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91.
The application of tannic acid to the elimination of egg stickiness at varied moments of the egg swelling process in pikeperch,Sander lucioperca (L.)
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Daniel Żarski Sławomir Krejszeff Dariusz Kucharczyk Katarzyna Palińska‐Żarska Katarzyna Targońska Krzysztof Kupren Pascal Fontaine Patrick Kestemont 《Aquaculture Research》2015,46(2):324-334
The aim of this article was to analyse the process of pikeperch, Sander lucioperca, egg swelling and to apply tannic acid to eliminate egg stickiness at different moments of the swelling process on artificially obtained eggs. The first experiment involved observation of egg swelling process and the second determined the effect of temperature (12, 14 and 16°C) on the egg swelling rate. The third experiment involved elimination of egg stickiness in a tannin solution (0.75 g L?1) where eggs were submerged in a solution for 0.5, 1, 2 and 5 min – 5, 10, 15, 20, 25 and 30 min following gamete activation. The results indicate that the pikeperch egg swelling process lasts 30 min. It was found that the temperature did not affect the process duration. The results of the third experiment showed that the effectiveness of tannic acid application in egg stickiness elimination increases with time. The best result was obtained in groups of eggs submerged for 1 and 2 min (86.5% and 80.5% of larvae were obtained respectively) 30 min following the gamete activation. The results presented in this study for the first time indicate the possibility of highly effective procedure of egg stickiness elimination with tannic acid in pikeperch aquaculture. 相似文献
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A qPCR assay was developed for rapid and sensitive detection of Flavobacterium psychrophilum, the aetiological agent of bacterial cold-water disease and rainbow trout fry syndrome in salmonid fish worldwide. A set of F. psychrophilum-specific primers based on 16S rRNA gene sequences was designed and validated for specific detection and quantification of DNA isolated from representative strains of F. psychrophilum. The qPCR assay exhibited a high specificity for the 16S rRNA gene of F. psychrophilum (from 4 × 10(8) down to 11 copies per reaction) but not for other Flavobacterium species or other bacteria including fish pathogens. This qPCR-based method proved to be useful in the quantification of the F. psychrophilum titre present within organs dissected out from diseased fish. As the F. psychrophilum genome contains six copies of the 16S rRNA gene, we could infer a limit of detection corresponding to two bacteria per reaction, corresponding to 800 bacteria per fish tissue sample, and therefore 20 F. psychrophilum cells mg(-1) of tissue (for sample weighing 40 mg). The qPCR assay reported here could be a useful tool for veterinary diagnostic laboratories to monitor the F. psychrophilum infection level in fish farms. 相似文献
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The effects of hydraulic dredging on the benthic infauna of a mud bottom area were investigated in Georgia in the Atlantic Intracoastal Waterway. Complete displacement of the benthic community was caused by dredging; however, the community began to recover within a month following the cessation of dredging. Within two months the diversity and species composition of the dredged channel were similar to that of a control area and little change in sediment composition resulted. Thus, no apparent limitation was imposed on the normal benthic population by habitat alteration. Recolonisation was too rapid to have been caused by the settling of immature or larval stages from the water column alone. Bank slumping and migration of adult forms have been postulated as other means of recolonisation. 相似文献
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Karina Janice Guadalupe Díaz-Resendiz Carlos Eduardo Covantes-Rosales Alma Betsaida Benítez-Trinidad Migdalia Sarahy Navidad-Murrieta Francisco Fabian Razura-Carmona Christian Daniel Carrillo-Cruz Edwin Jaime Frias-Delgadillo Daniela Alejandra Prez-Díaz Matxil Violeta Díaz-Benavides Mercedes Zambrano-Soria Guadalupe Herminia Ventura-Ramn Aurelio Romero-Castro David Alam-Escamilla Manuel Ivn Girn-Prez 《Marine drugs》2022,20(2)
Fucoidan is a polysaccharide obtained from marine brown algae, with anti-inflammatory, anti-viral, and immune-enhancing properties, thus, fucoidan may be used as an alternative treatment (complementary to prescribed medical therapy) for COVID-19 recovery. This work aimed to determine the ex-vivo effects of treatment with fucoidan (20 µg/mL) on mitochondrial membrane potential (ΔΨm, using a cationic cyanine dye, 3,3′-dihexyloxacarbocyanine iodide (DiOC6(3)) on human peripheral blood mononuclear cells (HPBMC) isolated from healthy control (HC) subjects, COVID-19 patients (C-19), and subjects that recently recovered from COVID-19 (R1, 40 ± 13 days after infection). In addition, ex-vivo treatment with fucoidan (20 and 50 µg/mL) was evaluated on ΔΨm loss induced by carbonyl cyanide 3-chlorophenylhydrazone (CCCP, 150 µM) in HPBMC isolated from healthy subjects (H) and recovered subjects at 11 months post-COVID-19 (R2, 335 ± 20 days after infection). Data indicate that SARS-CoV-2 infection induces HPBMC loss of ΔΨm, even 11 months after infection, however, fucoidan promotes recovery of ΔΨm in PBMCs from COVID-19 recovered subjects. Therefore, fucoidan may be a potential treatment to diminish long-term sequelae from COVID-19, using mitochondria as a therapeutic target for the recovery of cellular homeostasis. 相似文献
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First account of a transient intersex in spotted scat,Scatophagus argus: a marine gonochoristic fish
Mustapha Umar Farouk Zhi Fei Huang Yuang-Qing Assan Daniel Li Guang-Li Jiang Dong-Neng 《Fish physiology and biochemistry》2022,48(4):1011-1023
Fish Physiology and Biochemistry - This study presents the first incidence of intersex associated with testis-ova in spotted scat (Scatophagus argus) reared in a controlled environment. The... 相似文献