Biomonitoring of atmospheric mercury in the vicinity of Kilauea,Hawaii

The release of mercury from volcanic eruptions on the Island of Hawaii has been well documented, but the dispersion characteristics have not previously been studied in detail. Samples of lichens were collected from around the island, and subsequent analysis showed levels ranging from < 8 to 59 µg Hg g^-1. The distribution of the accumulation of mercury clearly shows that there is a major input from the active eruption sites, but that this is significantly modified by variations in wind patterns. Superimposed on this regional distribution are two localised sites where the mercury accumulation is exceptionally high; this is attributed to the release of steam from the circulation of shallow ground waters.     

世界猪病的发展与演变(续)

7 病原-宿主关系的改变以及宿主内的疾病过程 如上所述,改变宿主和疾病病原之间的关系可能会使病原自身发生变化,或者会引起宿主之间的疾病过程变化以及传染原在宿主间的传播变化。 通常来说,当宿主很少暴露给特定病原时,由于缺少先天性抵抗力(由遗传决定)和未经免疫的宿主,从而使最严重的疾病症状得以发生,易感年龄间变化最小。宿主先天性抵抗力可能受到一定程度的自然因素或人为的管理因素作用,尤其是在最严重或最麻烦的疾病方     

Micropropagation of Cryptanthus with leaf explants with attached intercalary meristems excised from greenhouse stock plants

By using the leaves with attached intercalary meristems from greenhouse grown stock plants, five cultivars of Cryptanthus were cultured on modified MS media with 4.5 μM NAA and IBA and 3 μM BA to induce adventitious shoot formation from callus tissue. Contamination was 17–21% for explants taken from stock plants which were sprayed weekly with Agribrom and 27–75% for those taken from stock plants which were not treated. More than 99% true to type plantlets were obtained from non-chimeric plants. Green and albino plantlets were obtained from chimeric plants. The chimeric C. ‘Coster's Favorite’ DeCoster also produced a few chimeric plantlets with intermarginal pink stripes in addition to the green and albino plantlets. Most of the non-chimeric plants took a shorter time to produce plantlets of transplantable size (8–12 mm) than the chimeric ones. Except for albino plantlets, survival rate of plantlets exceeded 95%. A minimum average of 500 rooted plantlets can be obtained in a year from a single well-callused leaf explant. The protocol in this report should speed up the mass production and introduction of desirable new cultivars and hybrids of non-chimeric Cryptanthus.     

Outer membrane protein profiles of Yersinia ruckeri

The outer membrane protein (OMP) profiles of 135 isolates of Yersinia ruckeri, obtained from nine European countries (100 isolates), North America (23 isolates), Australia (six isolates) and South Africa (two isolates), and including four reference strains, were examined by SDS-PAGE. Outer membranes were isolated by selective solubilisation of the cytoplasmic membrane with 0.5% (w/v) sodium N-lauroyl sarcosinate (Sarkosyl). Outer membrane proteins were stable after in vitro passage and there was no variation in OMP profiles due to colony selection. With the exception of a 39.5 kDa peptidoglycan-associated protein there was also no variation at different stages of the growth cycle. The 39.5 kDa protein was not produced during logarithmic growth phase but increased in abundance as the stationary phase progressed. Interstrain variation occurred in the possession of a 36.5 or 38 kDa heat-modifiable protein and in the possession of peptidoglycan-associated proteins in the molecular weight range 36.5 to 40.5 kDa. Based on variation of these proteins five OMP-types, designated OMP-types 1-5, were identified among the 135 isolates examined. Outer membrane protein analysis was demonstrated to be useful in epidemiological studies of Y. ruckeri.     

Strain variation in Dermatophilus congolensis demonstrated by cross-protection studies

Cross-protection studies were conducted with vaccines prepared from two isolates of Dermatophilus congolensis (designated strain 1 and strain 2). The vaccines were prepared as either heat-inactivated, washed, formalized filamentous phase bacterium, mixed with alum as an adjuvant, and inoculated intramuscularly (type A vaccine) or sedimented live filaments inoculated intradermally (type B vaccine). The vaccinated sheep were challenged with D. congolensis zoospores of one or other strain. Challenge sites were observed for the presence and severity of lesions. Serum antibody levels to D. congolensis were monitored after vaccination and challenge. Type A and B vaccines from both strains produced some reduction in the severity of lesions when sheep were challenged with strain 1 but not with strain 2. Unvaccinated control sheep developed more severe and persistent lesions when challenged with strain 2 than controls challenged with strain 1. Serum antibody levels to the type B vaccine prepared from strain 1 were significantly higher (P less than 0.05) than antibody levels to type B vaccine from strain 2. These findings showed there was significant variation in virulence and antigenicity between these two isolates of D. congolensis.     

Evaluation of methods used for detecting Streptococcus suis type 2 in tonsils, and investigation of the carrier state in pigs

Streptococcus suis type 2 was isolated from the tonsils of 143 (76 per cent) of 188 pigs from three herds by culturing on media containing a specific antiserum. Culture of frozen sections of tonsillar tissue was more sensitive than culture of swabs or deep scrapes. The specificity of an indirect fluorescent antibody test for identifying S suis type 2 in mixed cultures was conservatively estimated to be 0.89. The level of infection in one herd without a history of meningitis was lower than in two herds which had experienced repeated outbreaks. Differences between herds in the level of infection were due to differences in the number of organisms carried by pigs, more so than differences in the detectable carrier rate.     

Musculoskeletal Growth Gradients: A Contribution to Quadrupedal Mechanics

Postnatally, the axial skeleton of the pig shows peaks of high relative growth at the sites of attachment of the fore and hindlimbs, corresponding to the regions of highest relative growth in the limbs. This supports the concept that musculoskeletal growth gradients are a propulsive adaptation.     

Use of limestone wastes from metal mining as agricultural lime: potential heavy metal limitations

Abstract. Finely ground limestone waste from lead mining is discarded as useless but could be a valuable alternative to agricultural limestone. A glasshouse pot experiment established that it is an effective liming material. Two kinds were used: one from north Wales (HMT) represented metal-rich waste produced by the older water-gravity separation of ores; the other (NLB) was a modern flotation tailings. A commercial lime (CLS) was used for comparison. The materials were applied to an acid soil in quantities sufficient to raise the pH to 7 (single liming) and at much greater rates equivalent to annual limings for 369 years. All treatments were replicated five times and the soil/lime mixtures were analysed for EDTA-extractable lead, zinc and cadmium. Radish was grown as the test plant and hypocotyl and leaf tissue were analysed for the same metals. The larger additions of HMT and NLB caused chlorosis and decreased yields. The cadmium and lead concentrations of leaves and hypocotyls suggested that the HMT material could be safely applied to soil annually for 79 years and NLB for 277 years. It is concluded that NLB can be used as agricultural limestone.