野生细叶百合组织培养体系的建立

以细叶百合为试材,采用组织培养的方法,研究细叶百合不同部位、不同激素配比及不同发生途径对获得细叶百合再生植株的影响。结果表明:以鳞茎为外植体,通过器官发生途径获得再生植株,其最佳分化及增殖培养基为MS+6-BA 1.0mg/L+NAA 0.1mg/L;以花器官、幼嫩叶片为外植体通过体细胞发生途径获得再生植株,其愈伤组织诱导培养基为MS+2,4-D2.0mg/L+NAA 1.5mg/L,不定芽诱导培养基为MS+6-BA 1.0mg/L+IAA 0.1mg/L,最佳增殖培养基为MS+6-BA 0.5mg/L+NAA 0.1mg/L,生根培养基为1/2MS+NAA 0.1mg/L。     

高校图书馆2.0服务模式的构建

分析了图书馆2.0的背景和发展现状,从信息资源、网络技术、人力资源和服务平台等方面论述了图书馆2.0服务模式的构成四要素,从服务平台的设计、搜索引擎的功能、学科专业讨论区的设计、RSS功能和用户My library的建立等6个方面探讨了高校图书馆2.0服务模式的构建。     

荣昌猪与新荣昌猪Ⅰ系遗传变异的RAPD标记分析

利用60个随机引物对荣昌猪与新荣昌猪Ⅰ系的基因组DNA进行RAPD分析,其中35个引物可扩增出清晰的RAPD带型,1个引物A1表现出明显的遗传多态性。结果表明：荣昌猪和新荣昌猪Ⅰ系群内平均相似系数分别为0．787,0．80;平均遗传距离为0．213,0．198,两品系间平均相似系数为0．795;平均遗传距离为0．206,两品系内及品系间都存在着DNA分子水平上的差异。     

动物检疫电子出证中常见问题的处理办法

针对官方兽医在动物检疫电子出证过程中常犯的一些错误和遇到的一些疑惑,提出了处理方法,结合甘肃省武威市凉州区在动物检疫过程中取得的经验,就完善电子出证系统提出了两点建议。     

Production of Ban miniature pig embryos by in vitro fertilization: A comparative study with Landrace

Ban is an endangered miniature pig breed in Vietnam. This study aimed to set up an in vitro embryo production (IVP) system for this breed. Ban's epididymal sperm concentration (1240 ± 35 × 10⁶/mL) was lower (P < 0.01) compared with Landrace (4160 ± 42 × 10⁶/mL). However, sperm characteristics before and after freezing in Ban and Landrace were similar. The numbers of follicles with diameter larger than 2 mm per ovary in Ban females treated with equine chorionic gonadotropin and human chorionic gonadotropin (27.1 ± 1.3) were higher (P < 0.05) than those in Landrace (12.9 ± 2.0) and in non‐hormone stimulated Ban (no > 2 mm follicles). After in vitro maturation, the percentages of oocytes with expanded cumulus cells and the first polar body (matured oocytes) were not different among Ban, hormone‐stimulated Ban and Landrace. The percentages of two‐cell embryos and morulae derived from oocytes collected from three sources did not differ. However, the rate of blastocysts derived from oocytes in non‐stimulated Ban (4.0 ± 3.8%) was lower (P < 0.05) than that in Landrace (15.3 ± 1.8%). In conclusion, an effective IVP system for good quality embryos in Ban, that is essential for genetic conservation of this breed, was established.     

Parameterization and testing of a coupled photosynthesis-stomatal conductance model for boreal trees

A coupled photosynthesis-stomatal conductance model was parameterized and tested with branches of black spruce (Picea mariana (Mill.) B.S.P.) and jack pine (Pinus banksiana Lamb.) trees growing in the Northern Study Area of the Boreal Ecosystem-Atmosphere Study (BOREAS) in Manitoba, Canada. Branch samples containing foliage of all age-classes were harvested from a lowland old black spruce (OBS) and an old jack pine (OJP) stand and the responses of photosynthesis (A(n)) and stomatal conductance (g(s)) to temperature, CO(2), light, and leaf-to-air vapor pressure difference (VPD) were determined under controlled laboratory conditions at the beginning, middle, and end of the growing season (Intensive Field Campaigns (IFC) 1, 2, and 3, respectively). The parameterized model was then tested against in situ field gas-exchange measurements in a young jack pine (YJP) and an upland black spruce (UBS) stand as well as in the OBS and OJP stands. Parameterization showed that Rubisco capacity (V(max)), apparent quantum yield (alpha') and Q(10) for sink limitation were the most crucial parameters for the photosynthesis sub-model and that V(max) varied among different measurement series in the laboratory. Verification of the model against the data used to parameterize it yielded correlation coefficients (r) of 0.97 and 0.93 for black spruce and jack pine, respectively, when IFC-specific parameters were used, and 0.77 and 0.87 when IFC-2 parameters were applied to all IFCs. For both measured and modeled g(s), the stomatal conductance sub-model, which linearly relates g(s) to (A(n)h(s))/c(s) (where h(s) and c(s) are relative humidity and CO(2) mole fraction at the leaf surface, respectively), had significantly steeper slopes and higher r values when only the VPD response data were used for parameterization than when all of the response data were used for parameterization. Testing the photosynthesis sub-model against upper canopy field data yielded poor results when laboratory estimates of V(max) were used. Use of the mean V(max) estimated for all upper canopy branches measured on a given day improved model performance for jack pine (from a nonsignificant correlation between measured and modeled A(n) to r = 0.45), but not for black spruce (r = 0.45 for both cases). However, when V(max) was estimated for each branch sample individually, the model accurately predicted the 23 to 137% diurnal variation in A(n) for all stands for both the upper and lower canopy. This was true both when all of the other parameters were IFC-specific (r = 0.93 and 0.92 for black spruce and jack pine, respectively) and when only mid-growing season (IFC-2) values were used (r = 0.92 for both species). Branch-specific V(max) estimates also permitted accurate prediction of field g(s) (r = 0.75 and 0.89 for black spruce and jack pine, respectively), although parameterization with all of the response data overestimated g(s) in the field, whereas parameterization with only the VPD response data provided unbiased predictions. Thus, after parameterization with the laboratory data, accurately modeling the range of A(n) and g(s) encountered in the field for both black spruce and jack pine was reduced to a single unknown parameter, V(max).     

兔害防治措施及评价

该文对目前兔害防治使用的十几种措施进行了阐述，并对铁(钢)丝索套捕兔法、生物防治、P-1拒避剂防治、政策性收购等方法给以肯定，对树干涂白灰、林地洒羊血、灭鼠雷炸兔、电网捕捉、氧化乐果废柴油涂干等措施予以否定，针对化学杀鼠剂防治兔害出现的误杀有益动物、毒杀天敌动物的严重问题，明确指出野外大面积使用化学杀鼠剂是错误的，必须禁止或限制使用，即使对于大隆、溴敌隆第二代抗凝血药剂，也只有在防治地下害兽和进行药饵投洞防治时才可使用。提醒人们在兔害防治中应牢固地树立保护生态环境和保护天敌动物的观念。     

黄淮冬麦区超高产小麦品种的产量结构模式研究

采用相关和回归分析 ,研究了黄淮冬麦区超高产小麦品种的产量结构及其相互间的关系。结果表明 :多穗型品种要实现超高产必须三要素协调 ,在保证足够穗数的前提下 ,提高穗粒数和千粒重 ,其最佳产量结构为穗数 6 80 6× 10 6 穗 /hm2 左右 ,穗粒数 38 16粒左右 ,千粒重4 0 34g左右 ;大穗型品种实现超高产的关键是提高穗粒数和千粒重 ,其最佳产量结构为穗数4 6 76× 10 6 穗 /hm2 左右 ,穗粒数 4 9 73粒左右 ,千粒重 4 9 38g左右。     

良种茶树采摘加工信阳毛尖茶关键技术研究

信阳毛尖茶生产对茶树品种的基本要求是发芽早、芽叶绿色多茸毛,抗寒性强,在化学特性上要求茶多酚含量不超过25%,氨基酸含量高于3%,酚氨值小于7;按照“鲜叶采摘—鲜叶摊放—杀青—做形—干燥”工艺生产的信阳毛尖茶,其针型系列,主要是以采摘灌木型中、小叶良种茶树1芽1叶初展至1芽2叶的鲜叶生产,产品外形特征是色泽翠绿、外形圆细紧直、形似松针;而芽型系列,主要以小乔木型良种茶树的芽或1芽1叶初展的鲜叶生产,产品外形特征是外形似芽、形直、白毫满披。