surgical treatment. Normal propulsive force in the pelvic limbs was restored during 6 months after decompressive surgery. 相似文献
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秸秆覆盖条件下滨海盐渍土水盐分布及蒸发特征 总被引:1,自引:0,他引:1
为了揭示秸秆覆盖滨海盐渍土水盐调控机理,通过室内模拟实验研究了不同秸秆覆盖量(0、0.3、0.6、0.9和1.2 kg/m2分别由CK、秸秆A、秸秆B、秸秆C和秸秆D表示)对土壤水盐运移和蒸发强度的影响.结果表明,秸秆覆盖可有效提高表层及土壤剖面含水率,且增墒效果随秸秆覆盖量的增加而增加:试验期间秸秆A、秸秆B、秸秆C... 相似文献
96.
秸秆生物炭输入对冻融期黑土表层无机氮磷垂直迁移的影响 总被引:1,自引:0,他引:1
为研究秸秆生物炭输入对冻融期黑土表层无机氮磷垂直迁移的影响,采用室内模拟冻融循环试验,设置冻融与不冻融、冻融循环次数和生物炭施加量3个影响因素,分析冻融作用下不同秸秆生物炭输入量土壤表层无机氮磷垂直迁移特征。结果表明:(1)冻融与不冻融、冻融循环次数及生物炭施加量对黑土表层NO3-—N、NH4+—N和PO43-—P垂直迁移液总体积、迁移液总浓度及迁移总量均有极显著影响。(2)淋溶时间随冻融作用增强而缩短,随生物炭施加量增加而延长。所有处理迁移液总体积均随生物炭输入量的增加呈降低趋势。未冻融组迁移液总体积随培养期增加呈现缓慢下降趋势,冻融组处理迁移液总体积在第5次冻融循环试验出现急剧增加,而后趋于平稳。(3)分析冻融作用下秸秆生物炭施入对无机氮磷垂直迁移累积影响,同一控制时间内生物炭对冻融组无机氮磷垂直迁移量的抑制作用大于不冻融组,且随生物炭施入量增加对无机氮磷垂直迁移的抑制作用增强。由于土壤物理性质的改变,冻融组NO3-—N、NH4+—N和PO43-—P累积迁移量均在第5次冻融循环左右发生急剧变化。综上可知,生物炭在冻融期可以有效的固持养分,研究结果可为寒冷地区解冻期面源污染防治提供一定的理论支持。 相似文献
97.
B. M. A. De Coninck O. Amand S. L. Delauré S. Lucas N. Hias G. Weyens J. Mathys E. De Bruyne B. P. A. Cammue 《Plant pathology》2012,61(1):76-84
Cercospora leaf spot, caused by the fungus Cercospora beticola, is a major fungal sugar beet disease worldwide and the cause of significant yield losses. The disease is most successfully countered by the introduction of genetic tolerance into elite sugar beet hybrids. To this end, breeding programmes require high quality biological assays allowing discrimination of minor differences between plants within a segregating population. This study describes the successful implementation of image analysis software in the bioassays for quantification of necrotic lesions at different stages of C. beticola infection, allowing selection on minor phenotypic differences during the sugar beet breeding process for C. beticola resistance. In addition, a real‐time PCR assay was developed for the quantification of C. beticola pathogen biomass in infected beet canopy. The use of both techniques, even in an early stage of infection, fine‐tunes current bioassays, allowing more accurate and efficient selection of resistant breeding material. 相似文献
98.
Ioannis Stergiopoulos Lute-Harm Zwiers Maarten A. De Waard 《European journal of plant pathology / European Foundation for Plant Pathology》2002,108(7):719-734
This review provides an overview of members of the ATP-binding cassette (ABC) and major facilitator superfamily (MFS) of transporters identified in filamentous fungi. The most common function of these membrane proteins is to provide protection against natural toxic compounds present in the environment of fungi, such as antibiotics produced by other microorganisms. In plant pathogenic fungi, these transporters can also be an important determinant of virulence on host plants by providing protection against plant defence compounds or mediating the secretion of host-specific toxins. Furthermore, they play a critical role in determining base-line sensitivity to fungicides and other antimycotic agents. Overexpression of some of these transporters can lead to the development of resistance to chemically-unrelated compounds, a phenomenon described as multidrug resistance (MDR). This has been observed in a variety of organisms and can impose a serious threat to the effective control of pathogenic fungi. 相似文献
99.
Carolina B. Pereira Todd J. Ward Emerson M. Del Ponte Gláucia Mara Moreira Mark Busman Susan P. McCormick Heraldo R. Feksa Juliano L. De Almeida Dauri J. Tessmann 《Plant pathology》2021,70(2):426-435
We conducted a five-year survey (2011–2015) of barley and wheat fields in Paraná state, Brazil, obtaining 754 Fusarium isolates from spikes with fusarium head blight (FHB)-symptoms. Multilocus genotyping and TEF-1α gene sequence analyses confirmed the dominance of the F. graminearum species complex (FGSC, 75.7%), but F. poae (11.5%), as well as F. avenaceum and related members of the F. tricinctum species complex (FTSC, 8.1%) appeared as substantial contributors to FHB. Within the FGSC, F. graminearum of the 15-ADON genotype was dominant (63%), followed by F. meridionale of the NIV genotype (23.1%), F. cortaderiae of the NIV (7%) or 3-ADON (2.6%) genotypes, and F. austroamericanum (3.8%) of the 3-ADON genotype. Substantial variation in pathogen composition was observed across years, with F. poae and F. meridionale frequencies significantly elevated in some years. Most F. poae strains produced DAS, diANIV, and butenolide, but not neosolaniol, T-2, or HT-2. All FTSC species produced moniliformin. Enniatin production was widespread among FTSC species, with the single F. acuminatum strain found to be the strongest producer of enniatins. Our findings confirm FGSC as a major contributor to FHB and expand considerably our knowledge of the presence, frequency, and conditions under which other pathogens may emerge, altering the spectrum of toxins that may accumulate in grain. 相似文献
100.