全文获取类型
收费全文 | 9867篇 |
免费 | 3657篇 |
国内免费 | 2篇 |
专业分类
林业 | 479篇 |
农学 | 497篇 |
基础科学 | 17篇 |
1915篇 | |
综合类 | 643篇 |
农作物 | 246篇 |
水产渔业 | 2794篇 |
畜牧兽医 | 5330篇 |
园艺 | 121篇 |
植物保护 | 1484篇 |
出版年
2023年 | 40篇 |
2022年 | 67篇 |
2021年 | 217篇 |
2020年 | 578篇 |
2019年 | 1141篇 |
2018年 | 1019篇 |
2017年 | 1035篇 |
2016年 | 1045篇 |
2015年 | 890篇 |
2014年 | 920篇 |
2013年 | 1142篇 |
2012年 | 694篇 |
2011年 | 766篇 |
2010年 | 747篇 |
2009年 | 389篇 |
2008年 | 471篇 |
2007年 | 321篇 |
2006年 | 308篇 |
2005年 | 282篇 |
2004年 | 270篇 |
2003年 | 276篇 |
2002年 | 241篇 |
2001年 | 97篇 |
2000年 | 118篇 |
1999年 | 42篇 |
1998年 | 27篇 |
1997年 | 28篇 |
1996年 | 20篇 |
1995年 | 18篇 |
1994年 | 23篇 |
1993年 | 17篇 |
1992年 | 18篇 |
1991年 | 20篇 |
1990年 | 21篇 |
1989年 | 14篇 |
1988年 | 15篇 |
1986年 | 14篇 |
1985年 | 10篇 |
1984年 | 16篇 |
1983年 | 8篇 |
1982年 | 11篇 |
1981年 | 8篇 |
1980年 | 7篇 |
1978年 | 12篇 |
1977年 | 9篇 |
1974年 | 8篇 |
1971年 | 6篇 |
1970年 | 7篇 |
1925年 | 6篇 |
1924年 | 6篇 |
排序方式: 共有10000条查询结果,搜索用时 843 毫秒
991.
Lugo J Harkema JR deFeijter-Rupp H Bartner L Boruta D Robinson NE 《Veterinary journal (London, England : 1997)》2006,172(2):293-301
This study was performed to determine if a peripheral sample of lung from the site where biopsy is conducted is representative of the rest of the lung and to investigate the relationship between airway inflammation and intraepithelial mucous production in the peripheral airways. Lung parenchyma samples were collected from five different regions of the lung in five control and five heaves-affected horses. Horse groups were defined by clinical response to stabling. Tissue sections were used for semi-quantitative scoring of lesions, to count the number of airways, to quantify the amount of stored mucosubstances (Vs) within the epithelium, and to count the number of epithelial cells in terminal airways. No significant differences were found between lung regions or between groups of horses. Lack of regional differences in airway structures means that a biopsy sample can be used for diagnosis and investigation of diffusely distributed diseases. Airway inflammation was correlated with mucous cell metaplasia and Vs. Therefore, in horses, mucus accumulation is partly caused by increased number of mucous cells and is associated with airway inflammation. Therapy targeted to reduce airway inflammation will help reduce the excessive mucous accumulation in horses. 相似文献
992.
Picque D Lieben P Corrieu G Cantagrel R Lablanquie O Snakkers G 《Journal of agricultural and food chemistry》2006,54(15):5220-5226
Mid-infrared spectroscopy was applied to the analysis and discrimination of Cognacs and other distilled drinks (Armagnacs, whiskies, brandies, bourbons, rums, and counterfeit products). Strong correlations were found between dry extract spectra, polyphenolic dry extract spectra, and the total polyphenol concentration of samples, notably of Cognacs. Principal component analysis applied to spectral data made it possible to emphasize the importance of dry extract data when a distinction is made between Cognacs and Armagnacs, whiskies, bourbons, and rums, and of polyphenol concentration when Cognacs, brandies, and counterfeit products are separated. Ninety-six percent of samples in the test set were correctly assigned to Cognacs and non-Cognacs by partial least-squares discriminant analysis. 相似文献
993.
OBJECTIVE: The purpose of the present study was to characterise the food landscape of an inner city African American neighbourhood and its mixed-race suburban neighbour. Detailed analysis focuses on the relationship between community store mix and price, availability and produce quality. DESIGN: A market basket study was completed by members of the Chicago Food Systems Collaborative. The US Department of Agriculture's standard market basket survey and methodology were used. Additional items and analyses were added in consultation with community members. SETTING: Austin is a lower-middle-class African American community of 117,500 on the western edge of Chicago. Oak Park, which borders Austin, is an upper-middle-income suburb of 52,500 with a mixed racial profile.Subjects: A market basket survey of every retail food store in Austin and Oak Park was completed. A total of 134 were included. RESULTS: Results indicate that Austin has many grocery stores and few supermarkets. Many Austin groceries stores carry produce that is usually competitively priced, but often of unacceptable quality. Supermarkets had the best selection. Prices were lowest at discount supermarkets. Prices of packaged items were higher at independent stores than at chain supermarkets, but fresh items were cheaper. CONCLUSIONS: Food access is related more to store type than number. In this study, item availability and produce quality varied greatly between store types. Price differences were complicated and varied by store type and food category. This has consequences in terms of food purchasing decisions and dietary quality that public health professionals should acknowledge. 相似文献
994.
Sandford SA Aléon J Alexander CM Araki T Bajt S Baratta GA Borg J Bradley JP Brownlee DE Brucato JR Burchell MJ Busemann H Butterworth A Clemett SJ Cody G Colangeli L Cooper G D'Hendecourt L Djouadi Z Dworkin JP Ferrini G Fleckenstein H Flynn GJ Franchi IA Fries M Gilles MK Glavin DP Gounelle M Grossemy F Jacobsen C Keller LP Kilcoyne AL Leitner J Matrajt G Meibom A Mennella V Mostefaoui S Nittler LR Palumbo ME Papanastassiou DA Robert F Rotundi A Snead CJ Spencer MK Stadermann FJ Steele A 《Science (New York, N.Y.)》2006,314(5806):1720-1724
Organics found in comet 81P/Wild 2 samples show a heterogeneous and unequilibrated distribution in abundance and composition. Some organics are similar, but not identical, to those in interplanetary dust particles and carbonaceous meteorites. A class of aromatic-poor organic material is also present. The organics are rich in oxygen and nitrogen compared with meteoritic organics. Aromatic compounds are present, but the samples tend to be relatively poorer in aromatics than are meteorites and interplanetary dust particles. The presence of deuterium and nitrogen-15 excesses suggest that some organics have an interstellar/protostellar heritage. Although the variable extent of modification of these materials by impact capture is not yet fully constrained, a diverse suite of organic compounds is present and identifiable within the returned samples. 相似文献
995.
Thrice out of Africa: ancient and recent expansions of the honey bee, Apis mellifera 总被引:1,自引:0,他引:1
Whitfield CW Behura SK Berlocher SH Clark AG Johnston JS Sheppard WS Smith DR Suarez AV Weaver D Tsutsui ND 《Science (New York, N.Y.)》2006,314(5799):642-645
We characterized Apis mellifera in both native and introduced ranges using 1136 single-nucleotide polymorphisms genotyped in 341 individuals. Our results indicate that A. mellifera originated in Africa and expanded into Eurasia at least twice, resulting in populations in eastern and western Europe that are geographically close but genetically distant. A third expansion in the New World has involved the near-replacement of previously introduced "European" honey bees by descendants of more recently introduced A. m. scutellata ("African" or "killer" bees). Our analyses of spatial transects and temporal series in the New World revealed differential replacement of alleles derived from eastern versus western Europe, with admixture evident in all individuals. 相似文献
996.
Foster MS Edwards MS Reed DC Schiel DR Zimmerman RC 《Science (New York, N.Y.)》2006,313(5794):1737-9; author reply 1737-9
997.
Chen ZY Jing D Bath KG Ieraci A Khan T Siao CJ Herrera DG Toth M Yang C McEwen BS Hempstead BL Lee FS 《Science (New York, N.Y.)》2006,314(5796):140-143
A common single-nucleotide polymorphism in the brain-derived neurotrophic factor (BDNF) gene, a methionine (Met) substitution for valine (Val) at codon 66 (Val66Met), is associated with alterations in brain anatomy and memory, but its relevance to clinical disorders is unclear. We generated a variant BDNF mouse (BDNF(Met/Met)) that reproduces the phenotypic hallmarks in humans with the variant allele. BDNF(Met) was expressed in brain at normal levels, but its secretion from neurons was defective. When placed in stressful settings, BDNF(Met/Met) mice exhibited increased anxiety-related behaviors that were not normalized by the antidepressant, fluoxetine. A variant BDNF may thus play a key role in genetic predispositions to anxiety and depressive disorders. 相似文献
998.
Eshaghi S Niegowski D Kohl A Martinez Molina D Lesley SA Nordlund P 《Science (New York, N.Y.)》2006,313(5785):354-357
CorA family members are ubiquitously distributed transporters of divalent metal cations and are considered to be the primary Mg2+ transporter of Bacteria and Archaea. We have determined a 2.9 angstrom resolution structure of CorA from Thermotoga maritima that reveals a pentameric cone-shaped protein. Two potential regulatory metal binding sites are found in the N-terminal domain that bind both Mg2+ and Co2+. The structure of CorA supports an efflux system involving dehydration and rehydration of divalent metal ions potentially mediated by a ring of conserved aspartate residues at the cytoplasmic entrance and a carbonyl funnel at the periplasmic side of the pore. 相似文献
999.
Coastal wetlands of the northern Gulf of California: inventory and conservation status 总被引:1,自引:0,他引:1
Edward P. Glenn Pamela L. Nagler Richard C. Brusca Osvel Hinojosa‐Huerta 《水产资源保护:海洋与淡水生态系统》2006,16(1):5-28
- 1. Above 28°N, the coastline of the northern Gulf of California is indented at frequent intervals by negative or inverse estuaries that are saltier at their backs than at their mouths due to the lack of freshwater inflow. These ‘esteros’ total over 215 000 ha in area and encompass mangrove marshes below 29°N and saltgrass (Distichlis palmeri) marshes north of 29°N. An additional 6000 ha of freshwater and brackish wetlands are found in the Colorado River delta where fresh water enters the intertidal zone.
- 2. The mangrove marshes in the Gulf of California have been afforded some degree of protected status in Mexico, but the northern saltgrass esteros do not have priority conservation status and are increasingly becoming development targets for resorts, vacation homes and aquaculture sites.
- 3. We conducted an inventory of the marshes using aerial photography and satellite images, and evaluated the extent and type of development on each marsh. We reviewed the available literature on the marshes to document their vegetation types and ecological functions in the adjacent marine and terrestrial ecosystems.
- 4. Over 95% of the mangrove marshes have been developed for shrimp farming. However, the farms are built adjacent to, rather than in, the marshes, and the mangrove stands are still mostly intact.
- 5. The majority of saltgrass marshes above the mangrove line are still relatively unspoiled. However, resort and vacation home development is taking place on land surrounding them.
- 6. We recommend a system of protected reserves incorporating the pristine wetlands, along with water quality management and buffer zones for the more developed esteros. The saltgrass marshes should be considered for conservation protection, similar to the protection given to the southern mangrove marshes whose value has already been recognized.
1000.
Polikepahad S Paulsen DB Moore RM Costa LR Venugopal CS 《American journal of veterinary research》2006,67(2):348-357
OBJECTIVE: To immunohistochemically determine the expression of endothelin (ET) receptors in bronchial smooth muscle and epithelium of healthy horses and horses affected by summer pasture-associated obstructive pulmonary disease (SPAOPD). SAMPLE POPULATION: Tissue specimens obtained from 8 healthy and 8 SPAOPD-affected horses. PROCEDURE: Horses were examined and assigned to healthy and SPAOPD groups. Horses were then euthanatized, and tissue specimens containing bronchi of approximately 4 to 8 mm in diameter were immediately collected from all lung lobes, fixed in zinc-formalin solution for 12 hours, and embedded in paraffin. Polyclonal primary antibodies against ET-A or ET-B receptors at a dilution of 1:200 and biotinylated IgG secondary antibodies were applied to tissue sections, followed by the addition of an avidin-biotin immunoperoxidase complex. Photographs of the stained slides were digitally recorded and analyzed by use of image analysis software to determine the intensity of staining. Two-way ANOVA was used for statistical analysis. RESULTS: The left diaphragmatic lung lobe of SPAOPD-affected horses had a significantly greater area of bronchial smooth muscle that immunostained for ET-A, compared with that for healthy horses. All lung lobes of SPAOPD-affected horses, except for the right diaphragmatic lobe, had significantly greater staining for ET-B receptors in bronchial smooth muscle, compared with results for healthy horses. CONCLUSIONS AND CLINICAL RELEVANCE: This study revealed overexpression of ET-A and, in particular, ETB receptors in the bronchial smooth muscle of SPAOPD-affected horses, which suggested upregulation of these receptors. These findings improve our understanding of the role of ET-1 in the pathogenesis of SPAOPD. 相似文献