全文获取类型
收费全文 | 135658篇 |
免费 | 7789篇 |
国内免费 | 70篇 |
专业分类
林业 | 5576篇 |
农学 | 4644篇 |
基础科学 | 863篇 |
17485篇 | |
综合类 | 18963篇 |
农作物 | 5088篇 |
水产渔业 | 7091篇 |
畜牧兽医 | 72978篇 |
园艺 | 1800篇 |
植物保护 | 9029篇 |
出版年
2020年 | 1173篇 |
2019年 | 1410篇 |
2018年 | 2230篇 |
2017年 | 2490篇 |
2016年 | 2295篇 |
2015年 | 1999篇 |
2014年 | 2451篇 |
2013年 | 5732篇 |
2012年 | 4351篇 |
2011年 | 5167篇 |
2010年 | 3503篇 |
2009年 | 3547篇 |
2008年 | 5153篇 |
2007年 | 4971篇 |
2006年 | 4643篇 |
2005年 | 4264篇 |
2004年 | 4108篇 |
2003年 | 4179篇 |
2002年 | 3808篇 |
2001年 | 4773篇 |
2000年 | 4641篇 |
1999年 | 3747篇 |
1998年 | 1472篇 |
1997年 | 1452篇 |
1996年 | 1342篇 |
1995年 | 1599篇 |
1994年 | 1327篇 |
1993年 | 1359篇 |
1992年 | 2762篇 |
1991年 | 2901篇 |
1990年 | 2866篇 |
1989年 | 2897篇 |
1988年 | 2610篇 |
1987年 | 2592篇 |
1986年 | 2539篇 |
1985年 | 2400篇 |
1984年 | 1956篇 |
1983年 | 1716篇 |
1982年 | 1154篇 |
1979年 | 1649篇 |
1978年 | 1284篇 |
1977年 | 1135篇 |
1976年 | 1100篇 |
1975年 | 1164篇 |
1974年 | 1358篇 |
1973年 | 1449篇 |
1972年 | 1378篇 |
1971年 | 1195篇 |
1970年 | 1247篇 |
1969年 | 1146篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
991.
Three abortigenic Indian isolates of equine herpesvirus-1 (EHV-1) (Tohana, Hisar and Bikaner), along with two exotic abortigenic
isolates (AB4 and V592) and another EHV-1 isolate (Jind) obtained from a case of perinatal foal mortality, were studied for
variability. For this purpose, PCR and restriction endonuclease (RE) digestion techniques were used simultaneously as a DNA
fingerprinting system. Nine different regions of EHV-1 virus were amplified by PCR using primer pairs specific for the regions
and the products obtained from these regions were subsequently subjected to various restriction endonucleases to further assess
the variability in the number of RE sites as well as in their positions. No difference was observed in all the four abortigenic
isolates in terms of the size of different PCR products amplified by all the nine primer pairs, except for primer pairs ‘E’
and ‘C’. PCR products obtained with primer pair E revealed that Tohana and Bikaner isolates were most similar while Hisar
isolate was like V592 isolate. However, the PCR product obtained from Jind isolate had a size between the PCR products of
Hisar and Tohan/Bikaner isolates. The primer pair ‘C’ used to amplify the region between 1151 to 3679 in ‘Gene 1,2,3’ clearly
differentiated the EHV-1 isolate obtained from a case of perinatal foal mortality from isolates obtained from abortion cases.
This primer pair needs to be exploited more extensively for use as a potential marker for differentiating the EHV-1 isolates,
mainly the abortion cases from perinatal foal mortality ones. Restriction endonuclease studies done with PCR product of all
the isolates with various primer pairs did not reveal any changes in the position or number of RE sites present in the products
amplified, indicating no variation in different RE sites within the amplified PCR products. However, this study clarified
that all the Indian isolates belonged to the IP group of EHV-1. 相似文献
992.
A model to evaluate economic criteria involved when cattle are raised on high-forage diets prior to finishing or finished directly after weaning was developed using data from two experiments. In Exp. 1, each year for 3 yr, 136 Charolais-cross calves were weaned and allotted to either an intensive system, in which they were immediately finished on a high-grain diet, or an extensive system, in which they were wintered on crop residues, grazed on summer pasture and finished on a high-grain diet. In Exp. 2, 160 British breed steers were wintered, in one of eight different wintering systems utilizing crop residues, using supplemental protein and(or) alfalfa hay. After wintering, the steers grazed summer pasture and then were finished on a high-grain diet. Overall cost of gain and final "break-even" price were lower for cattle finished through the extensive system except when the price of corn was very low in relationship to other inputs. Interest costs were higher for cattle in the extensive system. Increasing the feeder calf purchase price had almost no effect on differences between the systems. Corn price and purchase price affected both systems similarly, whereas interest rate, wintering yardage and finishing yardage affected each system differently. Because of the additional weight produced through the extensive system, it yielded lower final "break-even" prices in most situations. 相似文献
993.
Hemophilia A in three unrelated cats 总被引:1,自引:0,他引:1
S M Cotter R M Brenner W J Dodds 《Journal of the American Veterinary Medical Association》1978,172(2):166-168
Factor VIII deficiency, consistent with hemophilia A, was detected in 3 unrelated male domestic short-haired cats. Clinical history of each case differed and illustrated the spectrum of clinical manifestations of this disease in cats. Clinical expression of feline hemophilia A was similar to that of man and other animals, in which the degree of factor VIII deficiency tends to determine the severity and frequency of bleeding. All 3 cats had severe protracted bleeding after minor surgical procedures. Hemorrhage was eventually controlled in each case by transfusions of fresh whole blood. 相似文献
994.
Kumar V Madabushi R Lucchesi MB Derendorf H 《Journal of veterinary pharmacology and therapeutics》2011,34(2):130-135
Kumar, V., Madabushi, R., Lucchesi, M. B. B., Derendorf, H. Pharmacokinetics of cefpodoxime in plasma and subcutaneous fluid following oral administration of cefpodoxime proxetil in male beagle dogs. J. vet. Pharmacol. Therap. 34 , 130–135. Pharmacokinetics of cefpodoxime in plasma (total concentration) and subcutaneous fluid (free concentration using microdialysis) was investigated in dogs following single oral administration of prodrug cefpodoxime proxetil (equivalent to 5 and 10 mg/kg of cefpodoxime). In a cross over study design, six dogs per dose were utilized after a 1 week washout period. Plasma, microdialysate, and urine samples were collected upto 24 h and analyzed using high performance liquid chromatography. The average maximum concentration (Cmax) of cefpodoxime in plasma was 13.66 (±6.30) and 27.14 (±4.56) μg/mL with elimination half‐life (t1/2) of 3.01 (±0.49) and 4.72 (±1.46) h following 5 and 10 mg/kg dose, respectively. The respective average area under the curve (AUC0–∞) was 82.94 (±30.17) and 107.71 (±30.79) μg·h/mL. Cefpodoxime was readily distributed to skin and average free Cmax in subcutaneous fluid was 1.70 (±0.55) and 3.06 (±0.93) μg/mL at the two doses. Urinary excretion (unchanged cefpodoxime) was the major elimination route. Comparison of subcutaneous fluid concentrations using pharmacokinetic/pharmacodynamic indices of fT>MIC indicated that at 10 mg/kg dose; cefpodoxime would yield good therapeutic outcome in skin infections for bacteria with MIC50 upto 0.5 μg/mL while higher doses (or more frequent dosing) may be needed for bacteria with higher MICs. High urine concentrations suggested cefpodoxime use for urinary infections in dogs. 相似文献
995.
K. Tagata S. Yokoyama T. Ginbo M. Honda T. Okimura M. Odakura M. Nomura S. Yamamoto 《Veterinary research communications》1996,20(1):21-30
A capillary reversed passive latex agglutination test (capillary RPLA) was developed which allows quantification of serum C-reactive protein (CRP) within approximately 15 min. The logarithmic regression line (calibration curve) obtained after measuring each CRP concentration three times in twofold dilutions of a standard canine serum containing 222 g/ml of CRP was y=6.394+0.030x (r=0.995). Capillary RPLA permitted quantification of CRP in the range 6.9–222 g/ml. The coefficients of variation ranged from 10.28% to 12.40%. The recovery rates (percentage recovery) of CRP by capillary RPLA were within the range 87% to 106%. On measuring the CRP concentrations in sera from 78 dogs by capillary RPLA, single radial immunodiffusion (SRID) and enzyme-linked immunosorbent assay (ELISA), close correlations were demonstrated between SRID and capillary RPLA (y=7.250+1.109x, r=0.978), between SRID and ELISA (y=3.042+1.059x, r=0.967), and between capillary RPLA and ELISA (y=1.778+0.929x, r=0.962). Capillary RPLA may be considered useful as a routine biochemical technique for measurement of serum CRP concentration in the dog.Abbreviations CRP
C-reactive protein
- ELISA
enzyme-linked immunosorbent assay
- RPLA
reversed passive latex agglutination test
- SRID
single radial immunodiffusion 相似文献
996.
Effect of oat decortication on chemical composition,in vitro digestibility and in situ degradability
Farhad M. Panah Saman Lashkari Martin Riis Weisbjerg 《Journal of animal physiology and animal nutrition》2020,104(1):109-115
The effect of decortication on chemical composition and degradability characteristics of four Danish oat varieties was investigated. Effective degradability (ED) and post-ruminal disappearance (PRD) were measured by in situ and mobile bag techniques respectively. Decorticated oat showed higher (p = .01) concentrations of crude protein (CP; 134 vs. 108 g/kg DM) and crude fat (71.6 vs. 53.1 g/kg DM) and a higher (p = .001) organic matter digestibility (OMD; 888 vs. 703 g/kg OM) than oat. The content of total fatty acids (FA) in DM was higher in decorticated oat. The proportion of linoleic acid (C18:2 n6) increased (p < .05) due to decortication, while the linolenic acid (C18:3 n3) proportion of total FA decreased in decorticated oat. Decortication increased (p = .01) the concentration of amino acids (AA), but the proportion of lysine in total AA decreased (p < .002). Effective degradability (ED) of both DM and CP was (p < .001) higher in decorticated oat. Decortication increased the total tract disappearance (TTD) and PRD of CP (p < .001). In conclusion, decortication can be used as a practical approach to increase the nutritional value of oat. 相似文献
997.
The physiological response of Atlantic salmon, Salmo salar L., to a single experimental challenge with sea lice, Lepeophtheirus salmonis 总被引:1,自引:0,他引:1
J M Bowers A Mustafa D J Speare G A Conboy M Brimacombe D E Sims J F Burka 《Journal of fish diseases》2000,23(3):165-172
The sea louse, Lepeophtheirus salmonis , is an ectoparasitic copepod of Atlantic salmon, Salmo salar L., capable of causing severe damage. This study was conducted to examine the physiological response of salmon to the stress of sea lice infestation. Smoltified salmon were acclimatized in 30‰ saltwater and exposed to high levels of lice infestation. The number of copepods per fish ranged from 15 to 285, with a mean of 106. The infested salmon were sampled six times over the 29-d experimental duration and examined for alterations in the primary and secondary stress indicators, including plasma concentrations of cortisol, glucose, electrolytes, thyroid hormones T3 and T4, as well as the haematocrit level. The results were examined for correlations between the stress indicators, the number of copepods per fish and the life stage of the copepods. The presence of L. salmonis elevated stress indicators in relation to the specific sea lice stage. By day 21, both cortisol (mean 63.1 nmol L−1 controls: 179.8 nmol L−1 for parasitized) and glucose (mean 3.545 mmol L−1 controls: 4.567 mmol L−1 for parasitized) levels were significantly increased due to the presence of the lice. This was believed to be a direct result of the sea lice development into the larger life stages, thus increasing the level of host damage. 相似文献
998.
999.
1000.
Renata M. Barroso Paul A. Wheeler Scott E. LaPatra Robert E. Drew Gary H. Thorgaard 《Aquaculture (Amsterdam, Netherlands)》2008,277(3-4):156-163
Infectious hematopoietic necrosis virus (IHNV) is a major constraint to rainbow trout culture. Yellowstone cutthroat trout (Oncorhynchus clarki bouvieri) have greater resistance to this virus than do rainbow trout (O. mykiss), but the genetic mechanism of this resistance is not understood. We conducted a genome scan using a backcross of cutthroat trout into a rainbow trout background to estimate the number and locations of quantitative trait loci (QTL) associated with IHNV resistance and growth in trout. IHNV resistance was considered in terms of both survival (binary trait) and days to death (quantitative trait). The genetic map was scanned using interval mapping via two different approaches: one model considered survival alone and a second two-part model combined both survival and days to death. Three QTL were significantly (P ≤ 0.05) associated with virus resistance genome-wide, explaining 32.5% of the phenotypic variation. Cutthroat alleles at two of these QTL resulted in increased resistance to the pathogen, as expected. No growth QTL were detected in this cross. We suggest that these traits are genetically independent. 相似文献