全文获取类型
收费全文 | 214篇 |
免费 | 8篇 |
专业分类
林业 | 29篇 |
农学 | 5篇 |
基础科学 | 1篇 |
42篇 | |
综合类 | 17篇 |
农作物 | 15篇 |
水产渔业 | 17篇 |
畜牧兽医 | 86篇 |
园艺 | 3篇 |
植物保护 | 7篇 |
出版年
2023年 | 1篇 |
2022年 | 2篇 |
2021年 | 9篇 |
2020年 | 8篇 |
2019年 | 3篇 |
2018年 | 3篇 |
2017年 | 8篇 |
2016年 | 8篇 |
2015年 | 10篇 |
2014年 | 7篇 |
2013年 | 13篇 |
2012年 | 10篇 |
2011年 | 13篇 |
2010年 | 13篇 |
2009年 | 6篇 |
2008年 | 12篇 |
2007年 | 12篇 |
2006年 | 12篇 |
2005年 | 9篇 |
2004年 | 6篇 |
2003年 | 3篇 |
2002年 | 4篇 |
2001年 | 3篇 |
2000年 | 4篇 |
1999年 | 1篇 |
1998年 | 1篇 |
1996年 | 3篇 |
1993年 | 1篇 |
1992年 | 2篇 |
1991年 | 5篇 |
1990年 | 3篇 |
1989年 | 1篇 |
1988年 | 2篇 |
1987年 | 2篇 |
1986年 | 2篇 |
1982年 | 2篇 |
1981年 | 1篇 |
1979年 | 1篇 |
1977年 | 1篇 |
1973年 | 1篇 |
1972年 | 2篇 |
1971年 | 2篇 |
1970年 | 2篇 |
1969年 | 3篇 |
1968年 | 2篇 |
1966年 | 1篇 |
1965年 | 2篇 |
排序方式: 共有222条查询结果,搜索用时 171 毫秒
51.
von Bernuth H Picard C Jin Z Pankla R Xiao H Ku CL Chrabieh M Mustapha IB Ghandil P Camcioglu Y Vasconcelos J Sirvent N Guedes M Vitor AB Herrero-Mata MJ Aróstegui JI Rodrigo C Alsina L Ruiz-Ortiz E Juan M Fortuny C Yagüe J Antón J Pascal M Chang HH Janniere L Rose Y Garty BZ Chapel H Issekutz A Maródi L Rodriguez-Gallego C Banchereau J Abel L Li X Chaussabel D Puel A Casanova JL 《Science (New York, N.Y.)》2008,321(5889):691-696
MyD88 is a key downstream adapter for most Toll-like receptors (TLRs) and interleukin-1 receptors (IL-1Rs). MyD88 deficiency in mice leads to susceptibility to a broad range of pathogens in experimental settings of infection. We describe a distinct situation in a natural setting of human infection. Nine children with autosomal recessive MyD88 deficiency suffered from life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease. However, these patients were otherwise healthy, with normal resistance to other microbes. Their clinical status improved with age, but not due to any cellular leakiness in MyD88 deficiency. The MyD88-dependent TLRs and IL-1Rs are therefore essential for protective immunity to a small number of pyogenic bacteria, but redundant for host defense to most natural infections. 相似文献
52.
Fingerprinting sediment sources in the outlet reservoir of a hilly cultivated catchment in Tunisia 总被引:1,自引:1,他引:1
Abir Ben Slimane Damien Raclot Olivier Evrard Mustapha Sanaa Irène Lefèvre Mehdi Ahmadi Mouna Tounsi Cornelia Rumpel Abdallah Ben Mammou Yves Le Bissonnais 《Journal of Soils and Sediments》2013,13(4):801-815
Purpose
Approximately 74 % of agricultural soils in Tunisia are affected by water erosion, leading to the siltation of numerous human-made reservoirs and therefore a loss of water storage capacity. The objective of this study was to propose a methodology for estimating the relative contributions of gully/channel bank erosion and surface topsoil erosion to the sediment accumulated in small reservoirs.Materials and methods
We tested an approach based on the sediment fingerprinting technique for sediments collected from a reservoir (which has been in operation since 1994) at the outlet of a catchment (Kamech, 2.63 km2). Sampling concentrated on the soil surface (in both cropland and grassland), gullies and channel banks. A total of 17 sediment cores were collected along a longitudinal transect of the Kamech reservoir to investigate the origin of the sediment throughout the reservoir. Radionuclides (particularly caesium-137, 137Cs) and nutrients (total phosphorus, total nitrogen and total organic carbon (TOC)) were analysed as potential tracers.Results and discussion
The applications of a mixing model with 137Cs alone or 137Cs and TOC provided very similar results: The dominant source of sediment was surface erosion, which was responsible for 80 % of the total erosion within the Kamech catchment. Additionally, we showed that the analysis of a single composite core provided information on the sediment origin that was consistent with the analysis of all sediment layers in the core. We demonstrated the importance of the core sampling location within the reservoir for obtaining reliable information regarding sediment sources and the dominant erosion processes.Conclusions
The dominance of surface erosion processes indicates that conservation farming practices are required to mitigate erosion in the agricultural Kamech catchment. Based on the results from 17 sediment cores, guidelines regarding the number and location of sampling cores to be collected for sediment fingerprinting are proposed. We showed that the collection of two cores limited the sediment source apportionment uncertainty due to the core sampling scheme to <10 %. 相似文献53.
The genome sequence of the microsporidian parasite Encephalitozoon cuniculi Levaditi, Nicolau et Schoen, 1923 contains about 2,000 genes that are representative of a non-redundant potential proteome composed of 1,909 protein chains. The purpose of this review is to relate some advances in the characterisation of this proteome through bioinformatics and experimental approaches. The reduced diversity of the set of E. cuniculi proteins is perceptible in all the compilations of predicted domains, orthologs, families and superfamilies, available in several public databases. The phyletic patterns of orthologs for seven eukaryotic organisms support an extensive gene loss in the fungal clade, with additional deletions in E. cuniculi. Most microsporidial orthologs are the smallest ones among eukaryotes, justifying an interest in the use of these compacted proteins to better discriminate between essential and non-essential regions. The three components of the E. cuniculi mRNA capping apparatus have been especially well characterized and the three-dimensional structure of the cap methyltransferase has been elucidated following the crystallisation of the microsporidial enzyme Ecm1. So far, our mass spectrometry-based analyses of the E. cuniculi spore proteome has led to the identification of about 170 proteins, one-quarter of these having no clearly predicted function. Immunocytochemical studies are in progress to determine the subcellular localisation of microsporidia-specific proteins. Post-translational modifications such as phosphorylation and glycosylation are expected to be soon explored. 相似文献
54.
Kanika Singh Hitesh B. Vasava Didier Snoeck Bhabani S. Das David Yinil Damien Field Todd Sanderson Chris Fidelis Israr Majeed Niranjan Panigrahi 《Soil Use and Management》2019,35(3):492-502
In this study, diffuse reflectance spectroscopy (DRS) approach was examined for making input recommendations in the smallholder cocoa farms of Papua New Guinea (PNG). Soil samples were collected from four provinces of PNG. Soil samples from four different depths (0–10, 10–30, 30–60 and 60–90 cm) of 32 profiles in each of these site were used to create a database of soil chemical and physical properties. Spectral reflectance values at 1 nm interval covering visible to shortwave‐infrared (350–2,500 nm) were collected for each of these soil samples to develop partial least squares regression models. Soil textural fractions, soil organic carbon contents and available N were well predicted by the DRS approach with R2 values larger than 0.75. Moderate to poor estimation efficiencies were observed for remaining parameters. Nevertheless, the estimated soil attributes and their corresponding measured soil parameters were used as inputs to an input recommendation model of soil diagnosis to create input recommendation for a targeted cocoa yield of 1,000 kg dry cocoa beans ha‐1 Resulting input recommendations were similar for both of these input sources (measured and DRS‐estimated) suggesting that the DRS approach may provide an easy way to create input recommendations. 相似文献
55.
West NP Sansonetti P Mounier J Exley RM Parsot C Guadagnini S Prévost MC Prochnicka-Chalufour A Delepierre M Tanguy M Tang CM 《Science (New York, N.Y.)》2005,307(5713):1313-1317
Shigella, the leading cause of bacillary dysentery, uses a type III secretion system (TTSS) to inject proteins into human cells, leading to bacterial invasion and a vigorous inflammatory response. The bacterium is protected against the response by the O antigen of lipopolysaccharide (LPS) on its surface. We show that bacteriophage-encoded glucosylation of Shigella O antigen, the basis of different serotypes, shortens the LPS molecule by around half. This enhances TTSS function without compromising the protective properties of the LPS. Thus, LPS glucosylation promotes bacterial invasion and evasion of innate immunity, which may have contributed to the emergence of serotype diversity in Shigella. 相似文献
56.
Myllykallio H Lipowski G Leduc D Filee J Forterre P Liebl U 《Science (New York, N.Y.)》2002,297(5578):105-107
Although deoxythymidylate cannot be provided directly by ribonucleotide reductase, the gene encoding thymidylate synthase ThyA is absent from the genomes of a large number of nonsymbiotic microbes. We show that ThyX (Thy1) proteins of previously unknown function form a large and distinct class of thymidylate synthases. ThyX has a wide but sporadic phylogenetic distribution, almost exclusively limited to microbial genomes lacking thyA. ThyX and ThyA use different reductive mechanisms, because ThyX activity is dependent on reduced flavin nucleotides. Our findings reveal complexity in the evolution of thymidine in present-day DNA. Because ThyX proteins are found in many pathogenic microbes, they present a previously uncharacterized target for antimicrobial compounds. 相似文献
57.
Permanently cross-linked materials have outstanding mechanical properties and solvent resistance, but they cannot be processed and reshaped once synthesized. Non-cross-linked polymers and those with reversible cross-links are processable, but they are soluble. We designed epoxy networks that can rearrange their topology by exchange reactions without depolymerization and showed that they are insoluble and processable. Unlike organic compounds and polymers whose viscosity varies abruptly near the glass transition, these networks show Arrhenius-like gradual viscosity variations like those of vitreous silica. Like silica, the materials can be wrought and welded to make complex objects by local heating without the use of molds. The concept of a glass made by reversible topology freezing in epoxy networks can be readily scaled up for applications and generalized to other chemistries. 相似文献
58.
Gustavo Fernandes Viana Monica Midon Mariana Werneck Fonseca Alessandre Hataka Luciana Carandina Laís Melício Cintra Bueno José Nicolau Próspero Puoli Filho Vânia Maria de Vasconcelos Machado 《Anatomia, histologia, embryologia》2021,50(5):839-848
In humans and pets, magnetic resonance imaging (MRI) provides important adjunctive anatomic information about the thyroid and its adjacent structures. To the best of our knowledge, however, no studies have been reported regarding the use of MRI for thyroid evaluation in horses. The aim of this study was to obtain qualitative and quantitative parameters of healthy thyroid gland in adult horses by MRI. Eleven horses were submitted to thyroid MRI evaluation. The healthy thyroid of these animals was ensured by normal examinations performed before (free thyroxine and ultrasound) and after (cytology) to MRI. It was concluded that the dorsal spin-echo T1-weighted, fast spin-echo T2-weighted, sagittal STIR and a three-dimensional T1-weighted gradient echo were the sequences that best characterized the thyroid lobes. Qualitatively, there was a significant variation in the elliptical format of some lobes considering the sagittal plane, which oscillated between rounded and flattened conformation. Excellent reproducibility of the quantitative thyroid parameters obtained was verified. The thyroid gland total volume mean of observers was 14.31 cm3 (±0.69). In relation with comparisons between quantitative parameters of left and right lobes, only differences regarding their respective lengths were observed. Thus, we can infer that the difference in some thyroid lobes format can be explained by the significant variation identified between left and right lobes lengths. 相似文献
59.
Northern snake-necked turtles (Chelodina rugosa) traditionally provided an important seasonal source of protein for indigenous communities in Arnhem Land, northern Australia. Harvest techniques today differ little from those used historically, harvesting being applied in the late dry season when ephemeral waters have drawn down and turtles are aestivating. Radio-telemetry was used to quantify survival rates of C. rugosa at a traditional turtle harvest site and relate them to harvest, predation by feral pigs (Sus scrofa) and environmental factors. Although turtle survival was positively correlated with body size, the survival of turtles of all sizes and stages of maturity was compromised by pig predation. Seasonal variation in the onset, duration and severity of rainfall and associated influences on periodic drying, are important for C. rugosa survival because such variation influences the timing and intensity of both Aboriginal harvest and pig predation. Contemporary harvest rates of C. rugosa in Arnhem Land by Aboriginal people are very low because pig predation depletes available stocks immediately before Aboriginal harvesting. Aboriginal harvest rates are regulated also by the frequency and timing of ceremonies and other cultural activities that interfere with harvests. Before the arrival of pigs, such relaxation of harvest pressure in years when harvest would otherwise be possible would have contributed to the local abundance and persistence of C. rugosa. In contrast, pig predation is unrelenting, and years of high turtle survival are now restricted only to years of high wet season rainfall. 相似文献
60.