利用SCoT对甜菜种质资源的鉴定及遗传多样性分析

为了研究SCoT分子标记技术对甜菜种质资源鉴定的可行性。利用80条SCoT引物对48份甜菜种质资源进行鉴别,同时对种质资源进行了遗传多样性分析和亲缘关系的鉴定。结果表明,80条SCoT引物中有6条能够扩增出清晰、且多态性高的条带,分别为SCoT1、SCoT12、SCoT13、SCoT14、SCoT17和SCoT23,其中引物SCoT1、SCoT12、SCoT14和SCoT23单独使用均可鉴别全部的48份种质资源,引物SCoT13和SCoT17共同使用可以鉴别48个种质资源;聚类分析结果表明,在遗传距离0.15处,95.8%的种质资源均聚为一类,从分子角度上表明甜菜种质资源遗传距离较小。本研究为利用SCoT分子标记技术鉴别甜菜种质资源、对种质资源进行亲缘关系鉴定、杂交组合亲本选配以及分子标记辅助育种等提供相关科学依据。     

质核互作型不育系阜CMS5A大豆的选育及应用

阐述了质核互作型不育系阜CMS5A大豆的选育经过,总结了其特征特性,并分析了其应用成效,以供参考。     

云南白雪茶研究现状及开发利用

云南白雪茶是滇西北高寒地区民间特有的一种长期使用的药物和保健饮品,具有茶文化特征.从云南民间的食用经验来看,白雪茶具有显著的清热、止咳、安神等保健作用,现代药理学研究又证明,白雪茶还具有抗炎、解热、抗疲劳、抗辐射、促进免疫等药理学活性.根据雪茶的研究现状对其植物学特性、化学成分、保健作用作了简要的阐述,为以后的开发利用提供了理论依据.     

Identification and Molecular Characteristics Analysis of Porcine Epidemic Diarrhea Virus Variants

To ascertain a diarrhea case in a pig farm in Shandong province,the pathological changes of dead piglets were observed and nested RT-PCR test was carried out on 7 diarrhea samples for porcine epidemic diarrhea virus (PEDV).Pathological examination revealed that intestine was detected as enlargement,hyperemia and edema.After histological examination,the typical microscopic lesions of intestine were disappearance of epithelial cells,villus shrinkage and shortening.The result of nested RT-PCR showed that all of the 7 samples could amplify a specific target band of PEDV.Molecular characteristics of two field strains showed that they had an amino acid homology of 92.3% to 92.4% with vaccine CV777 and 96.6% to 98.6% with other previous field strains which sequences were downloaded from GenBank.Phylogenetic tree analysis further revealed that all of PEDV strains could be mainly divided into two clusters of G1 and G2. G2 consisted of the field strains of our study and other field strains from USA,China and so on,which had the same sequence characteristics of two insertions and one deletion,while G1 consisted of all vaccines of CV777 and several older field strains from China and Korea.These results indicated that our field strains were the dominant strains in recent epidemic diarrhea occurrence.Moreover,its molecular characteristics might be a characterization of differentiating the field and vaccine strains.     

Vitamin C enhanced myocardial differentiation of dedifferentiated fat cells

AIM: In order to observe the myocardial differentiation capacity of the dedifferentiated fat (DFAT) cells treated with vitamin C in vitro. METHODS: DFAT cells were dedifferentiated from the mature rat adipocytes with ceiling adherent culture. The DFAT cells of passage 3 were used in the study. Vitamin C and/or neonatal rat heart tissue lysate were added into the culture medium to induce myocardial differentiation for 3 weeks. The cell morphology was observed under microscope. The myocardial-specific markers, such as cTnT, GATA-4 and NKx2.5, were examined by the methods of immunofluorescence, PCR and Western blot. RESULTS: Mature rat adipocytes dedifferentiated into fibroblast-like DFAT cells after ceiling adherent culture. The DFAT cells spontaneously differentiated into cardiomyocyte-like cells under normal culture condition with a low incidence. After treated with neonatal rat heart cell lysate, the DFAT cells became cardiomyocyte-like cells that had bigger size, longer shape and myotubule-structure. The expression of cTnT, GATA-4 and NKx2.5 was remarkably increased at both mRNA and protein levels as compared with the normal cultured DFAT cells. The expression of cTnT, GATA-4 and NKx2.5 was further increased in DFAT cells after treating with vitamin C. No spontaneous beating cell was observed. CONCLUSION: Vitamin C enhances the differentiation of DFAT cells into cardiomyocyte-like cells.     

农业科研单位编制外用工的管理策略

编制外用工是农业科研单位普遍存在的用工形式。本文阐述了农业科研单位编制外用工的特点，分析了编制外用工存在问题及原因，提出从规范用工管理方式、强化考核管理、拓宽发展空间等方面提高编制外人员的工作积极性，提升编制外用工人力资源管理的效能，促进单位发展。     

基于GRA-TOPSIS耦合的草莓品质综合评价

研究不同水肥滴灌条件对草莓品质的综合影响,为草莓高品质生产提供水肥管理依据.以灌水量(X1)和施肥量(X2)为试验因子,依据蒸腾量(ETc)设置4个灌水水平(W1:100％ETc、W2:85％ETc、W3:70％ETc、W4:55％ETc);依据目标产量(F0)设置3个施肥水平(F1:120％F0、F2:100％F0、F3:80％F0),完全组合共12个处理.测定草莓可溶性总糖、可溶性蛋白、维生素C、可溶性固形物、糖酸比、游离氨基酸、还原性糖、硝酸盐8个品质指标,解析水肥耦合对各品质指标的调控效应.各品质指标对水肥因素响应不一致,基于博弈论组合赋权中,单一指标权重最大是可溶性蛋白为0.1689,维生素C次之为0.1551,硝酸盐最小为0.0918;基于GRA-TOPSIS建立草莓品质综合评价,解析草莓综合品质对水肥因素响应模型,可知,水肥交互作用显著,且施肥效应强于灌水.当灌水量控制在2 003～2566m3.hm-2,施肥量控制在1 878～2 195 kg.hm-2时,草莓综合品质最佳.     

吉林省蜜蜂疫病危害现状及发展对策

调查分析了吉林省主要蜜蜂饲养区蜜蜂疫病危害的现状,发现蜜蜂白垩病(56%)、蜂螨(40%)及爬蜂病(20%)为吉林省3大主要蜜蜂病害,同时通过实验室检测病蜂样本发现,蜜蜂微孢子虫的隐性感染情况较为严重,32%的蜂场存在不同程度的感染,应引起重视。蜜蜂敌害中胡蜂危害(42%)较为严重,个别蜂场兼受地胆、蚂蚁及蟾蜍的侵害。蜂病的确诊是目前饲养者面临的第一难题,建议尽快建立蜜蜂疫病防治技术体系,宣传蜜蜂健康养殖技术,加强流行病学及相关基础研究,加大抗病蜂种的培育力度,加快无公害对症蜂药的研究进展,给饲养者养成防患于未然、饲养强群等优良习惯,为蜜蜂产业的健康发展奠定良好的基础。