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91.
92.
Steven De Decker DVM PhD Emma Davies DVM Diplomate ECVN Livia Benigni DVM Diplomate ECVDI Helen Wilson BVSc Ludovic Pelligand DVM PhD Diplomate ECVAA Emma L. Rayner BSc BVetMed FRCPath MRCVS Nadia Shihab MA VetMB Diplomate ECVN Holger A. Volk DVM PhD Diplomate ECVN 《Veterinary surgery : VS》2012,41(6):766-771
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Kirchner MJ Liebana E McLaren I Clifton-Hadley FA Wales AD Davies RH 《Veterinary microbiology》2012,159(3-4):509-514
To examine possible correlations in bovine Salmonella isolates between environmental survival and serovar-associated epidemiological patterns, bovine field isolates of Salmonella serovars Typhimurium and Dublin (two each) were inoculated into bovine faeces slurry and tested monthly by culture for survival during a six-month period of storage at a variable ambient temperature in a disused animal transporter. Low moisture conditions, where the slurry was dried onto wooden dowels, increased detectable survival of a low-level inoculum by up to five months, compared with wet slurry. A more modest increase of survival time was seen with storage of wet slurry under refrigeration at 4°C. Under both dry and wet conditions, the concentration of culturable Salmonella Typhimurium declined at a slower rate than did that of Salmonella Dublin. Salmonella that was naturally contaminating bovine faeces from farms with Salmonella Typhimurium did not show superior survival times compared with Salmonella Typhimurium that had been artificially inoculated into samples. The differing survival characteristics of the two serovars that was observed in environmental faeces may complement their different modes of infection in cattle. Salmonella Dublin, being a bovine host-adapted strain that establishes chronic infection in some animals, may have less need to survive for a prolonged period outside of its host than does Salmonella Typhimurium. 相似文献
94.
Intensive longitudinal investigations of breeding and growing pig populations in two multiple-site swine production systems were conducted in NC, USA. Five cohorts of sows and individually identified growing pigs from their litters were serially sampled in order to determine the prevalence and serotypes of Salmonella enterica in each stage of production based on fecal culture. In addition to fecal samples, feed and environmental samples were obtained. Fifteen different serotypes were isolated from the two systems, the most frequently isolated serotypes were S. typhimurium var Mbandaka and S. typhimurium var Copenhagen. Pig prevalence estimates ranged from 0 to 48.1%. Environmental contamination was frequently encountered despite cleaning and disinfection. Feed was rarely (2/800, 0.25%) identified as S. enterica positive. We observed highly variable patterns of S. enterica prevalence and serotype profiles within cohorts over time and among cohorts within systems. These observations indicate that point estimates of S. enterica prevalence and serotypes cannot be considered as reliable indicators of the S. enterica status of farms, and that uncontrolled studies of interventions to control S. enterica may yield misleading results. These findings are critical to the design of epidemiological studies of S. enterica on swine farms and may suggest that cohort level, as opposed to farm or company level events or management practices, may be important as potential risk factors for S. enterica fecal shedding in market age pigs. 相似文献
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AIM: To estimate over a 3-year period following the first release of rabbit haemorrhagic disease virus (RHDV) the prevalence of rabbit haemorrhagic disease (RHD) and the abundance of rabbits (Oryctolagus cuniculus) in an area that historically had low rabbit densities. METHODS: Three farms grazing predominantly sheep and beef cattle, located close together and with low initial rabbit densities, were selected for study. RHDV had been deliberately released on all farms in December 1997. Farms were visited 2–3 times per year between June 1998 and April 2001. At each visit, rabbits were shot with the aid of spotlights at night and blood samples were collected for detection of RHDV antibodies. Rabbit carcasses were necropsied and the age of the animals was determined. Rabbit abundance on each property was measured throughout the study using spotlight night counts. Logistic regression was used to identify factors associated with the risk of carcasses being seropositive for RHDV. RESULTS: Rabbit density differed initially between farms (8.2, 9.9, 2.3 rabbits per spotlight km in June 1998), and declined on all three properties over time (1.2, 2.4, 1.1 rabbits per spotlight km in November 2000). Highest antibody titres to RHDV were initially evident on the farm on which rabbits were most abundant. The average prevalence of seropositive rabbits overall was 21% (95% CI=15–28%). Female rabbits tended to be less likely to be seropositive for RHDV than males (OR=0.47; 95% CI=0.21–1.02). The odds of becoming seropositive were reduced for rabbits born in the breeding season of 1999–2000 (OR=0.17; 95% CI=0.05–0.64). CONCLUSIONS: The temporal pattern of outbreaks measured by peaks of seroprevalence differed between closely-spaced farms when they had different rabbit densities, but were similar when rabbit densities were similar. Microclimate and vegetation influencing abundance of insect vectors for RHDV and intrinsic population-related factors like rabbit breeding behaviour are also likely to be involved in local patterns of spread. 相似文献
97.
T. Quinlan B.V.Sc. D.V.C.S. B.E. Goulden B.V.Sc. Ph.D. M.R.C.V.S. M.A.C.V.Sc. A.S. Davies B.V.Sc. Ph.D. 《New Zealand veterinary journal》2013,61(7):145-147
One problem in the diagnosis of subclinical roarers is that abnormal auditory signals occur only during a fast gait, and these fade quickly. This paper offers a novel technique to assist this problem. Sound recordings were obtained from five horses following exercise; two roarers, two non-roarers, and one suspected roarer. These signals were converted into spectrograms by Rayspan processing. All frequencies in the 0 to 2.25 kHz range during both inspiration and expiration, as well as occasional noises in the 2–4 kHz band, characterised roarers. Signals from non-roarers consisted of frequencies in the 0 to 2 kHz range with long quiet periods, especially during expiration. An explanation for these phenomena is offered, and supported by diagrammatic cross-sections of equine upper-respiratory tracts. It is suggested that the present technique could assist in the diagnosis of sub- clinical roarers, estimated to be between 20 and 40% of the competitive horse population. 相似文献
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