Synthesis and Identification of Artificial Antigen for Imidacloprid

This study reported that a hapten of imidacloprid, 1-[(6-Carboxylethylthio-3- pyridinyl)methyl)-N-nitro-imidazolidinimine was synthesized using technical material of imidacloprid to react with 3-mercaptopropionic acid in hot alkaline solution. The hapten was conjugated to bovine serum albumin (BSA) and ovalbumin (OVA) to form the immuno-antigens and the coating antigen, respectively. The antibody with high titer (2.56 × 10⁴) was obtained after immuning rabbits. The results showed that the antibody had a high affinity to imidacloprid tested by enzyme-linked immunosorbent assay (ELISA), which IC₅₀ and IC₂₀ were 20.7 and 1.1 μg L⁻¹; and the cross reactibilities to those compounds related with imidacloprid were less than 1.4%.     

同羊春毛理化特性的研究

研究结果表明,同羊春毛纤维类型数量比例较30年前有所改善.比秋毛弯曲明显整齐,弯曲率大,光泽好,摩擦效应大;17种氨基酸含量及总量也多于秋毛;伸度和强度的集中性及含硫量差于秋毛;表面结构也不及秋毛好,特别是含硫量还低于羊毛一般水平.     

火地塘林区油松林的群落类型及结构特征的研究

通过Noy－Meir分离法和PCA排序分析，把火地塘林区油松天然林的优势灌木划分为照山白、绿叶胡枝子、粉背黄栌、华北绣线菊等4个生态种组；据此将油松群系划分为3个群丛组。绿叶胡枝子—油松林和黄栌—油松林这两个群丛组多为单层同世代异龄林，其演替趋向为松栎混交林；照山白十六道木—油松林群丛组为复层多世代异龄林，是相对稳定的以油松为优势的群落。     

薄皮甜瓜杂交一代新品种"甜罐"特征特性及栽培技术

甜瓜，因其生态类型不同，分薄皮甜瓜和厚皮甜瓜两大类型。我们经过几年的科研开发，培育出薄皮甜瓜杂交一代新品种——甜罐，不仅完全保留了薄皮甜瓜的特点，又增加了抗重茬性和耐贮运性能。     

Expression of interleukin-10 activity by Epstein-Barr virus protein BCRF1

Cytokine synthesis inhibitory factor (CSIF; interleukin-10), a product of mouse TH2 T cell clones that inhibits synthesis of cytokines by mouse TH1 T cell clones, exhibits extensive sequence similarity to an uncharacterized open reading frame in the Epstein-Barr virus BCRF1. Recombinant BCRF1 protein mimics the activity of interleukin-10, suggesting that BCRF1 may have a role in the interaction of the virus with the host's immune system.     

Lenalidomide alone or combined with bortezomib inhibits growth and induces apoptosis in KM3 cells

AIM: To investigate the effects of lenalidomide alone or combined with bortezomib on induction of apoptosis in multiple myeloma(MM) KM3 cells.METHODS: The KM3 cells were treated with lenalidomide alone or combined with bortezomib. The number of viable cells was determined by trypan blue exclusion. Apoptotic cells were simultaneously stained with annexin V-FITC and PI and determined by flow cytometry. RT-PCR was used to examine the level of p65 mRNA. Western blotting was used to examine the protein levels of P65, pP65, caspase-3,-8, -9, and poly (ADP-ribose) polymerase (PARP). RESULTS: Lenalidomide-inhibited the cell growth and induced apoptosis of KM3 cells. The mechanism responsible for lenalidomide-mediated inhibition was via NF-κB and activation of caspase-mediated induction of apoptosis. A synergistic effect was observed when combination of lenalidomide with bortezomib was used. CONCLUSION: Lenalidomide inhibits the growth and induces apoptosis in KM3 cells. There is a synergistical effect when combination of lenalidomide with bortezomib is in use.     

Comparison of biological parameters between the invasive B biotype and a new defined Cv biotype of <Emphasis Type="Italic">Bemisia tabaci</Emphasis> (Hemiptera: Aleyradidae) in China

The sweetpotato whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) is a widely distributed and destructive agricultural pest on various host plants. The biology of two biotypes of B. tabaci: the invasive B and a new defined Cv biotype, on a range of host plants (hibiscus, laurel, poinsettia, collard, cucumber and tomato) were studied in the laboratory. Results revealed that the developmental periods of the B biotype immatures were not significantly different on the tested host plants except those between laurel and collard. The Cv biotype immatures developed significantly slower on cucumber and tomato than on the other plants. B. tabaci B biotype had the highest survivorship on collard (68.55%), and the lowest on laurel (33.24%), while the Cv biotype had the highest and lowest survivorships on laurel (61.63%) and tomato (36.74%). Host plants did not significantly affect the pre-ovipostion period regardless of biotype. The longest averaged longevity and highest fecundity of B biotype were both recorded on collard: 25.15 days and 143.0 eggs. The highest fecundity of Cv biotype was 196.49 eggs on laurel and its longest longevity was on hibiscus (19.62 days). The intrinsic rate of natural increase (r _m) of B biotype on the three vegetables were all higher than those on the three ornamentals whereas the r _m of Cv biotype on the three ornamentals were all higher that those on the three vegetables. Our research indicates that B. tabaci B and Cv biotypes have different host plant suitabilities. The three tested vegetables were more suitable for B biotype while the three tested ornamental plants were more suitable for Cv biotype. The potential mechanism for the different suitability of B and Cv biotypes on various host plants is also discussed.     

三种黑土中有机碳、氮、磷的形态分布与肥力的关系

土壤腐殖质是土壤的重要组分。土壤的许多属性都直接或间接地与腐殖质的性质有关。我们曾指出^[2]黑土的腐殖物质及其组分与土壤的物理、化学及生物化学有显著的相关性,这表明它们对氮、磷等营养物质的转化、供应及贮存起着重要的作用。     

Evaluation of fly ash,apatite and rice straw derived-biochar in varying combinations for in situ remediation of soils contaminated with multiple heavy metals

ABSTRACT

In

-situ sorbent amendment is a relatively low-cost, low-impact approach for remediation of soil contaminated with heavy metals (HMs), and thus is considered a way to be favored in developing countries. In this study, materials of non-hazardous, alkaline agronomic and industrial by-products were used as sorbents to explore their capacity of in situ immobilization of multiple HMs in mining-impacted arable soil. These sorbents included fly ash (FA), biochar (BC) and apatite (AP) and they were implemented with varying ratios of combinations. Results of soil microcosm tests showed that after incubation for 90 days, concentrations of Pb, Zn, and Cd in their exchangeable forms determined by a sequential extraction method significantly decreased in amended soils, as opposed to the unamended control. Of the five sets of amendments, the composite of FA, BC, and AP resulted in the maximum reduction (up to 80%) in the mobility of Pb, Zn, and Cd in soils. The mechanisms underlying the immobilization of HMs in amended soils might involve processes of surface precipitation, ion exchange and complexation, in which the physicochemical properties of sorbent materials played an important role. The immobilization efficacy of sorbent amendments on HMs in soil was further supported by pot experiments in which significant inhibition of HM accumulation in the belowground and aboveground tissues of maize was observed after 50-day cultivation in amended soils as compared with control soil. Together, these results suggest that the application of cost-saving and environmentally friendly materials derived from wastes as sorbents to remediate soils contaminated with multiple HMs is promising for developing countries like Vietnam.     

宁南干旱山区春小麦氮、磷施肥模式研究

该用二次饱和Ｄ最优设计，研究了试区３种不同立地农田生态系统春小麦Ｎ、Ｐ施肥效应方程，建立起不同适宜性的施肥数学模型，分析研究了Ｎ、Ｐ肥的主效应和交互效应，计算出不同农田的最佳施肥量，以低位梯田为例，计算出不同产量下Ｎ、Ｐ肥的用量及最适配比。