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991.
Patterson PH 《Science (New York, N.Y.)》2007,318(5850):576-577
992.
To identify cytoskeletal proteins that change conformation or assembly within stressed cells, in situ labeling of sterically shielded cysteines with fluorophores was analyzed by fluorescence imaging, quantitative mass spectrometry, and sequential two-dye labeling. Within red blood cells, shotgun labeling showed that shielded cysteines in the two isoforms of the cytoskeletal protein spectrin were increasingly labeled as a function of shear stress and time, indicative of forced unfolding of specific domains. Within mesenchymal stem cells-as a prototypical adherent cell-nonmuscle myosin IIA and vimentin are just two of the cytoskeletal proteins identified that show differential labeling in tensed versus drug-relaxed cells. Cysteine labeling of proteins within live cells can thus be used to fluorescently map out sites of molecular-scale deformation, and the results also suggest means to colocalize signaling events such as phosphorylation with forced unfolding. 相似文献
993.
Vermetten E Bremner JD Skelton L Spiegel D 《Science (New York, N.Y.)》2007,315(5809):184-7; author reply 184-7
994.
Current biogeographic models hypothesize that brown bears migrated from Asia to the New World ~100 to 50 thousand years ago but did not reach areas south of Beringia until ~13 to 12 thousand years ago, after the opening of a mid-continental ice-free corridor. We report a 26-thousand-year-old brown bear fossil from central Alberta, well south of Beringia. Mitochondrial DNA recovered from the specimen shows that it belongs to the same clade of bears inhabiting southern Canada and the northern United States today and that modern brown bears in this region are probably descended from populations that persisted south of the southern glacial margin during the Last Glacial Maximum. 相似文献
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998.
A century ago, W. G. MacCallum identified distinct male and female forms in malaria parasites of both birds and humans. Since then, scientists have been puzzled by the high female-to-male ratios of parasites in Plasmodium infections and by the mechanism of sex determination. The sex ratio of malaria parasites was shown to become progressively more male as conditions that allow motility and subsequent fertilization by the male parasites become adverse. This resulted from an increased immune response against male gametes, which coincides with intense host erythropoietic activity. Natural and artificial induction of erythropoiesis in vertebrate hosts provoked a shift toward male parasite production. This change in parasite sex ratio led to reduced reproductive success in the parasite, which suggests that sex determination is adaptive and is regulated by the hematologic state of the host. 相似文献
999.
David H. Potten 《Computers and Electronics in Agriculture》1985,1(1):111-118
Since 1980 there has been huge growth in the commercial use of computers in relation to daily farming activities in the U.K. Three main types of application are involved, Bureau services, stand-alone computers on the farm, and on-line services such as Prestel Farmlink through the farmer's television set.Over 120 companies offer computer services to farmers, and 88 were surveyed for this study. Between them they offered 253 different packages of which the most common are for dairy and pig herd management, financial management, arable crops management and other livestock applications.There are no hard data on the number of farmers who use computers, but the proportion is greater than in most other countries. At present at least a third of the companies in the sector have formed joint ventures to market existing packages or develop new applications, and the recent rapid pace of change in the sector is expected to continue. 相似文献
1000.
The calling song of male field crickets is composed of stereotyped rhythmic pulse intervals, which are predictable expressions of genotype. Females identify conspecific males by their song. Two species of crickets were found to exhibit species-specific song preference, and hybrids between them preferred hybrid calls over either parental call. These results imply genetic control of song reception as well as transmission. 相似文献