Obituary: Dr Sam Jamieson,OBE, MRSVS,DVSM, PhD

Extract

Dr Sam Jamieson died suddenly at his home on 11 May 1987, aged 77 years     

Surveillance of Parapoxvirus Among Ruminants in Virginia and Connecticut

In 2008, two deer hunters in Virginia and Connecticut were infected with a unique strain of pseudocowpox virus, a parapoxvirus. To estimate the prevalence of this virus, and in an attempt to define the reservoir, Parapoxvirus surveillance was undertaken between November 2009 and January 2010. 125 samples from four ruminant species (cows, goat, sheep and white‐tailed deer) were collected in Virginia, and nine samples from white‐tailed deer were collected in Connecticut. We found no evidence that the parapoxvirus species that infected the deer hunters is circulating among domesticated ruminants or white‐tailed deer. However, parapoxvirus DNA of a different parapoxvirus species, bovine papular stomatitis virus (BPSV), was detected in 31 samples obtained from asymptomatic cattle in Virginia. Parapoxvirus DNA–positive cattle originated from the same counties indicating probable transmission among animals. Molecular analysis identified BPSV as the parapoxvirus affecting animals. Asymptomatic parapoxvirus infections in livestock, particularly young animals, may be common, and further investigation will inform our knowledge of virus transmission.     

A preliminary investigation of Ehrlichia species in ticks, humans, dogs, and capybaras from Brazil

A molecular epidemiologic investigation in two Brazilian states (Rondônia and São Paulo) was undertaken to determine if Ehrlichia species responsible for human and animal ehrlichioses in North America could be found in Brazilian vectors, potential natural mammalian reservoirs and febrile human patients with a tick bite history. Samples, including 376 ticks comprising 9 Amblyomma species, 29 capybara (Hydrochaeris hydrochaeris) spleens, 5 canine blood, and 75 human blood samples from febrile patients with history of tick bites were tested by a real-time PCR assay targeting a fragment of the Ehrlichia dsb gene. Ehrlichia DNA was not detected in any tick, capybara or human samples. In contrast, 4 out of 5 dogs contained Ehrlichia canis DNA in their blood, which were sequenced, representing the first report of E. canis infecting dogs in the Amazon region of Brazil. Further studies are needed to evaluate the presence of other agents of human and animal ehrlichioses in Brazil.     

Impact of farm management on expression of early mortality syndrome/acute hepatopancreatic necrosis disease (EMS/AHPND) on penaeid shrimp farms in Thailand

Asian shrimp farming industry has experienced massive production losses due to a disease caused by toxins of Vibrio bacteria, known as early mortality syndrome/acute hepatopancreatic necrosis disease (EMS/AHPND) for the last 5 years. The disease can cause up to 100% cumulative pond mortality within a week. The objective of this study was to identify factors associated with AHPND occurrence on shrimp farms. A case–control study was carried out on shrimp farms in four provinces of Thailand. Factors related to farm characteristics, farm management, pond and water preparation, feed management, post‐larvae (PL) shrimp and stock management were evaluated. Multivariable logistic regression analysis identified factors affecting AHPND occurrence at the pond level. Chlorine treatment, reservoir availability, use of predator fish in the water preparation, culture of multiple shrimp species in one farm and increased PL stocking density contributed to an increased risk of AHPND infection, while delayed first day of feeding, polyculture and water ageing were likely to promote outbreak protection. Additionally, the source of PL was found to be associated with AHPND occurrence in shrimp ponds, which requires further study at the hatchery level. Identification of these factors will facilitate the development of effective control strategies for AHPND on shrimp farms.     

Determining the disaggregated economic value of irrigation water in the Musi sub-basin in India

In this paper the residual method is used to determine the disaggregated economic value of irrigation water used in agriculture across crops, zones and seasons. This method relies on the belief that the value of a good (its price by its quantity) is equal to the summation of the quantity of each input multiplied by its average value. By applying this method to the Musi sub-basin; a subdivision of the Krishna basin in India, it was found that the value of irrigation water use is not equated across the crops, zones and seasons. The reasons why there is no sinlge value of irrigation water use are suggested. As farmers do not have perfect knowledge, do not all possess the same resource base, plant different crops for a variety of reasons (some for a financial return on land instead of water and others for sustenance), possess different crop rotation practices and are possibly riks adverse, they all value water differently. However, the results need to be interpreted with care as the crop with the lowest return to water is probably not the one to be sacrificed if water is restricted, since farmers plant crops for a variety of reasons (and sometimes not for the highest return to water that they can achieve).     

Fillet Quality and Processing Attributes of Postsmolt Atlantic Salmon,Salmo salar,Fed a Fishmeal‐free Diet and a Fishmeal‐based Diet in Recirculation Aquaculture Systems

Many studies have evaluated the adequacy of alternate ingredient diets for Atlantic salmon, Salmo salar, mainly with focus on fish performance and health; however, comprehensive analysis of fillet quality is lacking, particularly for salmon fed these diets in recirculation aquaculture systems (RAS). To this end, a study was conducted comparing fillet quality and processing attributes of postsmolt Atlantic salmon fed a fishmeal‐free diet (FMF) versus a standard fishmeal‐based diet, in replicate RAS. Mean weight of Atlantic salmon fed both diets was 1.72 kg following the 6‐mo trial and survival was >99%. Diet did not affect (P > 0.05) processing and fillet yields, whole‐body proximate composition(fat, moisture, protein), fillet proximate composition, cook yield, fillet texture, color, or omega‐3 fatty acid fillet content, including eicosapentaenoic acid and docosahexaenoic acid levels. Whole‐body ash content was greater in salmon fed the FMF diet. The FMF diet resulted in a wild fish‐in to farmed fish‐out ratio of 0:1 per Monterey Bay Aquarium's Seafood Watch criteria due to its fishmeal‐free status and use of lipids from fishery byproduct. Overall, fillet quality and processing attributes were generally unaffected when feeding a diet devoid of fishmeal to postsmolt Atlantic salmon cultured in RAS. [Correction added on 7 September 2017, after first online publication: the P value in Abstract has been changed from “P < 0.05” to “P > 0.05”.].     

Field and laboratory transmission studies of haemic neoplasia in the soft‐shell clam,Mya arenaria,from Atlantic Canada

A two‐year laboratory and field study was initiated in 2001 in response to mass mortalities associated with haemic neoplasia (HN) in 1999 in Prince Edward Island (PEI) soft‐shell clams, Mya arenaria. A laboratory proximity experiment (cohabitation) and an inoculation challenge were conducted with clams and mussels (Mytilus edulis). Three field exposure experiments were also conducted, in which naive clams were held in sediment (in trays) or out of sediment (in mesh bags) at three high HN prevalence sites on PEI. There was a conversion to HN positive in clams in the proximity experiment and in clams injected with whole blood and cell‐free homogenate, but not at statistically significant levels. No mussels or control clams became HN positive. There was a significant conversion to HN positive in as little as 24 and 58 days after transfer with clams held out of sediment and in sediment, respectively. The laboratory and field experiments’ results suggest that HN‐infected clams are spreading the disease through water from infected clams to naïve individuals and via transplantation from affected to unaffected sites. Some environmental conditions (e.g. abnormally high water temperature and hypoxia‐induced sea lettuce [Ulva lacteus] invasion) may make clams susceptible to infections or exacerbate the proliferation of HN.     

Three scales of temporal resolution from automated soil respiration measurements

Soil respiration (R_s) is a combination of autotrophic and heterotrophic respiration, but it is often modeled as a single efflux process, influenced by environmental variables similarly across all time scales. Continued progress in understanding sources of variation in soil CO₂ efflux will require development of R_s models that incorporate environmental influences at multiple time scales. Coherence analysis, which requires high temporal frequency data on R_s and related environmental variables, permits examination of covariation between R_s and the factors that influence it at varying temporal frequencies, thus isolating the factors important at each time scale. Automated R_s measurements, along with air, soil temperature and moisture were collected at half hour intervals at a temperate forest at Harvard Forest, MA in 2003 and a boreal transition forest at the Howland Forest, ME in 2005. As in other temperate and boreal forests, seasonal variation in R_s was strongly correlated with soil temperature. The organic and mineral layer water contents were significantly related to R_s at synoptic time scales of 2–3 days to weeks, representing the wetting and drying of the soils as weather patterns move across the region. Post-wetting pulses of R_s were correlated with the amount of precipitation and the magnitude of the change from pre-wet-up moisture content to peak moisture content of the organic horizon during the precipitation events. Although soil temperature at 8–10 cm depth and R_s showed strong coherence at a 24-h interval, calculated diel Q₁₀ values for R_s were unreasonably high (6–74) during all months for the evergreen forest and during the growing season for the deciduous forest, suggesting that other factors that covary with soil temperature, such as canopy assimilatory processes, may also influence the diel amplitude of R_s. Lower diel Q₁₀ values were obtained based on soil temperature measured at shallower depths or with air temperature, but the fit was poorer and a lag was needed to improve the fit (peak R_s followed peak air temperature by several hours), suggesting a role for delayed substrate supply from aboveground processes to affect diel patterns of R_s. High frequency automated R_s datasets afford the opportunity to disentangle the temporal scales at which environmental factors, such as seasonal temperature and phenology, synoptic weather events and soil moisture, and diel variation in temperature and photosynthesis, affect soil respiration processes.