Background, Aims and Scope Our study determined the role of light, aeration, grain size, amount of sediments and of water, density of animals and presence
of additional food on the movement of Ilyanassa obsoleta. Spiked relative to reference sediments were also tested for avoidance or preference by the mud snails. Our goal was to develop
exposure conditions to be used in future assessment studies of the environmental quality of sediments inhabited by mud snails.
The behavioural response of the snails towards different concentrations of contaminated Halifax Harbour sediments placed in
one half of a tank relative to reference sediments in the other half is examined in Part 2 and a chemical link investigated.
Methods Animals were exposed in 7 L aquaria at 12°C to their native sediments placed in one or two halves of a tank and the effect
due to the presence of a light source, air bubbles, sediment grain size, added algae examined relative to the snails’ behaviour.
Preference or avoidance of one half of a tank relative to the second half, the immersed or dry glass surface represented the
various media to be chosen by snails. After choosing the optimum set up, exposures were performed in triplicate, under slightly
differing initial placement of the snails to compare means and variability in the results. Three specific biogenic chemicals,
cholesterol, coprostanol and a fatty acid methyl ester (FAME) were spiked at 0.002, 0.01, 0.05, 0.25 and 1.2 mg/g, dry weight
of sediment. A hexane, dichloromethane and methanol extract of harbour sediment were also spiked in reference sediments. In
all cases, the location of the snails was recorded over a period extending up to 96 hrs.
Results Twenty snails exposed during a 72 hrs period in commercial 7 L tanks containing 50 g of sediments (wet weight) in the bottom
half of a tank filled half way with seawater represented the chosen conditions to test the avoidance/preference behaviour
of snails for part of a tank. The presence of additional food on sediments attracted snails within 24 hrs of exposure.
Discussion A general lack of preference was indicated in exposures to three biologically derived compounds spiked in reference sediments,
i.e. a C-18 FAME, cholesterol and coprostanol. The repelling properties of a harbour sediment extract pointed to a role for
non polar chemicals.
Conclusions It is proposed that behaviour reflects a balance between the repelling effects of contaminants and the attractive value of
food. The present study provides a robust green assessment tool that is easy to set up to assess the quality of sediments.
Recommendations and Perspectives The interpretation of field observations regarding the abundance of animals during environmental assessments must consider
both nutritional and toxicological causes. Additional laboratory exposures are needed to determine which chemicals play a
role in the field. 相似文献
Dryland ecosystems have long been considered to have a highly heterogeneous distribution of nutrients and soil biota, with greater concentrations of both in soils under plants relative to interspace soils. We examined the distribution of soil resources in two plant communities (dominated by either the shrub Coleogyne ramosissima or the grass Stipa hymenoides) at two locations. Interspace soils were covered either by early successional biological soil crusts (BSCs) or by later successional BSCs (dominated by nitrogen (N)-fixing cyanobacteria and lichens). For each of the 8 plant type×crust type×locations, we sampled the stem, dripline, and 3 interspace distances around each of 3 plants. Soil analyses revealed that only available potassium (Kav) and ammonium concentrations were consistently greater under plants (7 of 8 sites and 6 of 8 sites, respectively). Nitrate and iron (Fe) were greater under plants at 4 sites, while all other nutrients were greater under plants at less than 50% of the sites. In contrast, calcium, copper, clay, phosphorus (P), and zinc were often greater in the interspace than under the plants. Soil microbial biomass was always greater under the plant compared to the interspace. The community composition of N-fixing bacteria was highly variable, with no distinguishable patterns among microsites. Bacterivorous nematodes and rotifers were consistently more abundant under plants (8 and 7 sites, respectively), and fungivorous and omnivorous nematodes were greater under plants at 5 of the 8 sites. Abundance of other soil biota was greater under plants at less than 50% of the sites, but highly correlated with the availability of N, P, Kav, and Fe. Unlike other ecosystems, the soil biota was only infrequently correlated with organic matter. Lack of plant-driven heterogeneity in soils of this ecosystem is likely due to (1) interspace soils covered with BSCs, (2) little incorporation of above-ground plant litter into soils, and/or (3) root deployment patterns. 相似文献
AIMS: To assess the attitude of dairy farm consultants to the role of veterinarians on dairy farms and whether they viewed veterinary advice as impartial, free from commercial bias and central to farm productivity and profitability.
METHODS: A telephone survey was commissioned in September 2014 of 36 full-time farm consultants in the Canterbury and Otago region of New Zealand using a pre-prepared questionnaire to explore their attitudes towards the veterinary profession. Of those approached, 25 completed the survey.
RESULTS: The consultants surveyed agreed or strongly agreed (21/23) that they were the custodians of animal health expenditure on farms, which was seen as an expense to be controlled (19/25 agreed or strongly agreed). This view was held more strongly (9/9 agreed or strongly agreed) by consultants with >5 years’ professional experience, compared to 10/16 consultants with ≤5 years’ experience. Most consultants (24/25) disagreed that they did not respect veterinary advice, but agreed (24/25) that veterinarians almost always had a vested interest in the advice that they gave. The role of veterinarians was seen by respondents to be mainly treatment of sick animals (22/25) and provision of animal health products (24/25), but not in maximising farm profitability (selected by 8/25 respondents). Consultants viewed their own roles as providing advice on the influence of animal health on farm profitability (21/25), nutrition (22/25) and reproduction (20/25). Most respondents (21/25) stated that reducing disease and animal health issues was important, but they (21/25) also identified a reduction in farm animal health expenditure as a measure of success. Only 7/25 consultants felt that they should work in partnership with veterinarians.
CONCLUSIONS: This was a small scale study from one region of New Zealand but it indicates that consultants are ambivalent about the role and value of working more closely with veterinarians, and about the benefit that veterinary intervention may bring to a farm’s profitability.
CLINICAL RELEVANCE: More successful veterinary involvement on dairy farms will follow from a better understanding of how other rural professionals are involved in the farm’s management and in meeting the individual farmer’s goals and motivations. 相似文献
Previously, three distinct populations of putative primordial germ cells (PGCs), namely gonocytes, intermediate cells and pre‐spermatogonia, have been described in the human foetal testis. According to our knowledge, these PGCs have not been studied in any other species. The aim of our study was to identify similar PGC populations in canine embryos. First, we develop a protocol for canine embryo isolation. Following our protocol, 15 canine embryos at 21–25 days of pregnancy were isolated by ovaryhysterectomy surgery. Our data indicate that dramatic changes occur in canine embryo development and PGCs specification between 21 to 25 days of gestation. At that moment, only two PGC populations with distinct morphology can be identified by histological analyses. Cell population 1 presented round nuclei with prominent nucleolus and a high nuclear to cytoplasm ratio, showing gonocyte morphology. Cell population 2 was often localized at the periphery of the testicular cords and presented typical features of PGC. Both germ cell populations were positively immunostained with anti‐human OCT‐4 antibody. However, at day 25, all cells of population 1 reacted positively with OCT‐4, whereas in population 2, fewer cells were positive for this marker. These two PGCs populations present morphological features similar to gonocytes and intermediate cells from human foetal testis. It is expected that a population of pre‐spermatogonia would be observed at later stages of canine foetus development. We also showed that anti‐human OCT‐4 antibody can be useful to identify canine PGC in vivo.相似文献
An analysis of historical sea surface temperatures provides evidence for global warming since 1900, in line with land-based analyses of global temperature trends, and also shows that over the same period, the eastern equatorial Pacific cooled and the zonal sea surface temperature gradient strengthened. Recent theoretical studies have predicted such a pattern as a response of the coupled ocean-atmosphere system to an exogenous heating of the tropical atmosphere. This pattern, however, is not reproduced by the complex ocean-atmosphere circulation models currently used to simulate the climatic response to increased greenhouse gases. Its presence is likely to lessen the mean 20th-century global temperature change in model simulations. 相似文献